Russian Open Medical Journal最新文献

{"title":"Effect of lncRNA RMRP on proliferation, apoptosis, osteoblast differentiation and mineralization of bone marrow mesenchymal stem cells","authors":"Jingyuan Guo, Yiwang Man, Shuai Xiang, Xiaomei Zhong, Genyun Tang, Jiefu Tang","doi":"10.15275/rusomj.2025.0204","DOIUrl":"https://doi.org/10.15275/rusomj.2025.0204","url":null,"abstract":"Background — Long non-coding RNAs (lncRNAs) play a crucial role in bone growth and skeletal development. Ribonuclease (RNase) for mitochondrial RNA processing, commonly known as RMRP, is a small nucleolar ribonucleoprotein particle composed of RMRP lncRNA (lncRNA RMRP) and several protein subunits. Specifically, mutations in lncRNA RMRP are associated with cartilage-hair hypoplasia and skeletal developmental disorders in humans. For instance, mutations in lncRNA RMRP are observed in individuals with severe dwarfism, thus suggesting that RMRP functions in both chondrogenic and osteogenic processes. Results of a previous study have identified a potential role of RMRP in chondrogenic differentiation in mouse cell lines. However, the specific functions of RMRP in osteogenic processes remain unclear. Methods — In the present study, RMRP expression was altered in murine bone marrow derived mesenchymal stem cells (BMSCs). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), cell counting, apoptosis and mineralization assays were used to investigate the effects of RMRP on BMSCs, osteoblasts and osteogenic differentiation. Results — The results of the present study showed that RMRP expression was up-regulated in BMSCs and down-regulated during apoptosis. The RMRP knockdown resulted in decreased proliferation and viability of BMSCs and increased apoptosis. In addition, the RMRP knockdown increased osteoblast differentiation of BMSCs and affected the mineralization of osteoblastic cells. Conclusion — The results of the present study indicated that RMRP may play a role in BMSCs, osteoblastic cells and osteogenic differentiation process. Therefore, the present study may provide a new theoretical basis for the role of RMRP in skeletal development.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"14 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Endophytes from Rice (Oryza sativa); Enrichment Strategies, Isolation and Plant Growth Promotion","authors":"Khouri Chalouhi, Denise","doi":"10.21954/ou.ro.00106943","DOIUrl":"https://doi.org/10.21954/ou.ro.00106943","url":null,"abstract":"Rice is a major agricultural crop essential to global food security. As population growth increases food demand, improving rice yield and resistance to abiotic stresses is critical, especially under climate change. Microorganisms form complex ecological networks, and bacterial members of plant-associated microbiomes significantly impact plant health. Plant associated bacteria (PAB) colonize different plant-associated niches and soil, being a major microbial reservoir, supports diverse bacteria, making roots hotspots for colonization. Bacterial root endophytes inhabit internal root tissues (endosphere), actively sensing the plant environment and plant signals and adapting behavior to confer benefits such as nitrogen fixation, stress tolerance, and growth promotion. Given consistent patterns of bacterial recruitment under specific environmental and growth conditions, we hypothesize that endophytes enhance rice growth and abiotic stress resistance. This study aims to identify and characterize super-endophytic bacteria highly active in rice endosphere colonization, their multispecies communities, and their cell-to-cell signaling mechanisms. On the latter subject, we will particularly investigate the regulation of the Quorum Sensing system in three endophytic Pantoea species isolated from the rice endosphere. Additionally, we will characterize endophytes that confer resistance to salinity and nitrogen starvation, with potential agricultural applications as biofertilizers or probiotics.