Scanning electron microscopy最新文献_第7页

The determination of wet weight concentrations of elements in freeze-dried cryosections from biological cells. 生物细胞冻干切片中元素湿重浓度的测定。

Scanning electron microscopy Pub Date : 1986-01-01

K Zierold

引用次数: 0

Technical parameters of plastics (Mercox CL-2B and various methylmethacrylates) used in scanning electron microscopy of vascular corrosion casts. 用于血管腐蚀铸件扫描电镜的塑料(Mercox CL-2B和各种甲基丙烯酸甲酯)技术参数。

Scanning electron microscopy Pub Date : 1986-01-01

T Weiger, A Lametschwandtner, P Stockmayer

引用次数: 0

Surface studies of duodenal lesions induced by thoracic irradiation. 胸廓照射致十二指肠病变的表面研究。

Scanning electron microscopy Pub Date : 1986-01-01

K E Carr, S Ellis, A Michalowski

{"title":"Surface studies of duodenal lesions induced by thoracic irradiation.","authors":"K E Carr, S Ellis, A Michalowski","doi":"","DOIUrl":"","url":null,"abstract":"Acute duodenal ulcers are produced in mice as a remote (\"abscopal\") effect of irradiation to the lower mediastinum. Such lesions have been examined with scanning electron microscopy at 5, 8 and 28 days after irradiation with 18 Gy of X-rays. All the ulcers occur within the first 1 cm long segment of the duodenum which is endowed with Brunner's glands. The single lesions vary in size, shape and position. The damaged area often includes much of the duodenal circumference and is distinguished by conical or rudimentary villi, or even by the complete absence of villi. In contrast, around the periphery of the ulcer the villi are mostly vertical. Although the floor of these lesions appears to be covered with a continuous epithelial layer, during the first 4 weeks after irradiation the severity of the focal duodenal damage seems to increase gradually with time. The lesions have been compared with specimens from unirradiated mice and also with samples taken 3 days after partial thoracic irradiation when little damage is seen. The pattern of fully developed duodenal lesions differs greatly from that seen after direct irradiation where damage has not included localised ulceration in the samples of jejunum so far examined. The lesions induced by partial thoracic irradiation may be related to radiation injury to vascular or autonomic nerve targets in the lower mediastinum. Such injury could result in malfunction of the pyloric sphincter or could alter the secretion by Brunner's glands and thus lead to duodenal ulceration.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 1","pages":"209-19"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14856507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of hematopoiesis in long term marrow cultures: role of humoral factors in the proliferation and differentiation of stem cells and committed progenitors. 长期骨髓培养中造血功能的调节:体液因子在干细胞和承诺祖细胞增殖和分化中的作用。

Scanning electron microscopy Pub Date : 1986-01-01

C E Eastment

引用次数: 0

Structural features of isolated, fractionated bone marrow endothelium compared to sinus endothelium in situ. 分离骨髓内皮与原位窦内皮的结构特征比较。

Scanning electron microscopy Pub Date : 1986-01-01

S Irie, M Tavassoli

{"title":"Structural features of isolated, fractionated bone marrow endothelium compared to sinus endothelium in situ.","authors":"S Irie, M Tavassoli","doi":"","DOIUrl":"","url":null,"abstract":"Structural features of isolated, fractionated rat bone marrow endothelium were compared to those of marrow sinus endothelium in situ. Marrow endothelium was purified, first by density gradient sedimentation on Percoll and then subjected to centrifugal elutriation. Using antifactor VIII antibody staining (indirect immunofluorescent method), preparations of greater than 50% purified endothelium were obtained. By SEM, these cells were about 10 microns in size and showed smooth surface and numerous invaginations. These features were also observed in the in situ endothelium obtained by perfusion-fixation and freeze-cracking. In addition, in situ endothelium displayed numerous hemopoietic cells in migration through the endothelium. By TEM, isolated endothelium showed numerous vesiculations, giving the cell sponge-like appearance. This corresponded to numerous intracellular vesicles in sinus endothelium in situ, reflecting high magnitude of fluid and molecular transport across the endothelium. Weibel-Palade bodies were not seen in either form of the endothelium, despite the positive reaction for factor VIII-related antigen. This finding suggested that the cell, while possessing factor VIII-related antigen, does not store this protein.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 2","pages":"615-9"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14917026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation in the sizes of resorption lacunae made in vitro. 体外吸收腔隙大小的变化。

