Revista latinoamericana de microbiologia最新文献_第5页

Incidence of salmonellosis and identification of serogroups and serotypes in a pig commercial farm in Yucatan. 尤卡坦州一个养猪场沙门氏菌病发病率及血清群和血清型鉴定。

Revista latinoamericana de microbiologia Pub Date : 2006-01-01

J C Rodríguez-Buenfil, M Alvarez-Fleites, J C Segura-Correa

{"title":"Incidence of salmonellosis and identification of serogroups and serotypes in a pig commercial farm in Yucatan.","authors":"J C Rodríguez-Buenfil, M Alvarez-Fleites, J C Segura-Correa","doi":"","DOIUrl":"","url":null,"abstract":"A study was conducted in order to detect the presence of Salmonella spp in fattening pigs, to identify the serogroups present and to determine the sensibility to the antibiotics more used in the region. The farm was a breeding farm of a multiple-site system. Of the total farrowings of a week, 55 sows and one piglet from each sow were selected. All pigs were negative to Salmonella spp. at the star of the study. Piglets were monitored from day two of age (six times; every 23 days approximately) up to finishing (23 weeks of age). Samples of feces (1 g/animal) were collected directly from the pig's rectum. The first positive pig was found at the second sampling (25 days) and the highest number of positive cases in the fifth sampling (117 days). The cumulative incidence was 52.7%. Thirty-four out of the 40 Salmonellas isolated corresponded to the B serogroup and 6 to the C2 serogroup. The serotypes found in the B serogroup were: S. typhimurium (28/34) and S. agona (6/34). Regarding serogroup C2 these were: S. romanby and S ajiobo. Salmonella spp B serogroup included three of the serotypes more commonly isolated in humans: S. typhimurium, S. agona and S. heidelberg.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"48 1","pages":"10-3"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26602466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Stationary phase in Escherichia coli]. [大肠杆菌固定相]。

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Jesús Ramírez Santos, Gabriel Contreras Ferrat, M Carmen Gómez Eichelmann

{"title":"[Stationary phase in Escherichia coli].","authors":"Jesús Ramírez Santos, Gabriel Contreras Ferrat, M Carmen Gómez Eichelmann","doi":"","DOIUrl":"","url":null,"abstract":"When nutrients become scarce E. coli cells enter into a non-growth phase known as stationary and develop a multiple-stress resistance state analogue to sporulation in B. subtilis. Morphological changes are observed, including rounded shape, loss of flagella and thickening of the cell wall. General metabolism is redirected, macromolecular degradation is increased, and storage and osmoprotection compounds are synthesized. The reorganization of the nucleoid is accompanied by an overall repression of gene expression, but a subset of genes required for starvation survival become transcribed in a manner dependent on the stationary phase-specific subunit of RNA polymerase (RpoS or sigma(s)). The regulatory function of sigma(s) seems to be central to a global gene network that is beginning to be understood. Also, stationary phase populations are highly heterogeneous in properties as viability, genotype, and mutability. The emergence of mutant subpopullations capable of using nutrient traces suggest survival strategies during long term starvation. This review focuses on the major characteristics of E. coli during stationary phase and on the regulatory gene network responsible of such characteristics.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"47 3-4","pages":"92-101"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro evaluation of the binding capacity of Saccharomyces cerevisiae Sc47 to adhere to the wall of Salmonella spp. 体外评价酿酒酵母Sc47对沙门氏菌壁的粘附能力。

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Luis Salvador Pérez-Sotelo, Martín Talavera-Rojas, Humberto G Monroy-Salazar, Salvador Lagunas-Bernabé, José A Cuarón-Ibargüengoytia, Roberto Montes de Oca Jimenez, Juan Carlos Vázquez-Chagoyán

引用次数: 0

[Lipopolysaccharides of rhizobiaceae: structure and biosynthesis]. 根瘤菌科脂多糖:结构与生物合成

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Ernesto Ormeño-Orrillo

引用次数: 0

[Proteins with GGDEF and EAL domains: their role in bacterial metabolism]. [具有GGDEF和EAL结构域的蛋白质:它们在细菌代谢中的作用]。

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Micaela Marcela Méndez-Ortiz, Jorge Membrillo-Hernández

引用次数: 0

[Molecular approaches to the control of onchocerciasis in Mexico]. [墨西哥控制盘尾丝虫病的分子方法]。

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Mario A Rodríguez-Pérez

引用次数: 0

Comparison of two methods of PCR followed by enzymatic restriction digestion for detection and typing of herpes simplex viruses isolated from patients with mucocutaneous or cutaneous lesions. 两种PCR -酶切酶切法检测皮肤粘膜或皮肤病变患者单纯疱疹病毒并分型的比较

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Emma Herrera-Martínez, Rodolfo Ondarza-Aguilera, Sergio Estrada-Parra, Guillermo Pérez, Blanca L Barrón