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elderly Care: Commonly Prescribed Gastrointestinal Drugs Associate With Cognitive Decline","authors":"Amirmohammad Rezaei Majd, Simin Mouodi, Ali Akbar Moghadamnia, Sussan Moudi, Ali Bijani, Seyed Reza Hosseini, Reza Ghadimi","doi":"10.15275/rusomj.2024.0208","DOIUrl":"https://doi.org/10.15275/rusomj.2024.0208","url":null,"abstract":"Background — Gastrointestinal (GI) medications are among the most common drugs used in old age. Recent studies reported heterogeneous association between proton pump inhibitors (PPIs) with neurological complications such as memory impairment. Objective — This research was conducted to assess the effect of different categories of GI drugs on cognitive function of older adults. Methods — This case-control study was carried-out on adults 60 or more years of age and over living in Amirkola, northern Iran, where health-related data were available in the database of the second phase of the Amirkola Health and Ageing Cohort Project (AHAP). Cognitive function of the elderly was examined with the Mini-Mental State Examination (MMSE) screening test. Results — Among 900 examined individuals, total intake of GI medications exhibited no significant effect on cognitive function of older adults [adjusted OR:1.049 (95% CI: 0.757-1.452); p=0.775]; however, a statistically significant effects of PPIs [adjusted OR=1.571 (1.001-2.467); p=0.050) and medicinal drugs affecting GI tract movements [adjusted OR=2.202 (1.180-4.111); p=0.013) on cognitive function were observed. Conclusion — Although total intake of GI medications did not cause a statistically significant impact on cognitive function of older adults, PPIs and medicinal drugs affecting GI tract movements had a significant effect.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"13 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://romj.org/files/pdf/2024/romj-2024-0208.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tools and methods to improve emergency planning for floods","authors":"Lumbroso, Darren","doi":"10.21954/ou.ro.00099500","DOIUrl":"https://doi.org/10.21954/ou.ro.00099500","url":null,"abstract":"The aim of this research was to address how emergency planning for floods and dams can be improved to reduce loss of life. The thesis comprises nine publications. Each publication addresses research questions which feed into the overarching aim. Publications 1 and 2 review historic flood events which occurred in England in 1953 and in France in 2010, leading to a significant loss of life, partly because of deficiencies in emergency planning. Publications 3, 4 and 5 assess how effective emergency plans for floods can reduce fatalities. Publication 3 consolidates lessons learnt from two low-income countries, Bangladesh and Cuba, where there has been an appreciable decrease in deaths from coastal surges and how these are relevant to high-income countries such as the USA. A review of the methods and tools available to increase the effectiveness of emergency plans for floods is provided in Publication 5. Publication 6 follows on from one of the overarching findings of Publications 1 to 5, which is that there is a requirement for a method via which emergency plans for floods can be assessed and improved. Publication 6 details three cases studies in England, France and The Netherlands where a method was developed and applied to assess and to improve emergency plans for floods. Papers 7, 8 and 9 describe how an agent-based model, which represents the dynamic interaction of people, vehicles and buildings with the floodwater or mudflows, can be used to investigate different emergency planning scenarios in order to minimise the potential loss of life from different sources of flooding including dam failures. These publications show how the use of agent-based models, to investigate the emergency management options for floods, allows a counterfactual analysis to be easily undertaken. Counterfactual analysis is increasingly used in the field of emergency management because it enables emergency planners to show causal relationships between interventions. This allows interventions such as increasing the number of safe havens, use of new evacuation routes and improved early warnings to be compared with the existing situation, in the context of the risks that floods pose to people. The research work detailed in the nine publications has informed both policy and practice. For example, Publication 3 helped to inform the US government’s policy on increasing the resilience of vulnerable coastal in the USA to floods. The agent-based model, described in Papers 7, 8 and 9, has been used to inform emergency plans for dams in Australia, Canada, Italy, Japan, Malaysia, Peru and the USA. Overall, this series of publications points towards the need for continuous improvements in the emergency planning for low probability - high consequence flood events and the part agent-based models can play in this.