MiR-134和MiR-106b是颞叶癫痫的循环生物标志物:初步研究结果

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
Kristina D. Lysova, Anna A. Usoltseva, Ekaterina A. Domoratskaya, Irina G. Areshkina, Natalia A. Shnayder, Diana V. Dmitrenko
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引用次数: 0

摘要

颞叶癫痫(TLE)是成人局灶性癫痫最常见的形式之一。目前,科学家们正在寻找microrna作为非侵入性癫痫的生物标志物。MicroRNAs是一类短的(或小的)非编码rna,控制着影响mRNA稳定性的基因表达水平。它们是癫痫的关键调节因子和治疗靶点。考虑到miRNA-134和miRNA-106b在癫痫发生过程中的作用,我们的研究目的是临床评估它们的循环作为TLE的新型无创分子诊断标志物。材料和方法:我们的初步研究涉及59名参与者。主要组为33例内侧颞叶癫痫患者,对照组为26例健康志愿者。根据病程、脑电图(EEG)上癫痫样活动和MRI上海马硬化症的存在、服用抗癫痫药物(aed)的数量以及患者对癫痫药物治疗的反应对患者进行排名。采用吸附法分离血浆中循环microRNA, real-time PCR分析microRNA表达。结果- TLE患者血浆中miR-134和miR-106b的表达水平降低。因此,与对照组相比,这些microrna可以作为TLE患者的诊断性生物标志物。受试者工作特征(ROC)分析结果显示,该生物标志物对TLE的诊断具有很高的敏感性和特异性。结论:与健康对照相比,中膜TLE (MTLE)患者的循环miR-134和miR-106b浓度显著降低。同时,microRNA的表达水平不依赖于海马硬化的存在和抗癫痫治疗的反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MiR-134 And MiR-106b Are Circulating Biomarkers For Temporal Lobe Epilepsy: Pilot Study Results
Temporal lobe epilepsy (TLE) is among the most common forms of focal epilepsy in adults. Currently, scientists search for microRNAs as noninvasive epilepsy biomarkers. MicroRNAs constitute a class of short (or small) non-coding RNAs that control the level of gene expression affecting the stability of mRNA. They are key regulators and therapeutic targets in epilepsy. Considering the role of miRNA-134 and miRNA-106b in the processes of epileptogenesis, the goal of our study was the clinical evaluation of their circulation as novel noninvasive molecular diagnostic markers of TLE. Material and Methods — Our pilot study involved 59 participants. The main group included 33 patients with mesial temporal lobe epilepsy, the control group encompassed 26 healthy volunteers. The ranking of patients was carried out depending on the disease duration, presence of epileptiform activity on the electroencephalogram (EEG) and hippocampal sclerosis on MRI, the number of taken antiepileptic drugs (AEDs), and patient response to the pharmacotherapy of epilepsy. The isolation of circulating microRNAs from blood plasma was accomplished via the sorption method, and the analysis of microRNA expression was performed by real-time PCR. Results — The expression levels of miR-134 and miR-106b in blood plasma in patients with TLE were reduced. Therefore, these microRNAs can be diagnostic biomarkers of patients with TLE, compared with the control group. The results of receiver operating characteristic (ROC) analysis yielded high sensitivity and specificity values of this biomarker for the diagnosis of TLE. Conclusion — Circulating miR-134 and miR-106b concentrations were significantly reduced in patients with mesial TLE (MTLE), compared with healthy controls. At the same time, the level of microRNA expression did not depend on the presence of hippocampal sclerosis and the response to antiepileptic therapy.
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来源期刊
Russian Open Medical Journal
Russian Open Medical Journal MEDICINE, GENERAL & INTERNAL-
CiteScore
0.90
自引率
0.00%
发文量
39
期刊介绍: Russian Open Medical Journal (RusOMJ) (ISSN 2304-3415) is an international peer reviewed open access e-journal. The website is updated quarterly with the RusOMJ’s latest original research, clinical studies, case reports, reviews, news, and comment articles. This Journal devoted to all field of medicine. All the RusOMJ’s articles are published in full on www.romj.org with open access and no limits on word counts. Our mission is to lead the debate on health and to engage, inform, and stimulate doctors, researchers, and other health professionals in ways that will improve outcomes for patients. The RusOMJ team is based mainly in Saratov (Russia), although we also have editors elsewhere in Russian and in other countries.
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