含Yunnanese(Aγδβ)°-缺失的染色体11中Gγ珠蛋白基因在MEL细胞中活跃表达

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-12-10 DOI:10.1360/ZB1995-25-12-1285

张俊武乔军宋文风邱志明, Jiao Jun Song Wen-Feng Qiu Zhi-Meng Zhang Dun-Wu

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引用次数: 0

摘要

用EB病毒转化技术获得了Yunnanese(Aγδβ)°-地中海贫血杂合子的淋巴细胞系,并用细胞融合法获得了其与鼠红白血病细胞(MEL)的杂合细胞,进一步筛选到仅含正常或异常人染色体11的次级克隆杂合细胞,并证明在只含正常人染色体11的次级克隆杂合细胞中Gγ,Aγ珠蛋白基因不表达,β珠蛋白基因表达,但在仅含具有缺失的人染色体11的杂合细胞中Gγ基因高水平表达。这些结果进一步确证在β-珠蛋白基因簇内的DNA片段缺失是导致Yunnanese(Aγδβ)°-地中海贫血中Gγ基因持续活跃表达的原因。

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含Yunnanese(Aγδβ)°-缺失的染色体11中Gγ珠蛋白基因在MEL细胞中活跃表达

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Science in China. Series B, Chemistry, life sciences & earth sciences

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