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Microbiological determinations of some vegetables from the Xochimilco zone in Mexico City, Mexico. 墨西哥墨西哥城 Xochimilco 地区一些蔬菜的微生物测定。
Revista latinoamericana de microbiologia Pub Date : 2002-01-01
Rolando García-Gómez, José Chávez-Espinosa, Adriana Mejía-Chávez, Carmen Duránde-Bazúa
{"title":"Microbiological determinations of some vegetables from the Xochimilco zone in Mexico City, Mexico.","authors":"Rolando García-Gómez, José Chávez-Espinosa, Adriana Mejía-Chávez, Carmen Duránde-Bazúa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Vegetables intake is widely recommended because of its high content of vitamins, minerals and fiber. However, the irrigation of these vegetables, using wastewaters that have received inadequate treatment often carries unseen microbial pollution that becomes a high risk potential for humans. In the present research, two of the most consumed fresh vegetables cultivated in Mexico City were analyzed, lettuce (Lactuca sativa) and Mexican coriander (Eryngium foetidum). These vegetables are commonly consumed raw. The vegetable choice and the disinfection's method were carried out by the application of two tests to two hundred people in an aleatory form. Similarly, vegetable sampling was carried out by means of a random sampling from the cultivated areas in a chosen \"chinampa\" (from Náhuatl or Aztec, chinamitl, bulrush or cattail stalks lattice for hydroponics cultivation). Vegetable samples were transferred, in dark plastic bags and in cool boxes at 4 +/- 1.5 degrees C, to the laboratory. Microbiological analysis for Salmonella typhi, mesophilic microorganisms, and fecal coliforms were done according to the \"NOM-093SSA1-1994\" (Mexico). Results obtained demonstrated that samples treated with the most preferred disinfectant, a colloidal silver based one, had a partial elimination of pathogenic microorganisms found in both vegetables lettuce (Lactuca sativa) and coriander (Eryngium foetidum) samples (mesophyllic microorganisms from 200,000 to 96,500 UFC/g and from 175,000 to 125,000 UFC/g and fecal coliforms from 75 to 0.43 NMP/g and from 150 to 2.10 NMP/g, respectively). Salmonella typhi for all samples gave a positive result. Therefore, it was recommended to the cultivators of the Xochimilco (Náhuatl or Aztec name that means \"place where flowers bloom\") zone, either stop using contaminated water for irrigation or to use more efficient methods in order to eliminate pathogenic microorganisms, such as diluted chlorine solutions made with commercial cotton clothing bleachers.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"44 1","pages":"24-30"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26326844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Control of amylase production and growth characteristics of Aspergillus ochraceus. 赭曲霉淀粉酶产率及生长特性的控制。
Revista latinoamericana de microbiologia Pub Date : 2002-01-01
Ely Nahas, Mirela M Waldemarin
{"title":"Control of amylase production and growth characteristics of Aspergillus ochraceus.","authors":"Ely Nahas,&nbsp;Mirela M Waldemarin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The growth and the extracellular amylase production by Aspergillus ochraceus were studied in a stationary culture medium. Maximum growth rate of this fungus was found after 5 days of incubation at 30 degrees C, but maximum amylase production was obtained after 2 days. The highest amylase production were attained with lactose, maltose, xylose and starch as carbon sources. The extracellular amylase production and mycelial growth were influenced by the concentration of starch. Other carbohydrates supported growth but did not induce amylase synthesis and glucose repressed it, indicating catabolite repression in this microorganism. The presence of both mechanisms of induction and repression suggests that at least these multiple forms of regulation are present in A. ochraceus. Of the nitrogen sources tested, casaminoacids, ammonium nitrate and sodium nitrate stimulated the highest yield of amylase. Optimal amylase production was obtained at pH 5.0, but enzyme activity was found only in the 4.0-6.0 pH range. These results were probably due to the inhibitory effect of NH4(+)-N in the culture medium.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"44 1","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26326840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical significance of the redefinition of the agent of amoebiasis. 阿米巴病病原重新定义的临床意义。
Revista latinoamericana de microbiologia Pub Date : 2001-10-01
R Lucas, J A Upcroft
