Revista latinoamericana de microbiologia最新文献_第9页

Rat DNA polymerase beta substitutes the repairing activity of DNA polymerase I in the lethal effect of UV light. 大鼠DNA聚合酶β替代DNA聚合酶I的修复活性参与紫外线致死作用。

Revista latinoamericana de microbiologia Pub Date : 2002-04-01

R Hernández-Escamilla, J M Espinosa-Lara, J D Quintana-Hau, S Uribe-Luna, P Loyola-Abitia, J C Santiago-Hernández, R Maldonado-Rodríguez

{"title":"Rat DNA polymerase beta substitutes the repairing activity of DNA polymerase I in the lethal effect of UV light.","authors":"R Hernández-Escamilla, J M Espinosa-Lara, J D Quintana-Hau, S Uribe-Luna, P Loyola-Abitia, J C Santiago-Hernández, R Maldonado-Rodríguez","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this work was to search if the rat DNA polymerase beta can substitute the capability of DNA polymerase I to repair damage caused by the UV light in Escherichia coli. The oriC origin of replication from p beta 5 was replaced by the rep origin from pSC101 and named p beta 6. The presence of pol beta in the new construct was verified by PCR. E. coli polA-1 (WP6) was transformed with p beta 6. A protein with size similar to DNA Pol beta (40 kDa) was shown in the cell free extracts carrying pbeta5. In WP6/p beta 6 cell free extracts a slightly smaller protein was observed instead of the 40 kDa. DNA Pol beta was revealed by western analysis, with polyclonal antibodies, in strains with p beta 5. Yet, it was not detected in the western from WP6/p beta 6. A moderate change in UV resistance was observed in strains carrying p beta 5. However, in polAl carrying p beta 6 (WP6/p beta 6), irradiated with 60-90 J/m2 of UV light, the viability was increased by more than four orders of magnitude, when compared with the polA1 (WP6) strain, reaching approximately the same UV resistance as the strains with DNA polymerase I. The results suggests that probably Pol beta is rapidly degraded in the cell free extracts from WP6/p beta 6 and, it repairs the lethal effect of the UV light in E. coli.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"44 2","pages":"58-64"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26329141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Susceptibility to 5-fluorocytosine, miconazole and amphotericin B of Candida albicans strains isolated from the throat of non-AIDS patients. 非艾滋病患者喉部白色念珠菌对5-氟胞嘧啶、咪康唑和两性霉素B的敏感性。

Revista latinoamericana de microbiologia Pub Date : 2002-04-01

Gloria Luz Paniagua, Eric Monroy, Erasmo Negrete, Sergio Vaca

{"title":"Susceptibility to 5-fluorocytosine, miconazole and amphotericin B of Candida albicans strains isolated from the throat of non-AIDS patients.","authors":"Gloria Luz Paniagua, Eric Monroy, Erasmo Negrete, Sergio Vaca","doi":"","DOIUrl":"","url":null,"abstract":"Eighty Candida albicans strains, isolated from throat of patients at the Universitary Clinic of the Faculty of Superior Studies Iztacala of the National Autonomous University of Mexico, were analyzed. They were identified by microscopic and colony morphologies, germ tube test, and by auxanogram and zimogram. Minimal inhibitory concentrations (MIC) of 5-fluorocytosine, miconazole and amphotericin B were determined by microtiter broth dilution. MIC frequency distribution of 5-fluorocytosine showed a single peak (0.25-8.0 microg/ml), with 65% susceptible strains (MIC < or = 1.0 < or =g/ml) and 35% intermediate susceptible strains (MIC = 1.1-8 microg/ml). MIC frequency distribution of miconazole was threemodal with 6.25% susceptible (MIC = 1.562 microg/ml), 48.75% intermediate susceptible (MIC = 3.125-12.5 microg/ml), and 45% resistant (MIC = 25-50 microg/ml) strains. All strains were susceptible to amphotericin B (MIC= 0.0156-0.125 microg/m). These results shows that amphotericin B was the more active antimycotic, followed by 5-fluorocytosine, against the strains analyzed, and that miconazole was the less effective one.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"44 2","pages":"65-8"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26329142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between free radicals produced by entamoeba histolytica and its proteases complex activity. 溶组织内阿米巴产生的自由基与其蛋白酶复合物活性的关系。

