Scandinavian Journal of Immunology最新文献_第9页

Slit2 suppresses endotoxin‐induced uveitis by inhibiting the PI3K/Akt/IKK/NF‐κB pathway Slit2通过抑制PI3K/Akt/IKK/NF - κB通路抑制内毒素诱导的葡萄膜炎

4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-28 DOI: 10.1111/sji.13319

Yong Du, Linbin Zhou, Zijun Wen, Lujia Feng, Shaochong Zhang, Ting Zhang

{"title":"Slit2 suppresses <scp>endotoxin‐induced</scp> uveitis by inhibiting the <scp>PI3K</scp>/Akt/<scp>IKK</scp>/<scp>NF‐κB</scp> pathway","authors":"Yong Du, Linbin Zhou, Zijun Wen, Lujia Feng, Shaochong Zhang, Ting Zhang","doi":"10.1111/sji.13319","DOIUrl":"https://doi.org/10.1111/sji.13319","url":null,"abstract":"Abstract Uveitis is a devastating intraocular inflammatory disease. The secreted leucine‐rich repeat protein slit homologue 2 (Slit2) has been found to be an essential regulator of inflammation. This study aimed to analyse the anti‐inflammatory effects and the underlying mechanisms of Slit2 in an endotoxin‐induced uveitis (EIU) rat model. In this study, rats with EIU pretreated recombinant human Slit2 (rhSlit2) or a control vehicle by intravitreal injection. The clinical scores were graded under a slit lamp. The protein concentrations and total number of cells in the aqueous humour (AqH) were examined, and the retinal expression of various inflammatory mediators was detected. The levels of nuclear factor‐kappa B (NF‐κB), phosphorylated NF‐κB, IkappaB‐a (IκB‐a), phosphorylated IκB‐a, IKK, phosphorylated IKK, PI3Kp85, phosphorylated PI3Kp85, Akt and phosphorylated Akt were evaluated by western blotting. Treatment with rhSlit2 dramatically diminished the clinical scores of EIU, with significant decreases in inflammatory cell infiltration, protein concentrations, cellulose‐like exudates, the production of ICAM‐1, MCP‐1, TNF‐α and IL‐6 in the AqH; and adhesion of leucocytes. The PI3K/Akt/IKK/NF‐κB pathway was found to be activated in EIU. However, the pre‐treatment of rhSlit2 significantly inhibited the production of ICAM‐1, MCP‐1, TNF‐α, and IL‐6, and inhibited leucocyte adhesion by modulating the PI3K/Akt/IKK/NF‐κB pathway. In conclusion, the intravitreal injection of rhSlit2 alleviated EIU‐related inflammation in Sprague–Dawley rats by reducing the proinflammatory cytokines and leucocyte adhesion; in particular, rhSlit2 may inhibit LPS‐induced inflammation by inhibiting the activation of PI3K/Akt/IKK/NF‐κB signalling pathway. Therefore, rhSlit2 shows significant potential for effectively alleviating immune inflammatory responses in vivo.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135425677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TREM2 knockout promotes liver cell apoptosis and inflammation in acute liver injury tre2敲除促进急性肝损伤中肝细胞凋亡和炎症

4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-25 DOI: 10.1111/sji.13330

Shihua Chao, Shulin Shan, Fuyong Song

引用次数: 0

Method for high‐plex analysis of immune cells in human skin using the GeoMx system 使用GeoMx系统对人体皮肤免疫细胞进行高复合体分析的方法

4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-19 DOI: 10.1111/sji.13326

Borislav Ignatov, Daniel Sortebech, Thomas Emmanuel, Ekaterina Zhuravleva, Liv Eidsmo

