Revista latinoamericana de microbiologia最新文献_第6页

Regulatory considerations of aflatoxin contamination of food in Mexico. 墨西哥食品中黄曲霉毒素污染的监管考虑。

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Doralinda Guzmán-de-Peña, Juan José Peña-Cabriales

引用次数: 0

Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina. 阿根廷东北部野外捕获的埃及伊蚊中登革热病毒的分子检测。

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Domingo Javier Liotta, Gustavo Cabanne, Rodolfo Campos, Sergio Andrés Tonon

引用次数: 0

[Evolution and phylogeny of rhizobia]. 根瘤菌的进化与系统发育

Revista latinoamericana de microbiologia Pub Date : 2005-01-01

Lourdes Lloret, Esperanza Martínez-Romero

{"title":"[Evolution and phylogeny of rhizobia].","authors":"Lourdes Lloret, Esperanza Martínez-Romero","doi":"","DOIUrl":"","url":null,"abstract":"Nitrogen fixation an ancient process that may is have originated in the archaean Eon under the primitive atmosphere anoxygenic conditions. Diazotrophy is an exclusive process of prokaryotes, only Euryarchaeota and 6 of 54 Bacteria phyla have diazotrophs lineages. Some of them coevolved with flowering plants for the establishment of molecular bases of a mutualistic symbiosis relationship. In rhizobia, the nitrogen fixation occurs inside the nodules, special structures on the roots or stems of legumes. Nodule organogenesis starts with the bacterial nodulation factors (Nod factors) codified in large plasmids or symbiotic islands in the bacterial genomes. Nodulation genes had more recent origin than the nitrogen fixation ones because the origin of the nod gene is associated with the origin of the hosts. The 16S rRNA phylogeny groups rhizobia in 7 genuses of the alpha-Proteobacteria: Bradyrhizobium, Mesorhizobium, Rhizobium, Sinorhizobium, Methylobacterium and Devosia, and two genuses recently described in f-Proteobacteria: Burkholderia and Wautersia. The phylogenies obtained with other chromosomal genes are similar at the genus level, but it is incongruent with the symbiotic gene (nif & nod) phylogeny, because horizontal gene transfer has allowed their evolution in function to the legume host fitness.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"47 1-2","pages":"43-60"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26385660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular mechanism for pathogenicity of Salmonella sp]. 沙门氏菌致病性的分子机制

Revista latinoamericana de microbiologia Pub Date : 2005-01-01

Inda Marcela Figueroa Ochoa, Antonio Verdugo Rodríguez

引用次数: 0

Neonatal candidiasis in Venezuela: clinical and epidemiological aspects. 委内瑞拉新生儿念珠菌病:临床和流行病学方面。

Revista latinoamericana de microbiologia Pub Date : 2005-01-01

Claudia Hartung de Capriles, Sofía Mata-Essayag, Amparo Azpiróz, Adriana Ponente, Sylvia Magaldi, Celina Pérez, Arantza Roselló, María Teresa Colella, Jensi Machuca

{"title":"Neonatal candidiasis in Venezuela: clinical and epidemiological aspects.","authors":"Claudia Hartung de Capriles, Sofía Mata-Essayag, Amparo Azpiróz, Adriana Ponente, Sylvia Magaldi, Celina Pérez, Arantza Roselló, María Teresa Colella, Jensi Machuca","doi":"","DOIUrl":"","url":null,"abstract":"A prospective study of the clinical and epidemiological aspects of Candida spp. sepsis was performed to assess the frequency, etiology, and risk factors in the neonatology service of the Pediatrics Hospital \"Dr. Elías Toro\" (2002-2003). Forty four out of 128 neonatal intensive care patients, with clinical sepsis and suspected fungal etiology, were chosen randomly for this study. Infant blood, urine, gastrointestinal tract, oral and skin samples were cultured. Samples were also taken from health care worker hands and the environment. The antifungal susceptibility patterns of the isolates were evaluated. The prevalence of Candida spp. from the clinical samples was: C. albicans (72.06%), C. parapsilosis (13.24%), C. tropicalis (10.29 %), C. guilliermondii (2.94%), and C. glabrata (1.47%). Due to the similarity of the susceptibility pattern of some isolates from infants and health care workers, we speculate a horizontal nosocomial infection. Statistical analysis revealed the following significant risk factors associated with Candida spp. isolation: prolonged hospitalization (p < 0.05), missing prenatal birth control (p < 0.05), and parenteral nutrition (p < 0.05). Blood cultures were all negative for bacteria and only 2.90% were positive for Candida spp. All 44 neonates receiving empirical therapy with amphotericin B (0.5-1.0 mg/k/day) evolved satisfactorily.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"47 1-2","pages":"11-20"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serotypes of 286 group B streptococci isolated from asymptomatic carriers and invasive disease cases in Mexico. 墨西哥无症状携带者和侵袭性疾病病例分离286株B群链球菌血清型分析

