Scanning electron microscopy最新文献_第6页

Topical modes in the preparation of human spleen specimens for routine scanning electron microscopy studies. 用于常规扫描电镜研究的人脾标本制备的局部模式。

Scanning electron microscopy Pub Date : 1986-01-01

H Gamliel, H M Golomb, E Avinoah, D Gurfel, A Polliack

引用次数: 0

Beam voltage effects in the study of embedded biological materials by secondary electron detectors. 二次电子探测器在嵌入式生物材料研究中的束流电压效应。

Scanning electron microscopy Pub Date : 1986-01-01

C Scala, G Pasquinelli, P Preda, R Laschi

引用次数: 0

Review of statoconia formation in birds and original research in chicks (Gallus domesticus). 鸟类静蛋形成及雏鸟(Gallus domesticus)的初步研究综述。

Scanning electron microscopy Pub Date : 1986-01-01

C D Fermin, M Igarashi

{"title":"Review of statoconia formation in birds and original research in chicks (Gallus domesticus).","authors":"C D Fermin, M Igarashi","doi":"","DOIUrl":"","url":null,"abstract":"This paper reviews published materials on statoconia formation in birds, and emphasizes works dealing with the embryonic chick (Gallus domesticus) saccule and utricule. Histological, biochemical and histochemical aspects of forming statoconial membranes and statoconial crystals of mammals are included. Results from our work with chick embryos permitted us to conclude that statoconia probably do not form by seeding of a subunit around central core. Instead, immature statoconia may emerge already formed, from a segmenting mass of organic material that seems to be secreted by the supporting cells of the saccular and utricular maculae. Crystallization of each statoconium may involve seeding of multiple subunits around many nucleation centers in the organic matrix. Following these processes, calcium (sometimes granular) attaches to immature statoconia and become subsequently incorporated between the fibrils of the organic matrix starting at the peripheral zone and advancing toward the central core. Our transmission electron microscopy findings, histochemistry and X-ray microanalysis complements of other investigators, who used chicks with light microscopic studies. These results agree with the notion that the secretion of an organic matrix constitutes the first step toward the formation of the statoconial membrane and statoconia. We show ultrastructurally how statoconia may be assembled from the organic matrix before they acquire their characteristic geometric shapes.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 4","pages":"1649-65"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14667311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative renal vascular casting in nephrology research. 定量肾血管铸型在肾脏病学研究中的应用。

Scanning electron microscopy Pub Date : 1986-01-01

V H Gattone, A P Evan

{"title":"Quantitative renal vascular casting in nephrology research.","authors":"V H Gattone, A P Evan","doi":"","DOIUrl":"","url":null,"abstract":"The present paper describes the use of a quantitative renal vascular casting method to study the changes associated with kidney disease. Several animal models of hypertension (spontaneously hypertensive rat, SHR, with its normotensive rat the Wistar Kyoto, WKY; Dahl salt sensitive DS - hypertensive, and salt resistant DR - normotensive) were examined at time points when the systemic blood pressure was rising (6 and 12 weeks of age) and following renal denervation (in SHR-WKY rats). The SHR appears to have a smaller caliber afferent arteriole at both 6 and 12 weeks of age. This difference is probably not entirely due to sympathetic vasoconstriction since the strain related afferent arteriolar diameter difference was still present after renal denervation. In the Dahl rats, there is not much of an intrarenal vascular difference between the DS and DR rats with the only real finding of a smaller distal afferent arteriolar diameter found in outer cortical nephrons of the DR. The two models of acute renal failure (ARF) that were studied include, the glycerol model (known to initially cause an intense vasoconstriction) and gentamicin, a nephrotoxic antibiotic. Two time points were examined for each of these models. As expected in the glycerol model there was an intense vasoconstriction at three hours which essentially was gone at 3 days - a time when the renal failure was fulminant. The glomerulus appeared to be contracted at three hours as well. In the gentamicin model no renal vascular alteration was seen at 6 days, when renal failure was mild while at 10 days, when renal failure was pronounced, outer cortical afferent arterioles appeared to be moderately constricted. In the 5/6 nephrectomy model of chronic renal failure, the glomeruli were smaller in rats in renal failure than in the controls.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 1","pages":"253-62"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14856392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scanning electron microscopy of human metaphase chromosomes. 人类中期染色体的扫描电镜。

Scanning electron microscopy Pub Date : 1986-01-01

T D Allen, E M Jack, C J Harrison, D Claugher

{"title":"Scanning electron microscopy of human metaphase chromosomes.","authors":"T D Allen, E M Jack, C J Harrison, D Claugher","doi":"","DOIUrl":"","url":null,"abstract":"Preparative methods for scanning electron microscopy of chromosomes are dependent on the original source of material. Chromosomes extracted from unfixed metaphase cells via isolation buffers tend to show topography and surface morphology which may have been induced by the choice of isolation buffer itself. Furthermore, this type of preparation often precludes any chromosome identification, as many metaphases have been pooled, and also the chromosomes from these preparations are not suitable for the banding techniques regularly used in clinical cytogenetics. Our own approach has been to use the standard cytogenetic approach, starting with methanol-acetic acid fixed, air dried metaphase spreads, allowing both identification of individual chromosomes, and also the facility for various banding procedures such as G and C banding to be performed. Chromosomes are subsequently \"reprepared\" for SEM, using rehydration, glutaraldehyde fixation, and osmium impregnation using Thiocarbohydrazide (TCH). This method produces chromosomes which can be examined at high resolution, without metallic coating, for their topography, surface morphology and chromatin organisation, and the changes produced by banding techniques which give rise to a structural alterations resulting in differential staining in the light microscope.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 1","pages":"301-8"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14856394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface morphology of unused and used Hydromer-coated intravenous catheters. 未使用和已使用的涂膜静脉导管的表面形貌。