{"title":"Method for high‐plex analysis of immune cells in human skin using the GeoMx system","authors":"Borislav Ignatov, Daniel Sortebech, Thomas Emmanuel, Ekaterina Zhuravleva, Liv Eidsmo","doi":"10.1111/sji.13326","DOIUrl":"https://doi.org/10.1111/sji.13326","url":null,"abstract":"Abstract Specific T cell populations in the skin have been demonstrated as important disease drivers in several dermatoses. Due to the unique skin architecture, these cells are not grouped together in structures but dispersedly spread out throughout the epidermis. Following tissue disruption and isolation, only about 10% of skin T cells are recovered and any in vitro expansion may alter their bona fide phenotype. The Nanostring GeoMx system was developed to address cellular phenotype and protein expression in a tissue spatial context. To do so, regions of interest (ROI) must exceed a certain area threshold (usually 100 μm in diameter) to generate a sufficient signal‐to‐noise ratio. Here, we present an approach that allows for the pooling of numerous smaller ROIs within the skin, enabling T cell and melanocyte phenotyping. Skin samples from healthy individuals and vitiligo patients were analysed using the GeoMx system and several immune profiling panels. A sufficient signal‐to‐noise ratio was achieved by pooling smaller ROIs and analysing them as a single group. While this prevents spatial analysis, this method allows for detailed analysis of cells as a population in the context of their physiological environment, making it possible to investigate in situ phenotype of rare cells in different tissue compartments.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135063698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the tumour immune microenvironment of hepatocellular carcinoma 解读肝细胞癌肿瘤免疫微环境

4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-12 DOI: 10.1111/sji.13327

Sha Liu, Man Jia, Rongyang Dai

引用次数: 0

Revisiting the relationship between complement and ulcerative colitis 补体与溃疡性结肠炎的关系

4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-11 DOI: 10.1111/sji.13329

Yujie Ma, Kaicheng Zhang, Yuanyuan Wu, Xiaoyan Fu, Shujuan Liang, Meiyu Peng, Juntang Guo, Meifang Liu

{"title":"Revisiting the relationship between complement and ulcerative colitis","authors":"Yujie Ma, Kaicheng Zhang, Yuanyuan Wu, Xiaoyan Fu, Shujuan Liang, Meiyu Peng, Juntang Guo, Meifang Liu","doi":"10.1111/sji.13329","DOIUrl":"https://doi.org/10.1111/sji.13329","url":null,"abstract":"Abstract Ulcerative colitis (UC) is an inflammatory bowel disorder (IBD) characterized by relapsing chronic inflammation of the colon that causes continuous mucosal inflammation. The global incidence of UC is steadily increasing. Immune mechanisms are involved in the pathogenesis of UC, of which complement is shown to play a critical role by inducing local chronic inflammatory responses that promote tissue damage. However, the function of various complement components in the development of UC is complex and even paradoxical. Some components (e.g. C1q, CD46, CD55, CD59, and C6) are shown to safeguard the intestinal barrier and reduce intestinal inflammation, while others (e.g. C3, C5, C5a) can exacerbate intestinal damage and accelerate the development of UC. The complement system was originally thought to function primarily in an extracellular mode; however, recent evidence indicates that it can also act intracellularly as the complosome. The current study provides an overview of current studies on complement and its role in the development of UC. While there are few studies that describe how intracellular complement contributes to UC, we discuss potential future directions based on related publications. We also highlight novel methods that target complement for IBD treatment.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135981082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pimecrolimus protects neuron‐like SH‐SY5Y cells against anti‐inflammatory and anti‐oxidant effects of both microglial secretome and hydrogen peroxide 吡美莫司保护神经元样SH - SY5Y细胞免受小胶质分泌组和过氧化氢的抗炎和抗氧化作用

4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-11 DOI: 10.1111/sji.13328

Fatma Gonca Kocanci, Azize Yasemin Goksu Erol, Fatma Yildiz, Hamiyet Eciroglu

{"title":"Pimecrolimus protects <scp>neuron‐like SH‐SY5Y</scp> cells against anti‐inflammatory and anti‐oxidant effects of both microglial secretome and hydrogen peroxide","authors":"Fatma Gonca Kocanci, Azize Yasemin Goksu Erol, Fatma Yildiz, Hamiyet Eciroglu","doi":"10.1111/sji.13328","DOIUrl":"https://doi.org/10.1111/sji.13328","url":null,"abstract":"Abstract Calcineurin inhibitors have been found to exhibit a preventive role against neuroinflammation, which represents a crucial underlying mechanism in neurodegenerative diseases (ND). Additionally, they possess suppressive effects on the activation of apoptotic pathways, which constitute another mechanism underlying such diseases. Given that pimecrolimus, a calcineurin inhibitor, impedes the synthesis of pro‐inflammatory cytokines, such as interleukin (IL)‐2, IL‐4, and IL‐10, and influences apoptotic processes, it is noteworthy to test its potential neuroprotective properties. Thus, the objective of this investigation was to assess the potential protective effects of pimecrolimus against the degenerative consequences of both microglial secretomes and hydrogen peroxide (H 2 O 2 ), an oxidant agent. The survival rates of HMC3 microglia cells, neuron‐like differentiated SH‐SY5Y (d‐SH‐SY5Y) cells, and their co‐culture were determined using the 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide (MTT) method. Furthermore, the levels of pro‐inflammatory cytokines IL‐1β and IL‐6, and anti‐inflammatory cytokine IL‐10 were measured using ELISA kits, besides total antioxidant and oxidant capacities in conditioned media of cells. Additionally, the effect of pimecrolimus on neurite length in these cell groups was evaluated through morphological observations. This study revealed, for the first time, that pimecrolimus exerts preventive effects on neurodegenerative processes by virtue of its anti‐inflammatory and ‐antioxidant activities. It holds promise as a potential treatment option for ND.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135980874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipokines from adipose tissue and common variable immunodeficiency: Is there any association? 来自脂肪组织的脂肪因子和常见的可变免疫缺陷:有什么联系吗?