Revista latinoamericana de microbiologia Pub Date : 2005-01-01

Gerardo C Palacios, María N González, Magdalena Beltrán, José L Arredondo, Javier Torres, Fortino Solórzano

{"title":"Serotypes of 286 group B streptococci isolated from asymptomatic carriers and invasive disease cases in Mexico.","authors":"Gerardo C Palacios, María N González, Magdalena Beltrán, José L Arredondo, Javier Torres, Fortino Solórzano","doi":"","DOIUrl":"","url":null,"abstract":"Background: Group B Streptococcus (GBS) remains as a leading cause of neonatal sepsis and meningitis in developed countries, where type III is the most common serotype. Although GBS is considered an uncommon cause of perinatal pathology in Mexico, a vaginal colonization rate of 14% in pregnant women and a neonatal infection rate of 1/1500 live births have been reported. The aim of this study was to determine the serotype distribution in a collection of 286 GBS strains isolated in Mexico from asymptomatic carriers and in adult and neonatal invasive disease cases.Methods: The collection included GBS strains isolated between January 1988 and April 1998 at the Instituto Nacional de Perinatologia and Hospital de Pediatria in Mexico City. GBS and serotype were confirmed by latex agglutination.Results: Most strains were isolated from asymptomatic carriers (66%). 30% were invasive isolates, and 10% of them were from neonates. 48.6% were type I, 32.9% type III, 14% type II, and 4% were non-typeable.Conclusion: Serotype I is predominant in Mexico but participation of serotype III is increasing, and a decrease of non-typeable isolates was detected.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"47 1-2","pages":"21-4"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grazing of heterotrophic dinoflagellate Noctiluca scintillans (Mcartney) Kofoid on Gymnodinium catenatum Graham. 异养双鞭毛夜行虫(Noctiluca scintillans (mccarney) Kofoid)对裸子草的放牧。

Revista latinoamericana de microbiologia Pub Date : 2005-01-01

Rosalba Alonso Rodríguez, José Luis Ochoa, Manuel Uribe Alcocer

引用次数: 0

Evaluation of the DIRAMIC system for detection of urinary tract infections and for Escherichia coli identification. DIRAMIC系统用于尿路感染检测和大肠杆菌鉴定的评价。

Revista latinoamericana de microbiologia Pub Date : 2004-07-01

Fernando Travieso Ruiz, Gloria Roura Carmona, Cheyla Romay Penabad, Rolando Contreras Alarcón

{"title":"Evaluation of the DIRAMIC system for detection of urinary tract infections and for Escherichia coli identification.","authors":"Fernando Travieso Ruiz, Gloria Roura Carmona, Cheyla Romay Penabad, Rolando Contreras Alarcón","doi":"","DOIUrl":"","url":null,"abstract":"The use of the DIRAMIC system for the detection of urinary tract infections (UTI) and the possibility to identify Escherichia coli in the same culture media was evaluated. The results from DIRAMIC detection system were compared to counts of colony forming units per milliliter (CFU/ml) of urine inoculated in CLED Medium; 884 urine specimens were processed taking > or =10(4) CFU/ml as criteria of positive urine culture counts. For E. coli identification, substrates for the determination of beta-glucuronidase and tryptophanase were incorporated to the culture medium and named DETID-Ec. Outputs were compared to those from API RAPIDEC-ur strips. The DIRAMIC system can detect UTI, with a sensitivity and specificity of 82.25 and 94.49%, respectively. It was possible to identify E. coli during detection with 87.50% of sensitivity and 95.96% of specificity. The small volumes of culture medium used in the DIRAMIC system as well as the short times for the detection make the system a rapid and economical method for screening UTI. Furthermore, by using DETID-Ec culture medium the time and the number of biochemical tests necessary for the E. coli identification are lowered.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"46 3-4","pages":"67-71"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary characterization of microbial communities in high altitude wetlands of northwestern Argentina by determining terminal restriction fragment length polymorphisms. 阿根廷西北部高海拔湿地微生物群落的末端限制性片段长度多态性初步表征。

