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FE-SEM Characterization of Some Nanomaterial 某些纳米材料的FE-SEM表征

Scanning electron microscopy Pub Date : 2012-03-09 DOI: 10.5772/34361

A. Alyamani, O. M. Lemine

引用次数: 27

Rare Pathological Findings 罕见病理表现

Scanning electron microscopy Pub Date : 2009-01-01 DOI: 10.1400/164226

G. Pierini, Marco Grillini, T. Balbi

引用次数: 0

The use of a line scan ratemeter for the X-ray microanalytic evaluation of membrane-bound histochemical endproducts. 使用线扫描速率计对膜结合的组织化学终产物进行x射线微分析评价。

Scanning electron microscopy Pub Date : 1986-01-01

I Nagy, V Nagy

{"title":"The use of a line scan ratemeter for the X-ray microanalytic evaluation of membrane-bound histochemical endproducts.","authors":"I Nagy, V Nagy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Although X-ray microanalysis represents a useful tool for identifying electron dense histochemical end-products, quantitative microanalytic measurements are seriously hampered in the case of the activities of certain membrane-bound enzymes. For example, the electron histochemical methods revealing K+-dependent pNPPase activity result in a very fine, granular reaction product of lead phosphate. Therefore microanalytic, densitometric of similar evaluations of the reaction, even in semiquantitative terms are not practical by the usual procedures. This paper describes a method of X-ray microanalysis of thick sections (0.5 micron) processed for K+-pNPPase, where a sufficient amount of lead is present for X-ray microanalytic determination. The analysis is performed in the line scan mode on transversely cut membrane profiles by means of the line scan ratemeter of an EDAX System F. This yields quantitative data on the relative lead concentrations in the vicinity of the cell membrane. A method is proposed for calculation of relative enzyme activities based on the Pb-signal of the ratemeter curve and the average \"noise\"-level of the cytoplasm, containing also non-specifically bound lead. This method avoids the necessity of measuring the section thickness; it may be useful for a variety of purposes in the electron microscopic histochemistry of membrane-bound enzymes.</p>","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 2","pages":"725-34"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14158713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The morphologic categorization of cell death induced by mild hyperthermia and comparison with death induced by ionizing radiation and cytotoxic drugs. 亚热疗致细胞死亡的形态学分类及与电离辐射和细胞毒药物致细胞死亡的比较。

Scanning electron microscopy Pub Date : 1986-01-01

D J Allan, B V Harmon

{"title":"The morphologic categorization of cell death induced by mild hyperthermia and comparison with death induced by ionizing radiation and cytotoxic drugs.","authors":"D J Allan, B V Harmon","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper presents a summary of the morphological categorization of cell death, results of two in vivo studies on the cell death induced by mild hyperthermia in rat small intestine and mouse mastocytoma, and a comparison of the cell death induced by hyperthermia, radiation and cytotoxic drugs. Two distinct forms of cell death, apoptosis and necrosis, can be recognized on morphologic grounds. Apoptosis appears to be a process of active cellular self-destruction to which a biologically meaningful role can usually be attributed, whereas necrosis is a passive degenerative phenomenon that results from irreversible cellular injury. Light and transmission electron microscopic studies showed that lower body hyperthermia (43 degrees C for 30 min) induced only apoptosis of intestinal epithelial cells, and of lymphocytes, plasma cells, and eosinophils. In the mastocytoma, hyperthermia (43 degrees C for 15 min) produced widespread tumor necrosis and also enhanced apoptosis of tumor cells. Ionizing radiation and cytotoxic drugs are also known to induce apoptosis in a variety of tissues. It is attractive to speculate that DNA damage by each agent is the common event which triggers the same process of active cellular self-destruction that characteristically effects selective cell deletion in normal tissue homeostasis.</p>","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 3","pages":"1121-33"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14231000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryoultramicrotomy and immunocytochemistry in the analysis of muscle fine structure. 冷冻切片和免疫细胞化学在肌肉精细结构分析中的应用。

Scanning electron microscopy Pub Date : 1986-01-01

L E Thornell, G S Butler-Browne, E Carlsson, H M Eppenberger, D O Fürst, B K Grove, B Holmbom, J V Small

引用次数: 0

Functional aspects of renal glomeruli based on scanning electron microscopy of corrosion casts, with special emphasis on reptiles and birds. 基于腐蚀铸件扫描电子显微镜的肾小球功能方面，特别强调爬行动物和鸟类。

