Russian Agricultural Sciences最新文献

{"title":"Conceptual Model of Digital Nitrogen Management in Agricultural Crops","authors":"V. K. Kalichkin, K. Yu. Maksimovich, D. S. Fedorov, L. V. Garafutdinova","doi":"10.3103/s1068367424700071","DOIUrl":"https://doi.org/10.3103/s1068367424700071","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The research was aimed at developing a conceptual model of digital nitrogen management in crops. Cognitive analysis of the knowledge structure in this subject area and conceptual modeling of digital nitrogen management in agrophytocenoses using an object-oriented approach and Unified Modeling Language (UML) were used. A detailed system of diagrams was created, encompassing class, process, and interaction diagrams. The model is anchored on three abstract objects: class (seven in total), attribute (32), and interclass relationships (18), which distribute the main concepts, emphasizing the complexity and multifaceted approaches to digital nitrogen management. The central class is “Agrophytocenosis”, which directly interacts with five classes and indirectly with one class. Attributes are integral to the classes and reflect their specific characteristics. To depict the interaction between the classes and their attributes, four types of relationships are employed in the model: “dependency,” “association,” “aggregation,” and “inner class.” In the process diagram of the digital nitrogen management system, two primary subsystems are highlighted: the analysis and planning subsystem and the adjustment subsystem, as well as tools and sources for data acquisition and processing. A distinctive feature of the developed conceptual model is the application of the temporality principle, integrating static and dynamic processes in the digital nitrogen management system within the agrophytocenosis. Subsystems for analysis, planning, and adjustment of emerging conditions in the management areas allow ensuring more efficient use of nitrogen fertilizers in crops. The conceptual model is aimed at developing a hardware-software complex for diagnostics of nitrogen nutrition of cultivated plants and management of fertilizer application based on modern digital monitoring and data-processing tools.</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"222 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141513478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Soil Acidity and Fertility Status under Different Land Uses Types in Sayo District of Western Ethiopia","authors":"Abu Regasa, Wassie Haile, Girma Abera","doi":"10.3103/s1068367424700137","DOIUrl":"https://doi.org/10.3103/s1068367424700137","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Assessing the status of surface soil acidity under various land-use types on selected soil physicochemical properties is useful to tackle the expansion of soil acidity and mitigate its side effects in agro-ecosystems for the sustainable management of soil resources. As a result, this study was carried out to evaluate the status of soil acidity under different land use types in the Sayo District of Western Ethiopia. A total of 24 composite soil samples were collected from natural forest, cultivated land, grazing land and eucalyptus tree plantation landfrom the surface (0–20 cm) with three replications. Analysis of variance was used to test the mean differences in soil physicochemical properties under different land use types. Accordingly, sand and clay mean values were highest in cultivated and grazing land, respectively, whereas the lowest values were registered under grazing and cultivated land respectively. The lowest (0.93 cm^–3) mean values of bulk density registered under natural forest land and the highest (1.50 cm^–3) mean values recorded under eucalyptus tree plantation land, conversely the lowest (40.11%) and highest (55.99%) mean values of total porosity observed under eucalyptus tree plantation and natural forest land respectively. In the case of soil chemical properties, the highest (3.07) and lowest (0.47) mean values of the exchangeable acidity were observed under cultivated and grazing land respectively and the highest (35.10%) and lowest (3.26%) mean values of AS persentage were recorded under cultivated and grazing land respectively. The lowest (4.99) and highest (5.62) mean soil pH were observed under cultivated and grazing land respectively whereas the lowest (1.81%) and highest (7.94%) mean values of OM registered under cultivated and natural forest land respectively. the lowest (0.12%) and highest (0.47%) mean values of total N observed under eucalyptus tree plantation and natural forest land respectively while the lowest (3.35 mg/kg) and highest (11.95 mg/kg) mean value of available P registered under cultivated and grazing land respectively. Moreover the higher