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147914342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-134 And MiR-106b Are Circulating Biomarkers For Temporal Lobe Epilepsy: Pilot Study Results","authors":"Kristina D. Lysova, Anna A. Usoltseva, Ekaterina A. Domoratskaya, Irina G. Areshkina, Natalia A. Shnayder, Diana V. Dmitrenko","doi":"10.15275/rusomj.2023.0303","DOIUrl":"https://doi.org/10.15275/rusomj.2023.0303","url":null,"abstract":"Temporal lobe epilepsy (TLE) is among the most common forms of focal epilepsy in adults. Currently, scientists search for microRNAs as noninvasive epilepsy biomarkers. MicroRNAs constitute a class of short (or small) non-coding RNAs that control the level of gene expression affecting the stability of mRNA. They are key regulators and therapeutic targets in epilepsy. Considering the role of miRNA-134 and miRNA-106b in the processes of epileptogenesis, the goal of our study was the clinical evaluation of their circulation as novel noninvasive molecular diagnostic markers of TLE. Material and Methods — Our pilot study involved 59 participants. The main group included 33 patients with mesial temporal lobe epilepsy, the control group encompassed 26 healthy volunteers. The ranking of patients was carried out depending on the disease duration, presence of epileptiform activity on the electroencephalogram (EEG) and hippocampal sclerosis on MRI, the number of taken antiepileptic drugs (AEDs), and patient response to the pharmacotherapy of epilepsy. The isolation of circulating microRNAs from blood plasma was accomplished via the sorption method, and the analysis of microRNA expression was performed by real-time PCR. Results — The expression levels of miR-134 and miR-106b in blood plasma in patients with TLE were reduced. Therefore, these microRNAs can be diagnostic biomarkers of patients with TLE, compared with the control group. The results of receiver operating characteristic (ROC) analysis yielded high sensitivity and specificity values of this biomarker for the diagnosis of TLE. Conclusion — Circulating miR-134 and miR-106b concentrations were significantly reduced in patients with mesial TLE (MTLE), compared with healthy controls. At the same time, the level of microRNA expression did not depend on the presence of hippocampal sclerosis and the response to antiepileptic therapy.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135385154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood-Derived Exosomes With Anti-Inflammatory Properties As A New Minimally Invasive Intratesticular Therapy For Aflatoxin B1-Associated Chronic Testopathy","authors":"Fatemeh Mansouri Torghabeh, Mona Keivan, Mandana Fakoor, Reza Dadfar, Mahsa Nazarzadeh, Amir Abdolmaleki","doi":"10.15275/rusomj.2023.0301","DOIUrl":"https://doi.org/10.15275/rusomj.2023.0301","url":null,"abstract":"Rationale — Aflatoxin B1 (AB1) is a dangerous toxin causing severe poisoning and even death in human. Testopathy or even infertility is one of the adverse effects of AB1. Exosomes (Ex) with anti-inflammatory action are known as biological substances derived from body fluids. This experimental study aimed to investigate the possible therapeutic effects of blood-derived Ex on orchitis caused by chronic exposure to AB1. Methods — Male rats (n=18) were distributed among three groups: control, AB1 and AB1+Ex. Orchitis was induced with AB1 (45 μL/kg of rat weight) for 40 days (3 times a week). A day later, 10 IU Ex were injected intratesticularly. A week later, testicular tissue and blood serum were sampled. To confirm the therapeutic effects of Ex, inflammatory genes (TNF-α, IL-6 and IL-10), nitric oxide (NO), malondialdehyde (MDA), testosterone (Tes) and sperm quality were fully evaluated. In addition, H&amp;E staining was used to investigate tissue changes, and immunohistochemical (IHC) assessment was employed to detect the inflammatory protein, galectin-3. The results were analyzed using SPSS (v.19). Results and Discussion — Our results confirmed that Ex therapy can significantly (p<0.05) increase normal morphology (25.01±2.23 in the AB1+Ex group vs. 10.1±0.9 in the AB1 group), number (76.12±6.7 in the AB1+Ex group vs. 27.8±3.2 in AB1 group), and vitality (53.4±6.12 in AB1+Ex vs. 41.9±4.6 in AB1) of sperm cells. Blood-derived Ex significantly (p<0.05) reduced the expression of inflammatory genes (6-fold change was observed in AB1+Ex vs. 17-fold in AB1 for TNF-α, 3-fold change was detected in AB1+Ex vs. 6-fold in AB1 for IL-6, and 2-fold change took place in AB1+Ex vs. 6-fold in AB1 for IL-10), NO (23.1±2.6 in AB1+Ex vs. 62.8±8 0.1 in AB1) and MDA (33.2±4.9 in AB1+Ex vs. 68.9±5.46 in AB1), and increased Tes level (7.1±0.5 in AB1+Ex vs. 1.14±0.3 in AB1) in AB1-affected rats. Histopathological evaluations revealed tissue regeneration after Ex injection. Also, the produced sperm cells were of high quality. Conclusion — Intratesticular injection of Ex from the blood can be considered as a novel anti-inflammatory therapy after AB1-induced orchitis.