Saudi Journal of Biological Sciences最新文献

{"title":"In vitro anticancer, antioxidant and antibacterial activities of crude extract prepared from Enteromorpha intestinalis habited in Jeddah, Saudi Arabia","authors":"Bandar A. Al-Mur","doi":"10.1016/j.sjbs.2024.104026","DOIUrl":"https://doi.org/10.1016/j.sjbs.2024.104026","url":null,"abstract":"<div><p>The recent study purposes to evaluate the biological activities of <em>Enteromorpha intestinalis</em> gathered from Jeddah coastal area, Saudi Arabia, with respect to its phytochemical components. Our results indicated that the values ​​of moisture content, ash, total organic matter, total proteins, total lipids and total carbohydrates were 34.25 ± 5.6 %, 40.70 ± 2.3 %, 25.05 ± 1.73 %, 14.39 ± 0.8 %, 4.86 ± 6.9 % and 2.81 ± 1.4 %, respectively. The data also showed that the total phenols and flavonoids were 345.04 ± 1.50 and 320.67 ± 0.92 mg/g in the dried sample, respectively. Furthermore, four compounds were detected by HPLC at very low concentrations (quinic acid, ellagic acid, cinnamic acid, and phenanthrene) and flavonoids data confirmed the presence of apeginin, rudin, diosmin, and quercilin at high concentrations of 141.26, 11.42, 121.75, and 145.28. mg/g, respectively. The crude extract of <em>Enteromorpha intestinalis</em> exhibited cytotoxicity toward hepatocellular carcinoma cells (HepG-2 cell line) using an MTT assay with concentration range between 2 and 500 µg/mL for 48 h with IC_{50 =} 40.02 ± 3.94 µg/mL. Evidently, the <em>Enteromorpha intestinalis</em> extract had Hepatoprotective activity with IC₅₀ = 447.31 ± 14.59 μg/mL. The IC₅₀ activity of a crude methanol extract of <em>Enteromorpha intestinalis</em> was compared with that of an antioxidant drug (Torolox). The value (98.82 ± 1.30 μg/mL) was recorded close to Torolox (62.4 ± 0.70 μg/mL). This extract also possessed moderate antibacterial activity with inhibition zones ranging between 10 mm against <em>Pseudomonas aeruginosa</em> to 16 mm against <em>Escherichia coli</em>. <strong>Green seaweed, along with other types of seaweed, has received significant attention in recent years. Despite their potential benefits, green seaweeds are underutilized in many parts of the world. Extensive studies on different green seaweed isolates and extracts are necessary.</strong></p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104026"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001049/pdfft?md5=54a9642edd77c017759595e878cac117&pid=1-s2.0-S1319562X24001049-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141097583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survivin: A key apoptosis inhibitor in COVID-19 infection and its implication for treatment protocol","authors":"Faris Q.B. Alenzi","doi":"10.1016/j.sjbs.2024.104021","DOIUrl":"10.1016/j.sjbs.2024.104021","url":null,"abstract":"<div><p>While the relationship between cellular apoptosis and proliferation rates in COVID patients remains underexplored in existing literature, various viruses are known to impact these fundamental process to modulate response to infection. This paper aims to assess apoptosis and proliferation rates in individuals recently infected with Coronavirus, both before and after vaccination, comparing them with healthy controls. Peripheral blood cells from newly diagnosed COVID-19 patients revealed a significant increase in proliferation and apoptosis levels in fresh lymphocytes and granulocytes compared to healthy donors. Notably, as none of the patients were under corticosteroid therapy or cytotoxic drugs, the study underscores the critical role of white blood (WBC) apoptosis in viral pathogenesis, potentially contributing significantly to COVID-19′s pathogenicity. Elevated levels of soluble Fas ligand (FaSL) and the pro-inflatmmatory cytokine IL-38 were identified in COVID-19 patients, indicating potential immune dysregulation. Furthermore, individual who received the vaccine or recovered from COVID-19 exhibited higher survivin rates, suggesting a protective role for survivin in migitating lung damage. These findings suggest the prospect of developing a strategy to prevent WBC apoptosis, offering potential benefits in averting lymphopenia associated with severe COVID-19 ouctomes.