mean values of exchangeable bases, CEC and PBS were recorded under grazing and natural forest land whereas the lower recorded under cultivated and eucalyptus tree plantation land. Conversely, the higher mean values of extracted micronutrients were registered under cultivated and eucalyptus tree plantation land while the lower recorded were under grazing and natural forest land. Generally, the conversion of land use from natural states exaggerates the surface soil acidity of the study sites. Particularly, the cultivation of deforested land and grasslands reduces the soil pH which results in high total exchangeable acidity and acid saturation. The status of essential plant nutrients such as total nitrogen, available P, exchangeable cations and SOM (source of most nutrients) follows the trends of s","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141513476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Powdery Mildew is a Dangerous Disease of Spring Triticale in the Far East","authors":"T. A. Aseeva, K. V. Zenkina","doi":"10.3103/s1068367424700046","DOIUrl":"https://doi.org/10.3103/s1068367424700046","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abatract</h3><p>The studies were carried out in 2015–2022 with the goal of determining a degree of powdery mildew-induced damage to collection samples of spring triticale in the soil and climatic conditions of the Khabarovsk krai of the Far East. Eighty four samples of various ecological and geographical origins were studied. The weather conditions during the observation period were contrasting; at a norm of precipitation for April to August of 466 mm, an excess reaching 16 to 263 mm. Waterlogging contributed to an increase to 100% in the relative air humidity. The experimental site had a heavy loamy meadow-brown podzolized-gley soil. The distribution (<i>R</i>) of powdery mildew in triticale crops was high (80–100%), and the annual intensity of development (<i>P</i>) reached epiphytotic values. Collection varieties of spring triticale were assigned according to the intensity of the disease development: moderately susceptible (13 samples), susceptible (35 samples), and highly susceptible (36 samples). High correlation coefficients (<i>r</i> = 0.861–0.897) were found between the amount of precipitation in the booting-earing phase and the degree of plant powdery mildew-induced infection. A compiled linear regression equation demonstrated a dependence of the intensity of development of the powdery mildew pathogen and the amount of precipitation in the second ten days of June. The following spring triticale varieties had moderate susceptibility to powdery mildew pathogens (<i>P</i> = 30–40%): Amore, Saur, Prag 409, Dagvo (Russia), Lana, Lotos (Belarus), Zgurivskiy, Oberig Kharkovskiy (Ukraine), 70 HN 458 (Canada), Je 57 (USA), Anoas 5, MX 51 (Mexico), and Tleridal (Switzerland).</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141513472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI Solutions for Digital Diagnostics of Grain Crop Diseases (Based on the Example of Pyrenophora teres in Winter Barley)","authors":"I. V. Arinichev, I. V. Arinicheva, G. V. Volkova, Y. V. Yakhnik","doi":"10.3103/s1068367424700034","DOIUrl":"https://doi.org/10.3103/s1068367424700034","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The purpose of this research is to justify the feasibility of using digital intelligent technologies in forecasting the development of net blotch in winter barley. The developed AI solution is a binary decision tree that can predict scenarios of net blotch development: depressive, moderate, and epiphytotic development. To configure the algorithm parameters, we carried out field and laboratory experiments at the Federal Scientific Center for Biological Plant Protection from 2021 to 2023. The preparation of data involved several stages, including setting up of field plots to create an artificial infection background as well as the preparation of an inoculum, sowing of highly susceptible and resistant winter barley varieties, and artificial inoculation. The selected input factors included the observed degree of leaf damage, type of variety resistance, vegetation phase at the time of primary infection, and average relative air humidity during the vegetation phase of infection. The total sample size was 144 observations. The trained model has demonstrated a high classification accuracy on both the training and test datasets at an accuracy rate of more than 96%. Based on the statistical estimate of the significance of the factors influencing the development of net blotch in barley, it is shown that the most influential factor is the current degree of leaf infection (74.3%), followed by the average relative air humidity (11.9%), the resistance of the variety to the disease (10.4%), and the development stage during which infection occurred (3.4%). The proposed solution has a significant practical importance since it provides new opportunities for the diagnostic process of net blotch in winter barley, including high diagnostic rate, accuracy in forecast predictions, and applicability in field conditions.