IF 3.7 4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-01 Epub Date: 2023-06-05 DOI: 10.1111/sji.13257

Luana Amorim Hassun, Maria Luiza Ricarte Ruggeri, Stefany Alvino de Souza, Alice Mory Rossato, Gabriela Souza Chmieleski, Larissa Scarpini de Carvalho, Adriana Gut Lopes Riccetto, Giovanna Rosa Degasperi

{"title":"Adipokines from adipose tissue and common variable immunodeficiency: Is there any association?","authors":"Luana Amorim Hassun, Maria Luiza Ricarte Ruggeri, Stefany Alvino de Souza, Alice Mory Rossato, Gabriela Souza Chmieleski, Larissa Scarpini de Carvalho, Adriana Gut Lopes Riccetto, Giovanna Rosa Degasperi","doi":"10.1111/sji.13257","DOIUrl":"10.1111/sji.13257","url":null,"abstract":"Adiponectin and leptin are adipokines, secreted by white adipose tissue (WAT), which play an important role in energy homeostasis. Some evidence has shown that adipokine-producing adipose cells present in the bone marrow (BM) appear to exert an influence on hematopoiesis and B cell development. Common variable immunodeficiency (CVID) is one of the most common inborn errors of immunity in humans. In CVID, numerical and/or functional defects of B cells and their precursors result in hypogammaglobulinemia, usually Immunoglobulin (Ig) A and IgG. Manifestations of CVID include immunodeficiency, autoimmunity, inflammation and lymphoproliferation, resulting in a wide range of phenotypes. How adipokines interact and influence the pathophysiology of CVID is still unclear. In this review, we seek to summarize the aspects known so far concerning the interface between adipokines, B cells and CVID. More research is needed to fully understand these interactions; this knowledge is a potential avenue for the discovery of useful biomarkers and may provide new therapeutic targets for the treatment of patients with CVID and related diseases.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46138164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endoplasmic reticulum stress in T cell-mediated diseases. 内质网应激在T细胞介导疾病中的作用

IF 3.7 4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-01 Epub Date: 2023-06-27 DOI: 10.1111/sji.13307

Shaodan Chen, Qiulei Wang, Hui Wang, Sheng Xia

引用次数: 0

Combination of STAT3 inhibitor with Herceptin reduced immune checkpoints expression and provoked anti-breast cancer immunity: An in vitro study. STAT3抑制剂与赫赛汀联合降低免疫检查点表达并激发抗乳腺癌免疫:一项体外研究

IF 3.7 4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-01 Epub Date: 2023-05-24 DOI: 10.1111/sji.13300

Amirhossein Jahangiri, Rana Ezzeddini, Nazanin Zounemat Kermani, Fariborz Bahrami, Amir Salek Farrokhi