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104021"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24000998/pdfft?md5=dbb323326d298f9812b5188340eae1b5&pid=1-s2.0-S1319562X24000998-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141055450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenesis of COVID19 and the applications of US FDA-approved repurposed antiviral drugs to combat SARS-CoV-2 in Saudi Arabia: A recent update by review of literature","authors":"Almonther Abdullah Hershan","doi":"10.1016/j.sjbs.2024.104023","DOIUrl":"https://doi.org/10.1016/j.sjbs.2024.104023","url":null,"abstract":"<div><p>Still, there is no cure for the highly contagious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-caused coronavirus disease 2019 (COVID19). The COVID19 pandemic caused health emergencies which resulted in enormous medical and financial consequences worldwide including Saudi Arabia. Saudi Arabia is the largest Arab country of the Middle East. The urban setting of Saudi Arabia makes it vulnerable towards SARS-CoV-2 (SCV-2). Religious areas of this country are visited by millions of pilgrims every year for the Umrah and Hajj pilgrimage, which contributes to the potential COVID19 epidemic risk. COVID19 throws various challenges to healthcare professionals to choose the right drugs or therapy in clinical settings because of the lack of availability of newer drugs. Current drug development and discovery is an expensive, complex, and long process, which involves a high failure rate in clinical trials. While repurposing of United States Food and Drug Administration (US FDA)-approved antiviral drugs offers numerous benefits including complete pharmacokinetic and safety profiles, which significantly shorten drug development cycles and reduce costs. A range of repurposed US FDA-approved antiviral drugs including ribavirin, lopinavir/ritonavir combination, oseltamivir, darunavir, remdesivir, nirmatrelvir/ritonavir combination, and molnupiravir showed encouraging results in clinical trials in COVID19 treatment. In this article, several COVID19-related discussions have been provided including emerging variants of concern of, COVID19 pathogenesis, COVID19 pandemic scenario in Saudi Arabia, drug repurposing strategies against SCV-2, as well as repurposing of US FDA‐approved antiviral drugs that might be considered to combat SCV-2 in Saudi Arabia. Moreover, drug repurposing in the context of COVID19 management along with its limitations and future perspectives have been summarized.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104023"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001013/pdfft?md5=6bb227e2103416a60916ca58f400477a&pid=1-s2.0-S1319562X24001013-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140951208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and oxidative stress responses in a cage designed for creep-feeding lambs","authors":"Fatih Yildirim ,&nbsp;Ahmet Yildiz ,&nbsp;Burak Hülagü ,&nbsp;Ali Osman Kesen ,&nbsp;Alperen Varalan ,&nbsp;Tuba Doğan","doi":"10.1016/j.sjbs.2024.104007","DOIUrl":"https://doi.org/10.1016/j.sjbs.2024.104007","url":null,"abstract":"<div><p>This research aims to assess the production and oxidative stress reactions of lambs fed in a specially designed cage for creep feeding. For this purpose, the Akkaraman lambs (n = 60) were separated into four groups (15 individuals by two sexes and two groups), and all were fed the same ration. On the days that all the data was collected, the lambs were precisely 20, 34, 48, and 62 days old. The study included collecting data on body weights, body measures, and saliva samples from lambs. When the change in body weight was investigated, the results indicated that the differences between caged creep and control feeding groups (P = 0.343) and among the groupings concerning gender (P = 0.735) were insignificant. The importance levels of change differences in body measurements based on feeding methods and gender are withers height: P = 0.003, P = 0.198; body length: P &lt; 0.001, P = 0.394; pectoral chest width: P = 0.030, P = 0.906; chest depth: P &lt; 0.001, P = 0.741; chest circumference: P = 0.093, P = 0.529; back length: P = 0.221, P = 0.935; head length: P = 0.004, P = 0.072 and head width: P = 0.112, P = 0.617 calculated. The study's saliva samples, the effects were examined, and significant differences in Malondialdehyde (P = 0.014), superoxide dismutase (P = 0.029), catalase (P &lt; 0.001), and glutathione s-transferase (P = 0.001) were discovered between control feeding and caged creep feeding methods. In ischemia-modified albumin (P &gt; 0.05), the difference between the groups was insignificant. According to these findings, caged creep feeding systems are preferable for achieving faster growth rates in Akkaraman lambs; however, caged creep feeding techniques in bigger areas with longer periods are considered better for welfare conditions.