Russian Journal of Plant Physiology最新文献

{"title":"Border Cells of the Root Apex: Role in Adaptation Strategies and Root Immunity","authors":"S. A. Pyatina, E. I. Shishatskaya, N. G. Menzyanova","doi":"10.1134/s1021443724607316","DOIUrl":"https://doi.org/10.1134/s1021443724607316","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Border cells (BCs) are a cell population of the root cap that, during the process of differentiation, separates from the surface of the root apex in the form of single cells, small aggregates, or cell sheets and passes into the rhizosphere space. The functional activity of BCs in the rhizosphere is realized through the production of exometabolites. The review discusses the role of BCs and the root extracellular trap formed from their exometabolites in the processes of adaptation of the root system to various abiotic factors and reactions of the root immune system.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"19 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142250189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal Changes in the Photosynthetic Pigment Complex of Sphagnum Mosses: The case of Sphagnum papillosum Lindb.","authors":"A. K. Shtang, T. I. Ponomareva, D. A. Shpanov","doi":"10.1134/s1021443724607304","DOIUrl":"https://doi.org/10.1134/s1021443724607304","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The authors studied the content of photosynthetic pigments and their ratio during the warm period of the year (May–October) and during the period with stable snow cover (April) in sphagnum moss <i>Sphagnum papillosum</i> Lindb. on an oligotrophic bog in Arkhangelsk oblast. The content of photosynthetic pigments decreased from May to July, reaching a minimum, and then increased again by autumn. The content of pigments in moss tissues under the snow was comparable to the July minimum. Based on the results of factor and cluster analyses, it was suggested that the <i>S. papillosum</i> pigment complex in the first half of the growing season is adapted to high insolation and a long photoperiod, which is reflected by such indicators as the chlorophyll <i>a</i>/<i>b</i> ratio and the proportion of chlorophylls in the light-harvesting complex. In addition, during the growing season, the pigment complex apparently adapts to moisture conditions that depend on precipitation, especially during the period of summer decrease in the bog water level, which reflects the Chl/Car ratio.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142250190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Endophytic Bacteria Bacillus velezensis M66 on Transcriptional Activity of the Genes of the RNA Interference System upon Development of Defense Responses Against the Late Blight Pathogen Phytophthora infestans (Mont.) de Bary","authors":"A. V. Sorokan, V. F. Gabdrakhmanova, I. S. Mardanshin, I. V. Maksimov","doi":"10.1134/s1021443724607407","DOIUrl":"https://doi.org/10.1134/s1021443724607407","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The effect of bacteria <i>Bacillus velezensis</i> strain М66 on resistance of potato plants to oomycete <i>Phytophthora infestans</i> (Mont.) de Bary that causes late blight disease was investigated. For the first time, a rise in the number of endophytic bacteria <i>B. velezensis</i> М66 in internal tissues of plants after infection with the pathogen as compared with plants solely inoculated with bacteria was shown. A considerable reduction in the area showing the symptoms of late blight disease was shown irrespective of virulence of the used strain of pathogen. Formation of plant resistance under the effect of <i>B. velezensis</i> М66 cells was accompanied by activation of trypsin inhibitor and peroxidases; accumulation of hydrogen peroxide and transcripts of genes encoding inhibitors of proteinases, β-1,3-glucanase, and anionic peroxidase; and reduction in the level of transcripts of gene <i>PR1</i>: a marker of the development of salicylate-depending reactions. As to the system of RNA interference, it was shown that aggressive strain <i>P. infestans</i> Sn stimulated accumulation of transcripts of the gene encoding Dicer-like protein (DCL) in potato plants, whereas less aggressive strain <i>P. infestans</i> 1840 activated genes <i>DCL</i> and <i>Ago4</i>. Plant treatment with bacteria <i>B. velezensis</i> М66 promoted accumulation of gene <i>Ago1</i> transcripts both in healthy and infected plants. Inoculation of plants with bacteria and subsequent infection with oomycete promoted accumulation of transcripts of all the examined genes involved in the system of RNA interference. Thus, inoculation of plants with cells of endophytic bacteria <i>B. velezensis</i> strain M66 promotes formation of resistance to oomycete <i>P. infestans</i> by means of efficient priming of plant immune potential.