</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"116 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141513479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the Process of Dosing Ultrafine Particles during Vibration Electromechanical Mixing","authors":"V. A. Shakhov, V. A. Pushko, I. G. Boyko, V. V. Gerasimenko, P. G. Uchkin, I. V. Popov","doi":"10.3103/s1068367424010130","DOIUrl":"https://doi.org/10.3103/s1068367424010130","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>A promising direction for improving the balance of the diet of livestock and poultry by trace element composition is the enrichment with ultrafine particles of scarce trace elements. At the same time, it is important that they are evenly distributed throughout the volume of the feed mixture. The purpose of this study is to increase the uniformity of the distribution of ultrafine particles in feed mixture by developing special equipment and improving the technological process. The use of new technologies for preparing feed mixtures based on the control of process parameters makes it possible to obtain a product with specified functional parameters and preserved nutritional properties. Analysis of theoretical studies of the process of dosing and mixing ultrafine particles has shown that the best variant of their motion in the mixture flow is laminar motion. The designed processing line provides for the separate supply of premixed feed mixture and ultraparticles to the vibrating mixer. The efficiency of the mixing process in a vibrating mixer was assessed using three sections of a feed mixture with ultraparticles of iron, zinc, cobalt, and molybdenum powders. The optimal working parameters are achieved under the following conditions: the angular velocity of the vibrating mixer is 3–7 rad/s, the oscillation frequency is 9–37 Hz, the vibration mixing time is 113–333 s, and the time for opening the controlled dispenser (feeding ultrafine particles into the vibrating mixer) is 5–15 s. When using a controlled ultraparticle dispenser and a spherical impeller, the particles were distributed more evenly in the feed. The lowest effect on the value of this indicator in the three sections of the feed mixture has been recorded for zinc (up to 2.7%), followed by iron powders (up to 3.6%) and molybdenum powders (up to 9.5%). The developed equipment had the greatest effect on the uniformity of cobalt distribution (up to 20.5%). At the same time, the deviation from the norm (0.5 mg/kg) did not exceed 2%; i.e., the value of the qualitative indicator of uniformity of the distribution was 98%.</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of Variegated Alfalfa for Creating Intensive Varieties with High Fodder Productivity: Results and Prospects","authors":"V. M. Kosolapov, E. V. Dumacheva, L. D. Sajfutdinova","doi":"10.3103/s1068367424010075","DOIUrl":"https://doi.org/10.3103/s1068367424010075","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Research was carried out in Belgorod oblast in 2019–2023 to assess the dry matter (DM) yield of selection samples of variegated alfalfa, which were obtained by repeated selection of biotypes with high fertility in the year of sowing (“juvenile samples” (JS-samples)) in comparison with the initial breeding varieties to select the best ones. The research was carried out in three randomly arranged plots in five replications. The soil is typical chernozem with a humus content (according to Tyurin) of 4.8%, pH_salt of 6.6, and P₂O₅ and K₂O content (according to Chirikov) of 122 and 180 mg/kg, respectively. The plots were double rowed with a length of 3.5 m and a row spacing width of 30 cm. Multiple selection from alfalfa varieties (Belgorodskaya 86, Krasnoyaruzhskaya 1, and Krasnoyaruzhskaya 2) has made it possible to create JS-samples (B-86, K-1, and K-2), which differ from the initial varieties in a rapid development in the first years of life. JS-samples surpassed the initial varieties in terms of DM yield by 32.1 to 36.0% (<i>p</i> &lt; 0.05) in the year of sowing (mean for 2019–2021) and by 16.9–22.5% (<i>p</i> &lt; 0.05) in the second year of life (mean for 2020–2022). The highest DM yield was characteristic of JS-sample K-2: 490–553 g/m² in the year of sowing (2019–2021) and 799–939 g/m² in the second year of life (2020–2022). At the same time, JS-samples sharply reduced their DM yield in the fourth year of life (mean for 2022–2023). Thus, it was 56.5% of the Belgorodskaya 86 variety for JS-sample B-86, 56.1% of the Krasnoyaruzhskaya 1 variety for JS-sample K-1, and 54.5% of the Krasnoyaruzhskaya 2 variety for JS-sample K-2. The studied JS-samples are promising for creating intensive-type varieties using grass stands of not more than 3 years of life.