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104007"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24000858/pdfft?md5=dff34a6b28f71c99775be69cea01f519&pid=1-s2.0-S1319562X24000858-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial communities in petroleum refinery effluents and their complex functions","authors":"Hayfa Habes Almutairi","doi":"10.1016/j.sjbs.2024.104008","DOIUrl":"https://doi.org/10.1016/j.sjbs.2024.104008","url":null,"abstract":"<div><p>Petroleum refinery effluents (PRE) are a significant cause of pollution. It contains toxic compounds such as total petroleum hydrocarbons (TPH), and polycyclic aromatic hydrocarbons (PAHs), as well as heavy metals. They show a huge threat facing the aquaculture habitats, human health, and the environment if they are not treated before discharging into the environment. Physical and chemical procedures are used to treat hydrocarbon pollution in PRE, but these techniques often result in the formation of hazardous by-products during the remediation process. However, PRE contains various microbial communities, including bacteria, yeast, microalgae, and fungi. The bioremediation and biodegradation of oil contaminants are the primary functions of these microbial communities. However, these microorganisms can perform various additional functions including but not limited to heavy metals removal, production of biosurfactants, and nitrogen fixation. This review contributes to the comprehension of natural microbial communities and their complex functions in petroleum refinery effluents. Understanding microbial communities would facilitate the advancement of innovative biotechnology aimed at treating PRE, improving bioremediation processes, and potentially transforming PRE into valuable bio-products. Moreover, it assists in determining the most effective bioaugmentation strategy to enhance biodegradation and bioremediation in PRE. The review highlights the potential for sustainable green approaches using microbial communities to replace toxic chemical therapies and expensive physical treatments in the future.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104008"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X2400086X/pdfft?md5=b2334b7f56f9cc710321a9c1175d8abd&pid=1-s2.0-S1319562X2400086X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140880577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the interplay between microRNA expression and DNA mutation analysis in AML patients","authors":"Rastee H. Saeed ,&nbsp;Zirak Faqe Ahmed Abdulrahman ,&nbsp;Dara K. Mohammad","doi":"10.1016/j.sjbs.2024.104027","DOIUrl":"https://doi.org/10.1016/j.sjbs.2024.104027","url":null,"abstract":"<div><p>MicroRNAs (miRNAs) are key regulators in Acute Myeloid Leukemia AML, affecting gene expression, including that of CD markers and impacting mutations within leukemic cells. Mutations in AML can alter miRNA profiles, which can affect the expression of CD markers and contribute to disease progression by influencing cellular processes such as differentiation, proliferation, and apoptosis. Here, we examined the interplay of cell surface protein expression (CD markers), DNA mutations, and microRNA expression in AML patients. We included 32 recently diagnosed AML patients, and CD marker expression was evaluated using flow cytometry and molecular techniques. This study aims to delve into this relationship within the context of AML, elucidating its potential implications for diagnosis, prognosis, and therapeutic interventions. Mutations were scrutinized in six patients using Whole-Exome Sequencing (WES), while quantitative PCR (qPCR) was employed to investigate the expression levels of nine microRNAs. Subsequently, a comprehensive interaction network was constructed using Cytoscape software, focusing on genes with significant mutations and their corresponding microRNAs. Cell surface protein expression analysis revealed upregulation of CD45, CD99, CD34, HLA-DR, CD38, CD13, CD33, MPO, CD15 and CD117 in AML patients. The molecular analysis results unveiled mutations in specific genes (FLT3, KIT, PTPN11, BCR, DNMT3A, and NRAS) targeted by nine microRNAs. Notably, eight microRNAs exhibited heightened expression levels. Network analysis highlighted interactions between the PTPN11 gene and six scrutinized microRNAs. Understanding the regulatory dynamics between gene mutations and microRNAs in AML patients is pivotal for unraveling the disease’s molecular mechanisms and identifying potential therapeutic targets. Further exploration into the functional roles of microRNAs in gene regulation and AML pathogenesis is warranted to validate their potential as therapeutic targets, diagnostic markers, and advanced treatment strategies.