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"137 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142250193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytomelatonin As an Element of the Plant Hormonal System","authors":"V. V. Kusnetsov, I. A. Bychkov, N. V. Kudryakova","doi":"10.1134/s1021443724606839","DOIUrl":"https://doi.org/10.1134/s1021443724606839","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Melatonin (N-acetyl-5-methoxytryptamine), an animal hormone, antioxidant, and regulatory molecule, is attracting increasing attention from biologists. Melatonin, discovered in plants in 1995 and later named phytomelatonin (PMT), regulates many stages of plant ontogenesis, from seed germination to the aging process. PMT is one of the most powerful plant cell antioxidants. Numerous experimental data show that PMT increases plant resistance under conditions of both abiotic and biotic stress. In the regulation of physiological processes, it interacts with almost all currently known phytohormones. The biosynthesis of PMT and its multifunctional activity have now been studied quite well; the first receptor and some components of its signaling chain have been discovered. All this allows us to consider PMT a new plant hormone.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"212 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, Physiological, and Biochemical Characteristics of the Sarcinoid Microalga Chlorosarcinopsis eremi (Chlorophyceae, Chlorophyta)","authors":"N. V. Dantsyuk, I. N. Chubchikova, A. D. Temraleeva, G. S. Minyuk, I. V. Drobetskaya","doi":"10.1134/s1021443724607328","DOIUrl":"https://doi.org/10.1134/s1021443724607328","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The strain VKM Al-132 of green sarcinoid microalgae isolated from chestnut soil of the dry steppe zone (Volgograd oblast of Russia) was investigated. With the help of light- and scanning electron microscopy, together with the molecular phylogenetic analysis of the 18S rRNA gene and the ITS2 spacer, the strain was identified as <i>Chlorosarcinopsis</i> <i>eremi</i>. The characteristics of its growth and secondary carotenogenesis were examined under conditions of two-stage batch culture. After 21-day cultivation, the average productivity was 0.12 g/(L day) (dry biomass) and 0.2 mg/(L day) (total carotenoids). At the end of the stage of secondary carotenogenesis, when the percentage of total carotenoids was approximately 0.25% of dry biomass, canthaxanthin and diesters of astaxanthin fractions dominated; the sum of astaxanthin esters attained 36% of total carotenoids. It has been shown that the VKM Al-132 strain of <i>C.</i> <i>eremi</i> may serve as a potentially perspective object to search for optimal conditions for intensification of carotenoid biosynthesis.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"33 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Seed Treatment with Salicylic Acid on Growth, Antioxidant Enzyme Activity, and Proline Content in Wheat Leaves at Excessive Zinc Level in the External Environment","authors":"A. A. Ignatenko, I. A. Nilova, N. M. Kaznina, A. F. Titov","doi":"10.1134/s1021443724607286","DOIUrl":"https://doi.org/10.1134/s1021443724607286","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>We studied the effect of seed treatment with salicylic acid (SA, 100 μM) on seed germination, growth, the level of lipid peroxidation (LPO), and the functioning of a number of components of the antioxidant system in wheat plants (<i>Triticum aestivum</i> L.) Zlata variety grown with optimal (2 μM) or excess (1500 µM) zinc content in the root environment. It has been shown that excess zinc has an inhibitory effect on root and shoot growth and also enhances the accumulation of one of the indicators of LPO development: malondialdehyde (MDA). At the same time, the structural integrity of the membranes was maintained, as indicated by the absence of changes in membrane permeability, recorded by the yield of electrolytes. The activity of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), did not change under these conditions, but the activity of guaiacol-specific peroxidase (GPO) decreased. Treatment of seeds with SA at a concentration of 100 μM, which stimulates their germination, enhanced the negative effect of excess zinc on growth performance and MDA accumulation in wheat leaves. However, in this case, the yield of electrolytes did not increase, which is presumably due to an increase in the activity of SOD and GPO. Based on the data presented in the work and previously obtained, as well as an analysis of the literature, it was concluded that the response of plants to excess zinc in the external environment, like the reaction to other abiotic stresses, is not only multicomponent and multiechelon but also variable, depending on many factors and circumstances: the dose of exposure (zinc concentration and duration of its action), biological characteristics of the plant species (variety), their age condition, etc. Treatment of seeds with SA can change the nature of the plant’s response to the action of excess zinc, and the direction of these changes will, in turn, depend on the concentration of SA, the duration of its action, and the method of treatment.