ObesityPub Date : 2025-06-29DOI: 10.1002/oby.24323
Marjorie Reyes-Farias, Pablo Fernández-García, Patricia Corrales, Lorena González, David Navarro-Sanagustín, Andrea Soria-Gondek, Silvia Pellitero, Jordi Tarascó, Pau Moreno, José M. Balibrea, Laia Gatell, Lauro Sumoy, María Galán, Ester Martínez, Francesc Villarroya, Rubén Cereijo, Laura Herrero, David Sánchez-Infantes
{"title":"Lipid-associated macrophages are more abundant in subcutaneous than visceral adipose tissue in patients with obesity","authors":"Marjorie Reyes-Farias, Pablo Fernández-García, Patricia Corrales, Lorena González, David Navarro-Sanagustín, Andrea Soria-Gondek, Silvia Pellitero, Jordi Tarascó, Pau Moreno, José M. Balibrea, Laia Gatell, Lauro Sumoy, María Galán, Ester Martínez, Francesc Villarroya, Rubén Cereijo, Laura Herrero, David Sánchez-Infantes","doi":"10.1002/oby.24323","DOIUrl":"10.1002/oby.24323","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Because white adipose tissue is infiltrated by several immune cells and their signature in individuals with obesity has not been fully characterized, we wanted to study the most abundant population, which is macrophages, a subtype of myeloid cell.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>To address this objective, we performed transcriptomic analysis of subcutaneous adipose tissue (SAT)- and visceral adipose tissue (VAT)-infiltrated CD11b<sup>+</sup> myeloid cells from individuals with severe obesity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our results showed that gene expression in human white adipose tissue–infiltrated CD11b<sup>+</sup> myeloid cells was depot-dependent. The expression of lipid-associated macrophage biomarkers was higher in SAT- than VAT-infiltrated CD11b<sup>+</sup> cells (<i>TREM2, CD9, GPNMB, CD68</i>). In contrast, VAT-infiltrated CD11b<sup>+</sup> cells overexpressed genes associated with a perivascular M2-like adipose tissue macrophage signature (<i>LYVE1, TIMD4, MRC1</i>). In addition, no classical gene expression polarization (M1 and M2) was shown when VAT and SAT CD11b<sup>+</sup> cells were compared. Finally, high levels of <i>CD248</i>, a sensor of lipids associated with insulin resistance, were found to be overexpressed in SAT- compared with VAT-infiltrated CD11b<sup>+</sup> myeloid cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study characterizes for the first time the macrophage biomarker signature in human VAT- and SAT-infiltrated CD11b<sup>+</sup> myeloid cells from individuals with severe obesity. Further studies are required to elucidate their potential role and specific function in the immunometabolism of individuals with obesity.</p>\u0000 </section>\u0000 </div>","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 8","pages":"1543-1554"},"PeriodicalIF":4.2,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/oby.24323","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ObesityPub Date : 2025-06-25DOI: 10.1002/oby.24346
Redin A. Spann, Christopher D. Morrison, Hans-Rudolf Berthoud
{"title":"Role of protein leverage versus hedonics in overeating and weight gain on palatable diet","authors":"Redin A. Spann, Christopher D. Morrison, Hans-Rudolf Berthoud","doi":"10.1002/oby.24346","DOIUrl":"10.1002/oby.24346","url":null,"abstract":"","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 7","pages":"1213-1214"},"PeriodicalIF":4.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ObesityPub Date : 2025-06-22DOI: 10.1002/oby.24345
Robert F. Kushner, Donna H. Ryan, Nina M. Harder-Lauridsen, Ole Kleist Jeppesen, Klaus Kallenbach, A. Michael Lincoff, Ildiko Lingvay
{"title":"Response to “Weight-loss drugs like Wegovy could lead to an increased risk of fragility fractures in population at risk”","authors":"Robert F. Kushner, Donna H. Ryan, Nina M. Harder-Lauridsen, Ole Kleist Jeppesen, Klaus Kallenbach, A. Michael Lincoff, Ildiko Lingvay","doi":"10.1002/oby.24345","DOIUrl":"10.1002/oby.24345","url":null,"abstract":"","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 8","pages":"1412-1413"},"PeriodicalIF":4.2,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fat taste sensitivity in women with obesity: transcriptomic analysis of fungiform papillae before and after bariatric surgery","authors":"Arnaud Bernard, Corentin Richard, Loredana Radoi, Romain Boidot, Séverine Ledoux, Philippe Besnard","doi":"10.1002/oby.24325","DOIUrl":"10.1002/oby.24325","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Fat taste sensitivity has been implicated in long-term weight loss after bariatric surgery. Unexpectedly, improvement of orosensory fat acuity was not systematic in operated patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Obesity-induced alterations of gene expression in gustatory papillae having been recently reported, we have explored the fungiform transcriptomic activity in the bariatric surgery context. Fat taste detection thresholds (three-alternative ascending forced-choice tests) alongside transcriptomic