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135420312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Choosing Data Clustering Tools For GIS-Based Visualization Of Disease Incidence In The Population","authors":"Roman V. Buzinov, Vladimir N. Fedorov, Aleksandr A. Kovshov, Yuliya A. Novikova, Nadezhda A. Tikhonova, Maxim S. Petrov, Ksenia V. Krutskaya","doi":"10.15275/rusomj.2023.0306","DOIUrl":"https://doi.org/10.15275/rusomj.2023.0306","url":null,"abstract":"Objective — To substantiate the choice of optimal tools for clustering spatially referenced data on disease incidence for GIS-based analysis of their spatial distribution. Material and Methods — We used primary data on the incidence of malignant neoplasms, chronic alcoholism, and asthma in the population of eight administrative areas in Arkhangelsk Oblast as a constituent entity of the Arctic Zone of the Russian Federation. Disease incidence was averaged over a 5-year period from 2016 to 2020. We assessed the methods for visualizing the distribution of spatially referenced indicators using the ArcMap geoinformation system tools. Results — The study yielded differences in the outcomes of automated clustering of spatially referenced data in ArcMap, depending on the normality of the distribution in individual samples and the spread of indicator values, which was visually reflected on the resulting map. The parameter values in the samples directly affected the features of data clustering. Hence, this issue is important to consider for ensuring the correct choice of the appropriate analytical tool. Conclusion — Our study demonstrated that when using tools for automated clustering of spatially referenced incidence data in terms of their visualization in ArcGIS, it is necessary to consider the factors that directly affect the accuracy of their presentation. We consider it most appropriate to use a clustering tool based on the geometric interval method.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135420130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy Of Vibroacoustic Therapy In Treatment Of Pneumonia In Children With Bronchopulmonary Dysplasia","authors":"Madina Syzdykova, Marina Morenko, Madina Gatauova, Saule Zhumambayeva, Kseniya Shnaider","doi":"10.15275/rusomj.2023.0307","DOIUrl":"https://doi.org/10.15275/rusomj.2023.0307","url":null,"abstract":"Background — Advances in neonatal intensive care have resulted in high survival rates for infants born with extremely low birth weight. Accordingly, the treatment and rehabilitation of premature neonates with bronchopulmonary dysplasia (BPD) is indisputably an urgent problem. Objective — To evaluate the efficacy of vibroacoustic therapy in patients with BPD in the complex of therapeutic measures. Material and Methods — The study was a prospective, non-randomized open-label controlled clinical trial conducted in 2018-2020 in Astana, Kazakhstan. It included 69 children with BPD hospitalized with a concomitant diagnosis of pneumonia between the ages of 1 and 2 years. The study subjects were distributed among two groups: Group 1 (the main group, n=37) receiving vibroacoustic therapy with the VibroLung apparatus in combination with the main treatment and Group 2 (the control, n=32) receiving solely the main treatment. Results — The inclusion of the vibroacoustic method of treatment in the combination therapy of pneumonia in children with BPD resulted in early leveling of the following clinical symptoms: a reduction in the duration of the high fever by 1.18 days vs. the control group (p<0.05), relief of respiratory failure 1.4 days earlier vs. the control group (p<0.05) and a decrease in the duration of inpatient treatment by 2.9 days, compared with the control group (p<0.05). All of these implied the efficacy of this treatment method. Conclusion — Our results demonstrated that vibroacoustic therapy was a relevant addition to the treatment of pneumonia in children with BPD.