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Science in China. Series B, Chemistry, life sciences & earth sciences最新文献

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Interference of electromagnetic waves in dynamic metabolism. 电磁波对动态代谢的干扰。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-11-01
K Huang, J Tang, Y Liu, L Xu
{"title":"Interference of electromagnetic waves in dynamic metabolism.","authors":"K Huang,&nbsp;J Tang,&nbsp;Y Liu,&nbsp;L Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Life is a continuous process of the dynamic metabolism. The influence of electromagnetic waves on the process of metabolism cannot be neglected. Here a new theory of electromagnetic interference in the dynamic metabolism of life is proposed. The statistical dynamic equations of ion and free radical in the biochemical reaction radiated by electromagnetic waves are given. The intensity of electromagnetic interference could be described with an interference factor. Good agreement can be seen between the calculated and measured results for a famous experiment of radio-frequency radiation-induced calcium ion efflux enhancement.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 11","pages":"1355-60"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19719003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cloning, expression and purification of the ligand-binding region of human IL-6R in E. coli and its preliminary functional identification. 人IL-6R配体结合区在大肠杆菌中的克隆、表达、纯化及其初步功能鉴定。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-11-01
J Duan, J Wang, J Han, S Peng, M Zou, J Miao, C Zhao, X Ma
{"title":"Cloning, expression and purification of the ligand-binding region of human IL-6R in E. coli and its preliminary functional identification.","authors":"J Duan,&nbsp;J Wang,&nbsp;J Han,&nbsp;S Peng,&nbsp;M Zou,&nbsp;J Miao,&nbsp;C Zhao,&nbsp;X Ma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ligand-binding region of human IL-6R is taken as the target gene fragment to be cloned and expressed. With pET-3b as expressing vector, two recombinants pET-6R(B) and pET-6R(B)4 have been constructed encoding the ligand-binding region (28 kD) of hIL-6R and its dimmer (53 kD), respectively. After induction with IPTG, they produced two proteins rIL6R-28 of 28 kD and rIL6R-53 of 53 kD amounting to 50% and 30% of total bacteria proteins, respectively. The expressed products were mainly recovered as inclusion bodies. After purification and renaturation, both of them were capable of augmenting the growth-stimulating effect of IL-6 on 7TD1 cells, an IL-6 dependent cell line. The result of ELISA also revealed that both rIL6R-28 and rIL6R-53 had the obvious ligand-binding activity.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 11","pages":"1321-31"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19717742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cloning and expression of Kluyveromyces fragilis LAC4 gene. 脆弱克鲁维菌LAC4基因的克隆与表达。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-11-01
K Huo, Y Li
{"title":"Cloning and expression of Kluyveromyces fragilis LAC4 gene.","authors":"K Huo,&nbsp;Y Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The genomic library of Kluyveromyces fragilis was constructed in E. coli TG1, and 5 beta-galactosidase gene (LAC4) clones have been obtained from the library by complementation of the Kluyveromyces lactis lac4-8 mutation. The studies on the structure and the function of the LAC4 gene revealed that (i) the gene can also complement E. coli lacZ mutation; (ii) the physical map of the K. fragilis LAC4 gene was very similar to that of K. lactis; (iii) the beta-galactosidase levels expressed by the clone strains were much higher than that expressed by the original strain; (iv) the variation of the beta-galactosidase level of different clone strains induced by lactose or galactose was related to the retained degree of the 5' flanking region of LAC4 gene, suggesting that there might be a lactose specific transcription activating element in the region.