{"title":"Clinical significance of the redefinition of the agent of amoebiasis.","authors":"R Lucas,&nbsp;J A Upcroft","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Entamoeba histolytica is the pathogenic species of Entamoeba that causes amoebic dysentery and other invasive disease. The morphologically similar species, E. dispar, is non-pathogenic and accounts for about 90% of the previously estimated 500 million E. histolytica infections world-wide. Because of the recent redefinition of E. histolytica and E. dispar, and the limited number of drugs available to treat amoebiasis, a new approach to treatment of individuals carrying these parasites is necessary. A meeting of eminent scientists has recently agreed that on no account should prophylaxis against amoebiasis be given, and no treatment without symptoms should be administered. The expense of treating asymptomatic individuals, both monetary and at the risk of over-use of precious drugs, does not appear to be justified. It would seem wise that we preserve currently effective anti-amoebic drugs and avoid the development of drug-resistant E. histolytica.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"43 4","pages":"183-7"},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26326839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León. 新墨西哥州蒙特雷郊区马的伯氏疏螺旋体抗体检测León。
Revista latinoamericana de microbiologia Pub Date : 2001-10-01
J A Salinas-Mélendez, S Galván de la Garza, V M Riojas-Valdés, A Wong González, R Avalos-Ramírez
{"title":"Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León.","authors":"J A Salinas-Mélendez,&nbsp;S Galván de la Garza,&nbsp;V M Riojas-Valdés,&nbsp;A Wong González,&nbsp;R Avalos-Ramírez","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of the present study was to determine the presence of Borrelia burgdorferi antibodies in horses from the metropolitan area of Monterrey, Nuevo León, México. Blood serum was obtained from a total of 100 horses residing at different counties in the area. From each animal data was obtained on age, sex, county of residence, presence of ectoparasites and clinical signs. All sera samples were analyzed by indirect immunofluoresence and the sera that resulted positive to this test was analyzed by Western blot. The serological test yielded 34 positive sera at 1:64 dilution, and from them 6 were positive at 1:128 dilution, 3 at 1:256, and only one at 1:512. Confirmation of the infection by Western blot was obtained only in the sample positive at the 1:512 dilution. These results shown a low frequency of seropositivity to B. burgdorferi of the horses in the area, confirming previous studies indicating that in northeast Mexico Lyme disease is present in different animal species.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"43 4","pages":"161-4"},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytotoxin production in phytopathogenic and entomopathogenic Serratia marcescens. 植物致病性和昆虫致病性 Serratia marcescens 产生的细胞毒素。
Revista latinoamericana de microbiologia Pub Date : 2001-10-01
M M Escobar, G V Carbonell, L O Beriam, W J Siqueira, T Yano
{"title":"Cytotoxin production in phytopathogenic and entomopathogenic Serratia marcescens.","authors":"M M Escobar, G V Carbonell, L O Beriam, W J Siqueira, T Yano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this work, culture filtrates of entomopathogenic and phytopathogenic Serratia marcescens strains induced cytotoxic effects on CHO, Vero and HEp-2 cell lines. Morphological changes on sensitive cells were characterized by cell rounding and detachment as soon as 30 min of incubation, culminating in cell death after 24 h. The cytotoxic effect was completely neutralized by specific antiserum indicating that occur antigenic similarity among cytotoxins produced by these strains. The toxicity assays on plants showed that the culture supernatants did not provoke any visible morphological change and did not affect their growth. By contrast, the plants treated with bacterial suspension showed disease symptom, such as shriveling and decay of stores bulbus in onion and lettuce plantlets. In conclusion, this study show that phytopathogenic and entomopathogenic S. marcescens may produce a cytototoxin similar to that produced by clinical isolates and it is toxic to different mammalian cell lines. These results are especially important for studies involving this bacterium as biological control agent.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"43 4","pages":"165-70"},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hydrogen peroxide production and resistance to nonoxinol-9 in Lactobacillus spp. isolated from the vagina of reproductive-age women. 从育龄妇女阴道分离的乳杆菌产生过氧化氢和对壬醇-9的抗性。
Revista latinoamericana de microbiologia Pub Date : 2001-10-01
M Angeles-López, E García-Cano Ramos, C Aquino Santiago