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104027"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001050/pdfft?md5=d262753baa483562925acbbb910877e4&pid=1-s2.0-S1319562X24001050-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141083975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IC - Editorial Board","authors":"","doi":"10.1016/S1319-562X(24)00115-3","DOIUrl":"https://doi.org/10.1016/S1319-562X(24)00115-3","url":null,"abstract":"","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104037"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001153/pdfft?md5=897303caada034ca3ce1a2c262ff9b9e&pid=1-s2.0-S1319562X24001153-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141241575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation effects of three Aspergillus species on iron-based oxides (Hematite − Goethite) in paint layer in oil paintings","authors":"Abatable Thanaa ,&nbsp;Mari Sumayli ,&nbsp;A. El-Shabasy","doi":"10.1016/j.sjbs.2024.104004","DOIUrl":"10.1016/j.sjbs.2024.104004","url":null,"abstract":"<div><p>The inorganic colour layer based on iron oxide is affected by microorganisms (fungi) and leads to its deterioration due to feeding on the mineral elements through the chemical composition of the colour in the presence of a suitable environment (medium). Damage occurs as a result of heavy metal elements being removed from the colour, leading to a defect in the chemical composition and the fading of the colours. The current study showed the effect of the different types of the most common fungi on oil paintings (<em>Aspergillus flavius, Aspergillus fumigatus</em> and <em>Aspergillus niger</em>) after cultivating the different types of fungi and obtaining pure colonies for each fungus separately and conducting a fungal infection on experimental samples with preparing the old techniques, coloured with hematite red and goethite yellow. Each colour is mixed with different proportions of linseed oil (1, 2, 3). They were aged artificially and incubated at a temperature of 26^2+- degrees and examined periodically until the fungi appeared on the surface in the form of colour spots ranging from very dark (severe infestation) to light (low infestation). The change in chemical composition was measured by Raman and EDX analyses of the samples before and after infection. Fungi showed the appearance of spoilage products from metal sulfides and metal carboxylates. The iron oxide ion decreased in both the red and the yellow colours, leading to a change after the fungal infection. Examination of the morphological surface using SEM, USB and measurement of colour change showed the change in the red colour more than the yellow and scattering of green and black colour dots on the surface of the sample. Correlation and Simple Linear Regression were applied for each colour before and after besides both colours together. It was found that these colours appeared around some of the fungal colonies as a result of the activities. Fungal species of some strains reduced Fe⁺³ to Fe⁺² . This provides new insights into the role of microorganisms in the deterioration of painted surfaces.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104004"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24000822/pdfft?md5=5ab8bf9d38e484f2ae7cda947b2ec0ec&pid=1-s2.0-S1319562X24000822-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141041684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical characterization of immobilized recombinant subtilisin and synthesis and functional characterization of recombinant subtilisin capped silver and zinc oxide nanoparticles","authors":"Shreya S. Shettar ,&nbsp;Zabin K. Bagewadi ,&nbsp;T.M. Yunus Khan ,&nbsp;Shaik Mohamed Shamsudeen ,&nbsp;Harsh N. Kolvekar","doi":"10.1016/j.sjbs.2024.104009","DOIUrl":"https://doi.org/10.1016/j.sjbs.2024.104009","url":null,"abstract":"<div><p>This pioneering research explores the transformative potential of recombinant subtilisin, emphasizing its strategic immobilization and nanoparticle synthesis to elevate both stability and therapeutic