Revista Iberoamericana De Micologia最新文献_第4页

In vitro activity of juglone (5-hydroxy-1,4-naphthoquinone) against both fluconazole-resistant and susceptible Candida isolates 核桃酮(5-羟基-1,4-萘醌)对氟康唑耐药和敏感念珠菌的体外活性研究

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-04-01 DOI: 10.1016/j.riam.2022.01.004

Afsane Vaezi , Masoud Moghadaszadeh , Elahe Nasri , Shima Gharibi , Kambiz Diba , Adam Matkowski , Hamed Fakhim

{"title":"In vitro activity of juglone (5-hydroxy-1,4-naphthoquinone) against both fluconazole-resistant and susceptible Candida isolates","authors":"Afsane Vaezi , Masoud Moghadaszadeh , Elahe Nasri , Shima Gharibi , Kambiz Diba , Adam Matkowski , Hamed Fakhim","doi":"10.1016/j.riam.2022.01.004","DOIUrl":"10.1016/j.riam.2022.01.004","url":null,"abstract":"<div><h3>Background</h3><p>The rise in antifungal resistance and drug class limitations are causing higher morbidity and mortality rates all over the world. This issue highlights the urgent need for new and improved antifungal drugs with a novel target.</p></div><div><h3>Aims</h3><p>In order to evaluate whether juglone can be served as an alternative antifungal to cure drug-resistant <em>Candida</em> infections, we studied the <em>in vitro</em> susceptibility of juglone against fluconazole-susceptible and -resistance <em>Candida</em> isolates, alone and in combination.</p></div><div><h3>Methods</h3><p>Antifungal susceptibility testing was performed according to the CLSI (Clinical and Laboratory Standards Institute) guidelines.</p></div><div><h3>Results</h3><p>Juglone exhibited the highest minimal inhibitory concentration (MIC) values, followed by fluconazole and nystatin. Voriconazole showed significantly better antifungal activity than juglone, fluconazole, and nystatin, with MIC<sub>50</sub> and MIC<sub>90</sub> of 0.031 and 0.5μg/mL. There were significant differences in MICs of fluconazole (<em>p</em>  <0.0003) between <em>Candida</em> <em>albicans</em> and the rest of the species. Combination of juglone with fluconazole revealed insignificant effects against fluconazole-susceptible and -resistant <em>Candida</em> isolates. Juglone increased the antifungal activity of fluconazole; however, no synergism effects were observed for any combination, and only an insignificant effect was found against all tested <em>Candida</em> species.</p></div><div><h3>Conclusions</h3><p>Although obtaining new antifungal drugs is a critical point, a completely novel approach should be implemented.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 2","pages":"Pages 50-53"},"PeriodicalIF":1.9,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48221145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of the diagnostic accuracy of galactomannan from the bronchoalveolar lavage fluid of patients with suspected invasive pulmonary aspergillosis 支气管肺泡灌洗液半乳甘露聚糖诊断疑似侵袭性肺曲霉病的准确性评价

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-04-01 DOI: 10.1016/j.riam.2021.07.002

Rafaela Manzoni Bernardi , Sizuane Rieger Holler , Ellis Gabriela Correa de Almeida , Camila Anton , Felipe Dominguez Machado , Denise Rossato Silva

