Revista Iberoamericana De Micologia最新文献

{"title":"Phaeohyphomycosis caused by Neoscytalidium dimidiatum in a COVID-19 patient","authors":"Somayeh Dolatabadi ,&nbsp;Sara Nasirharandi ,&nbsp;Morteza Pourahmad ,&nbsp;Kazem Ahmadikia ,&nbsp;Mojgan Mokhtari ,&nbsp;Mohammad Javad Najafzadeh ,&nbsp;Rasoul Mohammadi","doi":"10.1016/j.riam.2022.07.002","DOIUrl":"10.1016/j.riam.2022.07.002","url":null,"abstract":"<div><h3>Background</h3><p><em>Neoscytalidium</em> <em>dimidiatum</em><span> is an opportunistic dematiaceous fungus belonging to the class Dothideomycetes.</span></p></div><div><h3>Case report</h3><p>We report a case of <em>N. dimidiatum</em> cerebral phaeohyphomycosis post COVID-19 infection in a 32-year-old male from Iran. The causative agent was identified by cytopathology, routine mycological methods, and DNA sequencing of the internal transcribed spacer (ITS) region of rDNA. Apart from COVID-19 complications and the corticosteroid therapy, no underlying condition was diagnosed. The symptoms suggesting the fungal infection were shown two weeks after being discharged from COVID-19 hospital stay. Magnetic resonance of the brain showed a multi-focal central nervous system infection. The delayed identification of the fungus and, thus, a late starting of the antifungal treatment with amphotericin B, might have affected the patient outcome as he finally died.</p></div><div><h3>Conclusions</h3><p>Considering the rare incidence of <em>N. dimidiatum</em> infections, this case should aware us about them, leading to a timely antifungal management.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 3","pages":"Pages 72-76"},"PeriodicalIF":1.9,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10381155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Candida albicans and Staphylococcus aureus polymicrobial biofilm on different surfaces","authors":"Eduardo Hernandez-Cuellar ,&nbsp;Alma Lilián Guerrero-Barrera ,&nbsp;Francisco Javier Avelar-Gonzalez ,&nbsp;Juan Manuel Díaz ,&nbsp;Alfredo Salazar de Santiago ,&nbsp;Jesús Chávez-Reyes ,&nbsp;Emanuel Poblano-Sánchez","doi":"10.1016/j.riam.2022.04.001","DOIUrl":"10.1016/j.riam.2022.04.001","url":null,"abstract":"<div><h3>Background</h3><p><em>Staphylococcus aureus</em> and <em>Candida albicans</em> have been co-isolated from biofilm-associated diseases such as denture stomatitis, periodontitis, and burn wound infections, as well as from medical devices. However, the polymicrobial biofilm of both microorganisms has not been fully characterized.</p></div><div><h3>Aims</h3><p>To characterize the polymicrobial biofilm of <em>C. albicans</em> and <em>S. aureus</em> in terms of microbial density, synergy, composition, structure, and stability against antimicrobials and chemical agents.</p></div><div><h3>Methods</h3><p>Crystal violet assay was used to measure the biofilm formation. Scanning electron microscopy and confocal microscopy were used to analyze the structure and chemical composition of the biofilms, respectively.</p></div><div><h3>Results</h3><p>Supplemented media with fetal bovine serum (FBS) decreased the biofilm formation of <em>S. aureus</em> and the polymicrobial biofilm. For <em>C. albicans</em>, depending on the culture media, the addition of glucose or FBS had a positive effect in biofilm formation. FBS decreased the adhesion to polystyrene wells for both microorganisms. Supplementing the media with glucose and FBS enhanced the growth of <em>C. albicans</em> and <em>S. aureus</em>, respectively. It seems that <em>C. albicans</em> contributes the most to the adhesion process and to the general structure of the biofilms on all the surfaces tested, including a catheter model. Interestingly, <em>S. aureus</em> showed a great adhesion capacity to the surface of <em>C. albicans</em> in the biofilms. Proteins and β-1,6-linked polysaccharides seem to be the most important molecules in the polymicrobial biofilm.</p></div><div><h3>Conclusions</h3><p>The polymicrobial biofilm had a complex structure, with <em>C. albicans</em> serving as a scaffold where <em>S. aureus</em> adheres, preferentially to the hyphal form of the fungus. Detection of polymicrobial infections and characterization of biofilms will be necessary in the future to provide a better treatment.