Scanning electron microscopy Pub Date : 1986-01-01

A Bylock, E Hultman, B Gustavsson, L E Linder, I Curelaru

{"title":"Surface morphology of unused and used Hydromer-coated intravenous catheters.","authors":"A Bylock, E Hultman, B Gustavsson, L E Linder, I Curelaru","doi":"","DOIUrl":"","url":null,"abstract":"Hydromer-coated polyurethane (Erythroflex) catheters, unused, or intravenously inserted for 2-20 days, were studied by scanning electron microscopy (SEM). Both unfixed and fixed (2% glutaraldehyde in phosphate buffer), and air-or critical-point dried (CPD) specimens were investigated. The catheter segments were sputter-coated with approx. 20 nm gold and studied at an accelerating voltage of 20 kV. The specimens were examined for surface depositions, thickness and structure of the Hydromer layers, and occurrence of adhering and embedded bacteria. The outer Hydromer layer showed, in the unused specimens, scratches and fissures, as well as adhering foreign bodies. In used specimens, the layer was swollen, with cracks (like \"dried earth\"), and, occasionally, amorphous substances and coccoid bacteria were seen adhering. Damage to the layer, or even its total disappearance was also noted in some specimens. The inner (luminal) Hydromer layer was, in unused specimens, clean and slightly wavy. In used catheters, it was thicker, possibly swollen, with small, isolated or agglomerated protrusions, like a \"lunar landscape\". Adhering platelets and amorphous substances were also occasionally seen. The results suggest that the Hydromer is a fragile material in both its dry and wet forms. Thus, the Hydromer-coated catheters should neither be stored in flexible packs, nor inserted by the Seldinger technique. The findings do not support the belief that the Hydromer-coating can prevent either thrombus formation, or intraluminal occlusion of the in-situ catheters.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 1","pages":"157-64"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14857575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Otoconia as test masses in biological accelerometers: what can we learn about their formation from evolutionary studies and from work in microgravity? 耳蜗作为生物加速度计的测试质量:我们能从进化研究和微重力工作中了解到它们的形成吗?

Scanning electron microscopy Pub Date : 1986-01-01

M D Ross, K M Donovan

引用次数: 0

Lack of regional surface differences in mouse bladder urothelium: a scanning electron microscopic study. 小鼠膀胱尿路上皮缺乏区域表面差异:扫描电镜研究。

Scanning electron microscopy Pub Date : 1986-01-01

K Feren, J B Reitan

引用次数: 0

Scanning electron microscopic study of the surface of feline gastric epithelium: a simple method of removing the coating material. 猫胃上皮表面的扫描电镜研究:一种去除涂层物质的简单方法。

Scanning electron microscopy Pub Date : 1986-01-01

M Al-Tikriti, R W Henry, F K Al-Bagdadi, J Hoskins, C Titkemeyer

引用次数: 0

Preparation methods for quantitative electron probe X-ray microanalysis of rat exocrine pancreas: a review. 大鼠外分泌胰腺定量电子探针x射线制备方法综述。

Scanning electron microscopy Pub Date : 1986-01-01

N Roos, T Barnard

{"title":"Preparation methods for quantitative electron probe X-ray microanalysis of rat exocrine pancreas: a review.","authors":"N Roos, T Barnard","doi":"","DOIUrl":"","url":null,"abstract":"Pancreatic acinar cells are thought to secrete a fluid containing digestive enzymes and electrolytes and use e.g. calcium as a second messenger upon stimulation. Together with their pronounced morphological polarity, they provide a model system to study the effect of different preparation methods for quantitative biological electron probe X-ray microanalysis (EPXMA) of ultrathin sections. Several preparation methods i.e., freeze-drying and plastic-embedding, freeze-substitution (2 days) and freeze-drying of ultrathin cryosections have been applied to examine the retention of sodium, magnesium, phosphorus, sulfur, (chlorine), potassium and calcium in subcellular compartments (basal cytoplasm, apical cytoplasm, mitochondria and zymogen granules). In freeze-substituted samples the phosphorus, potassium and sulfur concentrations were 2-3 times lower in all compartments compared to freeze-dried, plastic-embedded samples. Intracellular potassium-to-sodium ratios obtained on frozen substituted and frozen-dried, plastic-embedded samples were considerably lower than for cryosections. Element gradients between adjacent organelles were large in frozen-dried cryosections, smaller in frozen-dried plastic- embedded samples and insignificant in frozen-substituted samples.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 2","pages":"703-11"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14663758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0