{"title":"Evaluation of the diagnostic accuracy of galactomannan from the bronchoalveolar lavage fluid of patients with suspected invasive pulmonary aspergillosis","authors":"Rafaela Manzoni Bernardi , Sizuane Rieger Holler , Ellis Gabriela Correa de Almeida , Camila Anton , Felipe Dominguez Machado , Denise Rossato Silva","doi":"10.1016/j.riam.2021.07.002","DOIUrl":"10.1016/j.riam.2021.07.002","url":null,"abstract":"<div><h3>Background</h3><p>Several studies to evaluate the accuracy of galactomannan (GM) in bronchoalveolar lavage fluid (BALF) as a diagnostic tool have been carried out; however, there are still controversies about the optimal cut-off point of BALF GM.</p></div><div><h3>Aims</h3><p>The objective of this study was to determine the diagnostic accuracy and the optimal cut-off point on BALF GM from patients with suspected invasive pulmonary aspergillosis (IPA) in a tertiary care hospital.</p></div><div><h3>Methods</h3><p>A cross-sectional study with 188 patients (≥18 years) that had undergone a bronchoscopy with BAL due to suspected IPA was carried out. IPA was diagnosed according to the EORTC/MSG guidelines.</p></div><div><h3>Results</h3><p>The optimal optical density cut-off point for BALF GM was 0.67, with sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 70%, 32.3%, and 100%, respectively.</p></div><div><h3>Conclusions</h3><p>BALF GM detection proved to be a useful supplementary technique in the early diagnosis of IPA in both neutropenic and non-neutropenic patients.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 2","pages":"Pages 31-35"},"PeriodicalIF":1.9,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43000411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible Trichosporon asahii urinary tract infection in a critically ill COVID-19 patient COVID-19危重症患者可能的朝日毛孢丝状体尿路感染

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-04-01 DOI: 10.1016/j.riam.2022.05.001

Marco Vianello , Daniel F.F. de Jesus , Jorge M. Sampaio , Guilherme M. de Oliveira , Nilton Lincopan , Kelly Ishida

{"title":"Possible Trichosporon asahii urinary tract infection in a critically ill COVID-19 patient","authors":"Marco Vianello , Daniel F.F. de Jesus , Jorge M. Sampaio , Guilherme M. de Oliveira , Nilton Lincopan , Kelly Ishida","doi":"10.1016/j.riam.2022.05.001","DOIUrl":"10.1016/j.riam.2022.05.001","url":null,"abstract":"<div><h3>Background</h3><p><em>Trichosporon asahii</em>, an emerging fungal pathogen, has been frequently associated with invasive infections in critically ill patients.</p></div><div><h3>Case report</h3><p>A 74-year-old male patient diagnosed with COVID-19 was admitted to an Intensive Care Unit (ICU). During hospitalization, the patient displayed episodes of bacteremia by <em>Staphylococcus haemolyticus</em> and a possible urinary tract infection by <em>T. asahii</em>. While the bacterial infection was successfully treated using broad-spectrum antibiotics, the fungal infection in the urinary tract was unsuccessfully treated with anidulafungin and persisted until the patient died.</p></div><div><h3>Conclusions</h3><p>With the evolving COVID-19 pandemic, invasive fungal infections have been increasingly reported, mainly after taking immunosuppressant drugs associated with long-term broad-spectrum antibiotic therapy. Although <em>Candida</em> and <em>Aspergillus</em> are still the most prevalent invasive fungi, <em>T. asahii</em> and other agents have emerged in critically ill patients. Therefore, a proper surveillance and diagnosing any fungal infection are paramount, particularly in COVID-19 immunocompromised populations.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 2","pages":"Pages 54-56"},"PeriodicalIF":1.9,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40471897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epizootic lymphangitis: A neglected disease of working equids 兽疫性淋巴管炎:一种被劳动马匹忽视的疾病

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.11.001

F. Javier Cabañes

引用次数: 0

Lodderomyces elongisporus: ¿agente causal de vaginitis? 长孢Lodderomyces elongisporus:阴道炎的病原体?

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.11.002

Laura Sante , Patricia Capón , Amparo Coira Nieto , Pilar Alonso-García

引用次数: 1

Ángela Restrepo Moreno: The wise woman par excellence! 莫雷诺:The wiseÁngela雷斯特雷波出类拔萃的女人!