{"title":"Comparison of two methods of PCR followed by enzymatic restriction digestion for detection and typing of herpes simplex viruses isolated from patients with mucocutaneous or cutaneous lesions.","authors":"Emma Herrera-Martínez, Rodolfo Ondarza-Aguilera, Sergio Estrada-Parra, Guillermo Pérez, Blanca L Barrón","doi":"","DOIUrl":"","url":null,"abstract":"A multitude of different polymerase chain reactions (PCRs) have been described for detection and typing of Herpes simplex virus (HSV). This paper compares two PCRs coupled to enzymatic restriction (PCR/RFLP) to detect and type HSV. A primers set was designed to amplify a HSV DNA fragment from UL30 and UL 15 genes. Typing was done by restriction of the UL30 and UL15 amplicons with Ava II and Hpa II enzymes, respectively. This strategy was tested with two reference strains (HSV-1 McIntyre, and HSV-2 G), and 47 clinical HSV isolates. Both PCRs produced the expected amplicons (a 492 bp UL30, and 305 bp UL15). The restriction of both amplicons clearly differentiated HSV- from HSV-2, and produced equal results. Thirty one (66%) of the isolates were identified as HSV-1, and the other 16 (34%), as HSV-2. Most of the HSV-1 isolates (27/31) were from orofacial and thoracic lesions; and also, one half of the HSV-2 isolates (8/16) were from the same anatomical regions. Our results showed that either of the two PCR/RFLP could be used to detect and type HSV. Furthermore, our results of the anatomical site of HSV-1 and HSV-2 infections are consistent with previous reports which have shown changes in the classical anatomical localization of herpesvirus infections.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"47 3-4","pages":"76-81"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enteroaggregative Escherichia coli an emergent pathogen with different virulence properties. 肠聚集性大肠杆菌是一种具有不同毒力特性的突发性病原体。

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

J M Villaseca, U Hernández, T R Sainz-Espuñes, C Rosario, C Eslava

{"title":"Enteroaggregative Escherichia coli an emergent pathogen with different virulence properties.","authors":"J M Villaseca, U Hernández, T R Sainz-Espuñes, C Rosario, C Eslava","doi":"","DOIUrl":"","url":null,"abstract":"Enteroaggregative Escherichia coli (EAEC) is an emergent bacterial pathogen. The first studies in developing countries with EAEC strains, showed that this bacterium was associated with persistent diarrhea. However, new studies showed that EAEC may be associated also with acute diarrhea, with both nosocomial and community outbreaks worldwide, and as an important pathogen of diarrheal disease in human immunodeficiency virus-infected adults. EAEC strains are recognized by their characteristic aggregative adherence or \"stacked-brick\" pattern to epithelial cells. Although the pathogenesis of EAEC infection is not well understood, cellular changes observed in animal models and in vitro assays, suggested that the alterations in the intestinal mucosa during EAEC infection are associated with adherence factors and toxins production. The damage has been associated with the release of inflammatory mediators, which may contribute also to the intestinal illness. The dissemination of the high pathogenicity island from Yersinia pestis evolutionary group to EAEC has been show; different studies suggest that it may contribute to the virulence of EAEC strains. Molecular methods to investigate the presence of plasmid and chromosomal EAEC-associated virulence markers, have been used for the characterization and epidemiological studies of EAEC strains. Although the clinical and epidemiological importance of EAEC have been demonstrated in different studies, Escherichia coli strains with adherent agreggative phenotype are commonly isolated from healthy children and environmental sources. This support the necessity to study virulence factors no related with the cells adherence pattern, that show the specific EAEC pathogenic clones associated whit intestinal disease.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"47 3-4","pages":"140-59"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survival of Brucella abortus in milk fermented with a yoghurt starter culture. 流产布鲁氏菌在用酸奶发酵剂发酵的牛奶中的存活。

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Armida Zúñiga Estrada, Lydia Mota de la Garza, Miroslava Sánchez Mendoza, Eva María Santos López, Santiago Filardo Kerstupp, Ahidé López Merino

{"title":"Survival of Brucella abortus in milk fermented with a yoghurt starter culture.","authors":"Armida Zúñiga Estrada, Lydia Mota de la Garza, Miroslava Sánchez Mendoza, Eva María Santos López, Santiago Filardo Kerstupp, Ahidé López Merino","doi":"","DOIUrl":"","url":null,"abstract":"In countries such as Mexico, brucellosis is still an important public health problem due to the consumption of non-pasteurized milk and dairy products, contaminated with Brucella spp. The aim of this study was to look into the survival of Brucella abortus during fermentation of milk with a yoghurt starter culture and storage at refrigeration temperature. Sterile skim milk was inoculated with B. abortus at two concentrations, 10(5) and 10(8) CFU/ml simultaneously with a yoghurt starter culture of lactic acid bacteria (Streptococcus thermophilus and Lactobacillus delbrueckii subspecie bulgaricus). Inoculated flasks were incubated at 42 degrees C, followed by refrigeration at 4 degrees C. Samples were taken during fermentation and during storage and viable count of B. abortus and lactic acid bacteria and pH were determined. Results showed that after 10 days of storage at 4 degrees C, B. abortus was recovered in fermented milk at a level of 10(5) CFU/ml, despite the low pH below 4.0. Therefore B. abortus is able to survive in fermented milk. This finding may imply that non-pasteurized fermented milk contaminated with Brucella abortus could be a means of transmission of these bacteria.","PeriodicalId":21464,"journal":{"name":"Revista latinoamericana de microbiologia","volume":"47 3-4","pages":"88-91"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26327795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Antimicrobial mechanisms and potential clinical application of lactoferrin]. 乳铁蛋白的抗菌机制及潜在临床应用

Revista latinoamericana de microbiologia Pub Date : 2005-07-01

Dora Alicia Rodríguez-Franco, Luz Vázquez-Moreno, Gabriela Ramos-Clamont Montfort

引用次数: 0