Scandinavian Journal of Clinical & Laboratory Investigation最新文献

Imperfect reference standards cause biased likelihood ratios. 不完善的参考标准导致有偏差的似然比。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-07-12 DOI: 10.1080/00365513.2025.2528214

Arne Åsberg, Ann Elisabeth Åsberg

引用次数: 0

Patient medians for NT-proBNP from six Swedish counties 2011-2021. 2011-2021年瑞典六个县NT-proBNP患者中位数。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-07-04 DOI: 10.1080/00365513.2025.2528220

Morgan Lundgren, Peter Ridefelt, Maria K Eriksson, Thomas Cars, Anders Larsson

{"title":"Patient medians for NT-proBNP from six Swedish counties 2011-2021.","authors":"Morgan Lundgren, Peter Ridefelt, Maria K Eriksson, Thomas Cars, Anders Larsson","doi":"10.1080/00365513.2025.2528220","DOIUrl":"https://doi.org/10.1080/00365513.2025.2528220","url":null,"abstract":"NT-proBNP is crucial in diagnosing and monitoring heart failure. Data from external quality assessment (EQA) schemes reveal existing method differences among manufacturers. Median values of patient results can serve as a quality indicator. The aim was to study if differences between manufacturers seen in EQA data also are evident in patient medians. Over one million NT-proBNP results from adults during 2011-2021 were extracted from six Swedish counties. Results were grouped by method, county, and according to eGFR and diabetes diagnosis. Medians and consensus values were calculated. From 2011 to 2017, Roche accounted for 76% of NT-proBNP results. After 2017, other manufacturers entered and then methods yielded more diverse results. Findings for Roche and Siemens align with a previous EQA study, while Abbott's results differed. Between 2019 and 2021 Roche results were 2% above consensus, Siemens 16% above, Ortho 8% below, and Abbott 11% below. As the number of NT-proBNP requests increased, medians decreased, possibly due to updated guidelines and more widespread testing. Additionally, patients with eGFR < 60 mL/min/1.73m2 BSA or diabetes had medians that were 2-3 times higher compared to the overall results. This study largely confirms varying levels noted in an EQA scheme between manufacturers of NT-proBNP methods, with a notable exception for unknown reasons for Abbott. The expected higher results for patients with eGFR < 60 mL/min/1.73m2 BSA or diabetes reinforced the validity of calculated overall medians. Therefore, patient medians are a clinically relevant quality indicator, and a robust approach to monitor methods as a supplement to internal and external controls.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-11"},"PeriodicalIF":1.3,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guide for the classification of porphyrias using state-of-the-art reverse-phase high-performance liquid chromatography. 使用最先进的反相高效液相色谱法分类卟啉症指南。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-07-02 DOI: 10.1080/00365513.2025.2524714

Javier Laguna, Robin Wijngaard, Lourdes Marés, Marina Parra-Robert, Gregori Casals, Jordi To-Figueras

{"title":"Guide for the classification of porphyrias using state-of-the-art reverse-phase high-performance liquid chromatography.","authors":"Javier Laguna, Robin Wijngaard, Lourdes Marés, Marina Parra-Robert, Gregori Casals, Jordi To-Figueras","doi":"10.1080/00365513.2025.2524714","DOIUrl":"https://doi.org/10.1080/00365513.2025.2524714","url":null,"abstract":"The biochemical diagnosis of porphyria is based on the analysis of porphyrins in urine, feces, and blood using fluorometry and spectrometry. High-performance liquid chromatography (HPLC) with fluorescence detection has been used since the 1980s as standard procedure for separation of porphyrin isomers and classification of the different types of porphyria since each type of porphyria presents a characteristic HPLC isomer distribution either in urine, plasma or feces. We present a unified collection of chromatograms as an aid for porphyria classification in laboratories using HPLC equipment. Biological samples were collected according to approved hospital protocols, and analyzed by reverse-phase HPLC with fluorescence detection, using an unused dedicated chromatographic column BDS-Hypersil™ C18 and reproducing, with minor variations, the conditions originally reported by Lim and Peters in 1984. With the chromatograms, we include a concise explanation of the changes observed. When inter-individual variation is frequent, we include for clarification chromatograms of two different individual samples. Additionally, we present chromatograms showing abnormalities of porphyrin metabolism in patients without porphyria. We add our collection to the literature, as a visual guide to facilitate porphyria diagnosis and classification though understanding of the key metabolic changes. Our aim is to support education of new experts in the porphyria field increasing diagnostic accuracy and ultimately leading to improved patient outcomes and management.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-13"},"PeriodicalIF":1.3,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preanalytical stability of 17 analytes in whole blood during transportation. 17种分析物在全血运输过程中的分析前稳定性。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-06-30 DOI: 10.1080/00365513.2025.2524725

