Revista espanola de fisiologia最新文献_第5页

A potential experimental model for the study of osteopenia in CCl4 liver cirrhotic rats. CCl4型肝硬化大鼠骨质减少研究的潜在实验模型。

Revista espanola de fisiologia Pub Date : 1996-06-01

B Muguerza, C Lecároz, A Picardi, I Castilla-Cortázar, J Quiroga, A Cemborain, J Prieto, S Santidrián

{"title":"A potential experimental model for the study of osteopenia in CCl4 liver cirrhotic rats.","authors":"B Muguerza, C Lecároz, A Picardi, I Castilla-Cortázar, J Quiroga, A Cemborain, J Prieto, S Santidrián","doi":"","DOIUrl":"","url":null,"abstract":"In order to search for an experimental model to further investigate the osteopenia associated to liver cirrhosis (LC), this study has been focused on investigating the occurrence of bone disorders in male rats to which LC histologically confirmed was induced through the validated procedure of CCl4 inhalation. Length, anteroposterior and lateromedial diameters, densitometry, mechanical stress resistance, hydroxyproline (OHprol) and calcium and phosphate contents were measured in femurs from control (n = 10) and liver cirrhosis rats (n = 10). It has been found that femurs from liver cirrhosis rats showed a significant reduction (p < 0.01) in bone weight (0.254 +/- 0.003 vs 0.230 +/- 0.004 g/100 g b.w.), anteroposterior (4.08 +/- 0.06 vs 3.69 +/- 0.05 mm) and lateromedial (5.33 +/- 0.05 vs 5.08 +/- 0.04 mm, p < 0.05) diameters, resistance to mechanical stress (405.8 +/- 9.5 vs 332.5 +/- 9.1 N) and total densitometry (0.416 +/- 0.005 vs 0.381 +/- 0.004 g/cm2). However, no significant differences were observed in bone length, calcium, OHprol and phosphate (all expressed as mg/100 mg fresh bone tissue) contents. Therefore, the proteins matrix to mineral contents ratio was not altered. These results indicate that in this model of experimental liver cirrhosis there is osteopenia characterized by bone frailty and reduced thickness, and it could offer an experimental model to study bone changes associated to liver cirrhosis.","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"52 2","pages":"113-9"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19836772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic determination of coat color affects testicular steroidogenesis in the Mustela vison. 毛色的遗传决定影响睾丸甾体激素的形成。

Revista espanola de fisiologia Pub Date : 1996-06-01

A G Amador, C Sundqvist, A Bartke

引用次数: 0

Role of fragment peptides from ob protein on body weight and thermogenesis control: a screening. ob蛋白片段肽在体重和产热控制中的作用:筛选。

Revista espanola de fisiologia Pub Date : 1996-06-01

J A Martínez, M García-Granero, G Frühbeck

引用次数: 0

Development of a simple, rapid sandwich enzyme immunoassay for the measurement of serum rat LH. 建立一种简便、快速测定大鼠血清LH的夹心酶免疫分析法。

Revista espanola de fisiologia Pub Date : 1996-06-01

J C Illera, C J Munro, G Silvan, R H BonDurant, M Illera

{"title":"Development of a simple, rapid sandwich enzyme immunoassay for the measurement of serum rat LH.","authors":"J C Illera, C J Munro, G Silvan, R H BonDurant, M Illera","doi":"","DOIUrl":"","url":null,"abstract":"The present study describes the development and validation of a rapid, sensitive, specific and precise enzyme immunoassay (EIA) sandwich suitable for measuring luteinizing hormone (LH) in rat serum. Ninety-six well polystyrene microtiter plates were coated with 100 microliters (250 ng/ml) of a well-characterized monoclonal antibody (518B7, Roser, UC Davis) generated against bovine LH. A polyclonal antiserum raised in rabbits against ovine FSH (G4-215B, Papkoff) was conjugated to sodium periodate-activated horseradish peroxidase (HRP), and used as the second antibody of the sandwich assay. This anti-ovine FSH antiserum cross-reacted more than 200% with rat LH. Standards (r-LH-RP-3, NIADDK, range 0 pg/well to 2.5 ng/well or 100 microliters) diluted in a 3(N-Morpholino) propane sulfonic acid (MOPS) buffer, or serum, were incubated with the solid phase antibody for 2 hours. Plates were washed and the anti-oFSH:HRP (100 microliters) in MOPS buffer was added and incubated a further 2 hours before a second wash and the addition of the substrate (TMB, 3,3',5,5'-tetramethylbenzidine dihydrochloride and H2O2). The least detectable concentration of LH was 16.1 +/- 1.42 pg/ml. The recovery of known concentrations of LH added to several samples was 93.5 +/- 1.70%. Mean intra-assay and inter-assay coefficients of variation (%) were less than 10% (n = 20). The anti-FSH:HRP showed less than 8.0% cross reactivity with rFSH in this LH EIA system. The correlation coefficient (r) of samples analyzed by EIA in parallel with RIA was r = 0.90 (p < 0.001, n = 26). Results showed levels between 105.21 and 633.87 pg/ml. This new LH EIA sandwich offers a stable, rapid, and improved EIA system for the measurement of serum LH concentrations of this species over previously reported methods.","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"52 2","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19835529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of osmolarity on the epithelial paracellular permeability in rat jejunum. 渗透压对大鼠空肠上皮细胞旁通透性的影响。

