Scandinavian Journal of Clinical & Laboratory Investigation最新文献

{"title":"Impact of preanalytical factors on the stability of acetylcholine receptor antibodies in serum.","authors":"David Lyse Overgaard, Tina Parkner, Josefine Jul Jarbæk Nielsen, Henning Andersen, Cindy Soendersoe Knudsen","doi":"10.1080/00365513.2025.2500011","DOIUrl":"10.1080/00365513.2025.2500011","url":null,"abstract":"<p><p>This study investigated the preanalytical stability of acetylcholine receptor antibodies (AChRAb) in patient samples, a crucial biomarker for diagnosing Myasthenia Gravis (MG). The objective was to evaluate the impact of delayed centrifugation, storage temperature, and repeated freeze-thaw cycles on the accuracy of AChRAb test results. Blood samples were collected from nine MG patients, and four stability studies were conducted. These studies examined: (I) the effect of delayed centrifugation on whole blood (up to 7 h at room temperature), (II) storage of serum at room temperature (up to 7 days), (III) storage of serum at -20 °C (up to 26 weeks), and (IV) the impact on serum of repeated freeze-thaw cycles (up to 3 cycles). AChRAb levels were measured using ELISA, and the results were analysed for statistical variation against baseline measurements using bias limits of ±15%. The findings revealed that AChRAb remained stable for up to 7 hours of delayed centrifugation, 5 days at room temperature, 13 weeks at -20 °C, and after 3 freeze-thaw cycles. However, the antibody levels showed instability after 7 days at room temperature and 26 weeks at -20 °C, where the percentage differences exceeded acceptable limits. Analytical variation, especially at low antibody levels, and differences between ELISA kit lots were potential factors contributing to these findings. In conclusion, AChRAb shows good stability if care is taken to avoid prolonged storage and handling times to maintain diagnostic accuracy.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"308-312"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological findings in Swedish cases of suspected drug-facilitated sexual assault (DFSA).","authors":"Anna Möller, Isabelle Högbeck, Markus R Meyer, Daniela Wissenbach, Olof Beck, Anders Helander","doi":"10.1080/00365513.2025.2511305","DOIUrl":"10.1080/00365513.2025.2511305","url":null,"abstract":"<p><p>Suspicion of proactive drug-facilitated sexual assault (DFSA) where the perpetrator covertly administers psychoactive drugs to the victim has been reported in considerable proportions from sexual assault centers and forensic units all over the world. Substances implicated in DFSA are often referred to as 'date-rape drugs'. This study investigated toxicological findings in cases of suspected proactive DFSA presenting to the Emergency Clinic for Rape Victims in Stockholm, Sweden within 48 h of the assault. Urine samples for toxicological analysis were collected on the first visit to the clinic. During follow-up 10-14 days later, participants provided a control urine sample and reported voluntary intake of alcohol, recreational and prescription drugs in connection with the assault according to a standardized protocol. Urine samples were subjected to extensive toxicological LC-MS/MS analysis that covered common recreational drugs and >100 DFSA-associated substances. 31 out of the 55 women who presented to the clinic after a suspected proactive DFSA during the study period returned for follow-up and completed the study. Almost all women (97%) reported voluntary alcohol intake in connection with the assault, which exceeded 70 g ethanol in half of the cases. Unexpected toxicological findings were made only in five cases (16%), with the most common substance being cocaine. No typical date-rape drug was identified in cases where involuntary intake was considered likely. In conclusion, the greatest risk factor exploited by perpetrators of DFSA appears to be voluntary alcohol intoxication, while toxicological evidence of illicit drugging is rare.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"269-274"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of cystatin C as a new marker of glomerular filtration rate, and of shrunken pore syndrome - a new kidney disorder defining selective glomerular hypofiltration syndromes - calls for expansion of the international KDIGO guidelines.","authors":"Anna Åkesson, Carl Öberg, Linnea Malmgren, Christopher Nilsson, Yoshi Itoh, Søren Blirup-Jensen, Veronica Lindström, Magnus Abrahamson, Felicia Leion, Isleifur Olafsson, Henrik Bjursten, David Grubb, Erik Herou, Alain Dardashti, Johann Sigurjonsson, Liana Xhakollari, Agne Laucyte-Cibulskiene, Hans Pottel, Helena Strevens, Danielle Damm, Magnus Förnvik Jonsson, Joanna Siódmiak, Johan Ärnlöv, Anders Larsson, Torbjörn Åkerfeldt, Kim Kultima, Peter Ridefelt, Johanna Helmersson-Karlqvist, Martin Magnusson, Magnus Hansson, Anna Sjöström, Inga Soveri, Olav