Scanning electron microscopy Pub Date : 1986-01-01

S J Jones, A Boyde, N N Ali, E Maconnachie

{"title":"Variation in the sizes of resorption lacunae made in vitro.","authors":"S J Jones, A Boyde, N N Ali, E Maconnachie","doi":"","DOIUrl":"","url":null,"abstract":"The assessment of in vitro osteoclastic activity has, until recently, been dependent on the analysis of organ culture experiments. We have developed a single cell resorption assay so that the resorptive function of individual osteoclasts could be studied. This paper examines the biological variation in the sizes of resorption lacunae produced by bone cell cultures derived from neonate rats and rabbits, and prehatch or hatchling chicks. Cultures were run for 24h for all species; and in addition for 48h for rat, 9 or 12 hours for rabbit and 3-7 hours for chick. The numbers of the nuclei of osteoclasts seeded on to plastic were counted for all three species. SEM stereophotogrammetry was used to measure areas, volumes, and maximum and average depths of the lacunae using specially designed instruments and software. Rat osteoclasts were smallest, and more chick osteoclasts were very large. There was a species difference in the onset of resorption and the sizes of pits produced, the chick osteoclasts being more vigorous resorbers than the rabbit ones, and the rat least so. For a given plan area, chick lacunae were deeper. There was a high correlation between area and volume. The range of maximum depths for a given area was high, however. Thus the mean of a few measurements of depths should not be used to calculate volume from area. At 24 hours, 77% of the rat, 47% of the rabbit and 28% of the chick lacunae were less than 1,000 microns 3 in volume; and 11% of the rat, 17% of the rabbit and 22% of the chick lacunae were between 1,000 and 2,000 microns 3 in volume. The mean values at 24 hours were 981, 2796, and 4582 microns 3 for rat, rabbit and chick lacunae respectively.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 4","pages":"1571-80"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14926043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential scanning electron microscopic analyses of normal and spontaneously occurring abnormal ocular development in C57B1/6J mice. C57B1/6J小鼠正常及自发眼发育异常的序列扫描电镜分析。

Scanning electron microscopy Pub Date : 1986-01-01

C S Cook, K K Sulik

引用次数: 0

Opportunities for electron microscopy in space radiation biology. 电子显微镜在空间辐射生物学中的应用机会。

Scanning electron microscopy Pub Date : 1986-01-01

J T Lett

引用次数: 0

Theory for calcium-phosphate crystal formation in tissue from scanning electron microscope data. 从扫描电镜数据看组织中磷酸钙晶体形成的理论。

Scanning electron microscopy Pub Date : 1986-01-01

A C Nelson

{"title":"Theory for calcium-phosphate crystal formation in tissue from scanning electron microscope data.","authors":"A C Nelson","doi":"","DOIUrl":"","url":null,"abstract":"Scanning electron microscope (SEM) morphological analysis combined with energy dispersive characteristic x-ray analysis provides insight into the mechanism of biological mineralization. A time series of tissue micrographs and mineralization measurements can permit the determination of the mineralization kinetic behavior and is the basis upon which a computer model has been devised. The computer model is constructed from fundamental principles of crystal nucleation and precipitation theory. Various general forms of the model are tested against the laboratory data for goodness-of-fit using the least squares method, and two models are found to be acceptable. Both of the acceptable models involve inhibition of the mineralization process which has a reaction order ranging from one to two. A third model involving constant nucleation rate must be rejected. Having established working first principle models for the mineralization process, one can compute a constant number of nucleation sites and a supersaturation value for calcium in various mineralized tissues such as the spongiosa and fibrosa of heart valve leaflet implants. These quantities are determined and used in discussing a general theory for biomineralization which emphasizes therapeutic considerations.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 1","pages":"151-6"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14857573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unique scanning electron microscopic features of hairy cells in hairy-cell leukemia. A review and current status. 毛细胞白血病中毛细胞的独特扫描电镜特征。回顾和当前状态。

Scanning electron microscopy Pub Date : 1986-01-01

H Gamliel, H M Golomb

引用次数: 0