Revista espanola de fisiologia Pub Date : 1996-06-01

M Pérez, A Barber, F Ponz

{"title":"Effect of osmolarity on the epithelial paracellular permeability in rat jejunum.","authors":"M Pérez, A Barber, F Ponz","doi":"","DOIUrl":"","url":null,"abstract":"Studies in vivo have shown an important increase in the substrate passive permeability across small intestine when Na(+)-cotransported substrates, like galactose, are present at the luminal side. The influence of the solution osmolarity on the passive absorption of mannitol or 2-deoxy-D-glucose across rat jejunum and on the galactose-increasing effect is now studied both in vivo and in vitro. In vivo, luminal perfusion with 400 or 500 mosm/L solutions does not affect passive absorption of 10 mmol/L 2-deoxy-D-glucose, although a net fluid secretion towards lumen is observed. Luminal hyperosmolarity, however, prevents the stimulatory action of 25 mmol/L galactose on the passive absorption, a stimulation that is well manifested in the same intestinal segment when the perfusion is made with isoosmotic solutions. However, in vitro results with everted intestinal sacs or with preparations of intestinal wall in Ussing chambers, indicate that hyperosmolarity (500 mosm/L) of the solutions clearly increases net passive mucosal to serosal flux of D-mannitol or 2-deoxyglucose. With low osmolarity solutions (180 or 160 mosm/L by diminution of NaCl) the in vivo passive mannitol absorption is not affected and the stimulatory action of 25 mmol/L galactose is not observed, although net water absorption is enhanced. Moreover, the passive absorption stimulation by the galactose cotransport in isoosmotic solutions is dependent on Na+ levels requiring at least 80 mmol/L. Results suggest that passive paracellular absorption across the small intestine may be modified by changes in the luminal content composition.","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"52 2","pages":"103-12"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19836771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human red cells from prenatal stages of hemopoiesis. Lithium flux components. 产前造血阶段的人类红细胞。锂熔剂组件。

Revista espanola de fisiologia Pub Date : 1996-06-01

J L Corchs, G Mujica, R E Serrani

引用次数: 0

Assessment of the energetic role of endogenous substrates in the tail artery of rat by means of enzyme inhibitors. 利用酶抑制剂评价内源性底物在大鼠尾动脉中的能量作用。

Revista espanola de fisiologia Pub Date : 1996-03-01

E A Savino, A Varela, M Carregal

引用次数: 0

Dietary intervention in a hypercholesterolemic school-aged population from Northern Spain. 西班牙北部高胆固醇学龄人群的饮食干预

Revista espanola de fisiologia Pub Date : 1996-03-01

A García, J M Gutiérrez, S Fernández, J Aparicio, A Menéndez-Patterson

{"title":"Dietary intervention in a hypercholesterolemic school-aged population from Northern Spain.","authors":"A García, J M Gutiérrez, S Fernández, J Aparicio, A Menéndez-Patterson","doi":"","DOIUrl":"","url":null,"abstract":"A \"before and after\" intervention study, over a five year period, was done in order to find the degree of compliance to dietary recommendations and their effects on serum lipids in a hypercholesterolemic school-aged population. 383 children between 6 and 13 years of age, were identified with hypercholesterolemia (total serum levels above 4.8 mmol/l), in a previous randomized mass screening of 1095 individuals. Some dietary guidelines aimed at decreasing fat consumption and increasing fiber and carbohydrate ingestion were given to the families, and 226 of the children participated in the follow-up. Changes of address and/or school-center was the main cause of non-participation. The follow-up examination consisted in the evaluation of serum lipids and actual diet. Cholesterol serum levels decreased from a mean value of 5.4 mmol/l to 4.8 mmol/l. 59.3% of the sample had reached serum cholesterol levels below the risk threshold. Fat ingestion diminished significantly (40.3% of calories to 36.7%, p < 0.001), saturated fatty acids decreased from 15.3% of calories to 13.5% (p < 0.001), monounsaturated fatty acids decreased from 17.1% to 15.4% (p < 0.001) and polyunsaturated fatty acids remained unchanged. Carbohydrate consumption increased from 45% of the calories to 47.38% (p < 0.001). Dietary changes were greater in individuals out of risk than those who still had high cholesterol levels. These data indicate a good family compliance to the recommendations and that changes in food habits seem to be effective in controlling hyperlipidemia. Dietary changes are better accepted by younger children.","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"52 1","pages":"49-58"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19747053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of cephalosporins on intestinal enzymatic activity. 头孢菌素对肠道酶活性的影响。

Revista espanola de fisiologia Pub Date : 1996-03-01

J Jordán, I Idoate, E Urdaneta, J Larralde

引用次数: 0

A simple unified explanation of the red-ox and acid-base concepts. 对红牛和酸碱概念的简单统一的解释。

Revista espanola de fisiologia Pub Date : 1996-03-01

J M Macarulla

引用次数: 0