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 2","pages":"Pages 36-43"},"PeriodicalIF":1.9,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1130140622000171/pdfft?md5=247b42127876dfcc26fdbcfb5390128f&pid=1-s2.0-S1130140622000171-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40333691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saprolegniosis: A reemerging disease","authors":"F. Javier Cabañes","doi":"10.1016/j.riam.2021.12.001","DOIUrl":"10.1016/j.riam.2021.12.001","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 2","pages":"Pages 29-30"},"PeriodicalIF":1.9,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39583279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspergillosis by cryptic Aspergillus species: A case series and review of the literature","authors":"Mariana Fernandez-Pittol ,&nbsp;Izaskun Alejo-Cancho ,&nbsp;Elisa Rubio-García ,&nbsp;Celia Cardozo ,&nbsp;Pedro Puerta-Alcalde ,&nbsp;Estela Moreno-García ,&nbsp;Nicole Garcia-Pouton ,&nbsp;Miriam Garrido ,&nbsp;Miriam Villanueva ,&nbsp;Ana Alastruey-Izquierdo ,&nbsp;Cristina Pitart ,&nbsp;Carolina Garcia-Vidal ,&nbsp;Francesc Marco","doi":"10.1016/j.riam.2022.04.002","DOIUrl":"10.1016/j.riam.2022.04.002","url":null,"abstract":"<div><h3>Background</h3><p>The cryptic <em>Aspegillus</em> species are rare, these microorganisms are usually more resistant to common antifungal therapies. Therefore, a correct identification is important when evaluating the impact of such species in aspergillosis.</p></div><div><h3>Aims</h3><p>We aimed to describe the frequency, clinical and microbiological characteristics, and the outcomes of those cases of aspergillosis caused by cryptic species in a tertiary hospital.</p></div><div><h3>Methods</h3><p>We retrospectively identified all microbiologically documented cases of aspergillosis between January 2013 and December 2018. Definitive species identification of clinically significant isolates was achieved via sequencing methods. The polymerase chain reaction (PCR) products were sequenced, and the results obtained were compared to sequences deposited in GenBank. Antifungal susceptibility testing was performed using the Sensititre® YeastOne® panel.</p></div><div><h3>Results</h3><p>A total of 679 <span><em>Aspergillus</em></span> isolates were recovered from 489 patients, of which 109 were clinically relevant. Ten (9.2%) isolates were identified as cryptic species: <em>Aspergillus arcoverdensis</em> (2), <em>Aspergillus lentulus</em> (2), <em>Aspergillus ellipticus</em> (2), <em>Aspergillus alliaceus</em> (1), <em>Aspergillus nomius</em> (1), <em>Aspergillus tubingensis</em> (1) and <em>Aspergillus montevidensis</em> (1). Most patients already suffered some type of immunosuppression. Half of these patients had required intensive care before the infection showed up, and most of them had a pulmonary infection. Mortality at the 100-day follow-up was 40%. Antifungal susceptibility testing was performed on three of the isolates (<em>A. arcoverdensis</em>, <em>A. tubingensis</em> and <em>A. nomius</em><span>), which showed high minimum inhibitory concentrations (MIC) for azoles and amphotericin B.</span></p></div><div><h3>Conclusions</h3><p>The frequency of cryptic species in our centre was 9.2%. Most patients had some degree of immunosuppression, and the mortality rate was 40%.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 2","pages":"Pages 44-49"},"PeriodicalIF":1.9,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40398060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro activity of juglone (5-hydroxy-1,4-naphthoquinone) against both fluconazole-resistant and susceptible Candida isolates","authors":"Afsane Vaezi ,&nbsp;Masoud Moghadaszadeh ,&nbsp;Elahe Nasri ,&nbsp;Shima Gharibi ,&nbsp;Kambiz Diba ,&nbsp;Adam Matkowski ,&nbsp;Hamed Fakhim","doi":"10.1016/j.riam.2022.01.004","DOIUrl":"10.1016/j.riam.2022.01.004","url":null,"abstract":"<div><h3>Background</h3><p>The rise in antifungal resistance and drug class limitations are causing higher morbidity and mortality rates all over the world. This issue highlights the urgent need for new and improved antifungal drugs with a novel target.</p></div><div><h3>Aims</h3><p>In order to evaluate whether juglone can be served as an alternative antifungal to cure drug-resistant <em>Candida</em> infections, we studied the <em>in vitro</em> susceptibility of juglone against fluconazole-susceptible and -resistance <em>Candida</em> isolates, alone and in combination.</p></div><div><h3>Methods</h3><p>Antifungal susceptibility testing was performed according to the CLSI (Clinical and Laboratory Standards Institute) guidelines.</p></div><div><h3>Results</h3><p>Juglone exhibited the highest minimal inhibitory concentration (MIC) values, followed by fluconazole and nystatin. Voriconazole showed significantly better antifungal activity than juglone, fluconazole, and nystatin, with MIC₅₀ and MIC₉₀ of 0.031 and 0.5<!--> <!-->μg/mL. There were significant differences in MICs of fluconazole (<em>p</em> <!-->&lt;<!--> <!-->0.001) and juglone (<em>p</em> <!-->&lt;<!--> <!-->0.0003) between <em>Candida</em> <em>albicans</em> and the rest of the species. Combination of juglone with fluconazole revealed insignificant effects against fluconazole-susceptible and -resistant <em>Candida</em> isolates. Juglone increased the antifungal activity of fluconazole; however, no synergism effects were observed for any combination, and only an insignificant effect was found against all tested <em>Candida</em> species.</p></div><div><h3>Conclusions</h3><p>Although obtaining new antifungal drugs is a critical point, a completely novel approach should be implemented.