{"title":"Hydrogen peroxide production and resistance to nonoxinol-9 in Lactobacillus spp. isolated from the vagina of reproductive-age women.","authors":"M Angeles-López,&nbsp;E García-Cano Ramos,&nbsp;C Aquino Santiago","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Lactic acid production is considered to be the major protection mechanism of lactobacilli against vaginal infections due to genital pathogens. However, some species of Lactobacillus are also hydrogen peroxide-producers. Women, who usually use intrauterine dispositive (IUD) and spermicides such as nonoxinol-9 (N-9) as contraceptive methods, increase the risk of acquiring an urinary tract infection and a bacterial vaginosis; some studies have demonstrated that these compounds alter the normal vaginal biota. It is known that they inhibit lactobacilli in vitro at concentrations of 0.1% to 1% and that they do not have an effect on the growth of Escherichia coli. It is probable that the presence of nonoxinol-9 affects the ecological balance of the vagina by inhibiting the protector lactobacilli. In this study, we identified Lactobacillus acidophilus, L. brevis, L. crispatus, L. fermentii and L. jensenii as the species most frequently isolated from women. Seventy-one hydrogen peroxide-producer strains and 48 strains resistant to the inhibitory effect of nonoxinol-9 were detected. L. brevis showed the highest number of resistant strains.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"43 4","pages":"171-6"},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epidemiology of sexually transmitted diseases in Río Cuarto, Argentina. 阿根廷库托Río性传播疾病的流行病学。
Revista latinoamericana de microbiologia Pub Date : 2001-10-01
M C Pájaro, I L Barberis, S Godino, L Pascual, M Agüero
{"title":"Epidemiology of sexually transmitted diseases in Río Cuarto, Argentina.","authors":"M C Pájaro,&nbsp;I L Barberis,&nbsp;S Godino,&nbsp;L Pascual,&nbsp;M Agüero","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Sexually transmitted diseases (STD) are a group of transmittable diseases acquired fundamentally through sexual contact. STD are a social problem resulting from demographic explosion and changes in sexual conduct, which affects teenagers and adults of all socioeconomic strata. The goal of this work was to establish the actual state of the different STD within the studied population. Samples of vaginal fluids, endocervical materials and urethral exudates taken from 2,630 patients during five years were processed. 1,341 samples tested positive to one or more of the microorganisms, 1,099 corresponding to female patients and 242 to male patients. The microorganisms found in women were: Gardnerella vaginalis (39.1%), Candida albicans (21.3%), Trichomonas vaginalis (16.8%), Chlamydia trachomatis (11.5%); Neisseria gonorrhoeae (3.4%), Mycoplasma hominis (2.6%); Ureaplasma urealyticum (4.1%) and Treponema pallidum (1.6%). Associations were: Gardnerella vaginalis with Trichomonas vaginalis (6%), Gardnerella vaginalis with Candida albicans (5.1%); Trichomonas vaginalis with Neisseria gonorrhoeae (2.2%) and Gardnerella vaginalis with Chlamydia trachomatis (2.1%). In men, gonococcic urethritis represented 37.8%, non-gonococcic urethritis 55.4% and Treponema pallidum 6.8%. A decrease in syphilis, gonococcic urethritis and gonococcic cervicitis was observed, increasing the prevalence of non-gonococcic urethritis and cervicitis. This study showed that in our environment the actual tendency of STD is still high.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"43 4","pages":"157-60"},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytomegalovirus infection in patients with solid-organ transplant. Recently reviewed immunologic response and pathogenicity mechanisms. 实体器官移植患者巨细胞病毒感染。最近综述了免疫反应和致病性机制。
Revista latinoamericana de microbiologia Pub Date : 2001-10-01
R Caltenco-Serrano, J L Sánchez-Huerta, R Vargas-Jiménez, R S Rodríguez-Suárez, D Gómez-Barreto
{"title":"Cytomegalovirus infection in patients with solid-organ transplant. Recently reviewed immunologic response and pathogenicity mechanisms.","authors":"R Caltenco-Serrano,&nbsp;J L Sánchez-Huerta,&nbsp;R Vargas-Jiménez,&nbsp;R S Rodríguez-Suárez,&nbsp;D Gómez-Barreto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cytomegalovirus (CMV) infection in immunocompromised host is an important cause of morbidity and mortality. The protective immunity against the virus is both humoral and cellular. Immunologic mechanisms in rejection as in the immune response against CMV infection are similar but there is difficult to separe as histologic and clinically independent events. At least eight different genes of CMV are homologous to human proteins related to the immune response. The potential role of these genes with homology to human genes can be at different levels. The relevance that immunodominant antigens have on the natural control of CMV infection, suggests that the future design of a vaccine directed to protecting from disease those susceptible to primary infection, in an immunocompromised state, should involve a combination of antigens that include pp65, IE1-exon 4 and gB as a recombinant proteins.