Russian Journal of Plant Physiology最新文献

{"title":"Insights into the Metabolism of Rice Leaves (Oryza sativa L.) under Shade Stress by Investigating the Metabolite Profile Using Gas Chromatography-Mass Spectrometry (GC-MS) Analysis","authors":"N. P. Tho, N. T. T. Uyen, N. T. P. Thao, N. H. Thanh, D. C. Thien, P. M. Nhut, N. T. Tho","doi":"10.1134/s102144372460630x","DOIUrl":"https://doi.org/10.1134/s102144372460630x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Plant metabolites possess noteworthy biological activities, and their biosynthesis can be influenced by fluctuating light conditions. To elucidate the underlying molecular mechanisms and signaling pathways in rice plants subjected to shade stress, the study investigated the impact of shade on total polyphenols, total chlorophyll, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and metabolite composition in rice leaves using gas chromatography-mass spectrometry (GC-MS). Our results revealed a substantial impact of shading on rice plants as there were reductions in both polyphenol and chlorophyll contents. Additionally, the DPPH radical scavenging activity was found to be diminished under shade conditions. Through GC-MS profiling of rice leaves, it was observed that under no-shade conditions, the plant exhibited an accumulation of abundant monoterpenes, phytosterols, and isoprenoids. Noteworthy compounds included bicyclo[3.1.1]heptane, 2,6,6-trimethyl-, [1S-(1).alpha), 2 isomer (30.89%), 3-Octene, (E)- (22.58%), phytol (8.16%), stigmasterol (7.98%), 22,23-dihydro-stigmasterol (6.06%), 5-Cholesten-3-ol, 24-methyl- (5.22%), and vitamin E (4.67%). In contrast, under shade conditions, rice leaves exhibited an accumulation of fatty acid esters, with notable compounds including 3-Octene, (Z)- (29.14%), ethyl ester of hexadecanoic acid (14.52%), ethyl ester of dodecanoic acid (8.33%), ethyl ester of octadecanoic acid (8.07%), squalene (6.63%), and methyl ester of 9-Octadecenoic (Z)- (6.23%). By examining the composition of metabolites, our findings unraveled the underlying molecular processes and signaling pathways involved in rice leaf metabolism under conditions of reduced light exposure and may have implications for further research on the potential health benefits and utilization of these compounds in diverse applications.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"112 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological Identification of Physiological Changes in Vascular Cell Morphology in the Lower Stem of Winter Barley (Hordeum vulgare L.) during Freezing","authors":"G. Mishra","doi":"10.1134/s1021443724605019","DOIUrl":"https://doi.org/10.1134/s1021443724605019","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The physiological changes caused by freezing stress in the vascular cells of the lower stem of winter barley (<i>Hordeum vulgare</i> L.) were identified using standard histology and microscopy. Zones 1 and 2 denoted the bottom and middle regions, respectively, and zones 3 and 4 the apical regions of the stem. The four zones of the lower stem where freezing injuries occurred were categorized as void formation, tissue separation due to ice formation, and partially or fully clogged vessels with darkly stained unknown substances. In zone 1, an uneven, semi-circular region was identified. Within the innermost leaves in the apical areas, there was evidence of partial and total tissue necrosis. In the apical parts of the mesophyll, ice formation and frost triggered cell plasmolysis. In every zone, three different kinds of vascular cell wall ruptures were noted. Zones 3 and 4 defined various forms of vessel cell wall bursts. In all zones, bordered pits manifested as narrower cell walls inside xylem vessels, perhaps impeding the spread of ice within the vessels. According to this study, freezing might have started at the basal region and moved upward to the lower stem’s apical region. The findings imply that physiological changes in cereal crops recovering from freezing can be studied using conventional microscopy.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"15 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Composition and Ecophysiological Characteristics of the Diatom Nitzschia sp. at Different Stages of the Life Cycle","authors":"S. L. Polyakova, S. N. Zheleznova, N. A. Davidovich","doi":"10.1134/s1021443724604762","DOIUrl":"https://doi.org/10.1134/s1021443724604762","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The work analyzes the biochemical composition and the influence of some abiotic factors on cells of <i>Nitzschia</i> sp<i>.