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2022.03.001

引用次数: 0

Estado actual de la histoplasmosis diseminada progresiva en pacientes con infección por el VIH en un hospital de tercer nivel en Perú 秘鲁一家三级医院艾滋病毒感染患者进行性传播组织胞浆菌病的现状

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.10.001

Omayra Chincha , Beatriz Bustamante

{"title":"Estado actual de la histoplasmosis diseminada progresiva en pacientes con infección por el VIH en un hospital de tercer nivel en Perú","authors":"Omayra Chincha , Beatriz Bustamante","doi":"10.1016/j.riam.2021.10.001","DOIUrl":"https://doi.org/10.1016/j.riam.2021.10.001","url":null,"abstract":"<div><h3>Background</h3><p>Progressive disseminated histoplasmosis (PDH) is an endemic disease in most of Latin America, especially among patients with HIV. There are few reports about this disease in Peru.</p></div><div><h3>Aims</h3><p>To describe the clinical, epidemiological and mycological features of patients with PDH and HIV evaluated in a tertiary hospital.</p></div><div><h3>Methods</h3><p>A retrospective study to find out the data of patients diagnosed with PDH and HIV in the period 2000–2019 was carried out. For the statistical analysis of quantitative variables, measures of central tendency and dispersion were used; for the qualitative variables, absolute and relative frequencies were used.</p></div><div><h3>Results</h3><p>Forty-three male patients with PDH were diagnosed in the study period, with a median age of 33 years (IQR: 29–38 years) and a median CD<sub>4</sub> lymphocytes count of 39cells/mm<sup>3</sup> (IQR: 20–83 cells/mm<sup>3</sup>). Eighty six percent of the patients were born or had travelled to the jungle, 58.1% were alcohol users and 16.1% had a history of pulmonary tuberculosis. When compared to histopathology, the culture had a better sensitivity to achieve a diagnosis (<em>p</em>   <!-->cells/mm<sup>3</sup>. In patients with the described characteristics it would be advisable to check for PDH. Implementing rapid diagnostic tests is also necessary.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 1","pages":"Pages 25-27"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92219040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Unusual form of histoplasmosis in dogs and cats 狗和猫的组织胞浆菌病的不寻常形式

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.09.001

F. Javier Cabañes

引用次数: 0

Potencial de la levadura oleaginosa Clavispora lusitaniae Hi2 en la conversión de residuos agroindustriales a lípidos 油料酵母lusitaniae Hi2在农用工业废弃物转化为脂类方面的潜力

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.07.001

Aime Frida Moran-Mejía , Rosa Angélica Guillén-Garcés , Gabriela Eleonora Moeller-Chávez , Jesús Hernández-Romano , María Inés Chávez-Béjar , Clarita Olvera-Carranza , Luis Gerardo Treviño-Quintanilla

{"title":"Potencial de la levadura oleaginosa Clavispora lusitaniae Hi2 en la conversión de residuos agroindustriales a lípidos","authors":"Aime Frida Moran-Mejía , Rosa Angélica Guillén-Garcés , Gabriela Eleonora Moeller-Chávez , Jesús Hernández-Romano , María Inés Chávez-Béjar , Clarita Olvera-Carranza , Luis Gerardo Treviño-Quintanilla","doi":"10.1016/j.riam.2021.07.001","DOIUrl":"https://doi.org/10.1016/j.riam.2021.07.001","url":null,"abstract":"<div><h3>Background</h3><p>Single-cell oils obtained from oleaginous microorganisms by using lignocellulosic waste hydrolysates are an alternative for producing biodiesel.</p></div><div><h3>Aims</h3><p>To isolate a yeast strain able to produce lipids from centrifuged nejayote (CN), hydrolyzed nejayote solids (HNS) and hydrolyzed sugarcane bagasse (HSB).</p></div><div><h3>Methods</h3><p>In order to identify the yeasts recovered, 26S ribosomal DNA was sequenced. The metabolic profile was assessed by using API20C AUX strips. The nutritional characterization of CN, HNS and HSB was performed by quantifying reducing sugars, total carbohydrates, starch, protein and total nitrogen. The biomass and lipid production ability were evaluated by performing growth kinetics of <em>Clavispora lusitaniae</em> Hi2 in combined culture media.</p></div><div><h3>Results</h3><p>Six oleaginous yeast strains were isolated and identified, selecting <em>C. lusitaniae</em> Hi2 to study its lipids production by using nejayote. The <em>C. lusitaniae</em> Hi2 strain can use glucose, xylose, arabinose, galactose and cellobiose as carbon sources. Cultures of <em>C. lusitaniae</em> Hi2 presented the best biomass (5.6<span>±</span>0.28 g/L) and lipid production (0.99<span>±</span>0.09 g/L) at 20 h of incubation with the CN:HNS media in the 25:75 and 50:50 ratios, respectively.</p></div><div><h3>Conclusions</h3><p>The use of CN, HNS and HSB for the growth of <em>C. lusitaniae</em> Hi2 is an option to take advantage of these agro-industrial residues and generate compounds of biotechnological interest.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 1","pages":"Pages 6-15"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92266552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Lethal destructive sinusopathy due to amphotericin B-resistant Aspergillus flavus: A case report 两性霉素b耐药黄曲霉致致致命性破坏性鼻窦病1例。