efficacy. Achieving an impressive 95.25 % immobilization yield with 3 % alginate composed of sodium along with 0.2 M CaCl₂ indicates heightened pH levels and thermal resistance, with optimal action around pH 10 as well as 80 °C temperature. Notably, the Ca-alginate-immobilized subtilisin exhibits exceptional storage longevity and recyclability, affirming its practical viability. Comprehensive analyses of the recombinant subtilisin under diverse conditions underscore its adaptability, reflected in kinetic enhancements with increased V_max (10.7 ± 15 × 10³ U/mg) and decreased K_m (0.19 ± 0.3 mM) values post-immobilization using N-Suc-F-A-A-F-<em>p</em>NA. UV–visible spectroscopy confirms the successful capping of nanoparticles made of Ag and ZnO by recombinant subtilisin, imparting profound antibacterial efficacy against diverse organisms and compelling antioxidant properties. Cytotoxicity was detected against the MCF-7 breast cancer line of cells, exhibiting IC₅₀ concentrations at 8.87 as well as 14.52 µg/mL of AgNP as well as ZnONP, correspondingly, indicating promising anticancer potential. Rigorous characterization, including FTIR, SEM-EDS, TGA and AFM robustly validate the properties of the capped nanoparticles. Beyond therapeutic implications, the investigation explores industrial applications, revealing the versatility of recombinant subtilisin in dehairing, blood clot dissolution, biosurfactant activity, and blood stain removal. In summary, this research unfolds the exceptional promise of recombinant subtilisin and its nanoparticles, presenting compelling opportunities for diverse therapeutic applications in medicine. These findings contribute substantively to biotechnology and healthcare and stimulate avenues for further innovation and exploration.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104009"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24000871/pdfft?md5=45b4fd5c0a6246f605e8063496a9fe72&pid=1-s2.0-S1319562X24000871-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140880578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of phytase producing bacteria from acidifying Tithonia diversifolia: Potential for ruminant feed development","authors":"Roni Pazla ,&nbsp;Gusri Yanti ,&nbsp;Novirman Jamarun ,&nbsp;Mardiati Zain ,&nbsp;Hera Dwi Triani ,&nbsp;Ezi Masdia Putri ,&nbsp;Anifah Srifani","doi":"10.1016/j.sjbs.2024.104006","DOIUrl":"10.1016/j.sjbs.2024.104006","url":null,"abstract":"<div><p>Phytate content in feed ingredients can negatively impact digestibility and palatability. To address this issue, it is necessary to study microbes capable of breaking down phytate content. This study aimed to isolate and characterize phytase-producing bacteria from decaying materials rich in phytic acid. The research was conducted in several stages. The first stage involved isolating phytase-producing bacteria from the acidification of <em>Tithonia diversifolia</em> using growth media containing Na-phytate. Bacterial isolates that produced clear zones were then tested for their activity and ability to produce several enzymes, specifically phytase, cellulase, and protease. The next step was to test the morphological characteristics of the bacterial isolate. The final stage of bacterial identification consisted of DNA isolation, followed by PCR amplification of the 16S rRNA gene, DNA sequence homology analysis, and construction of a phylogenetic tree. Based on research, three isolates were found to produce clear phytase zones: isolates R5 (20.3 mm), R7 (16.1 mm) and R8 (31.7 mm). All isolates were able to produce the enzymes phytase (5.45–6.54 U/ml), cellulase (2.60–2.92 U/ml), and protease (22.2–23.4 U/ml). Metagenomic testing identified isolate R7 and R8 as <em>Alcaligenes faecalis</em> and isolate R5 as <em>Achromobacter xylosoxidans</em>. The isolation and characterization of phytase-producing bacteria from <em>Tithonia diversifolia</em> acidification resulted in the identification of two promising candidates that can be applied as sources of phytase producers. Phytase-producing bacteria can be utilized to improve digestibility and palatability in animal feed.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 7","pages":"Article 104006"},"PeriodicalIF":4.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24000846/pdfft?md5=9d62d7ad9435fb0c94bccecd81175a2e&pid=1-s2.0-S1319562X24000846-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141054224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}