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 2","pages":"Pages 50-53"},"PeriodicalIF":1.9,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48221145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the diagnostic accuracy of galactomannan from the bronchoalveolar lavage fluid of patients with suspected invasive pulmonary aspergillosis","authors":"Rafaela Manzoni Bernardi ,&nbsp;Sizuane Rieger Holler ,&nbsp;Ellis Gabriela Correa de Almeida ,&nbsp;Camila Anton ,&nbsp;Felipe Dominguez Machado ,&nbsp;Denise Rossato Silva","doi":"10.1016/j.riam.2021.07.002","DOIUrl":"10.1016/j.riam.2021.07.002","url":null,"abstract":"<div><h3>Background</h3><p>Several studies to evaluate the accuracy of galactomannan (GM) in bronchoalveolar lavage fluid (BALF) as a diagnostic tool have been carried out; however, there are still controversies about the optimal cut-off point of BALF GM.</p></div><div><h3>Aims</h3><p>The objective of this study was to determine the diagnostic accuracy and the optimal cut-off point on BALF GM from patients with suspected invasive pulmonary aspergillosis (IPA) in a tertiary care hospital.</p></div><div><h3>Methods</h3><p>A cross-sectional study with 188 patients (≥18 years) that had undergone a bronchoscopy with BAL due to suspected IPA was carried out. IPA was diagnosed according to the EORTC/MSG guidelines.</p></div><div><h3>Results</h3><p>The optimal optical density cut-off point for BALF GM was 0.67, with sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 70%, 32.3%, and 100%, respectively.</p></div><div><h3>Conclusions</h3><p>BALF GM detection proved to be a useful supplementary technique in the early diagnosis of IPA in both neutropenic and non-neutropenic patients.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 2","pages":"Pages 31-35"},"PeriodicalIF":1.9,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43000411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possible Trichosporon asahii urinary tract infection in a critically ill COVID-19 patient","authors":"Marco Vianello ,&nbsp;Daniel F.F. de Jesus ,&nbsp;Jorge M. Sampaio ,&nbsp;Guilherme M. de Oliveira ,&nbsp;Nilton Lincopan ,&nbsp;Kelly Ishida","doi":"10.1016/j.riam.2022.05.001","DOIUrl":"10.1016/j.riam.2022.05.001","url":null,"abstract":"<div><h3>Background</h3><p><em>Trichosporon asahii</em>, an emerging fungal pathogen, has been frequently associated with invasive infections in critically ill patients.</p></div><div><h3>Case report</h3><p>A 74-year-old male patient diagnosed with COVID-19 was admitted to an Intensive Care Unit (ICU). During hospitalization, the patient displayed episodes of bacteremia by <em>Staphylococcus haemolyticus</em> and a possible urinary tract infection by <em>T. asahii</em>. While the bacterial infection was successfully treated using broad-spectrum antibiotics, the fungal infection in the urinary tract was unsuccessfully treated with anidulafungin and persisted until the patient died.</p></div><div><h3>Conclusions</h3><p>With the evolving COVID-19 pandemic, invasive fungal infections have been increasingly reported, mainly after taking immunosuppressant drugs associated with long-term broad-spectrum antibiotic therapy. Although <em>Candida</em> and <em>Aspergillus</em> are still the most prevalent invasive fungi, <em>T. asahii</em> and other agents have emerged in critically ill patients. Therefore, a proper surveillance and diagnosing any fungal infection are paramount, particularly in COVID-19 immunocompromised populations.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 2","pages":"Pages 54-56"},"PeriodicalIF":1.9,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40471897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epizootic lymphangitis: A neglected disease of working equids","authors":"F. Javier Cabañes","doi":"10.1016/j.riam.2021.11.001","DOIUrl":"10.1016/j.riam.2021.11.001","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 1","pages":"Pages 4-5"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39769898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lodderomyces elongisporus: ¿agente causal de vaginitis?","authors":"Laura Sante ,&nbsp;Patricia Capón ,&nbsp;Amparo Coira Nieto ,&nbsp;Pilar Alonso-García","doi":"10.1016/j.riam.2021.11.002","DOIUrl":"10.1016/j.riam.2021.11.002","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 1","pages":"Page 28"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39871023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ángela Restrepo Moreno: The wise woman par excellence!","authors":"","doi":"10.1016/j.riam.2022.03.001","DOIUrl":"10.1016/j.riam.2022.03.001","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 1","pages":"Page 1"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1130140622000146/pdfft?md5=459009a894a664af30b0647cf405382d&pid=1-s2.0-S1130140622000146-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133554411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}