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"43 4","pages":"177-82"},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26326838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunological factors governing resistance and susceptibility of mice to Leishmania major infection. 控制小鼠对利什曼原虫大感染的抗性和易感性的免疫因素。
Revista latinoamericana de microbiologia Pub Date : 2001-07-01
F Aguilar-Torrentera, Y Carlier
{"title":"Immunological factors governing resistance and susceptibility of mice to Leishmania major infection.","authors":"F Aguilar-Torrentera,&nbsp;Y Carlier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Infection with Leishmania sp. is particularly suitable for the study of immunoregulatory mechanisms associated with host susceptibility or resistance. The clinical spectrum of this infection results from parasite virulence factors and host immune responses, some of which acting in a host protective manner while others exacerbate the disease. In the mouse model, factors governing resistance to Leishmania major infection mainly depends on the IFN-gamma activation of the leishmanicidal function of macrophages, and the Fas/ FasL-dependent T-cell cytotoxicity against infected macrophages. On the other hand, the immunological factors of susceptibility involve: I) the early upregulation of IL-4 production induced by the LACK antigen, II) the upregulation of IL-2 production, III) the high production of TGF-beta as macrophage deactivating factor, and IV) the production of IL-10 by the L. major infected macrophages, inhibited their microbicidal activity.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"43 3","pages":"135-42"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26326941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surface morphology and characteristics of hemocytes of Biomphalaria glabrata (Pulmonata: Planorbidae) from two geographic sources. 两个地理来源的光斑生物phalaria (Pulmonata: planorbida)的表面形态和血细胞特征。
Revista latinoamericana de microbiologia Pub Date : 2001-07-01
V Delgado, E E Barrios, A Bujanda, W Araque
{"title":"Surface morphology and characteristics of hemocytes of Biomphalaria glabrata (Pulmonata: Planorbidae) from two geographic sources.","authors":"V Delgado,&nbsp;E E Barrios,&nbsp;A Bujanda,&nbsp;W Araque","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Biomphalaria glabrata is a fresh water snail of medical importance since it is the intermediate host of the trematode Schistosoma mansoni. The internal defense system of mollusks is mostly represented by circulating elements of the hemolymph (hemocytes). The infectivity of S. mansoni to B. glabrata is determined by genetic factors and evolutionary adaptations. In the present work factors about the parasite/snail relationship were evaluated, especially those related to the morphology and characteristics of the surface of cells present in the hemolymph of two strains of B. glabrata: a strain with high susceptibility to S. mansoni (Puerto Rico, PR) and a strain with medium susceptibility (Caripe, Ca). Hemolymph was collected by cephalopodal puncture; a total and a differential count of hemocytes were done in dyed preparations and through scanning electron microscopy (SEM). Results from both strains show a high quantitative variability of the total hemocyte count. Hemocytes dyed with May-Grünwald Giemsa showed cells with a basophilic nucleus predominantly in PR (61.7%), dense and strongly basophilic in Ca (71.2%) with significant differences between them. Through SEM round cells with a corrugated surface were observed (6-10microm), hemocytes with an irregular spongy surface (12microm), others with many projections (16microm) and cells not reported in similar to erythrocytes (21microm). Hemocytic cells from both strains confirmed cytoadherence and encapsulation were confirmed in the hemocytic cells from both strains, while no differences associated to the susceptibility of the strains were observed after 2 h of parasite-hemocyte incubation.</p>","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"43 3","pages":"114-8"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26385572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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