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"5 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142250154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fractional and Fatty Acid Composition of Lipids from the Bud Parts of Plants of the Betula L. Genus by Opening Phases","authors":"I. V. Morozova, N. P. Chernobrovkina, V. P. Pchelkin","doi":"10.1134/s1021443724605937","DOIUrl":"https://doi.org/10.1134/s1021443724605937","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In plants of the genus <i>Betula</i> L. with morphological characteristics of downy birch <i>B. pubescens</i>, silver birch <i>B. pendula</i>, and Karelian birch <i>B. pendula</i> var. <i>carelica</i> in bud fragments, according to the phases of their blossoming, growth and moisture indicators were studied as were the content and composition of fatty acids (FA) of individual fractions of total lipids (TL). Fractions of neutral lipids (NL), glycolipids (GL), and phospholipids (PL) were isolated from TL using chloroform, acetone, and methanol, respectively. Growing rudimentary leaves were distinguished by increased weight and moisture content compared to scales and stipules. In the embryonic leaves, in comparison with other fragments of buds in the phases of blooming, a high absolute content of TL in phase I and a drop in the level of TL in other phases, a significant absolute accumulation of NL and PL by phase III and use in phase IV with the accumulation of GL in this phase, a high level of unsaturated fatty acids (UFA) of TL and their individual fractions during the study period, an increase in the level of linolenic acid (C_18:3) in the composition of PL and GL to phase IV, and an increase in the relative content of palmitic acid (C_16:0) in the composition of GL by phase III and a decrease in its level in phase IV were noted. The fatty acid composition of the TL fractions of fragments of opening birch buds were represented predominantly by UFAs (up to 88% of the total FAs). The main share of UFAs was C_18:3 and linoleic (C_18:2) (up to 55 and 47% of the total FAs, respectively), and C_16:0 predominated (up to 42%) in saturated FAs. In three birches, during the development of buds according to the phases of blossoming in their individual fragments, both in the TL and in all fractions of the TL, the relative content of C_18:2 decreased with an increase in the composition of GL and PL: C_18:3. In these birches, which differ in morphological characteristics, distinctive features of the lipid composition are revealed mainly in the scales.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"19 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142250194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Turnover and Possible Physiological Significance of Purine Crystals in the Cells of the Chlorophytes from the Genus Coelastrella (Scenedesmaceae, Chlorophyta)","authors":"G. A. Kazakov, P. A. Zaitsev, D. A. Chudaev, E. Yu. Parshina, A. V. Moiseenko, A. A. Zaitseva, T. A. Fedorenko, M. G. Bokov, P. Mojzeš, E. S. Lobakova, A. E. Solovchenko","doi":"10.1134/s1021443724606098","DOIUrl":"https://doi.org/10.1134/s1021443724606098","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The productivity of microalgal cultures and their resilience to unfavorable conditions is largely determined by the availability of mineral nutrients, particularly nitrogen. Nitrogen starvation is a strong stressor that induces a broad range of responses in microalgae at the cell and cell population (culture) levels. These responses such as lipogenesis and secondary carotenogenesis are widely used in biotechnology to obtain valuable secondary metabolites of microalgae. It was believed that microalgal cells lack specific structures that function as long-term nitrogen depot, but recent studies assigned this role to microcrystalline inclusions constituted by nitrogenous bases (most often guanine). It is also known that purine microcrystals are biophotonic structures widespread in nature. These structures modify the intensity and spectral composition of radiation in illuminated cells and tissues of living organisms. In this regard, we studied (i) the dynamics of the formation of guanine crystals in the cells of green carotenogenic microalgae from the genus <i>Coelastrella</i> (<i>C. thermophila</i> NAMSU CM1/23 and <i>C. rubescens</i> IPPAS C-2066) depending on the availability of nitrogen in the medium and (ii) the effect of the presence of these crystals on cell resilience to high light intensity. Nitrogen-rich crystal inclusions were accumulated in cells when the cultures reached the stationary growth phase in a medium with ample nitrate nitrogen. During rapid growth, these inclusions were hardly detectable in the cells and disappeared during cultivation in the absence of nitrogen. Optical polarization microscopy and Raman microspectroscopy demonstrated that these nitrogen-rich inclusions are birefringent microcrystals composed of guanine. <i>C. thermophila</i> cells harboring abundant guanine crystals showed resistance to short-term (10–15 min) exposure to high-intensity light (600 µmol PAR quanta/m²/s). The results obtained are discussed in the context of the multifaceted role of guanine crystals in the stress tolerance of microalgae.