Tenstad, Johan Mårtensson, Carl-Gustaf Elinder, Lorenz Risch, Martin Risch, Lars-Olof Hansson, Christopher P Price, Ulf Nyman, Jonas Björk, Pierre Delanaye, Arend Bökenkamp, Anders Christensson, Anders Grubb","doi":"10.1080/00365513.2025.2546320","DOIUrl":"10.1080/00365513.2025.2546320","url":null,"abstract":"<p><p>Cystatin C was identified as a marker of glomerular filtration rate (GFR) in 1979, and the parallel analysis of cystatin C and creatinine led to the identification of shrunken pore syndrome (SPS) - a new kidney disorder - in 2015. Since then, it has been shown that cystatin C in many aspects is superior to creatinine as a marker of GFR and cardiovascular risk. SPS, an entity within the selective glomerular hypofiltration syndromes (SGHS), has been demonstrated to be associated with a strong increase in morbidity and mortality in several populations. Despite the seriousness of SPS and SGHS, and the availability of potential treatments, many patients with these conditions remain undiagnosed, due to the limitations of the international Kidney Disease Improving Global Outcomes Organization (KDIGO) guidelines. Given the significant clinical advantages of cystatin C in diagnosing and treating kidney disorders, there is a need to expand the KDIGO guidelines to include cystatin C measurements alongside creatinine at least in the initial patient evaluation but also in follow-up evaluations. This would improve the early detection and management of patients with kidney diseases, ultimately enhancing patient outcomes. The present discourse summarizes the development of this understanding from the original observations in 1979 and 2015 to the latest findings.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"409-419"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144967109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age- and sex-specific reference values for FIB-4 derived from the Nordic Reference Interval Project (NORIP).","authors":"Lena Hamelius, Gunnar Nordin, Per Bjellerup, Anders Larsson","doi":"10.1080/00365513.2025.2559352","DOIUrl":"10.1080/00365513.2025.2559352","url":null,"abstract":"<p><strong>Introduction: </strong>The Fibrosis-4 (FIB-4) index is a non-invasive biomarker that is widely used to assess liver fibrosis. A limitation is that the enzyme methods (aspartate transaminase (AST) and alanine transaminase (ALT)) are not specified in studies. The aim of the present study was to define reference values for FIB-4 index using Nordic Reference Interval Project data where the results for P-AST and P-ALT are traceable to the IFCC reference measurement procedures.</p><p><strong>Material and methods: </strong>FIB-4 values from 1161 subjects (550 males and 611 females) in the NORIP study were used to calculate reference intervals for the index. The 2.5th and 97.5th percentiles for these markers were calculated according to the International Federation of Clinical Chemistry guidelines.</p><p><strong>Results: </strong>Age (18-<39.9, 40-59.9 and 60-85 years) and sex-specific reference intervals were calculated. Of the components used to calculate the FIB-4 index the age had the greatest impact. In the cohort older than 60 years, 7.5% of the females and 18.2% of the males were above the 2.67 limit for high risk of advanced fibrosis. In the same age group 84.2% of the females and 77.6% of the males had FIB-4 values above the 1.3 limit for intermediate risk and further evaluation required. These are very high proportions which means that a very large proportion of the patients will have false positive results when testing populations with a low disease prevalence.</p><p><strong>Conclusion: </strong>FIB-4 index increases with age, potentially leading to overestimation of fibrosis in elderly patients if age specific decision values are not used.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"462-467"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of measurement uncertainty in direct and estimated serum osmolality according to ISO/TS 20914: implications for clinical diagnostics.","authors":"Mehmet Fatih Alpdemir, Sezen Tutar, Medine Alpdemir","doi":"10.1080/00365513.2025.2487988","DOIUrl":"10.1080/00365513.2025.2487988","url":null,"abstract":"<p><p>This study examines the measurement uncertainties (MU) associated with osmolality (OSM), a crucial parameter in clinical diagnostics and treatment. The research compares the uncertainties of directly measured osmolality (dOSM) with those of estimated osmolality (eOSM), which is calculated based on sodium, glucose, and urea levels. Using ISO/TS 20914 guidelines, the study evaluates the MUs of eOSM across different analyzer models and internal quality control (IQC) lots and compares these values with dOSM. In the materials and methods section, sodium, glucose, and urea analyses were performed using two different analyzers (Atellica CH and Advia Chemistry XPT, Siemens Healthineers). OSM was measured using the freezing point depression method (Osmomat Auto, Gonotec) and data were collected to calculate eOSM. MU (<i>k</i> = 2, 95% confidence) calculations were conducted according to the ISO/TS 20914 standard for each system. The results show that the expanded standard MU (<i>k</i> = 2, 95% confidence) for dOSM is low at 5.56 mOSM/L, while the MU for eOSM is 8.54 mOSM/L for the Atellica CH system and 11.13 mOSM/L for the Advia Chemistry XPT system. These findings indicate that eOSM has higher uncertainty, suggesting it should be used with caution in clinical practice.