Ida Marie Nørum Wigh, Jesper Erik Karl Staffansson Strandberg, Marianne Christina Harbo Mosgaard, Maja Boe, Mia Séne Lund Christensen, Simon Lykkeboe, Søren Lundbye-Christensen, Aase Handberg, Stine Krogh Venø

{"title":"Preanalytical stability of 17 analytes in whole blood during transportation.","authors":"Ida Marie Nørum Wigh, Jesper Erik Karl Staffansson Strandberg, Marianne Christina Harbo Mosgaard, Maja Boe, Mia Séne Lund Christensen, Simon Lykkeboe, Søren Lundbye-Christensen, Aase Handberg, Stine Krogh Venø","doi":"10.1080/00365513.2025.2524725","DOIUrl":"https://doi.org/10.1080/00365513.2025.2524725","url":null,"abstract":"Reliable biochemical measurements are essential to patient care and rely on the correct preanalytical handling of blood samples. In Denmark, whole blood samples are transported from general practitioners to local hospital laboratories daily, and whole blood stability for 10 h is required for analytes to be included in this transportation service in the North Jutland Region. Therefore, this study aimed to determine the stability of 17 analytes in whole blood during transportation and storage for 6 and 10 h at room temperature (21 °C). Ten randomly selected participants were included in the Department of Clinical Biochemistry at Aalborg University Hospital. Three serum vacutainer tubes were collected from each participant, and 17 analytes (lipoprotein a, immunoglobulin G1, β-2-Microglobulin, κ free light chain, λ free light chain, peptidyl peptidase A, protein, apolipoprotein B, insulin-like growth factor 1, somatotropin, thyroxin binding globulin, anticardiolipin antibodies IgM, prolactin, thyroid peroxidase antibodies, thyroglobulin, thyroglobulin antibodies,17-Hydroxyprogesterone) were measured at baseline, after 6 h, and after 10 h. All analytes showed 6- and 10-hour stability within the limits of the preestablished analytical variation, reflected by coefficients of variation, in the laboratory (accredited by ISO-15189). Thus, transportation and storage of whole blood at room temperature for 6 and 10 h did not result in significant changes in the levels of the 17 studied analytes. Therefore, all analytes were suitable for the transportation service in the North Jutland Region, Denmark.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-6"},"PeriodicalIF":1.3,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Consideration of immunochromatographic strip positive results for screening of alpha-thalassemia in elevated hemoglobin F samples. 免疫层析试纸阳性结果筛选高血红蛋白F样品中-地中海贫血的考虑。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-06-27 DOI: 10.1080/00365513.2025.2524849