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 11","pages":"1332-40"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19717743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
H 2 +NO→H+HNO( 3 A ″)选态反应动力学理论研究 H 2 +NO→H+HNO( 3 A ″)选态反应动力学理论研究
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-10-10 DOI: 10.1360/ZB1995-25-10-1026
李顺来 李宗和 冯文林, Li Zong-He Feng Wen-Lin Li Shun-Lai
{"title":"H 2 +NO→H+HNO( 3 A ″)选态反应动力学理论研究","authors":"李顺来 李宗和 冯文林, Li Zong-He Feng Wen-Lin Li Shun-Lai","doi":"10.1360/ZB1995-25-10-1026","DOIUrl":"https://doi.org/10.1360/ZB1995-25-10-1026","url":null,"abstract":"对H 2 +NO→H+HNO反应,用从头算方法(UHF/6-31G)做了IRC解析,计算活化能与实验值相符。用Miller的反应Hamilton理论进行了反应动态学分析,指出H 2 及NO振动模式可近似地被认为是可分离的模。并用Truhlar等的选态反应速率理论计算了高振动激发态的选态反应速率常数,获得与实验相近的结果。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"411 1","pages":"1026-1032"},"PeriodicalIF":0.0,"publicationDate":"1995-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73584758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
~(13)C-美沙西汀呼气法S参数诊断肝脏疾病的研究 ~(13)C-美沙西汀呼气法S参数诊断肝脏疾病的研究
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-10-10 DOI: 10.1360/ZB1995-25-10-1054
曾文炳 张维成 许士元 杨志忠 刘纯 朱德平 文启彬 申岐祥 王先彬
{"title":"~(13)C-美沙西汀呼气法S参数诊断肝脏疾病的研究","authors":"曾文炳 张维成 许士元 杨志忠 刘纯 朱德平 文启彬 申岐祥 王先彬","doi":"10.1360/ZB1995-25-10-1054","DOIUrl":"https://doi.org/10.1360/ZB1995-25-10-1054","url":null,"abstract":"以药物动力学模型的分析,明确地阐述了 13 C-美沙西汀呼气试验的机理,并详细报道了 13 C-美沙西汀呼气检查法的测量方法及其在肝脏疾病临床诊断上的应用。综合分析临床试验的数据,所提出的S诊断参数,对呼气检查法的应用和发展,具有重要意义。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"52 1","pages":"1054-1062"},"PeriodicalIF":0.0,"publicationDate":"1995-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80698142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
稀土元素异硫氰酸盐水合物,RE(NCS) 3 ·nH 2 O的低温热容和热力学性质的研究——II.Pr(NCS) 3 ·7H 2 O;Nd(NCS) 3 ·7H 2 O 和Nd(NCS) 3 ·7H 2 O 稀土元素异硫氰酸盐水合物,RE(NCS) 3 ·nH 2 O的低温热容和热力学性质的研究——II.Pr(NCS) 3 ·7H 2 O;Nd(NCS) 3 ·7H 2 O 和Nd(NCS) 3 ·7H 2 O
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-10-10 DOI: 10.1360/ZB1995-25-10-1033
谭志诚 松尾隆佑 菅宏 张志英 尹敬执 蒋本杲 孙同山
{"title":"稀土元素异硫氰酸盐水合物,RE(NCS) 3 ·nH 2 O的低温热容和热力学性质的研究——II.Pr(NCS) 3 ·7H 2 O;Nd(NCS) 3 ·7H 2 O 和Nd(NCS) 3 ·7H 2 O","authors":"谭志诚 松尾隆佑 菅宏 张志英 尹敬执 蒋本杲 孙同山","doi":"10.1360/ZB1995-25-10-1033","DOIUrl":"https://doi.org/10.1360/ZB1995-25-10-1033","url":null,"abstract":"用全自动绝热量热计测定了Pr(NCS) 3 ·7H 2 O;Nd(NCS) 3 ·7H 2 O 和Nd(NCS) 3 ·7H 2 O 13～300K温区热容,发现Pr(NCS) 3 ·7H 2 O在50K以下具有Schottky反常热容。根据实验热容数据用最小二乘拟合方法得出了计算这两种化合物13～300K温区内热容值的多项式方程。13K以下的热容用Debye-Einstein热容函数和Schottky热容函数进行了估算。根据热容数据计算出了这两种物种0～300K温区的标准热力学函数,并结合文献数据计算出了标准生成熵和标准生成Gibbs能。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"71 1","pages":"1033-1040"},"PeriodicalIF":0.0,"publicationDate":"1995-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85899269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
氢键的精确从头计算方法及其用于NH 3 ,H 2 O和HF分子间氢键的研究 氢键的精确从头计算方法及其用于NH 3 ,H 2 O和HF分子间氢键的研究
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-10-10 DOI: 10.1360/ZB1995-25-10-1016
王一波 陶福明 潘毓刚, Dao Fu-Meng Bo Yu-Gang Wang Yi-Bei