profiling of freshly isolated fungiform papillae were assessed in women with severe obesity before and 6 months after sleeve gastrectomy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>According to evolution of post surgery detection thresholds, lipid-improved and -unimproved subgroups were identified, despite similar initial anthropometric parameters. Lower weight loss and higher plasma fasting insulin, C-reactive protein, and fibrinogen levels distinguished lipid-unimproved patients. This subgroup also overexpressed genes involved in inflammation and apoptosis, with certain ones being positively correlated with high lipid detection thresholds (low sensitivity), whereas the key gene of taste bud cell signaling, <i>TRPM5</i>, was down-expressed. In lipid-improved patients, genes implicated in cell renewal, neuronal function, and receptor recycling were upregulated and associated with better lipid detection efficiency. Pathway enrichment identified immune and anti-inflammatory activities in lipid-unimproved and -improved groups, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings collectively suggest that changes in gene expression within gustatory papillae contribute to fat taste sensitivity after sleeve gastrectomy.</p>\u0000 </section>\u0000 </div>","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 8","pages":"1518-1528"},"PeriodicalIF":4.2,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/oby.24325","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ObesityPub Date : 2025-06-19DOI: 10.1002/oby.24341
Léa Karam, Julien Paccou
{"title":"Weight loss drugs like Wegovy could lead to an increased risk of fragility fractures in population at risk","authors":"Léa Karam, Julien Paccou","doi":"10.1002/oby.24341","DOIUrl":"10.1002/oby.24341","url":null,"abstract":"","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 8","pages":"1410-1411"},"PeriodicalIF":4.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ObesityPub Date : 2025-06-18DOI: 10.1002/oby.24294
Zheng Hao Chen, Gabrielle Jacobson, Myrtha E. Reyna, Paula Parvulescu, Russell J. de Souza, Mark R. Palmert, Wendy Lou, Susan C. Campisi, Elinor Simons, Stuart E. Turvey, Theo J. Moraes, Piushkumar J. Mandhane, Padmaja Subbarao, Kozeta Miliku
{"title":"Associations of dietary patterns and obesity development in school-aged children: results from the CHILD Cohort Study","authors":"Zheng Hao Chen, Gabrielle Jacobson, Myrtha E. Reyna, Paula Parvulescu, Russell J. de Souza, Mark R. Palmert, Wendy Lou, Susan C. Campisi, Elinor Simons, Stuart E. Turvey, Theo J. Moraes, Piushkumar J. Mandhane, Padmaja Subbarao, Kozeta Miliku","doi":"10.1002/oby.24294","DOIUrl":"10.1002/oby.24294","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>We aimed to understand data-driven dietary patterns in Canadian preschoolers and their impact on obesity development among male and female individuals.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In the prospective, population-based Canadian pregnancy cohort, the CHILD Cohort Study (<i>N</i> = 2219), dietary intake was assessed at age 3 years using a previously developed 112-item food frequency questionnaire. At age 5 years, we measured height, weight, and waist circumference and calculated BMI and waist circumference <i>z</i> scores. Obesity was defined as BMI <i>z</i> score > 2. We used principal components analysis to derive dietary patterns and multivariable-adjusted regression analyses to determine dietary patterns' associations with BMI and waist circumference <i>z</i> scores, as well as obesity status.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among Canadian preschoolers, we identified three dietary patterns: “Prudent” (high in vegetables, fruits, legumes, and fish); “Western-like” (high in fast foods, red/processed meats, and carbonated drinks); and “Refined Grain-Snack” (high in refined grains, dairy, and salty snacks). At age 5 years, 4.7% of the children were living with obesity (3.1% male individuals and 1.6% female individuals). Females adhering to the Refined Grain-Snack pattern had higher waist circumference <i>z</i> scores (β = 0.14; 95% CI: 0.03–0.25) and 2.74-fold odds of living with obesity (95% CI: 1.29–5.85). No significant associations were observed among male individuals or with other dietary patterns and obesity outcomes among female individuals.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Preschool dietary patterns are associated with sex-biased obesity development, highlighting the need for further research to explore these differences and inform targeted obesity prevention strategies during this important developmental period.</p>\u0000 </section>\u0000 </div>","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 7","pages":"1355-1364"},"PeriodicalIF":4.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/oby.24294","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144319073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ObesityPub Date : 2025-06-17DOI: 10.1002/oby.24297