Moe Theingi, Pinyaphat Khamphikham, Sakorn Pornprasert

{"title":"Consideration of immunochromatographic strip positive results for screening of alpha-thalassemia in elevated hemoglobin F samples.","authors":"Moe Theingi, Pinyaphat Khamphikham, Sakorn Pornprasert","doi":"10.1080/00365513.2025.2524849","DOIUrl":"https://doi.org/10.1080/00365513.2025.2524849","url":null,"abstract":"The screening test for early detection of α-thalassemia is essential in effective management and genetic counseling. The immunochromatographic (IC) strip test is widely used for α-thalassemia screening due to its simplicity and high sensitivity. This study explores the causes of false-positive IC strip results in subjects with HbF >5% who tested negative for common α0-thalassemia --SEA, --Thai and --Chiang Rai type deletions. Fifty whole blood samples were tested using IC strips, and resulting positive samples were retested with washed red blood cells (RBCs) and plasma. Follow-up testing included molecular analysis to detect common hemoglobinopathies in washed RBCs, including α+-thalassemia -α3.7 and -α4.2 deletions, Hb Constant Spring (CS), Hb Quong Sze (QS) and Hb Westmead (WM), as well as antinuclear antibodies (ANAs) screening in plasma. Ten of 50 EDTA whole-blood samples tested positive using the IC strip test, with eight showing positivity in plasma and seven in washed RBCs. Among them, one plasma-positive sample was also positive for ANA, suggesting potential antibody interference. Of the seven RBC-positive samples, three had common hemoglobinopathies: two with the -α3.7 deletion and one with Hb CS. The remaining four RBC-positive cases had no detectable mutations but were infants under three months of age. Since most false positives occur in infants under 8 months, caution is recommended when testing this age group. Additionally, washing red cells can help reduce antibody interference. Further molecular studies, such as Sanger sequencing, MLPA and NGS, should be initiated in cases without obvious causes.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-7"},"PeriodicalIF":1.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of vacuum and non-vacuum urine collection systems on urinalysis. 真空和非真空尿液收集系统对尿液分析的影响。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-06-26 DOI: 10.1080/00365513.2025.2524850

Berkay Korkmaz, Alev Kural, Ahmet Başman, Bülent Ediz, Abdullah Olgun

{"title":"Effect of vacuum and non-vacuum urine collection systems on urinalysis.","authors":"Berkay Korkmaz, Alev Kural, Ahmet Başman, Bülent Ediz, Abdullah Olgun","doi":"10.1080/00365513.2025.2524850","DOIUrl":"https://doi.org/10.1080/00365513.2025.2524850","url":null,"abstract":"The aim of this study was to investigate the effect of vacuum urine collection system on urine sediment and chemical dipstick analysis results by comparing it with a non-vacuum urine collection system. Vacuum urine collection systems are commonly used due to their user-friendly design, standardization, and low contamination risk, but their impact on the accuracy of urinalysis is not well-documented. A total of 124 urine specimens were collected from patients using vacuum and non-vacuum (Argeron UriCollecT) urine collection systems. The samples were analyzed using Dirui FUS-200 sediment and Dirui H-800 dipstick analyzers within 1 h of collection. Microscopic sediment analyses were confirmed when needed. Microscopic urine sediment analyses showed no significant difference between vacuum and non-vacuum systems for leukocytes, squamous epithelium, non-squamous epithelium, and hyaline casts. However, significant differences (p < 0.05) were observed for red blood cells, crystals, and granular casts. In chemical dipstick analysis, no significant difference was found for hemoglobin, bilirubin, ketone, urobilinogen, protein, nitrite, leukocyte, glucose, and pH. However, significant difference was observed in specific gravity (p < 0.05). Vacuum urine collection systems can cause statistically significant variations in certain sediment and chemical dipstick parameters. These differences should be considered in clinical evaluations, and further research with larger and diverse patient samples is needed to assess the clinical implications and effects on patient outcomes.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-8"},"PeriodicalIF":1.3,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of ABO and Rh(D) blood group on Covid-19 immune response. ABO和Rh(D)血型对Covid-19免疫应答的影响。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-06-20 DOI: 10.1080/00365513.2025.2522673