</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methodology of Real-time Monitoring of the Crop Status Based on Internet of Things Technologies","authors":"I. Yu. Savin, Yu. I. Blokhin, A. V. Chinilin","doi":"10.3103/s1068367424010117","DOIUrl":"https://doi.org/10.3103/s1068367424010117","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Digital technologies are being actively introduced into Russian agriculture at different levels of information analysis (from the plot to the field, farm, region, and whole country). One of the most important values in crop production at the field level is the introduction of systems for accurate, real-time, and automated monitoring of the crop status, the success of which largely predetermines the effectiveness of precision farming systems. The purpose of this research is to develop a methodology for using Internet of Things (IoT) technologies for noncontact monitoring of crops and related meteorological and soil-hydrological parameters. The basis for monitoring is a wireless network that includes sensor nodes equipped with sensors for meteorological parameters and soil moisture and cameras equipped with a fish-eye lens. Sensor nodes equipped with sensors and cameras are placed in the field according to a specially designed scheme individualized for each field. The development of the scheme of sensor placement in the field is based on analysis of long-term archives of satellite data with high spatial resolution and refined soil maps of large scale. Information from sensors is wirelessly transmitted to the network coordinator (or base station) and then to the remote server in the database, where it is automatically analyzed and interpolated to the entire field. The results of the analysis are used to form recommendations for correcting the agrotechnology of crop cultivation. The elements of the methodology have been tested on a number of test fields and have shown a high efficiency. The implementation of the proposed approaches can serve as an alternative to using remote sensing data for crop monitoring in offline precision farming systems.</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amino-Acid Composition of Proteins in Hulled and Naked Oats","authors":"E. N. Shabolkina, S. N. Shevchenko, N. V. Anisimkina","doi":"10.3103/s1068367424010129","DOIUrl":"https://doi.org/10.3103/s1068367424010129","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The purpose of this study was to evaluate the balance of essential amino acids in proteins of hulled and naked oat varieties relative to the “reference” protein (defined on the basis of the FAO/WHO amino acid score) and find out whether it is possible to increase the proportion of essential amino acids in food products and compound animal feeds containing oats. The study was conducted in 2018–2022 in Samara oblast. The Konkur hulled oat variety and the Bekas and Baget naked oat varieties were examined. The highest content of essential amino acids was recorded in naked oat varieties: 39.9–41.0 g/kg. The total content of critical amino acids (lysine, methionine, and tryptophan) in naked oat varieties is 9.4–9.6 g/kg, which is 1.1–1.3 g/kg higher compared to the Konkur hulled variety. The content of essential amino acids per 1 g of protein in the Konkur variety is 249.7, 268.4 mg in the Bekas variety, and 262.9 mg in the Baget variety (69.4, 74.6, and 73.0% of the FAO/WHO recommended standards, respectively). The excess of some essential amino acids in naked oat varieties (whose amino acid scores exceed 100%) disrupts the balance between essential amino acids compared to the “reference” values, thus, increasing the amino-acid score dissimilarity factor (AASDF). It reaches 24.2% in the Konkur variety, 33.9% in the Bekas variety, and 35.5% in the Baget variety; as a result, the biological value of proteins in the Bekas and Baget naked varieties is lower compared to the Konkur hulled variety. The high content of essential amino acids in proteins of naked oat varieties makes it possible to use their grains as a balancing ingredient in food products and forages of high nutritional value.