Revista latinoamericana de microbiologia最新文献

Pathogenic and opportunistic gram-negative bacteria in soil, leachate and air in San Nicolás landfill at Aguascalientes, Mexico. 墨西哥阿瓜斯卡连特斯圣Nicolás垃圾填埋场土壤、渗滤液和空气中的致病性和机会性革兰氏阴性菌。

Revista latinoamericana de microbiologia Pub Date : 2007-01-01

Francisco J Flores-Tena, Alma L Guerrero-Barrera, Francisco J Avelar-González, E Marcela Ramírez-López, M Consolación Martínez-Saldaña

{"title":"Pathogenic and opportunistic gram-negative bacteria in soil, leachate and air in San Nicolás landfill at Aguascalientes, Mexico.","authors":"Francisco J Flores-Tena, Alma L Guerrero-Barrera, Francisco J Avelar-González, E Marcela Ramírez-López, M Consolación Martínez-Saldaña","doi":"","DOIUrl":"","url":null,"abstract":"The occurrence of Gram-negative pathogenic and opportunistic species, was studied for two years on air. soil and leachate from the San Nicolás Landfill. Aguascalientes, Mexico. For soil and leachate four samplings were done, two during the dry season and two during the rainy season. For soil there were 15 sampling points, the leachate samples were taken on the leachate tank. For air, twelve sampling were done in three points of the landfill. Twenty pathogenic and/or opportunistic bacteria were identified from air, twenty from soil and eleven from leachate. Most of them were enteric; however respiratory tract pathogenic bacteria were also identified. Pasteurella haemolytica were isolated in all air samples. Nine species were found in the a half of the soil samples. The most frequent species in leachate were Acinetobacter baumanii, Bordetella sp, Brucella sp. and Escherichia coli var II. The occurrence of pathogenic and opportunistic species points out to the nosocomial and domestic clinical wastes discharged in the landfill as a potential risk for public and occupational health.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27589385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confirmation of presumptive Salmonella colonies contaminated with Proteus swarming using the polymerase chain reaction (PCR) method. 用聚合酶链反应(PCR)法确定被变形杆菌群污染的沙门氏菌菌落。

Revista latinoamericana de microbiologia Pub Date : 2007-01-01

Rosalba Gutiérrez Rojo, Edith Torres Chavolla

{"title":"Confirmation of presumptive Salmonella colonies contaminated with Proteus swarming using the polymerase chain reaction (PCR) method.","authors":"Rosalba Gutiérrez Rojo, Edith Torres Chavolla","doi":"","DOIUrl":"","url":null,"abstract":"In Mexico, zero tolerance regulation is practiced regarding Salmonella in food products. the presence of which is verified by the procedure described in NOM 114-SSA-1994. During the period between August 2002 and March 2003, 245 food samples were tested using this procedure in the Central Laboratories of the Department of Health for the State of Jalisco (CEESLAB). Of these 245 samples, 35 showed presumptive colonies contaminated with Proteus swarm cells even after selective isolation. These swarm cells make Salmonella recovery and biochemical identification difficult due to the occurance of atypical biochemical profiles which generally correspond to that of Proteus. Out of the 35 samples contaminated with Proteus, 65 presumptive colonies were isolated. These colonies were analyzed using both normative microbiological method and Polymerase Chain Reaction (PCR). The PCR method detected two positive samples while normative microbiological method was not able to identify. In order to determine the extent of interference of Proteus swarming on the Salmonella-specific PCR band amplification, Salmonella ser. Typhimurium was grown in the presence of Proteus swarming. These results show that Proteus swarming did not interfere with Salmonella PCR-amplification, although the appearance of Sanlmonella was altered such that the black precipitate was no observed in the presence of Proteus swarming. Ours result indicate that the PCR method used in this study may be successfully applied to confirm presumptive Salmnonella colonies contaminated with Proteus swarming.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27589473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary studies on the microbiological characterization of lactic acid bacteria in suero costeño, a Colombian traditional fermented milk product. 哥伦比亚传统发酵乳制品suero costeño中乳酸菌微生物学特性的初步研究。