Erling Englund, Anders E Henriksson

{"title":"The impact of ABO and Rh(D) blood group on Covid-19 immune response.","authors":"Erling Englund, Anders E Henriksson","doi":"10.1080/00365513.2025.2522673","DOIUrl":"10.1080/00365513.2025.2522673","url":null,"abstract":"Previous studies indicate that blood type O has a protective effect against Covid-19 disease. In the present study, we aimed to further explore and clarify the connection between ABO blood groups and the Covid-19 disease by examining the levels of Covid antibodies in patients with different ABO blood types. During the first pandemic year, and before the vaccine was available, SARS-CoV-2 antibody level were randomly analyzed at the regional hospital laboratory and the data was stored in the laboratory information system (LIS) Flexlab/Chemistry. The investigated cohort was created by cross-referencing the SARS-CoV-2 antibody results with the ABO Rh(D) blood group status available in the LIS Prosang. The association between antibody level and blood group was further analyzed within this cohort. There was a significantly higher odds of Covid positivity among individuals in the cohort with blood type A or AB compared to blood type O. There was no significant difference in odds of Covid positivity among between phenotypes B compared to blood type O. The quantitative levels of SARS-CoV-2 antibodies were similar in individuals with a positive Covid-19 test regardless of ABO and Rh(D) phenotype. The present cohort study supports the hypothesis that the presence of anti-A antibodies in serum should be considered as a factor more significant than the blood group itself. However, the study showed a similar immune response in individuals with a positive Covid-19 test regardless ABO phenotype.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-6"},"PeriodicalIF":1.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parathyroid hormone using second and third generation assays in patients with various stages of chronic kidney disease. 甲状旁腺激素使用第二代和第三代测定患者的不同阶段的慢性肾脏疾病。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-05-31 DOI: 10.1080/00365513.2025.2512998

Ida Marie Nørum Wigh, Anne Kathrine Aagaard Thomsen, Jens Dam Jensen, Hanne Skou Jørgensen, Stine Linding Andersen

{"title":"Parathyroid hormone using second and third generation assays in patients with various stages of chronic kidney disease.","authors":"Ida Marie Nørum Wigh, Anne Kathrine Aagaard Thomsen, Jens Dam Jensen, Hanne Skou Jørgensen, Stine Linding Andersen","doi":"10.1080/00365513.2025.2512998","DOIUrl":"https://doi.org/10.1080/00365513.2025.2512998","url":null,"abstract":"Mineral metabolism disturbances are common in chronic kidney disease (CKD) and parathyroid hormone (PTH) plays an important role in patient monitoring. Levels of PTH may vary with different biochemical assays, especially with kidney dysfunction, and more evidence is needed to substantiate the differences between second generation (2. gen) and third generation (3. gen) PTH immunoassays in this patient group. A cross-sectional study of 350 CKD patients (61% males) in the North Denmark Region. From April to September 2023, blood samples drawn for measurement of PTH as part of routine care in the Department of Nephrology, Aalborg University Hospital, were consecutively collected for measurement of PTH using different assays (2. gen., Atellica, Siemens Healthineers and 3. gen, Cobas 8000, Roche Diagnostics). Results were reported as the mean relative difference (RD): (2. gen PTH-3. gen PTH)/3. gen PTH. Overall, the 2. gen assay provided higher values of PTH as reflected by the median PTH (2. gen: 26.4 pmol/L; 3. gen 14.5 pmol/L) and the mean RD (82%). When stratified by CKD subgroup, the difference between the assays increased with decreasing kidney function (CKD1-3 (n = 19): mean RD 41%; CKD4 (n = 25): 66%; CKD5 (n = 93): 81%; dialysis (n = 186): 90%). PTH measured with 2. and 3. gen assays differed markedly among CKD patients, and the assay difference was dependent on the degree of kidney failure. Results inform clinical guidance on the monitoring and management based on PTH among patients with CKD.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-8"},"PeriodicalIF":1.3,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-sampling of capillary blood for safety monitoring of DMARD therapy in patients with rheumatic disease: a feasibility and method-comparison study. 自采毛细血管血液用于风湿病患者DMARD治疗的安全监测：可行性和方法比较研究

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-04-01 Epub Date: 2025-02-07 DOI: 10.1080/00365513.2025.2463087

Josefine Bak H Adelhelm, Trine Rennebod Larsen, Ulla Jakobsen, Pernille J Vinholt, Maria Boysen Sandberg, Inger Marie Jensen Hansen, Søren Andreas Just