</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"103 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological Plasticity, Yield, and Grain Quality of Different Soybean Varieties under Conditions of Kursk Oblast","authors":"D. V. Dubovik, E. V. Dubovik, A. V. Shumakov, S. I. Krivosheev","doi":"10.3103/s1068367424010051","DOIUrl":"https://doi.org/10.3103/s1068367424010051","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The purpose of the research was to select the most promising soybean varieties for the conditions of Kursk oblast on the basis of the results of the assessment of their environmental stability and plasticity, the yield, and grain quality. The research was performed in a field experiment on typical heavy-loamy chernozem (Kursk oblast, Kursk raion) in 2020–2022. The authors studied 26 soybean varieties from five breeding centers. The HTC in the growing seasons of soybeans was 0.78 in 2020, 1.20 in 2021, and 1.33 in 2022. The ecological plasticity was the greatest (<i>b</i>_<i>i</i>=2.01–2.91) for the varieties Farta, Kioto, Avanta, Sparta, Kofu, Zusha, Shatilovskaya 17, and Kassidi with the yield of 2.17–2.64 t/ha and the lowest for the variety Bara (<i>b</i>_<i>i</i> = 0.44) with the yield of 1.90 t/ha. All the studied varieties were characterized by high ecological stability under conditions of Kursk oblast (<span>(S_{i}^{2})</span> = 0.01–0.65), and the varieties Elana and Arleta were the most stable (<span>(S_{i}^{2})</span> = 0.01). The best homeostaticity was typical for the varieties Bara (<i>Hom</i> = 14.29), Opus (<i>Hom</i> = 10.70), Irbis (<i>Hom</i> = 8.58), and Barguzin (<i>Hom</i> = 7.65). According to the parameter of agronomic stability, all the studied varieties were suitable for growing under the conditions of Kursk oblast (<i>As</i> &gt; 70%). The varieties Bara, Opus, Irbis, and Barguzin were characterized by the greatest agronomic stability (<i>As</i> = 98.7–97.1%). The mean yield over the years of research was the highest for Nordika (2.54 t/ha) and the lowest for Kazachka (1.93 t/ha) among early-ripening varieties. Among the middle-early varieties, the grain yield was the highest for the variety Kofu (2.61 t/ha). The yield of the other varieties of this group of ripeness (Zusha, Slaviya, Kioto, Kassidi, and Amadeus) was lower than that of Kofu by 0.19–0.48 t/ha. The highest protein content in grain was recorded for Khana (45.9%) among early varieties and for Amadeus (45.6%) among middle-early varieties. Early varieties Nordika, Khana, Opus, and Osmon’ (the yield was 2.34–2.54 t/ha), middle-early varieties Kioto and Kofu (2.42–2.61 t/ha), and ultraearly ripening variety Bara of early harvesting may be recommended for the conditions of Kursk oblast.</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Correlation between PRM1, STK35, and IFT27 Gene Expression Levels and Holstein Bull Semen Quality Parameters","authors":"O. Yu. Barkova, D. A. Starikova, I. V. Chistyakova","doi":"10.3103/s1068367424010038","DOIUrl":"https://doi.org/10.3103/s1068367424010038","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Surveys were carried out to analyze the correlation between the mRNA expression levels of the PRM1, STK35, and IFT27 genes and the quality parameters of the Holstein bull native and frozen–thawed sperm to search for effective transcriptomic biomarkers in bull semen. Native and frozen–thawed sperm samples collected from seven Holstein bulls were used in the surveys. In order to solve the study project tasks, eight sperm-quality parameters were examined to perform the real-time analysis of the studied gene expression in native and frozen–thawed sperm. Nonparametric statistical methods, probabilistic approaches, and the Spearman’s rank correlation test were used to process the produced data. The higher-level expression of the studied genes was predominantly recorded in the frozen–thawed sperm compared to the native sperm. No significant correlation between the mRNA expression level of protamine gene (PRM1) and the sperm quality parameters was revealed. The mRNA expression level of gene ITF27 significantly positively correlated with the defective cells contained in the frozen–thawed sperm (0.714, <i>p</i> = 0.05) and the dead cells contained in the native sperm (0.714, <i>p</i> = 0.0545). Negative correlations with the concentration of normal cells contained in the frozen–thawed sperm (–0.750, <i>p</i> = 0.038) and the live cell concentration (–0.714, <i>p</i> = 0.0545) in the native sperm were found. The transcript (mRNA) within gene ITF27 negatively correlated (–0.703, <i>р</i> = 0.0545) with the value for acrosome defects in the frozen–thawed sperm. The reactive oxygen species (ROS) concentration significantly correlated (0.786, <i>p</i> = 0.0251) with mRNA of gene ITF27. The STK35 gene transcript (mRNA) was the only one of all the studied mRNAs that had a moderate negative correlation with the value for sperm motility in the native (–0.692, <i>p</i> = 0.052) and frozen–thawed sperm (–0.876, <i>p</i> = 0.035). The outcomes of these studies may be used to create a system of noninvasive transcriptional markers for bull sperm quality parameters.</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}