{"title":"氢键的精确从头计算方法及其用于NH 3 ,H 2 O和HF分子间氢键的研究","authors":"王一波 陶福明 潘毓刚, Dao Fu-Meng Bo Yu-Gang Wang Yi-Bei","doi":"10.1360/ZB1995-25-10-1016","DOIUrl":"https://doi.org/10.1360/ZB1995-25-10-1016","url":null,"abstract":"提出一种用二级Moller-Plesset微扰理论MP2,结合中点键函数和基函数重叠误差BSSE完全均衡校正的从头计算方法,精确地计算氢键的结合能和结构,使计算结果较快地稳定收敛到完全基极限,接近实验精度。同时,阐述了电子相关效应、基函数及BSSE、键函数效果及使用条件和分子间几何构型优化判据等问题。还用此方法精确地研究了氢键体系HOH…NH 3 ,FH…NH 3 和H 2 O…HF的结构和能量性质,获得了较前人更好的、对实验研究颇有参考价值的理论结果。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"9 1","pages":"1016-1025"},"PeriodicalIF":0.0,"publicationDate":"1995-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73382578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
大别造山带冷却年龄的 40 Ar- 39 Ar和裂变径迹年龄测定 大别造山带冷却年龄的 40 Ar- 39 Ar和裂变径迹年龄测定
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-10-10 DOI: 10.1360/ZB1995-25-10-1086
陈江峰, 谢智, 刘顺生, 李学明, K.A.Foland
{"title":"大别造山带冷却年龄的 40 Ar- 39 Ar和裂变径迹年龄测定","authors":"陈江峰, 谢智, 刘顺生, 李学明, K.A.Foland","doi":"10.1360/ZB1995-25-10-1086","DOIUrl":"https://doi.org/10.1360/ZB1995-25-10-1086","url":null,"abstract":"应用同一岩石样品的角闪石、黑云母的 40 Ar- 39 Ar和磷灰石的裂变径迹定年结果,讨论了大别造山带岩石的冷却历史。南大别岩石在约190Ma前,北大别岩石在约125Ma前冷却到约540℃,冷却速率先快后慢,在约110～120Ma前南、北大别岩石都冷却约100℃,表明南、北大别地块间以及与超高压变质岩石间都有相对垂直运动,直到约110～120Ma前才结束相对运动。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"19 1","pages":"1086-1092"},"PeriodicalIF":0.0,"publicationDate":"1995-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80213123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
球形烯顺-反异构体的 1 H NMR研究 球形烯顺-反异构体的 1 H NMR研究
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-10-10 DOI: 10.1360/ZB1995-25-10-1041
姜月顺 小山泰, Xiao Shan-Tai Jiang Ru-Shun
{"title":"球形烯顺-反异构体的 1 H NMR研究","authors":"姜月顺 小山泰, Xiao Shan-Tai Jiang Ru-Shun","doi":"10.1360/ZB1995-25-10-1041","DOIUrl":"https://doi.org/10.1360/ZB1995-25-10-1041","url":null,"abstract":"从Rhodobacter sphaeroides 2.4.1中提取全反式球形烯,用高压液相色谱分离其I_2增感异构化所产生的顺-反异构体。解析全反式球形烯的 1 H NMR谱(COSY,long-range COSY,relayed COSY,ROESY,NOESY)的基础上,根据共轭双键区=CH的异构化位移确定顺式异构体的结构。按HPLC溶出顺序,A:9,13′-cis,B 1 :5,13-cis,B 2 :13,9′-cis,C:13-cis,E:9-cis,F:13′-cis,G:5,9′-cis,H:9′-cis,I:all-trans。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"722 1","pages":"1041-1048"},"PeriodicalIF":0.0,"publicationDate":"1995-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85803225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cloning, expression and characterization of a single-chain antibody PS-9 targeted to pancreatic cancer. 靶向胰腺癌单链抗体PS-9的克隆、表达和鉴定
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-10-01
X Yan, B Tien, L Li, M Yuan
{"title":"Cloning, expression and characterization of a single-chain antibody PS-9 targeted to pancreatic cancer.","authors":"X Yan,&nbsp;B Tien,&nbsp;L Li,&nbsp;M Yuan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Genes encoding single-chain antibodies have been first constructed, which consist of the heavy and light chain variable domains of antibody PS-9 joined together by a flexible peptide linker. The genes were cloned into coat protein g3p genes of pCANTAB5 phagemids, and expressed as fusion proteins on the phage tips. Immunological assay demonstrated that the engineered antibodies specifically bound to cancer cells LS-174-T as well as to pure bovine submaxillary gland mucin. Their specificity and affinity appeared the same as their parent antibodies. Our results supposed that the single-chain antibodies will be a target for the diagnosis and treatment of cancer.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 10","pages":"1230-6"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19535542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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