Cindy W. Leung, Alessandra Uriarte, Minal R. Patel, Markell Miller, Eileen Spring, Julia A. Wolfson, Alicia J. Cohen, Michele Heisler, Wei Hao
{"title":"Food insecurity and the risk of diabetes: understanding the role of BMI as a mediator and moderator","authors":"Cindy W. Leung, Alessandra Uriarte, Minal R. Patel, Markell Miller, Eileen Spring, Julia A. Wolfson, Alicia J. Cohen, Michele Heisler, Wei Hao","doi":"10.1002/oby.24297","DOIUrl":"10.1002/oby.24297","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This retrospective cohort study examined BMI as a mediator and moderator of the association between food insecurity and diabetes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Data came from the electronic health records of 74,174 primary care patients at a large academic medical center. We used multivariate Poisson regression models to examine the association between food insecurity and diabetes. We used causal mediation analysis to evaluate the direct and indirect effects by which BMI mediates and moderates this association and the extent to which these effects varied by age, sex, and race and ethnicity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>During the 5-year period, 6.2% of patients were newly diagnosed with diabetes. Food insecurity was associated with a higher risk of diabetes (relative risk [RR] 1.19, 95% CI: 1.03–1.36) after multivariate adjustment. In mediation analysis, BMI was a significant mediator of the association between food insecurity and diabetes (natural indirect effect: RR 1.10, 95% CI: 1.06–1.14). When accounting for an interaction with BMI, BMI remained a significant mediator (natural indirect effect: RR 1.12, 95% CI: 1.0–1.17). In subgroup analyses, the mediator–moderator effect of BMI was stronger among adults <45 years old and female patients; there were no differences by race or ethnicity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our findings support BMI as a mediator and moderator of the association between food insecurity and diabetes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 7","pages":"1375-1385"},"PeriodicalIF":4.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144319074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ObesityPub Date : 2025-06-17DOI: 10.1002/oby.24287
M. Jennifer Abuzzahab, Beatrice Dubern, Anthony P. Goldstone, Andrea M. Haqq, Steven B. Heymsfield, Jennifer L. Miller, Jesse Richards, Martin Wabitsch, Jack A. Yanovski
{"title":"Improving the diagnosis of hyperphagia in melanocortin-4 receptor pathway diseases","authors":"M. Jennifer Abuzzahab, Beatrice Dubern, Anthony P. Goldstone, Andrea M. Haqq, Steven B. Heymsfield, Jennifer L. Miller, Jesse Richards, Martin Wabitsch, Jack A. Yanovski","doi":"10.1002/oby.24287","DOIUrl":"10.1002/oby.24287","url":null,"abstract":"<p>Characteristics of hyperphagia include heightened and prolonged hunger, longer time to satiation, shorter duration of satiety, severe preoccupation with food (i.e., hyperphagic drive), abnormal food-seeking behaviors, and distress or functional impairment when food is unavailable. Patients with melanocortin-4 receptor (MC4R) pathway diseases including those caused by variants in one of multiple key genes of the pathway often present with hyperphagia that results in early-onset, severe obesity because this pathway plays a critical role in regulation of hunger/satiation and energy balance. Patients with syndromic obesity (e.g., Bardet-Biedl syndrome) may also have hyperphagia as a result of neurodevelopmental disruptions in the MC4R pathway. Genetic testing is suggested in patients with early-onset, severe obesity and clinical features of genetic obesity (e.g., hyperphagia, neurodevelopmental differences, dysmorphic features); however, only a small percentage of individuals who meet these criteria undergo testing, potentially owing to limited availability, overlapping symptoms with other obesity types, and infrequent use of genetic testing during diagnosis. Diagnosing hyperphagia may be challenging, as no guidelines have been established for individuals with MC4R pathway diseases. Identifying these individuals is crucial to addressing the challenges of hyperphagia and associated obesity, which often limit quality of life and place overwhelming burdens on patients and families.</p>","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 7","pages":"1217-1231"},"PeriodicalIF":4.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/oby.24287","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144319075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ObesityPub Date : 2025-06-16DOI: 10.1002/oby.24320
Sally M. Vanegas, Silvia Curado, Boyan Zhou, Nicholas Illenberger, Ericka N. Merriwether, Evelyn Armijos, Ann Marie Schmidt, Christine Ren-Fielding, Manish Parikh, Brian Elbel, José O. Alemán, Melanie Jay