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"2 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding Phytotoxicity: The Predictive Power of Total Soil Copper Content in Long-Term Pepper Growth in Copper-Polluted Soils","authors":"P. Peñaloza, S. Valdebenito, K. Vidal, M. T. Mukhina, Yu. A. Krutyakov, A. Neaman","doi":"10.1134/s1021443724604853","DOIUrl":"https://doi.org/10.1134/s1021443724604853","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The predictive accuracy of total metal content in contaminated soils for determining phytotoxicity has long been debated. Attempts to determine the “phytoavailable” metal fraction have yielded inconsistent results. Further complications arise in contaminated soils containing multiple metals, making interpretation of results difficult. Therefore, our study focused on an agricultural field in El Melón, Valparaíso region, central Chile, which is predominantly contaminated with copper due to the destruction of a tailings dam by the 1965 earthquake. Our primary objective was to determine which soil copper pool, either soluble or total, controls copper phytotoxicity at this unique site. Total copper ranged from 76 to 1672 mg/kg, while soluble copper (extracted by 0.1 M KNO₃) ranged from 0.11 to 0.34 mg/kg. Using a prolonged 128-day ecotoxicity assessment with pepper (<i>Capsicum annuum</i> L.), our results indicate that total soil copper content emerges as a robust predictor of various plant responses. Regressions showed significant relationships for shoot copper content (<i>R</i>² = 0.77, <i>P</i> &lt; 0.001), shoot dry weight (<i>R</i>² = 0.56, <i>P</i> = 0.02), xylem thickness (<i>R</i>² = 0.33, <i>P</i> = 0.08), and leaf thickness (<i>R</i>² = 0.29, <i>P</i> = 0.10). Conversely, the influence of soluble copper concentration on pepper responses and shoot copper content was not statistically significant (<i>P</i> &gt; 0.1). Our discussion underscores that plant element uptake depends not only on the concentrations in the soil solution (intensity), but also on the total element content in the soil (quantity) and its supply kinetics (capacity). Therefore, the total metal content of the soil was found to be a more reliable predictor of plant responses than the soluble copper fraction in the soil. The anatomical changes observed in this study represent, to the best of our knowledge, the first report of metal-induced stress in <i>Capsicum annuum</i>. From this novel perspective, the results of our study are significant, especially for plant water relations, given their dependence on xylem and leaf thickness.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"83 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Cold Plasma Priming on Certain Traits of Durum Wheat Plants under Salinity Conditions","authors":"R. E. Duran, U. Kilic, U. Kara","doi":"10.1134/s1021443724605287","DOIUrl":"https://doi.org/10.1134/s1021443724605287","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The application of cold plasma as an environmentally friendly, efficient, and cost-effective method has garnered interest for its potential to alleviate the deleterious effects of abiotic stress on plants. This study investigates the impact of nitrogen oxide (NO) cold plasma treatment on wheat (<i>Triticum durum</i> Desf. ‘GAP’) seed germination, seedling growth, and pigment composition under salinity stress conditions. Seeds were exposed to NO cold plasma for 0, 5, 10, and 15 minutes and subsequently sown in Petri dishes with sodium chloride (NaCl) concentrations of 0, 100, 150, and 200 mM to assess morphological and physiological responses between the 7th and 10th days of germination. Results indicated that cold plasma treatment significantly enhanced germination rates and seedling growth under both control and saline conditions, with the 15-min exposure yielding the most pronounced improvements. However, cold plasma treatment alone either decreased leaf pigment content or had no significant effect, whereas under salinity stress, chlorophyll <i>a</i>, chlorophyll <i>b</i>, total chlorophyll, and carotenoid levels showed varied increases with treatment duration. Conversely, anthocyanin levels decreased under salt stress with plasma treatment. The differential effects on pigment composition highlight a complex interaction between cold plasma treatment and plant physiological responses under abiotic stress, suggesting avenues for further research into optimizing treatment protocols for agricultural resilience. This study contributes to the growing body of knowledge on cold plasma applications in agriculture, offering insights into sustainable practices that could mitigate the impacts of global challenges like soil salinity on crop production.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"81 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}