Revista latinoamericana de microbiologia Pub Date : 2007-01-01

C Cueto, D García, F Garcés, J Cruz

{"title":"Preliminary studies on the microbiological characterization of lactic acid bacteria in suero costeño, a Colombian traditional fermented milk product.","authors":"C Cueto, D García, F Garcés, J Cruz","doi":"","DOIUrl":"","url":null,"abstract":"Suero costeño is a fermented milk product from the Colombian Atlantic coast, which is produced by the spontaneous acidification of raw milk due to the action of environmental microbes during traditional and semi-industrial processes. Eleven fermentations were carried out in experimental settings replicating traditional conditions and changes in concentration among microbial groups involved during the process (Aerobic Mesophilic bacteria, Yeasts, Enterobacteriaceae and Lactic Acid Bacteria (LAB)). LAB plays an important role in the fermentation process, especially during the final stage (24 hours). In addition, yeasts seem to have an effect on fermentation, showing an increase during the first hours of the process, while Enterobacterial counts decreased during fermentation. Thirty six LAB strains were isolated from commercial samples and thirty two were identified using the API 50 CH kit (BioMCrieux). 41% of the strains identified belonged to the species Lb. plantarum, and 19% were Lb. paracasei subsp. paracasei. Sugars fermented by LAB include milk carbohydrates such as D-Lactose, D-Glucose and D-Galactose. Because of their capacity to use other carbohydrates (manose, celobiose, maltose, fructose, ribose, trehalose, salicin, gentiobiose), it would also be possible to use these strains as starter cultures for other fermentations.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27589471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gender as a factor of susceptibility to infection in experimental hydatidosis. 性别对实验性包虫病感染易感性的影响。

Revista latinoamericana de microbiologia Pub Date : 2007-01-01

Marisol Blancas Mosqueda, Rafael Herrera Esparza, Cristina Rodríguez Padilla, Jesús Patricio Tavizón García, Marisa Mercado Reyes, Valentin Badillo Almaraz, Francisco Echavarría, Adrián López Saucedo, Carmen Mondragón de la Peña

{"title":"Gender as a factor of susceptibility to infection in experimental hydatidosis.","authors":"Marisol Blancas Mosqueda, Rafael Herrera Esparza, Cristina Rodríguez Padilla, Jesús Patricio Tavizón García, Marisa Mercado Reyes, Valentin Badillo Almaraz, Francisco Echavarría, Adrián López Saucedo, Carmen Mondragón de la Peña","doi":"","DOIUrl":"","url":null,"abstract":"We developed an experimental model of hydatidosis in BALB/c mice with six groups, a group of females and another group of males was infected with PSC of Echinococcus, granulosus. Another two groups were gonadectomised and infected with PSC. and another two groups were healthy controls. They were all bled and sacrificed after sixteen weeks post-infection. The cysts in the abdominal cavity were count, and samples were taken from liver. A microscopic study was made of the tissue around the cyst to evaluate the chronic inflammatory response. In addition the seric levels of estradiol and testosterone by means of radioimmunoassay (RIA) were determined. The results were that the females presented a greater number of cysts in liver than the males, in addition the levels to estradiol almost rose to the double in males and females after 16 weeks post-infection, and the testosterone diminished. The granulomatous response around the cysts was greater in the females than in the males. The gonadectomization affected the susceptibility to the infection in females, diminishing in number of hepatic cysts found. One concluded that the females are more susceptible to the infection by metacestode of E. granulosrus, than the males. The female displayed one better granulomatous answer than the males. Nevertheless this was not sufficient to eliminate the parasite or to inhibit its growth. The levels of estradiol and testosterone undergo an imbalance, observing that estradiol increased in chronic stages of the infection whereas the testosterone diminishes, which would indicate to us that probably the parasite causes hormonal imbalance in chronic stages, to be able to remain by long periods in its host.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27589387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCR amplification of triosephosphate isomerase gene of Giardia lamblia in formalin-fixed feces. 福尔马林固定粪便中兰氏贾第虫三磷酸异构酶基因的PCR扩增。

Revista latinoamericana de microbiologia Pub Date : 2007-01-01

Nora Molina, Daniela Polverino, Marta Minvielle, Juan Basualdo

引用次数: 0

[Hydrodynamic stress: death and cellular damage in agitated cultures]. [流体动力应力:搅拌培养中的死亡和细胞损伤]。