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"184-189"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preanalytical stability of 17 analytes in whole blood during transportation.","authors":"Ida Marie Nørum Wigh, Jesper Erik Karl Staffansson Strandberg, Marianne Christina Harbo Mosgaard, Maja Boe, Mia Séne Lund Christensen, Simon Lykkeboe, Søren Lundbye-Christensen, Aase Handberg, Stine Krogh Venø","doi":"10.1080/00365513.2025.2524725","DOIUrl":"10.1080/00365513.2025.2524725","url":null,"abstract":"<p><p>Reliable biochemical measurements are essential to patient care and rely on the correct preanalytical handling of blood samples. In Denmark, whole blood samples are transported from general practitioners to local hospital laboratories daily, and whole blood stability for 10 h is required for analytes to be included in this transportation service in the North Jutland Region. Therefore, this study aimed to determine the stability of 17 analytes in whole blood during transportation and storage for 6 and 10 h at room temperature (21 °C). Ten randomly selected participants were included in the Department of Clinical Biochemistry at Aalborg University Hospital. Three serum vacutainer tubes were collected from each participant, and 17 analytes (lipoprotein a, immunoglobulin G1, β-2-Microglobulin, κ free light chain, λ free light chain, peptidyl peptidase A, protein, apolipoprotein B, insulin-like growth factor 1, somatotropin, thyroxin binding globulin, anticardiolipin antibodies IgM, prolactin, thyroid peroxidase antibodies, thyroglobulin, thyroglobulin antibodies,17-Hydroxyprogesterone) were measured at baseline, after 6 h, and after 10 h. All analytes showed 6- and 10-hour stability within the limits of the preestablished analytical variation, reflected by coefficients of variation, in the laboratory (accredited by ISO-15189). Thus, transportation and storage of whole blood at room temperature for 6 and 10 h did not result in significant changes in the levels of the 17 studied analytes. Therefore, all analytes were suitable for the transportation service in the North Jutland Region, Denmark.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"340-345"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability of 17 endocrine analytes in serum or plasma after four cycles of repeated freezing and thawing.","authors":"Elisabet Tjernvoll, Arne Åsberg, Ingrid Alsos Lian, Lena Løfblad, Gunhild Garmo Hov, Ketil Thorstensen, Gustav Mikkelsen","doi":"10.1080/00365513.2025.2487989","DOIUrl":"10.1080/00365513.2025.2487989","url":null,"abstract":"<p><p>Publications of systematic studies on analyte stability after repeated freezing and thawing of samples are rare. We examined the stability of 17 endocrine analytes in pooled serum and/or EDTA plasma after one to four cycles of repeated freezing at -80 °C and thawing. Pooled serum and plasma samples were used. Following baseline measurements in fresh samples (T0), four aliquots (T1-T4) were frozen at -80 °C, and subjected to one to four cycles of freezing and thawing before analysis on the same day. Results were compared to baseline measurements (T0) and to samples frozen once (T1), and were adjusted using quality control material to account for analytical variation between the two time points of analysis. Analytes were considered stable based on statistical significance and percent change compared to allowable bias (AB) based on biological variation. According to criteria based on AB, serum 17-OH progesterone, aldosterone, androstenedione, anti-müllerian hormone, cortisol, dehydroepiandrosterone sulphate, proinsulin C-peptide and sexual-hormone-binding-globulin, and plasma aldosterone and cortisol were stable for four cycles of freezing and thawing. Only serum free thyroxine was considered unstable. For serum erythropoietin, estradiol, free triiodothyronine, human chorionic gonadotropin, human growth hormone, insulin-like growth factor-1, prolactin and plasma free thyroxine, human growth hormone, parathyroid hormone results were not conclusive. Based on average results of pooled samples, eight out of 17 analytes appeared stable for four freeze-thaw cycles compared to AB, while free thyroxine in serum increased more than AB.