Scanning electron microscopy Pub Date : 1986-01-01

H Ditrich, H Splechtna

引用次数: 0

Ultrastructure of bone marrow of rats after severe hemodilution with starch or modified hemoglobin. 淀粉或改性血红蛋白重度血液稀释后大鼠骨髓超微结构。

Scanning electron microscopy Pub Date : 1986-01-01

F R Campbell, R D Rink

引用次数: 0

Ciliated cells of the trachea of the rabbit, treated with cis-diamminedichloroplatinum (II) alone, or in combination with ionizing radiation. 兔气管纤毛细胞，用顺式二胺二氯铂(II)单独或与电离辐射联合处理。

Scanning electron microscopy Pub Date : 1986-01-01

M Albertsson, C H Hakansson, C Mercke

{"title":"Ciliated cells of the trachea of the rabbit, treated with cis-diamminedichloroplatinum (II) alone, or in combination with ionizing radiation.","authors":"M Albertsson, C H Hakansson, C Mercke","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ciliated epithelium of the rabbit trachea was irradiated with daily fractions of 2 Gy to an accumulated dose of 20 Gy (TD: 2, 6, 10, 16, or 20 Gy). Fifteen to forty-five minutes before start of the first irradiation (treatment day 1), 5 mg cis-DDP was given by intraperitoneal injection to each rabbit. Examination was made 1 - 10 days after each fractionation schedule, when specimens were taken for investigations. Scanning electron microscope investigations showed a gradual development of ciliary damage from blebs on the cilia to swollen tips, broken and bent cilia and finally an epithelial lining with areas free from cilia with a surface covered with microvilli-like structures. SEM also showed cell loss, and remnants of dead cells on the surface together with detritus. By transmission electron microscope ciliary damage, cell death and cell loss of the ciliated cell layer as well as exfoliation of portions of goblet-like cells on the surface could be confirmed. The irradiated ciliated epithelium and the untreated control epithelium in each animal showed no difference in this respect. Thus no enhancement of the effects of radiation could be observed. The development of ultrastructural damage may be due to a cytotoxic effect of the drug on the ciliated epithelium. However, 19 days after the start of cis - DDP injection, a hyperplasia of the basal cell layer was observed, which indicates that the observed cytotoxicity of the drug is reversible and a normalisation occurs during the last days of observation in this study.</p>","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 3","pages":"1109-19"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14915832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcification in aging canine aortic valve. 衰老犬主动脉瓣的钙化。

Scanning electron microscopy Pub Date : 1986-01-01

K M Kim, S H Chang, B F Trump, H Spurgeon

引用次数: 0

Changes in absorptive surfaces of rat visceral yolk sac with increasing gestational age. 大鼠内脏卵黄囊吸收面随胎龄增加的变化。

Scanning electron microscopy Pub Date : 1986-01-01

W P Jollie

{"title":"Changes in absorptive surfaces of rat visceral yolk sac with increasing gestational age.","authors":"W P Jollie","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The free surface of the endodermal epithelial lining of the rat visceral yolk sac was examined by scanning electron microscopy at three stages of pregnancy, viz., 12, 17 and 22 days (birth typically occurring on the 23rd day). Additionally, by ultrasonic vibration of tissues that had been subjected to prolonged osmium fixation, the epithelium was removed and such microdissected membranes similarly were examined. With increasing gestational age the free surface of the epithelium underwent a relative increase in absorptive area by three mechanisms: formation of increasingly complex villous projections of the visceral yolk sac as a whole, a doming of the individual epithelial cells, and an increase in length and number of microvilli for each such cell. The resultant increase in surface area, however, could not be correlated with the onset of receptor mediated endocytosis at the latest stage. The basement membrane underlying the lining epithelium of the yolk sac easily was revealed by ultrasonication of well-fixed tissues; it was most fragile at 12 days at which time the procedure apparently either removed the lamina lucida of the basal lamina, exposing a fibrillar component of the lamina densa, or removed the entire basal laminar component of the basement membrane, exposing an underlying reticular lamina. At 17 days the basement membrane at the cores of denuded villi showed the negative impressions of the epithelial cells that had been removed; and at 22 days, when the membrane is known to be permeable to protein transfer, it was smooth, featureless, and appeared most durable.</p>","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 2","pages":"661-9"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14917029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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