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135420973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardioprotective Dietary Pattern Of Siberian Population","authors":"Darya P. Tsygankova, Evgeny D. Bazdyrev, Alena S. Agienko, Olga V. Nakhratova, Elena V. Indukaeva, Galina V. Artamonova, Olga L. Barbarash","doi":"10.15275/rusomj.2023.0302","DOIUrl":"https://doi.org/10.15275/rusomj.2023.0302","url":null,"abstract":"Background — Analysis of eating habits can help identify cardioprotective dietary patterns. It is necessary to qualitatively study the diet of modern inhabitants of Siberia and identify food stereotypes that contribute to effective cardiac prophylaxis. Objective — to study the diet of the inhabitants of Siberia and to identify the features of cardioprotective nutrition. Methods — A clinical and epidemiological prospective group study of the population permanently residing in Kemerovo Oblast (administrative entity of the Russian Federation) was carried out. The baseline study included 1,124 women (70.3%) and 476 men (29.7%). To identify latent factors (stereotypes of eating behavior), we used factor analysis (method of principal components). Results — Adherence to the fruit-and-vegetable stereotype was associated with an increased risk of obesity according to body mass index (BMI) (OR=1.57, CI: 1.27-1.96), waist circumference (WC) (OR=1.43, CI: 1.1-1.9), and presence of diabetes mellitus (DM) (OR=1.27, CI: 1.2-2.2). Adherence to the protein-and-carbohydrate dietary pattern was connected to a reduced risk of detecting obesity in terms of BMI criteria (OR=0.75, CI: 0.6-0.95, p=0.015), WC (OR=0.52, CI:0.41-0.66), DM (OR=0.66, CI:0.47-0.93), hypercholesterolemia (OR=0.78, CI:0.62-0.98) and hypertriglyceridemia (OR=0.66, CI:0.52-0.83). Prospective observation demonstrated the variability of stereotypes: after three years, the following five stereotypes were identified: vegetable, protein-and-carbohydrate, fruit, dairy, and mixed. Conclusion — In contrast to the protein-and-carbohydrate diet, the fruit-and-vegetable stereotype of nutrition was associated with the development of obesity and DM. Considering the obtained results, it is necessary to study the qualitative characteristics of each stereotype (the content of macro- and microelements, kcal) and the motor activity of the respondents.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135420774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthovoltage X-Ray Therapy Significantly Reduces Disability Risk In Knee Osteoarthritis Patients: A Decade-Long Cohort Study","authors":"Maria V. Makarova, Mikhail Yu. Valkov, Andrey M. Grjibovski","doi":"10.15275/rusomj.2023.0304","DOIUrl":"https://doi.org/10.15275/rusomj.2023.0304","url":null,"abstract":"Introduction — Osteoarthritis (OA) of the knee and hip joints affects 13% of the adult population in the Russian Federation. While medications can provide some relief from the pain associated with OA, they are often not enough. An alternative treatment option is orthovoltage radiation therapy (OVRT), which not only relieves pain, but can also help prevent disability. However, there is little evidence for the long-term effectiveness of OVRT. Objective — We compared the incidence of disability among patients with OA who received standard treatment with nonsteroidal anti-inflammatory drugs (NSAIDs) in combination with the symptomatic slow-acting drugs for osteoarthritis (SYSADOA), or in combination with OVRT for knee OA in the setting of an open randomized controlled trial with long-term follow-up. Material and Methods — The sampling frame included patients with confirmed OA of the knee sensu Altman (1991), with radiographic grades of OA from 0 to 2 sensu Kellgren-Lawrence. A total of 292 patients were randomly distributed among two groups of equal sizes. The control group received combination therapy with NSAIDs and SYSADOA. In the experimental group, OVRT was additionally performed at a total dose of 4.5 Gy. Relationships between treatment regimen and time to disability were studied using actuarial analysis, Kaplan-Meier plots. Crude and adjusted hazard ratios (HR) with 95% confidence intervals (CI), as well as attributable fraction (AF) and population attributable fraction (PAF) were calculated. Results — The cumulative time at risk for disability was 2,304.9 person-years. In total, 9.5% of patients in the experimental group became disabled during the observation period vs. 17.8% in the control group. In the experimental group, the level of disability was lower (HR=0.49, 95% CI: 0.26-0.95). Differences became more pronounced after adjusting for sex, age, radiographic grade of OA, pain intensity, and duration of OA before treatment (HR=0.24, 95% CI: 0.11-0.48). AF and PAF were 49.9% and 25.8%, respectively. Conclusion — It has been shown that the introduction of OVRT in the treatment regimen can reduce the disability of patients with knee OA by almost 50%. One in four disability cases could be prevented if OVRT were used universally in the treatment of knee OA. Our results indicate that combining OVRT with standard care is a more effective approach to preventing disability in patients with knee OA than standard treatment alone.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135420588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}