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":"85 3","pages":"190-195"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of hemolysis as a source of interference in the determination of S-100B using the LIAISON^® S100 assay.","authors":"Jaime I Sainz de Medrano, Judit Julian, Irene Martínez, Anna Escalante, Helena Castellà, Victoria Ortiz, Esther Fernández-Galán, Javier Laguna","doi":"10.1080/00365513.2025.2487978","DOIUrl":"https://doi.org/10.1080/00365513.2025.2487978","url":null,"abstract":"","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":"85 3","pages":"220-222"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parathyroid hormone using second and third generation assays in patients with various stages of chronic kidney disease.","authors":"Ida Marie Nørum Wigh, Anne Kathrine Aagaard Thomsen, Jens Dam Jensen, Hanne Skou Jørgensen, Stine Linding Andersen","doi":"10.1080/00365513.2025.2512998","DOIUrl":"10.1080/00365513.2025.2512998","url":null,"abstract":"<p><p>Mineral metabolism disturbances are common in chronic kidney disease (CKD) and parathyroid hormone (PTH) plays an important role in patient monitoring. Levels of PTH may vary with different biochemical assays, especially with kidney dysfunction, and more evidence is needed to substantiate the differences between second generation (2. gen) and third generation (3. gen) PTH immunoassays in this patient group. A cross-sectional study of 350 CKD patients (61% males) in the North Denmark Region. From April to September 2023, blood samples drawn for measurement of PTH as part of routine care in the Department of Nephrology, Aalborg University Hospital, were consecutively collected for measurement of PTH using different assays (2. gen., Atellica, Siemens Healthineers and 3. gen, Cobas 8000, Roche Diagnostics). Results were reported as the mean relative difference (RD): (2. gen PTH-3. gen PTH)/3. gen PTH. Overall, the 2. gen assay provided higher values of PTH as reflected by the median PTH (2. gen: 26.4 pmol/L; 3. gen 14.5 pmol/L) and the mean RD (82%). When stratified by CKD subgroup, the difference between the assays increased with decreasing kidney function (CKD1-3 (<i>n</i> = 19): mean RD 41%; CKD4 (<i>n</i> = 25): 66%; CKD5 (<i>n</i> = 93): 81%; dialysis (<i>n</i> = 186): 90%). PTH measured with 2. and 3. gen assays differed markedly among CKD patients, and the assay difference was dependent on the degree of kidney failure. Results inform clinical guidance on the monitoring and management based on PTH among patients with CKD.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"313-320"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship of kynurenine pathway metabolites and indoleamine 2,3-dioxygenase with atherosclerosis: data obtained from pericardial fluid in coronary artery bypass surgery.","authors":"Hakan Çomaklı, Mohammad Ahmad Bik, Ali Ünlü, Sedat Abuşoğlu, Mehmet Murat Yiğitbaşı, Mert Demirsöz, Şeref Alp Küçüker","doi":"10.1080/00365513.2025.2565733","DOIUrl":"10.1080/00365513.2025.2565733","url":null,"abstract":"<p><p>Analyzing the local pericardial fluid (PF) surrounding the heart can provide insights into the function of myocardial and pericardial epithelial cells. Kynurenine (KYN) metabolites have been associated with endothelial dysfunction and the progression of atherosclerosis. This study investigated the relationship between KYN pathway metabolites in serum and PF and the severity of coronary atherosclerosis, as assessed by the Syntax Score (SS). The study involved 101 patients (mean age: 60 years). Of the participants, 17% were female. KYN and its metabolite levels were measured using API SCIEX 3200 LC-MS/MS methods. Statistical analysis was performed using IBM SPSS 25.0. The mean serum levels of tryptophan (TRP), 3-hydroxykynurenine (3-HK), and 3-hydroxyanthranilic acid were significantly higher (<i>p</i> < 0.01) when compared to the mean PF levels. In the SS-low group, PF IDO activity was significantly higher than serum IDO activity (<i>p</i> = 0.040). In the SS-high group, PF IDO activity was significantly higher than serum IDO activity (<i>p</i> = 0.002). The mean 3-HK measurements showed significant differences between the SS groups (<i>p</i> < 0.05). In this study, higher IDO enzyme activity in the PF compared to systemic circulation suggests the presence of inflammatory activity in the pericardial endothelial cells. This research aimed to uncover the role of amino acid metabolism, which is poorly understood in atherosclerosis. Specifically, 3-HK, a metabolite from the KYN pathway, may contribute to the development of atherosclerosis. These findings indicate that localized kynurenine pathway activation in the PF may contribute to coronary atherosclerosis, highlighting its potential as a biomarker or therapeutic target.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"509-517"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}