{"title":"Variations in weight loss and glycemic outcomes after sleeve gastrectomy by race and ethnicity","authors":"Sally M. Vanegas, Silvia Curado, Boyan Zhou, Nicholas Illenberger, Ericka N. Merriwether, Evelyn Armijos, Ann Marie Schmidt, Christine Ren-Fielding, Manish Parikh, Brian Elbel, José O. Alemán, Melanie Jay","doi":"10.1002/oby.24320","DOIUrl":"10.1002/oby.24320","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study examined racial and ethnic differences in percent total weight loss (%TWL) and glycemic improvement following sleeve gastrectomy (SG) and explored the role of socioeconomic and psychosocial factors in postsurgical outcomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This longitudinal study included patients who underwent SG between 2017 and 2020, with follow-up visits over 24 months.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Non-Hispanic Black (NHB) participants had lower %TWL at 3, 12, and 24 months compared with Hispanic (H) and non-Hispanic White (NHW) participants. Fat mass index was initially lower in NHB, with smaller reductions over time and significant group differences persisting at 24 months. NHB participants had higher baseline fat-free mass index values; by 24 months, fat-free mass index values were lower in H participants. Hemoglobin A1c decreased across all groups but remained consistently higher in NHB and H compared with NHW at 24 months. NHB participants reported higher perceived discrimination, sleep disturbance, and perceived stress than H and NHW participants at all time points. Employment status predicted %TWL at 12 months. There was a significant interaction between race and ethnicity and employment status observed at 12 and 24 months, suggesting that employment-related disparities could impact surgical outcomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>NHB participants experienced less favorable outcomes following SG, emphasizing the need for tailored interventions addressing socioeconomic and psychosocial disparities.</p>\u0000 </section>\u0000 </div>","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 8","pages":"1567-1579"},"PeriodicalIF":4.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ObesityPub Date : 2025-06-16DOI: 10.1002/oby.24321
Andressa França Sousa Bispo, Jussara de Jesus Simao, Miguel Ambrizzi Moraes, Ana Beatriz Marques Abel, Victor Tadeu Gonçalves Plata, Monica Marques Telles, André Valente Santana, Paula Volpe, Lucia Maria Armelin-Correa, Maria Isabel Cardoso Alonso-Vale
{"title":"Effects of obesity-associated plasma markers on adipose stem cell function and epigenetic regulation","authors":"Andressa França Sousa Bispo, Jussara de Jesus Simao, Miguel Ambrizzi Moraes, Ana Beatriz Marques Abel, Victor Tadeu Gonçalves Plata, Monica Marques Telles, André Valente Santana, Paula Volpe, Lucia Maria Armelin-Correa, Maria Isabel Cardoso Alonso-Vale","doi":"10.1002/oby.24321","DOIUrl":"10.1002/oby.24321","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study investigates the correlations between obesity-related plasma markers and epigenetic/inflammatory changes in white adipose tissue (WAT), focusing on adipose-derived stem cells (ASCs). We hypothesize that obesity modulates histone H3K27 marks, modified by demethylases (lysine-specific demethylase 6A and 6B [KDM6A/KDM6B]) and acetylases (CREB–binding protein [CREBBP]/histone acetyltransferase EP300), affecting ASC function.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Serum and visceral WAT (omental region) was collected from male patients (<i>n</i> = 16, 30–50 years old) undergoing elective gastric or bariatric surgery. BMI and obesity markers were correlated with changes in ASCs (transcript expression, proliferation, and secretion) using reverse transcriptase-polymerase chain reaction.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>ASCs from individuals with higher BMI exhibited slower proliferation, increased inflammatory profile, and reduced adipogenic potential, with lower expression of key adipogenic genes. H3K27 acetylase transcripts were also negatively correlated with adipogenesis regulators. Moreover, C-C motif chemokine 2 (CCL2) and KDM6A expression was higher in the group with obesity, as were CREBBP and EP300. Finally, leptin levels positively correlated with serum, WAT, and ASC CCL2 expression. In vitro, leptin exposure enhanced CCL2 expression/secretion and increased KDM6A/KDM6B and EP300 transcription.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In vitro leptin exposure enhanced CCL2 expression/secretion and increased KDM6A/KDM6B and EP300 transcription, highlighting how obesity-driven epigenetic mechanisms, including leptin-mediated pathways, disrupt ASC plasticity and perpetuate adipose tissue dysfunction, offering novel therapeutic targets for metabolic disease intervention.</p>\u0000 </section>\u0000 </div>","PeriodicalId":215,"journal":{"name":"Obesity","volume":"33 8","pages":"1529-1542"},"PeriodicalIF":4.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/oby.24321","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}