</i> strains at different stages of the life cycle. The results of the experiment showed the ability of cells to grow in the salinity range of 10–60‰ at the pre-reproductive stage and in the range of 4–60‰ at the reproductive stage which indicates a relatively wide salinity tolerance of the studied species. The optimal salinity (25‰) and illumination (2.95 klx) for a 12-h photoperiod were found to be the same for both parent and daughter cells. The light saturation coefficient for them was 1.4 klx, indicating high energy efficiency of photosynthesis. Analysis of the biochemical composition of two <i>Nitzschia</i> sp. strains in the reproductive and pre-reproductive stages of the life cycle was carried out. During the accumulative cultivation a high content of monoenoic and polyenoic acids was observed. In general, the amount of saturated fatty acids (UFAs) was higher in parental cells, and the amount of monounsaturated fatty acids (MUFAs) was higher in post-initial cells. The quantity of polyunsaturated fatty acids (PUFAs) was almost the same in both cultures. The ω-6/ω-3 ratio showed a predominance of ω-3 fatty acids in both parental and daughter strains. <i>Nitzschia</i> sp. represents an interest for biotechnology given its biochemical composition and fairly high energy efficiency when grown in culture. However, it is extremely important to take into account the phase of the life cycle of the strains in order to obtain individual valuable products.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"12 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipids, Lipophilic Components of Halophytes of the Flora of Uzbekistan and Their Biological Activity","authors":"R. P. Zakirova, N. K. Khidyrova, S. D. Gusakova","doi":"10.1134/s102144372460689x","DOIUrl":"https://doi.org/10.1134/s102144372460689x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The review highlights the current state of knowledge of the chemical composition of lipids in halophytic plants. The role of lipids and lipophilic substances of halophytes in protecting plants from the effects of stress factors of abiotic and biotic nature is shown. Data on the composition of lipophilic components of halophytic plants growing in the Aral Sea region and other regions of the republic are presented. The rich composition of fatty acids in lipids of seeds and aerial parts of halophytes is shown. The comparative composition of polyprenols of various cotton varieties depending on resistance to stress factors is given. It was revealed that pre-sowing treatment of seeds of cultivated plants with preparations based on the lipid components of halophytic plants increases their adaptive abilities. Based on the data obtained, the prospects of using halophytes as a source of biologically active lipids and lipophilic substances to protect plants from salinity stress are shown.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"16 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3-Нydroxypyridine Derivatives Reduce Mitochondrial Dysfunction in Pea Seedlings under Conditions of Water Deficiency","authors":"I. V. Zhigacheva, N. I. Krikunova, E. M. Mil, Yu. V. Kuznetsov, I. P. Generozova, P. A. Butsanets, A. N. Goloshchapov","doi":"10.1134/s1021443724605500","DOIUrl":"https://doi.org/10.1134/s1021443724605500","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The possibility of using antioxidants-derivatives of 3-hydroxypyridine: N-acetylcysteinate 2-ethyl-6-methyl-3-hydroxypyridine and carnitinate 2-ethyl-6-methyl-3-hydroxypyridine as plant growth regulators investigated on the pea seedlings mitochondria (<i>Pisum sativum</i> L.) Flora 2 cultivar. The influence of water deficiency (WD) and 3-HP on the functional state of mitochondria was studied. Water deficiency activated lipid peroxidation and led to mitochondrial swelling. An increase in LPO intensity was associated with significant changes in the content of C₁₈ fatty acids The content of linoleic and linolenic acids in the mitochondrial membranes of seedlings decreased by 12.5 and 22.3%, respectively. There were also changes in the content of C₂₀ FA: the content of 20:2 ω6 and 20:1 ω9 decreased by almost 1.65 times and 1.5 times, respectively. The swelling of mitochondria, probably, resulted in a partial loss of cytochrome C from the intermembrane space of mitochondria into the cytosol, which led to a decrease in the electron transport rates at the end section of the respiratory chain. The treatment of pea seeds with antioxidants led to the prevention of lipid peroxidation, the prevention changes in the composition of FAs membranes and mitochondrial morphology and also restored the rate of electron transport at the end section of the respiratory chain of these organelles. By preventing lipid peroxidation, the drugs helped maintain the functional state of mitochondria under WD conditions, which probably increased the resistance of pea seedlings to stress.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"6 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Regeneration and Structural and Physiological Modifications in the Foliages of Cadaba trifoliata (Roxb.) Wight & Arn.: An Endemic Tree of the Family Capparaceae","authors":"M. Manokari, M. Faisal, A. A. Alatar, M. S. Shekhawat","doi":"10.1134/s1021443724606293","DOIUrl":"https://doi.org/10.1134/s1021443724606293","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p><i>Cadaba trifoliata</i> (Roxb.) Wight &amp; Arn. (Capparaceae) is an occasional and endemic tree species of the Peninsular India. In this study, an effective in vitro regeneration system is reported for the first time for this tree, and the impact of growth regulators on the micro-morphometric growth modulations was assessed. Node explants were cultivated on Murashige and Skoog (MS) medium containing 