{"title":"Combination of STAT3 inhibitor with Herceptin reduced immune checkpoints expression and provoked anti-breast cancer immunity: An in vitro study.","authors":"Amirhossein Jahangiri, Rana Ezzeddini, Nazanin Zounemat Kermani, Fariborz Bahrami, Amir Salek Farrokhi","doi":"10.1111/sji.13300","DOIUrl":"10.1111/sji.13300","url":null,"abstract":"Breast cancer (BC) is the most prevalent diagnosed cancer among women. Herceptin blocks the effects of Her-2 and tumour cell growth. Despite many achievements using Herceptin in Her-2+ invasive BC treatment, there are treatment failures and resistances. The signal transducer and activator of transcription 3 (STAT3) is persistently activated in BC and is associated with immune suppression and tumour cell proliferation. We evaluated whether STAT3 inhibition could increase Herceptin impact on in vitro reduction of immune checkpoint inhibitors and polarize T cells to a protective immune response. We treated SK-BR-3 cells with Herceptin and the STAT3-inhibitor (FLLL32) and assessed the apoptosis and expression of apoptosis-related proteins, VEGF, Her-2 and apoptosis targets of STAT3. PBMCs were isolated from healthy donors and co-cultured with SK-BR-3 cells in the presence or absence of Herceptin and FLLL32. PD-L1, CTLA-4, TIM-3 and T-cell intracellular cytokines were then evaluated. Our results demonstrated that STAT3 inhibition and Herceptin increased SK-BR-3 cell apoptosis, significantly. STAT3 inhibition through combination treatment had a more significant effect on regulating PD-1, TIM-3 and CTLA-4 expression on PBMCs. Alternatively, the combination of FLLL32 and Herceptin promoted T helper-1 protective immune response. The combination of FLLL32 and Herceptin suppress the expression of immune checkpoints and provoke the T-helper1 immune response in lymphocytes. Our analysis indicates STAT3 as a promising target that improves Herceptin's role in breast cancer cell apoptosis.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47654617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidence and predictors of infections in patients with advanced non-small cell lung cancer treated with checkpoint inhibitor immunotherapies: A monocentric retrospective cohort study. 检查点抑制剂免疫疗法治疗晚期非小细胞肺癌患者感染的发生率和预测因素:一项单中心回顾性队列研究

IF 3.7 4区医学

Scandinavian Journal of Immunology Pub Date : 2023-09-01 Epub Date: 2023-06-20 DOI: 10.1111/sji.13303

Davide Fiore Bavaro, Lucia Diella, Pamela Pizzutilo, Annamaria Catino, Fabio Signorile, Francesco Pesola, Alessandra Belati, Ilaria Marech, Vito Garrisi, Nino Lamorgese, Francesco Di Gennaro, Annalisa Saracino, Domenico Galetta

{"title":"Incidence and predictors of infections in patients with advanced non-small cell lung cancer treated with checkpoint inhibitor immunotherapies: A monocentric retrospective cohort study.","authors":"Davide Fiore Bavaro, Lucia Diella, Pamela Pizzutilo, Annamaria Catino, Fabio Signorile, Francesco Pesola, Alessandra Belati, Ilaria Marech, Vito Garrisi, Nino Lamorgese, Francesco Di Gennaro, Annalisa Saracino, Domenico Galetta","doi":"10.1111/sji.13303","DOIUrl":"10.1111/sji.13303","url":null,"abstract":"Immune checkpoint inhibitors (ICIs) represent the cornerstone of the current treatment of non-small cell lung cancer (NSCLC). However, the occurrence of concomitant infections might hamper success. All consecutive patients with advanced NSCLC who started ICIs as a first- or second-line therapy from January 1, 2017 to June 30, 2020 were retrospectively evaluated. The occurrence of infectious events during ICIs was correlated with clinical characteristics, including previous Cytotoxic Chemotherapy (CC), occurrence of immune-related-adverse-events (irAEs). A total of 211 patients were included, 46 (22%) females, with a median (q1-q3) age of 69 (62-76) years. Overall, 85 patients (40%) received ICIs as a first treatment line and 126 (60%) as a second line; 40 patients (19%) had at least one infection during ICIs, and 17 (8%) more than one. Notably, autoimmune diseases (P < .005), neutropenia (P = .001) or infections during previous CC (P = .001), irAEs (P = .006), or steroid therapy for irAEs (P < .001) were associated with infection development. By multivariate Cox-regression, autoimmune diseases (aHR = 6.27; 95%CI = 2.38-16.48; P < .001) and steroid therapy for irAEs (aHR = 2.65; 95%CI = 1.27-5.52; P < .009) were associated with a higher risk of infection during ICIs. Interestingly, autoimmune diseases were confirmed as risk factors in patients treated with ICIs as a first line, while previous infections were the only independent predictor of infections in patients treated with ICIs as a second line. Patients with NSCLC treated with ICIs with concurrent autoimmune disease, receiving steroid therapy for management of irAEs, or having a history of previous infections during CC should be actively monitored for the risk of developing infectious complications.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48615397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0