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2022.01.001

Tiago Luiz Lagedo Ferraz , Eduardo Marques Araújo , Rodolfo Froes Calixto , Monique Lima Martins Sampaio , Luciana Rezende Bandeira de Mello , Kaliny Benicio Torres , Rossana de Aguiar Cordeiro , Patrice Le Pape , Reginaldo Gonçalves de Lima-Neto

{"title":"Lethal destructive sinusopathy due to amphotericin B-resistant Aspergillus flavus: A case report","authors":"Tiago Luiz Lagedo Ferraz , Eduardo Marques Araújo , Rodolfo Froes Calixto , Monique Lima Martins Sampaio , Luciana Rezende Bandeira de Mello , Kaliny Benicio Torres , Rossana de Aguiar Cordeiro , Patrice Le Pape , Reginaldo Gonçalves de Lima-Neto","doi":"10.1016/j.riam.2022.01.001","DOIUrl":"10.1016/j.riam.2022.01.001","url":null,"abstract":"<div><h3>Background</h3><p>The prevalence of pulmonary aspergillosis and the importance of its early diagnosis are recognized. However, non-pulmonary involvement, including the sinuses region, is not frequently reported, and an infection in this area can affect all paranasal sinuses (pansinusopathy), being a rare pathology that affects immunocompromised hosts. Recent studies have highlighted the occurrence of <span><em>Aspergillus</em><em> flavus</em></span> resistant to antifungal therapy. Therefore, a nasal sinus infection by resistant <em>Aspergillus</em> strains in immunocompromised patients may be linked to a high risk of lethality.</p></div><div><h3>Case report</h3><p>We are reporting a resistant <em>A. flavus</em> infection in an allogeneic hematopoietic stem cell transplant recipient with episodes of febrile neutropenia, and prolonged use of various antibacterial drugs and antifungal prophylaxis. The patient underwent brain magnetic resonance, which showed the presence of pansinusopathy, and presented necrosis in the left nasal region. Direct microscopic examination of a sample taken from the nasal mucosa revealed the presence of septate hyphae and conidiophores resembling those of <em>A. flavus</em><span>, that species being the identification achieved with MALDI-TOF MS. Antifungigram was performed by microdilution in broth (EUCAST-E.DEF. 9.3.2) and E-test, and resistance to amphotericin B was shown in both tests. The patient died after septic shock and hemorrhage.</span></p></div><div><h3>Conclusions</h3><p>Invasive fungal infections due to amphotericin-B resistant <em>A. flavus</em> may lead to the death of the patient due to an ineffective therapeutic management. Therefore, antifungal susceptibility testing are of utmost importance for administering the proper treatment.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 1","pages":"Pages 21-24"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42474513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3