3 types of cytokinins viz., <i>N</i>6-benzyladenine (BA), <i>N</i>6-furfuryladenine (kinetin/Kin), and <i>N</i>6-(<i>meta</i>-hydroxybenzyl)adenine (<i>meta</i>-Topolin/<i>m</i>T). The MS medium with <i>m</i>T at 1.0 mg/L was found the most efficient cytokinin for bud break, resulting in a 90.3% organogenic response and producing 8.0 shoots/explants. Conversely, MS medium with 1.5 mg/L BA produced 4.0 shoots, and only 3.3 shoots resulted in 2.0 mg/L Kin. Additionally, a combination of <i>m</i>T (0.5 mg/L) and 0.25 mg/L indole-3-acetic acid (IAA) increased regeneration frequencies and a maximum of 23.0 shoots (5.4 cm length) were amplified, in contrast a combination of BA + IAA and Kin + IAA resulted in a lesser number of shoots comparatively with shoot tip necrosis. Microscopic analysis showed that the leaves developed on the optimal <i>m</i>T and IAA combination were structurally superior with moderately differentiated cuticle, uniform epidermal layers, and increased mesophyll density and vascular elements. The leaves from BA + IAA and Kin + IAA treatments exhibited micro-structural abnormalities. The highest rhizogenic response (95.8%) and a considerable number of roots (5.0 roots with 4.3 cm length) were regenerated from the shoots derived from <i>m</i>T and IAA combination on 1.25 mg/L indole-3-butyric acid (IBA). About 91.7% of the regenerated plantlets survived during <i>ex vitro</i> acclimatization. The study thus highlights the pivotal role of <i>m</i>T in stimulating the in vitro regeneration and alleviation of micro-structural and physiological disorders in cultures of <i>C. trifoliata</i>.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"6 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Energy and Pro-/Antioxidant Metabolism of Heracleum sosnowskyi Manden. Buds during the Winter Dormancy","authors":"S. P. Maslova, M. A. Shelyakin, E. V. Silina, R. V. Malyshev, I. V. Dalke","doi":"10.1134/s1021443724605780","DOIUrl":"https://doi.org/10.1134/s1021443724605780","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Data on changes in energy status, activity of pro-/antioxidant metabolism during overwintering of <i>Heracleum sosnowskyi</i> vegetative buds were obtained. The buds of <i>H. sosnowskyi</i> are not endodormant and their growth is limited by the decrease of the soil temperature to negative values at the end of November. The optimum temperature for energy storage in autumn was found to be in the range of low positive temperatures (2–5°C). The autumn buds were characterized a high capacity for the cytochrome respiratory pathway (CP, <i>V</i>_cyt), which accounted for 78% of the total respiration. During autumn morphogenesis and winter dormancy of buds, the levels of pro-oxidants, which are the content of thiobarbituric acid reactive substances (TBARS) and H₂O₂ content, remained stable. In December, when a stable snow cover and negative soil temperatures were established, the dormant buds showed 2.5 times lower rate of energy storage and activated alternative respiration capacity (AP, <i>V</i>_alt), as indicated by a 2 times lower <i>V</i>_cyt/<i>V</i>_alt ratio. In early spring, compared to winter dormancy, H₂O₂ levels increased 2-fold and antioxidant enzymes activity increased by 27–78% as insolation increased. Spring buds showed an increase in rate of heat production and a decrease in rate of energy storage, which may be due to spring stress caused by increased insolation. It was concluded that in the tissues of <i>H. sosnowskyi</i> buds subjected to exogenous dormancy, an energy balance between dormancy and growth processes is achieved at the level of respiratory capacity and pro-/antioxidant metabolism.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"109 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and Enzymatic Antioxidant Responses of Several Local Pyrus communis L. cv on Different Rootstocks under NaCl Stress","authors":"M. Aydınlı, E. Kaçal, İ. Gür, F. Yildirim, S. Önder, M. Altındal, Y. Karakurt","doi":"10.1134/s1021443724606074","DOIUrl":"https://doi.org/10.1134/s1021443724606074","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Salt stress poses a substantial threat to fruit cultivation in arid and semi-arid regions. An essential aspect of mitigating this challenge involves understanding the adaptive changes in plants under salinity conditions. In this study, salinity stress (0, 20, 40, and 80 mM) induced in Ankara and Deveci pear varieties grafted onto OH×F 97, OH×F 333, and BA 29 rootstocks. Plant height and diameter, stomatal conductance, photosynthetic pigments and K⁺/Na⁺ ratio exhibited a decreasing trend, indicating the impact of salt stress. Conversely, the salt stress index and the activities of antioxidant enzymes including superoxide dismutase, catalase, and ascorbate peroxidase showed an increasing trend, highlighting the plant’s defense mechanisms against oxidative stress. In our comprehensive evaluation, the Deveci × OH×F 97 demonstrated a higher level of tolerance to sodium chloride stress compared to other combinations. Notably, combinations involving the BA 29, particularly Ankara × BA 29, exhibited more pronounced damage under NaCl stress. An intriguing finding was the announced response of enzymatic antioxidants, where despite a higher increase in SOD activity in the Deveci × OH×F 97, CAT and APX activities were generally higher in the Ankara × BA 29. This suggests the effective operation of enzymatic antioxidants in ameliorating oxidative damage in cells exposed to NaCl stress, irrespective of the overall stress tolerance of the plant, within the <i>Pyrus</i>. This study contributes valuable insights into the complex interplay between pear varieties, rootstocks, and NaCl stress, providing a foundation for informed decision-making in orchard management practices to enhance the resilience of pear cultivation in challenging environmental conditions.