{"title":"Self-sampling of capillary blood for safety monitoring of DMARD therapy in patients with rheumatic disease: a feasibility and method-comparison study.","authors":"Josefine Bak H Adelhelm, Trine Rennebod Larsen, Ulla Jakobsen, Pernille J Vinholt, Maria Boysen Sandberg, Inger Marie Jensen Hansen, Søren Andreas Just","doi":"10.1080/00365513.2025.2463087","DOIUrl":"10.1080/00365513.2025.2463087","url":null,"abstract":"Our study aimed to compare the quality of patient self-collected capillary samples with venous blood samples. Additionally, we assessed whether patients with rheumatic disease are both capable of and willing to perform capillary self-sampling through subjective and objective assessments. This research explores the future potential of at-home self-sampling. Patients with rheumatic diseases were asked to perform up to four supervised self-collected capillary blood samples, followed by a standard venous sample performed by study personnel. Anti-rheumatic drug treatment monitoring parameters, including biochemistry and hematology, were analyzed using Cobas 8000 and Sysmex XN-9000, respectively. The agreement was evaluated by Bland-Altman plots and compared to critical difference limits. Study personnel and patients answered a survey questionnaire after every visit to evaluate feasibility. In total, 21 patients completed 53 paired capillary and venous samples from November 2019 to December 2020. We found a strong correlation (r > 0.87) and good agreement for most parameters; platelets showed the poorest agreement. Patients experienced little pain, found self-sampling easy and reported no serious complications. Hemolysis affected 12/53 capillary biochemistry samples, and 5/53 capillary hematology samples coagulated. The good agreement for most parameters and excellent feasibility encourages the potential for capillary self-sampling of DMARD monitoring parameters, relevant limitations were hemolysis and aggregating platelets.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"108-115"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of large language models in medical quizzes for clinical chemistry and laboratory management: implications and applications for healthcare artificial intelligence. 临床化学和实验室管理医学测验中大型语言模型的评估：对医疗人工智能的影响和应用。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2025-04-01 Epub Date: 2025-02-19 DOI: 10.1080/00365513.2025.2466054

Won Young Heo, Hyung-Doo Park

{"title":"Assessment of large language models in medical quizzes for clinical chemistry and laboratory management: implications and applications for healthcare artificial intelligence.","authors":"Won Young Heo, Hyung-Doo Park","doi":"10.1080/00365513.2025.2466054","DOIUrl":"10.1080/00365513.2025.2466054","url":null,"abstract":"Large language models (LLMs) have demonstrated high performance across various fields due to their ability to understand, generate, and manipulate human language. However, their potential in specialized medical domains, such as clinical chemistry and laboratory management, remains underexplored. This study evaluated the performance of nine LLMs using zero-shot prompting on 109 clinical problem-based quizzes from peer-reviewed journal articles in the Laboratory Medicine Online (LMO) database. These quizzes covered topics in clinical chemistry, toxicology, and laboratory management. The models, including GPT-4o, Claude 3 Opus, and Gemini 1.5 Pro, along with their earlier or smaller versions, were assigned roles as clinical chemists or laboratory managers to simulate real-world decision-making scenarios. Among the evaluated models, GPT-4o achieved the highest overall accuracy, correctly answering 81.7% of the quizzes, followed by GPT-4 Turbo (76.1%), Claude 3 Opus (74.3%), and Gemini 1.5 Pro (69.7%), while the lowest performance was observed with Gemini 1.0 Pro (51.4%). GPT-4o performed exceptionally well across all quiz types, including single-select, open-ended, and multiple-select questions, and demonstrated particular strength in quizzes involving figures, tables, or calculations. These findings highlight the ability of LLMs to effectively apply their pre-existing knowledge base to specialized clinical chemistry inquiries without additional fine-tuning. Among the evaluated models, GPT-4o exhibited superior performance across different quiz types, underscoring its potential utility in assisting healthcare professionals in clinical decision-making.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"125-132"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0