Scandinavian Journal of Clinical & Laboratory Investigation最新文献

{"title":"Paediatric, age-specific reference intervals for reticulocyte count on Abbotts Alinity hq platform.","authors":"Rie H Nygaard, Rie McGrail, Cristine Betzer","doi":"10.1080/00365513.2025.2569832","DOIUrl":"https://doi.org/10.1080/00365513.2025.2569832","url":null,"abstract":"<p><p>Since haematopoiesis changes dramatically after birth and during childhood, specific paediatric reference intervals are critical for meaningful interpretation of haematological blood analyses. Paediatric reference intervals for reticulocyte count are not available for Abbott platforms. In this study, we used an indirect, data-mining approach with data from Abbott's Alinity hq platform to establish reticulocyte count reference intervals for children aged up to 18 years. Data were extracted from the 1^st of July 2021 to the 6^th of November 2024 and in order to find the 'healthiest patients', we included only reticulocyte counts from samples with normal haemoglobin and only samples from patients with a single reticulocyte count in the extraction period (<i>n</i> = 1633). We found relatively high reticulocyte counts in the first week of life (reference intervals for age 0-<7 days: 74-442 *10^9/L) and similar reticulocyte counts after this period and throughout childhood (reference interval for age 7 days-<18 years: 32-115 *10^9/L). In conclusion, this is the first study to report reference intervals for reticulocyte count in children using an Abbott platform. Compared to the literature, our reference intervals are higher than the reference intervals on other platforms, which is in line with reference intervals for adults, where Abbott also reports higher values compared to other platforms. The established reference interval for reticulocytes offers valuable insights for other laboratories using the Alinity hq platform and enhances the laboratory's ability to support clinical decision-making.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-5"},"PeriodicalIF":1.4,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between serum MST1 and the prognosis in patients with chronic rhinosinusitis with nasal polyps after functional endoscopic sinus surgery.","authors":"Ming Yang, Haohua Zhu, Qian Liu","doi":"10.1080/00365513.2025.2565821","DOIUrl":"https://doi.org/10.1080/00365513.2025.2565821","url":null,"abstract":"<p><p><b>Background and Aims</b>: Chronic rhinosinusitis with nasal polyps (CRSwNP) is prone to postoperative recurrence. This study aimed to investigate the association between serum mammalian Ste20-like kinase 1 (MST1) and prognosis in CRSwNP patients after functional endoscopic sinus surgery (FESS). <b>Methods</b>: This prospective observational study included 100 CRSwNP patients undergoing FESS and 100 patients with nasal septal deviation or maxillary sinus cysts as controls. Serum levels of MST1, IFN-γ, and IL-5 were measured using Enzyme-linked immunosorbent assay (ELISA). MST1 mRNA expression in nasal polyps and inferior turbinate samples was quantified by qRT-PCR. Sinus lesions and quality of life were assessed using the Lund-Mackay, Lund-Kennedy, and SNOT-22 scoring systems. The 1-year recurrence-free survival was analyzed using Kaplan-Meier (K-M) curve, and the diagnostic value of MST1 for CRSwNP was evaluated by Receiver operating characteristic (ROC) curve. <b>Results</b>: Serum MST1 levels and MST1 mRNA expression were significantly elevated in CRSwNP patients. High MST1 expression positively correlated with increased eosinophils percentage and elevated IL-5 levels. Moreover, patients with lower MST1 expression exhibited better clinical outcomes and a lower recurrence rate. Logistic regression analysis identified MST1 and allergic rhinitis as independent predictors of recurrence. Furthermore, MST1 demonstrated a strong predictive value for recurrence in CRSwNP patients. <b>Conclusion</b>: Elevated serum MST1 levels were strongly associated with inflammation, disease severity, and recurrence risk in CRSwNP. These findings suggest that MST1 could serve as a valuable prognostic biomarker for postoperative outcomes in CRSwNP patients.