Revista latinoamericana de microbiologia Pub Date : 2002-04-01

M P Crisóstomo-Vázquez, M P Cervantes-Cervantes, E Jiménez-Cardoso, J L Muñoz-Sánchez

{"title":"Relationship between free radicals produced by entamoeba histolytica and its proteases complex activity.","authors":"M P Crisóstomo-Vázquez, M P Cervantes-Cervantes, E Jiménez-Cardoso, J L Muñoz-Sánchez","doi":"","DOIUrl":"","url":null,"abstract":"Entamoeba histolytica is a parasite which causes health problems and there has been many approaches to know what is the factor causing its pathogenicity. In the present work, we assayed if the production of free radicals by the amoeba, has a relationship with the proteases activity. When we test the DMSO action (free radicals quenching activity) the specific activity of the proteases complex of the parasite were affected also. At 33.3% (V/V) concentration of DMSO it was present a maximal decrease of the initial activity (about 46% decrease), for to a higher concentrations existing a trend to recuperate the original activity, suggesting that the free radicals are an important factor for the hydrolysis grade of the protein substrate. All the differences except those between 46.7 and 66.6%, were significantly different compared with the control without any addition. The effects of Probucol and Probucol plus DMSO, compared to those caused by Metronidazol (MZ). We can observe that the quenchers caused a decrease on proteases activity similar to that of MZ (which is an antiparasite drug) and it was of c.a. 58% of activity decrease. These data suggest that the action of both, free radicals and proteases complex of Entamoeba histolytica, can account for the pathogenicity of the parasite.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"44 2","pages":"79-82"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26329145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Some biological features of mollicutes. 分子的一些生物学特性。

Revista latinoamericana de microbiologia Pub Date : 2002-04-01

José Antonio Rivera-Tapia, María Lilia Cedillo-Ramírez, Constantino Gil Juárez

引用次数: 0

Serologic survey in animals of 'Q' fever in Nuevo Leon. 新莱昂州Q热动物血清学调查。

Revista latinoamericana de microbiologia Pub Date : 2002-04-01

J A Salinas-Melédez, R Avalos-Ramírez, V Riojas-Valdez, J Kawas-Garza, H Fimbres-Durazo, G Hernández-Vidal

引用次数: 0

Bioethics, bioweapons and the microbiologist. 生物伦理学，生物武器和微生物学家。

Revista latinoamericana de microbiologia Pub Date : 2002-01-01

Fernando Anaya-Velázquez

{"title":"Bioethics, bioweapons and the microbiologist.","authors":"Fernando Anaya-Velázquez","doi":"","DOIUrl":"","url":null,"abstract":"The analysis of behavior of man in the field of biology is carried out through bioethics, considered the science of the survival. In the microbiology, there are numerous discoveries related with pathogenic microorganisms, including those that can be used as weapons in a biological war or in an attack considered bioterrorism. The scientist involved in microbiology can participate with his knowledge in the development and improvement of bioweapons, however from the point of view of bioethics it is not acceptable that he works in an investigation related with these topics, because the defense research can evolve in offensive one. The war is an antisurvival activity, therefore it is not acceptable. In the same way, the biological weapons composed with virus, fungi or alive bacteria, or with toxins from them, neither they are morally accepted. After the terrorist attacks with anthrax in the United States in 2001, the world scientific community in the field of microbiology should show against the use of the microorganisms like bioweapons, at the time of promoting the idea that the responsible use for the microorganisms is a moral imperative for all microbiologists around the world, since the biological weapons are a threat for the human life.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"44 1","pages":"38-45"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short term effects of Glomus claroideum and Azospirillum brasilense on growth and root acid phosphatase activity of Carica papaya L. under phosphorus stress. 磷胁迫下球囊菌和巴西氮螺旋菌对番木瓜生长和根系酸性磷酸酶活性的短期影响

Revista latinoamericana de microbiologia Pub Date : 2002-01-01

Alejandro Alarcón, Frederick T Davies, Johnatan N Egilla, Theodore C Fox, Arturo A Estrada-Luna, Ronald Ferrera-Cerrato