Revista latinoamericana de microbiologia Pub Date : 2004-07-01

Marcela Ferrero, María E Farías, Faustino Siñeriz

{"title":"Preliminary characterization of microbial communities in high altitude wetlands of northwestern Argentina by determining terminal restriction fragment length polymorphisms.","authors":"Marcela Ferrero, María E Farías, Faustino Siñeriz","doi":"","DOIUrl":"","url":null,"abstract":"Laguna de Pozuelos is an extensive wetland in Morthwestern Argentina at 3,600 m above sea level in the Argentinean Andes. The principal lake, placed in the central depression of endorheic basin, is rich in minerals like Cu, As, Fe, etc. It collects water from underground courses and from two main tributaries, namely Santa Catalina River to the north and Cincel River to the south. Following the dry and rainy seasons, the surface of the lake is subject to an annual contraction-expansion cycle, with increasing of salinity during evaporation period. Prokaryotes inhabitants these particular environments have been not described and a few of such places have been surveyed for microbial diversity studies. To systematically explore the underlying communities of Bacteria from the water lake of Laguna de Pozuelos wetland and Cincel River, bacterial 16S rRNA genes (rDNAs) were PCR amplified and analyzed by terminal restriction fragment length polymorphism (T-RFLP) analysis. Analysis of the microbial community with T-RFLP identified a minimum of 19 operational taxonomic units (OTU). T-RF patterns derived from multiple-enzyme digestion with RsaI, HaeIII and HhaI were analyzed in order to provide a preliminary picture of the relative diversity of this complex microbial community. By the combined use of the three restriction endonucleases bacterial populations of this particular place were identified.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"46 3-4","pages":"72-80"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave irradiation for shortening the processing time of samples of flagellated bacteria for scanning electron microscopy. 微波辐照缩短扫描电镜鞭毛菌样品处理时间。

Revista latinoamericana de microbiologia Pub Date : 2004-07-01

Francisco Hernández-Chavarría

{"title":"Microwave irradiation for shortening the processing time of samples of flagellated bacteria for scanning electron microscopy.","authors":"Francisco Hernández-Chavarría","doi":"","DOIUrl":"","url":null,"abstract":"Microwave irradiation (MWI) has been applied to the development of rapid methods to process biological samples for scanning electron microscopy (SEM). In this paper we propose two simple and quick techniques for processing bacteria (Proteus mirabilis and Vibrio mimicus) for SEM using MWI. In the simplest methodology, the bacteria were placed on a cover-glass, air-dried, and submitted to conductivity stain. The reagent used for the conductivity stain was the mordant of a light microscopy staining method (10 ml of 5% carbolic acid solution, 2 g of tannic acid, and 10 ml of saturated aluminum sulfate 12-H2O). In the second method the samples were double fixed (glutaraldehyde and then osmium), submitted to conductivity stain, dehydrated through a series of ethanol solutions of increasing concentration, treated with hexamethyldisilazine (HMDS), and dried at 35 degrees C for 5 minutes. In both methods the steps from fixation to treatment with HMDS were done under MWI for 2 minutes in an ice-water bath, in order to dissipate the heat generated by the MWI. Although both techniques preserve bacterial morphology adequately, the latter, technique showed the best preservation, including the appearance of flagella, and that process was completed in less than 2 hours at temperatures of MWI between 4 to 5 degrees C.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"46 3-4","pages":"81-4"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0