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-7"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revelations from charge-balance modeling.","authors":"Troels Ring, Stephen Edward Rees, Sebastian Frische","doi":"10.1080/00365513.2025.2559345","DOIUrl":"https://doi.org/10.1080/00365513.2025.2559345","url":null,"abstract":"<p><p>In biological fluids the charge is dominated by strong ions with pH-independent complete dissociation. The requirement of electroneutrality in combination with the principle of mass conservation and rules of dissociation as understood from physical chemistry including auto-dissociation of water allows definite specification of charge in any water-based fluid with known composition. Herein, two important publications are revisited using charge-balance modeling. In the first case it is shown that knowing a fixed pH difference between two types of fibroblasts and knowing also the pH-dependent buffer-capacities in the two cells allows quantitative assessment of buffer concentrations and strong ion differences. In the second example it is shown that the difficult problem of finding by calculation or titration the titratable acidity in urine modeled as a phosphate solution with high ionic strength can be solved with just measuring urine SID and total phosphate concentration without knowing either the correct dissociation constant or pH in urine. It is suggested that charge-balance modeling based on basic physical chemistry may reveal non-trivial ontological details of the system under study.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-10"},"PeriodicalIF":1.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical performance and user-friendliness of cobas pulse glucose meter in the hands of the intended users.","authors":"Christine Morken, Joakim Hekland, Johan Olausson, Johan Baden Birk-Korch, Heidi Berghäll, Johannes Østrem Fjøse, Gunhild Øygard Fosse, Sander Johannes Thorbjørnsen Guttorm, Gustav Hallén, Anne Haapala, Tine-Lise Kalleklev, Stiina Kamula, Dår Kristian Kur, Kristiina Kurg, Cindhya Sithiravel, Anne Elisabeth Solsvik, Ricardas Stonys, Kristel Virtanen, Elisabeth Wiik Vigerust, Anne Stavelin","doi":"10.1080/00365513.2025.2565732","DOIUrl":"https://doi.org/10.1080/00365513.2025.2565732","url":null,"abstract":"<p><p>Diabetes is a growing global health concern affecting millions worldwide. Glucose measurement remains a cornerstone in the diagnosis, monitoring, and management of this disease. Considering that numerous point-of-care (POC) devices for glucose measurements are available, systematic evaluation of their analytical performance and user-friendliness is essential. This study presents the findings of an independent evaluation of the cobas pulse glucose meter (Roche Diagnostics GmbH) conducted by the Scandinavian evaluation of laboratory equipment for point of care testing (SKUP). The study, which assessed both the analytical performance and user-friendliness, included 217 participants with diabetes and was conducted in both a hospital laboratory and primary healthcare settings. Capillary whole blood measurements were compared to a plasma glucose hexokinase method at Vejle hospital, Denmark. Certified reference materials from the National Institute of Standards and Technology (NIST) were used to correct all sample results. Repeatability and accuracy were assessed against predefined analytical performance specifications, and user-friendliness was evaluated using a structured questionnaire developed by SKUP. The cobas pulse demonstrated good repeatability (CV: 1.8-4.0%) and fulfilled the specification for accuracy, with minimal bias (maximum +0.16 mmol/L in the 7-10 mmol/L range). Haematocrit had no significant effect within the tested range (5-70%). User-friendliness was rated satisfactory, attributed to intuitive operation, rapid analysis times, and effective quality control features. In conclusion, the cobas pulse glucose meter met the performance criteria for professional use in clinical and primary care settings, supporting its suitability in near-patient glucose monitoring.