{"title":"Short term effects of Glomus claroideum and Azospirillum brasilense on growth and root acid phosphatase activity of Carica papaya L. under phosphorus stress.","authors":"Alejandro Alarcón, Frederick T Davies, Johnatan N Egilla, Theodore C Fox, Arturo A Estrada-Luna, Ronald Ferrera-Cerrato","doi":"","DOIUrl":"","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are able to increase root enzymatic activity of acid and alkaline phosphatases. However, the role of AMF on phosphatase activity has not been reported in papaya (Carica papaya L.), which is frequently established at places with soil phosphorus (P) deficiencies. The goals of this research were to determine the effect of Glomus claroideum (Gc), and plant growth promoting rhizobacterium Azospirillum brasilense strain VS7 [Ab]) on root phosphatase activity and seedling growth of Carica papaya L. cv. Red Maradol under low P conditions. There were four treatments-colonization with: 1) Gc, 2) Ab, 3) Gc+Ab, and 4) non-inoculated seedlings. Plants were established in a coarse sand:sandy loam substrate under P-limitation (11 microg P ml(-1)), supplied with a modified Long Ashton Nutrient Solution. Seedling growth was severely reduced by low P. Gc+Ab inoculated plants had greater total dry matter and leaf area than non-colonized plants. Gc-inoculated plants had greater leaf area than non-colonized plants. Treatments did not differ in leaf area ratio, specific leaf area and, total chlorophyll content. There was a non-significant effect on stem relative growth rate with Gc and Gc+Ab plants. Mycorrhizal colonization enhanced the bacterial population 3.4-fold in the Gc+Ab treatment compared with the population quantified in Ab treatment. Soluble and extractable root acid phosphatase activity (RAPA) was higher in Gc inoculated plants. We discussed on the possible relation among both inoculated microorganisms and also with the P-limitation which plants were established.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"44 1","pages":"31-7"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26326845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osmoregulated TAQ polymerase gene expression in Escherichia coli. 渗透调节TAQ聚合酶基因在大肠杆菌中的表达。

Revista latinoamericana de microbiologia Pub Date : 2002-01-01

Yeosvany Cabrera Artiles, Duniesky Martínez García, Enrique R Pérez Cruz, Gabriel J Márquez Perera, Manuel Luis Feble

引用次数: 0

Virulence factors of A. caviae strains isolated from acute diarrheic disease in Cuba. 古巴急性腹泻病分离的鼠腹曲霉毒力因子分析。

Revista latinoamericana de microbiologia Pub Date : 2002-01-01

Graciela Castro Escarpulli, Dayrema Peña del Barrio, N Castañeda, Ailec García Azcuaga, Luis Morier Dias, Maria Guadalupe Aguilera-Arreola, Laura Bravo Farias

引用次数: 0

Effects of the bacteriocin PsVP-10 produced by Pseudomonas sp. on sensitive bacterial strains. 假单胞菌产生的细菌素PsVP-10对敏感菌株的影响。

Revista latinoamericana de microbiologia Pub Date : 2002-01-01

Carlos Padilla, Olga Lobos, Pedro Brevis, Paulina Abaca, Elizabeth Hubert

{"title":"Effects of the bacteriocin PsVP-10 produced by Pseudomonas sp. on sensitive bacterial strains.","authors":"Carlos Padilla, Olga Lobos, Pedro Brevis, Paulina Abaca, Elizabeth Hubert","doi":"","DOIUrl":"","url":null,"abstract":"The bacteriocin PsVP-10 is a 2.6 Kda peptide which was isolated and purified from Pseudomonas sp. This bacteriocin possesses lethal activity over Enterococcus faecalis, Salmonella typhimurium and Shigella flexneri. The experimental assays showed that the bacteriocin is able to be adsorbed by all cells of these bacterial species and also by their isolated cell walls. It was observed that the resistant mutants and their respective cell walls are unable to adsorb the bacteriocin. Assays performed with spheroplasts obtained from sensitive bacterial species and their resistant mutants show a rapid lethal effect of the bacteriocin PsVP-10. This results indicated furthermore, it is also shown that the optimal pH and temperature for the adsorption were 7.2 and 37 degrees C, respectively. The study carried out with organic solvents like methanol, ethanol, isopropanol and the detergents sodium dodecyl sulfate and triton X-100 showed a moderate inhibition of the bacteriocin lethal action for the Gram negative cells. The enzymes lysozime, protease XIV and trypsine type III-S did not present any effect over the adsorption capacity of the bacteriocin with any of the bacterial species studied.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"44 1","pages":"19-23"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26326843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0