O. Yu. Barkova, D. A. Starikova, I. V. Chistyakova
{"title":"Analysis of Correlation between PRM1, STK35, and IFT27 Gene Expression Levels and Holstein Bull Semen Quality Parameters","authors":"O. Yu. Barkova, D. A. Starikova, I. V. Chistyakova","doi":"10.3103/s1068367424010038","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Surveys were carried out to analyze the correlation between the mRNA expression levels of the PRM1, STK35, and IFT27 genes and the quality parameters of the Holstein bull native and frozen–thawed sperm to search for effective transcriptomic biomarkers in bull semen. Native and frozen–thawed sperm samples collected from seven Holstein bulls were used in the surveys. In order to solve the study project tasks, eight sperm-quality parameters were examined to perform the real-time analysis of the studied gene expression in native and frozen–thawed sperm. Nonparametric statistical methods, probabilistic approaches, and the Spearman’s rank correlation test were used to process the produced data. The higher-level expression of the studied genes was predominantly recorded in the frozen–thawed sperm compared to the native sperm. No significant correlation between the mRNA expression level of protamine gene (PRM1) and the sperm quality parameters was revealed. The mRNA expression level of gene ITF27 significantly positively correlated with the defective cells contained in the frozen–thawed sperm (0.714, <i>p</i> = 0.05) and the dead cells contained in the native sperm (0.714, <i>p</i> = 0.0545). Negative correlations with the concentration of normal cells contained in the frozen–thawed sperm (–0.750, <i>p</i> = 0.038) and the live cell concentration (–0.714, <i>p</i> = 0.0545) in the native sperm were found. The transcript (mRNA) within gene ITF27 negatively correlated (–0.703, <i>р</i> = 0.0545) with the value for acrosome defects in the frozen–thawed sperm. The reactive oxygen species (ROS) concentration significantly correlated (0.786, <i>p</i> = 0.0251) with mRNA of gene ITF27. The STK35 gene transcript (mRNA) was the only one of all the studied mRNAs that had a moderate negative correlation with the value for sperm motility in the native (–0.692, <i>p</i> = 0.052) and frozen–thawed sperm (–0.876, <i>p</i> = 0.035). The outcomes of these studies may be used to create a system of noninvasive transcriptional markers for bull sperm quality parameters.</p>","PeriodicalId":21531,"journal":{"name":"Russian Agricultural Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Agricultural Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3103/s1068367424010038","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Surveys were carried out to analyze the correlation between the mRNA expression levels of the PRM1, STK35, and IFT27 genes and the quality parameters of the Holstein bull native and frozen–thawed sperm to search for effective transcriptomic biomarkers in bull semen. Native and frozen–thawed sperm samples collected from seven Holstein bulls were used in the surveys. In order to solve the study project tasks, eight sperm-quality parameters were examined to perform the real-time analysis of the studied gene expression in native and frozen–thawed sperm. Nonparametric statistical methods, probabilistic approaches, and the Spearman’s rank correlation test were used to process the produced data. The higher-level expression of the studied genes was predominantly recorded in the frozen–thawed sperm compared to the native sperm. No significant correlation between the mRNA expression level of protamine gene (PRM1) and the sperm quality parameters was revealed. The mRNA expression level of gene ITF27 significantly positively correlated with the defective cells contained in the frozen–thawed sperm (0.714, p = 0.05) and the dead cells contained in the native sperm (0.714, p = 0.0545). Negative correlations with the concentration of normal cells contained in the frozen–thawed sperm (–0.750, p = 0.038) and the live cell concentration (–0.714, p = 0.0545) in the native sperm were found. The transcript (mRNA) within gene ITF27 negatively correlated (–0.703, р = 0.0545) with the value for acrosome defects in the frozen–thawed sperm. The reactive oxygen species (ROS) concentration significantly correlated (0.786, p = 0.0251) with mRNA of gene ITF27. The STK35 gene transcript (mRNA) was the only one of all the studied mRNAs that had a moderate negative correlation with the value for sperm motility in the native (–0.692, p = 0.052) and frozen–thawed sperm (–0.876, p = 0.035). The outcomes of these studies may be used to create a system of noninvasive transcriptional markers for bull sperm quality parameters.
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