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic drug monitoring of pharmacologically active ampicillin, cefuroxime, meropenem, tazobactam and piperacillin - method validation and results from one-year experience.","authors":"Mette Christensen, Lise Bathum, Klaus Tjelle Kristiansen, Ditte Gry Strange, Mette Pinholt, Sara Thønnings","doi":"10.1080/00365513.2025.2565726","DOIUrl":"https://doi.org/10.1080/00365513.2025.2565726","url":null,"abstract":"<p><p>Therapeutic drug monitoring (TDM) is an important tool for securing optimal treatment of drugs like beta-lactam antibiotics in hospitalized patients. Developing accurate and robust methods for quantification of the pharmacologically active fraction is needed. A LC-MS/MS assay, using ultrafiltration for selection of the non-protein bound (i.e. pharmacologically active) fraction followed by a 5.5 min chromatographic separation was developed and validated for simultaneous determination of Ampicillin (AMPI), Cefuroxime (CEFU), Meropenem (MERO) and Piperacillin (PIPE)/Tazobactam (TAZO). Inter-batch imprecision was <10%. Recovery was 88-115% from lowest level of quantification (LLOQ) to 2500 mg/L for MERO, PIPE and TAZO and LLOQ-500 mg/L for AMPI and CEFU. Storage temperature had great impact on measured concentrations, with PIPE being most instable. No effect of temperature during ultrafiltration was found for the measured beta-lactams. When evaluating one-year routine clinical use of the analysis, representing 581 (mainly ICU) patient samples, >50% of patients treated with PIPE (and AMPI), and about 30% treated with MERO and CEFU, exhibited beta-lactam levels higher than the local guidelines for maximum recommended levels. Potential toxic levels were found in several patients. We believe that the analytical method developed in this study represents an accurate measure of the pharmacologically active beta-lactam fraction. Evaluation of patient data after one-year use of the method shows higher than expected beta-lactam levels, highlighting the potential benefit of TDM. More studies are needed to determine the clinical significance of TDM in beta-lactam antibiotics.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-11"},"PeriodicalIF":1.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship of kynurenine pathway metabolites and indoleamine 2,3-dioxygenase with atherosclerosis: data obtained from pericardial fluid in coronary artery bypass surgery.","authors":"Hakan Çomaklı, Mohammad Ahmad Bik, Ali Ünlü, Sedat Abuşoğlu, Mehmet Murat Yiğitbaşı, Mert Demirsöz, Şeref Alp Küçüker","doi":"10.1080/00365513.2025.2565733","DOIUrl":"https://doi.org/10.1080/00365513.2025.2565733","url":null,"abstract":"<p><p>Analyzing the local pericardial fluid (PF) surrounding the heart can provide insights into the function of myocardial and pericardial epithelial cells. Kynurenine (KYN) metabolites have been associated with endothelial dysfunction and the progression of atherosclerosis. This study investigated the relationship between KYN pathway metabolites in serum and PF and the severity of coronary atherosclerosis, as assessed by the Syntax Score (SS). The study involved 101 patients (mean age: 60 years). Of the participants, 17% were female. KYN and its metabolite levels were measured using API SCIEX 3200 LC-MS/MS methods. Statistical analysis was performed using IBM SPSS 25.0. The mean serum levels of tryptophan (TRP), 3-hydroxykynurenine (3-HK), and 3-hydroxyanthranilic acid were significantly higher (<i>p</i> < 0.01) when compared to the mean PF levels. In the SS-low group, PF IDO activity was significantly higher than serum IDO activity (<i>p</i> = 0.040). In the SS-high group, PF IDO activity was significantly higher than serum IDO activity (<i>p</i> = 0.002). The mean 3-HK measurements showed significant differences between the SS groups (<i>p</i> < 0.05). In this study, higher IDO enzyme activity in the PF compared to systemic circulation suggests the presence of inflammatory activity in the pericardial endothelial cells. This research aimed to uncover the role of amino acid metabolism, which is poorly understood in atherosclerosis. Specifically, 3-HK, a metabolite from the KYN pathway, may contribute to the development of atherosclerosis. These findings indicate that localized kynurenine pathway activation in the PF may contribute to coronary atherosclerosis, highlighting its potential as a biomarker or therapeutic target.