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"20 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Screening of Valuable Polyphenol Compounds and Essential Oil Profile of Three Rosa damascene Genotypes, as an Important Plant for Medicine and Food Industry","authors":"E. Nourozi, A. Hedayati, R. Tahmasebi, Sh. Shameh, R. Hosseinzadeh","doi":"10.1134/s1021443724606165","DOIUrl":"https://doi.org/10.1134/s1021443724606165","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p><i>Rosa × damascene</i> Mill. plant is one of the most well-known plants in the history of horticulture, which is cultivated in many regions of the world due to its wonderful aroma and variety of cultivars. This research was perpetrated to investigate the morphological and phytochemical differences of three genotypes between <i>R. × damascena</i> cultivated in Urmia. For this purpose, in RCB (randomized complete block) design, three genotypes of <i>R. × damascena</i> (Tabriz, Kashan and Bi-Khar) were cultivated in the research farm of West-Azerbaijan Branch of ACECR. In the third year after planting and when flowering, traits such as fresh and dry weight of flowers, percentages of essential oil, essential oil components and polyphenol compound were studied. Based on results, the highest flower dry weight (21.5 gram per 100-g fresh flower) was obtained in Kashan genotype and the lowest flower dry weight (17.7 gram per 100 gram fresh flower) was obtained in Bi-Khar genotype. The results of essential oil extraction with Clevenger showed that the highest (0.028%) and lowest (0.011%) essential oil percentage were obtained in Kashan and Bi-Khar genotypes, respectively. Analysis by GC-MS identified 88.60%, 94.94% and 91.22% of essential oil components of Kashan, Tabriz and Bi-Khar genotypes, respectively. Also, 6-octen-1-ol, geraniol, nonadecane and eicosane were the great constituents of essential oils of all three genotypes. The present study show that the ovulated genotypes are a good source of polyphenolic compounds and valuable components of the essential oil of the damask rose.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"12 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiochemical and Molecular Response of the Grafted ‘Bidaneh Ghermez’ Grape Cultivar on Native Rootstocks to Identify Tolerant Combination to Drought Stress in Vineyard Conditions","authors":"S. M. M. Mirfatah, M. Rasouli, M. Gholami, A. Mirzakhani","doi":"10.1134/s1021443724605688","DOIUrl":"https://doi.org/10.1134/s1021443724605688","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Drought stress is one of the most important limitations in the production of agricultural products and especially horticulture in different parts of the world and Iran. The climate changes that have happened in the last few years, it is possible to provide sustainable production of grapes in water stress conditions by examining and selecting cultivars that tolerate to drought stress. For this purpose, an experiment from 2018 to 2022 in the form of split plots in the form of randomized complete blocks with 3 replications in garden conditions to investigate the effect of changes in soil water potential on the morphological, physiological and biochemical characteristics of grafted cuttings on cultivar traits Tolerant to sensitive 7 grape (<i>Vitis vinifera</i> L.) cultivars were carried out. The treatments included 7 selected grape varieties (‘Bidaneh Ghermez’, ‘Bidaneh Sefid’, ‘Rashe’, ‘Sahani’, ‘Kare Royeh’, ‘Moulai’, and ‘Chafte’) as the base and red quince cultivar as scion and three levels of drought stress including humidity. Agricultural (control), 75% (moderate stress) and 55% (severe stress) were based on agricultural moisture discharge. The results showed that with more severe water stress, the amount of proline, ABA, activity of catalase and peroxidase, the expression of <i>CBF4</i>, <i>NCED3</i>, and <i>DHN1</i> genes increased, as well as the amount of total chlorophyll were decreased. In this experiment, the highest proline, abscisic acid, catalase, peroxidase, <i>NCED3</i>, <i>CBF4</i> and <i>DHN1</i> gene expression were observed in this experiment under severe stress treatment. In general, ‘Bidaneh Ghermez’ cultivar grafted on the rootstocks of ‘Chafte’, ‘Moulai’ and ‘Kare Royeh’ had a higher potential for tolerance to drought stress than other grafted combinations tested in most of the traits.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"33 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}