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cystatin C and creatinine eGFR reference intervals in healthy adults from Cameroon.","authors":"Bertil Kågedal, Dženeta Nezirević Dernroth, Bertille Elodie Edinga-Melenge, Adrienne Yakam Tchapmi, Jobert Richie Nansseu, Vicky Jocelyne Ama Moor, Suzanne Belinga, Håkan Hanberger, Anders Helldén","doi":"10.1080/00365513.2025.2559354","DOIUrl":"https://doi.org/10.1080/00365513.2025.2559354","url":null,"abstract":"<p><p>Several equations are available for estimation of glomerular filtration rates (eGFRs) from serum cystatin C and creatinine, but data on reference intervals from healthy subjects are scarce. Employing nine well-known equations, we calculated eGFRs for healthy Cameroonians, 194 men and 187 women 18-71 years of age. Reference intervals were calculated using conventional techniques. For subjects < 50 years of age the eGFR reference intervals were with CAPA (men; women) 70.1-127.1; 79.9-172.2, with CKD-EPI_{cys 2012} 75.1-131.2; 81.3-135.5, with LM rev 65.4-106.5; 61.4-110.3, with CKD-EPI_{crea 2021} 74.1-127.2; 67.0-129.8, with mean (LM rev, CAPA) 72.2-110.6; 72.8-136.9, with CKD-EPI_{crea, cys 2021} 81.1-126.6; 77.0-135.7, with EKFC_cys 85.5-116.3; 83.3-124.8, with EKFC_crea 69.4-114.2; 62.8-114.3, and with EKFC_{crea, cys} 82.5-114.0: 77.1-116.4 mL/min/1.73 m², respectively. Median eGFR_cys was significantly higher than eGFR_crea irrespective of equations used. The eGFR_cys difference between healthy men and women aged < 50 years may be explained by lower production rates of cystatin C in women. The higher eGFR_cys reference intervals compared to that of eGFR_crea may be caused by increased tubular secretion of creatinine or increased production of cystatin C in the cohorts used for establishing the equations relative the case in healthy subjects. This suggests that the eGFR equations need to be further assessed regarding accuracy to adequately correspond to true GFR.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-12"},"PeriodicalIF":1.4,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of point-of-care instrument for quantification of infliximab and adalimumab.","authors":"Mark Ainsworth, Torben Knudsen, Karina Vogn Koch, Mads Nybo, Morten Beck Trelle","doi":"10.1080/00365513.2025.2559355","DOIUrl":"https://doi.org/10.1080/00365513.2025.2559355","url":null,"abstract":"<p><strong>Introduction: </strong>Treatment with infliximab (IFX) and adalimumab (ADL) are used in a range of inflammatory diseases. Measurement of drug levels is warranted, but can be challenged by turn-around-times at the laboratories. Consequently, point-of-care testing (POCT) technology is becoming increasingly relevant. We evaluated the precision and comparability of results obtained with ProciseDx (Biosynex) when used in outpatient gastroenterology clinics monitoring IFX and ADL.</p><p><strong>Materials and methods: </strong>In this prospective multi-center study, capillary and venous blood samples were collected by trained nurses from patients with inflammatory bowel disease on maintenance therapy with either IFX or ADL. Fourteen different nurses performed blood sampling and IFX measurements using ProciseDx on 64 patients, while 11 different nurses performed ADL measurements on 48 patients. Venous samples were sent to the laboratory for routine testing using Promonitor ELISA kit on a Triturus (Grifols).</p><p><strong>Results: </strong>Across all patients and all sites, a variation of 18.9% and 11.4% was observed for IFX and ADL measurements, respectively. Peak variance was in the 5-10 mg/L IFX concentration range, while peak variance for ADL measurements was above 12 mg/L. Compared to the routine ELISA, the <i>r</i>-value was 0.82 for IFX with a mean total deviation of 2.10 mg/L (27.1%). For ADL, the <i>r</i>-value was 0.91 with a mean total deviation of 2.93 mg/L (39.7%).</p><p><strong>Conclusion: </strong>We find the efficacy and accuracy of the ProciseDx acceptable, but when performed by non-laboratory personnel, the differences to routine measurements are considerable and could have a clinical impact. Clinical implementation would at least require reevaluation of the therapeutic intervals.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age- and sex-specific reference values for FIB-4 derived from the Nordic Reference Interval Project (NORIP).","authors":"Lena Hamelius, Gunnar Nordin, Per Bjellerup, Anders Larsson","doi":"10.1080/00365513.2025.2559352","DOIUrl":"https://doi.org/10.1080/00365513.2025.2559352","url":null,"abstract":"<p><p><b>Introduction</b>: The Fibrosis-4 (FIB-4) index is a non-invasive biomarker that is widely used to assess liver fibrosis. A limitation is that the enzyme methods (aspartate transaminase (AST) and alanine transaminase (ALT)) are not specified in studies. The aim of the present study was to define reference values for FIB-4 index using Nordic Reference Interval Project data where the results for P-AST and P-ALT are traceable to the IFCC reference measurement procedures. <b>Material and methods</b>: FIB-4 values from 1161 subjects (550 males and 611 females) in the NORIP study were used to calculate reference intervals for the index. The 2.5th and 97.5th percentiles for these markers were calculated according to the International Federation of Clinical Chemistry guidelines. <b>Results</b>: Age (18-<39.9, 40-59.9 and 60-85 years) and sex-specific reference intervals were calculated. Of the components used to calculate the FIB-4 index the age had the greatest impact. In the cohort older than 60 years, 7.5% of the females and 18.2% of the males were above the 2.67 limit for high risk of advanced fibrosis. In the same age group 84.2% of the females and 77.6% of the males had FIB-4 values above the 1.3 limit for intermediate risk and further evaluation required. These are very high proportions which means that a very large proportion of the patients will have false positive results when testing populations with a low disease prevalence. <b>Conclusion</b>: FIB-4 index increases with age, potentially leading to overestimation of fibrosis in elderly patients if age specific decision values are not used.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-6"},"PeriodicalIF":1.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening efficacy of erythroferrone and hepcidin in identifying thalassemia with iron-deficiency anemia among pregnant women.","authors":"Nan Huang, Jianlong Zhuang, Yufang Wang, Xiaofeng Zheng, Zixuan Chen, Hailong Huang, Zhishan Zhang","doi":"10.1080/00365513.2025.2553294","DOIUrl":"https://doi.org/10.1080/00365513.2025.2553294","url":null,"abstract":"<p><p>This study investigated the prevalence of Thalassemia (Thal) gene in pregnant women in the Quanzhou area, China. And explored the clinical application value of Erythroferrone (ERFE) and Hepcidin in screening pregnant women with Thal complicated by Iron-deficiency anemia (IDA, defined as serum ferritin (SF) < 20 μg/L). From January 2020 to December 2022, the detection rate of Thal in suspected Thal populations was 35.9%, with 256 pregnant women having Thal, and 11.3% of them also had IDA. From April 2023 to September 2024, 50 pregnant women with simple IDA and 54 with Thal complicated by IDA were selected. Blood samples were collected to detect ERFE, Hepcidin, and other indicators. Binary logistic regression analysis showed that ERFE, Hepcidin, MCH, and SF had a significant impact on Thal screening. The combined detection of these four indicators had the highest AUC at 0.916 (sensitivity 92.0%, specificity 81.5%). The study concluded that pregnant women with Thal are at risk of developing IDA, and routine screening for Thal complicated by IDA is prone to miss cases. ERFE and Hepcidin have higher screening value than MCH and SF, and the combined detection of the four indicators provides high diagnostic efficacy in distinguishing simple IDA from Thal complicated by IDA.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"1-8"},"PeriodicalIF":1.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}