Revista Iberoamericana De Micologia最新文献

{"title":"Isolation of Cryptococcus gattii (genotype VGI) from a specimen of Pinus. An approach to its natural habitat in México City","authors":"Laura Rosio Castañón Olivares,&nbsp;Yurixhi Paola Aguilera León,&nbsp;Fernanda Marlene Moreno Manrique,&nbsp;José Enrique Reyes Lira,&nbsp;Elva Bazán Mora,&nbsp;Edith Sánchez Paredes","doi":"10.1016/j.riam.2025.09.002","DOIUrl":"10.1016/j.riam.2025.09.002","url":null,"abstract":"<div><h3>Background</h3><div>In Mexico, <em>Cryptococcus gattii sensu lato</em> has been identified as the etiologic agent in approximately 10.8% of cryptococcosis cases; however, its isolation from natural sources, which would confirm its existence in the environment, has not been successful.</div></div><div><h3>Objective</h3><div>To isolate <em>C. gattii</em> from environmental samples collected from trees within the movement area of a patient diagnosed with cryptococcosis caused by this species.</div></div><div><h3>Methods</h3><div>Based on a database of clinical isolates characterized as <em>C. gattii</em>, a patient was contacted and his route of movement was obtained; through a geospatial analysis, nearby trees were located. Tree hollows were sampled and the <em>Cryptococcus</em> isolates obtained were biochemically and genetically typed.</div></div><div><h3>Results</h3><div>Four <em>Cryptococcus</em> isolates were obtained from the trees <em>Schinus molle</em>, <em>Erythrina coralloides</em> and from a specimen of the genus <em>Pinus</em>: three of them were characterized as <em>Cryptococcus neoformans sensu stricto</em> and one as <em>C. gattii sensu stricto</em>.</div></div><div><h3>Conclusions</h3><div>Using a geographic information system led to delimit an environmental sampling area, resulting in the first documented report in Mexico of the isolation of <em>C. gattii</em> genotype VGI in nature.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"43 1","pages":"Pages 1-6"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145638235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infective endocarditis of a prosthetic mechanical valve caused by Trichosporon inkin in an immunocompetent patient: Case report","authors":"Maria Jose Amaya ,&nbsp;Ana Maria del Pilar Monroy ,&nbsp;Natalia Perez ,&nbsp;Paula Daniela Villamizar ,&nbsp;Juan Pablo Cardenas ,&nbsp;Diego Alejandro Pinto Pinzón","doi":"10.1016/j.riam.2025.10.002","DOIUrl":"10.1016/j.riam.2025.10.002","url":null,"abstract":"<div><h3>Background</h3><div>Infective endocarditis caused by <em>Trichosporon inkin</em> is an exceptionally rare fungal infection, particularly in immunocompetent patients. This microorganism is part of the normal microbiota of the skin and gastrointestinal tract, but can cause invasive infections associated with prosthetic devices or complex clinical conditions. Reported cases in the literature are scarce, making it difficult to establish standardized diagnostic and therapeutic guidelines.</div></div><div><h3>Case report</h3><div>We report the case of an immunocompetent female patient with rheumatic fever due to which she underwent a mitral valve replacement procedure using a mechanical prosthesis, who developed infective endocarditis caused by <em>T. inkin</em>. The initial diagnosis was delayed due to misinterpretation as contaminant of the fungal isolate recovered. The patient developed intracerebral hemorrhage and was considered high-risk for surgical intervention; thus, medical management was pursued. The microorganism was finally identified by MALDI-TOF mass spectrometry, a validated tool for identifying <em>Trichosporon</em> species. The patient received voriconazole, with favorable clinical response, followed by prolonged suppressive fluconazole therapy.</div></div><div><h3>Conclusions</h3><div>This is the second case reported in the Americas and the fifth worldwide of <em>T. inkin</em> infective endocarditis in an immunocompetent patient. It highlights the importance of considering this pathogen in prosthetic valve endocarditis in cases of unusual culture results, and demonstrates the diagnostic value of MALDI-TOF. Long-term antifungal therapy with triazoles, particularly voriconazole followed by fluconazole, may be an effective alternative for patients who are not surgical candidates.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"43 1","pages":"Pages 23-26"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145782714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the combined impact of amphotericin B and posaconazole on the germination of a rare Rhizopus homothallicus isolate responsible for COVID-19 associated mucormycosis","authors":"Aishwarya Nikhil ,&nbsp;Atul Kumar Tiwari ,&nbsp;Suchita Gautam ,&nbsp;Ragini Tilak ,&nbsp;Mohit Bhatia ,&nbsp;Munesh Kumar Gupta ,&nbsp;Roger J. Narayan","doi":"10.1016/j.riam.2025.10.003","DOIUrl":"10.1016/j.riam.2025.10.003","url":null,"abstract":"<div><h3>Background</h3><div>During the COVID-19 pandemic, <em>Rhizopus arrhizus</em> and <em>Rhizopus homothallicus</em> were identified as causative agents of rhino-cerebral mucormycosis in COVID-19 patients. Clinical management typically includes surgical debridement and antifungal treatment, with amphotericin B (AMB) and posaconazole (PCZ) as primary options. However, long-term use of AMB can lead to toxicity, necessitating PCZ as a follow-up treatment.</div></div><div><h3>Aims</h3><div>This study aimed to examine the combined effect of AMB and PCZ on <em>R. homothallicus</em>.</div></div><div><h3>Methods</h3><div>The combined effect of AmB and PCZ on <em>R. homothallicus</em> was studied by examining their minimum inhibitory concentration (MIC) values, fractional inhibitory concentration index (FICI), spore germination properties, viability, and intracellular reactive oxygen species accumulation; Raman spectroscopy was also performed.</div></div><div><h3>Results</h3><div>The MIC values for AmB and PCZ were 4 and 8<!--> <!-->μg/mL, respectively, with a combined MIC of 2<!--> <!-->μg/mL and an FICI index of &lt;0.28. The germination rates at MIC values after 48<!--> <!-->h of exposure were 28% for AmB, 36% for PCZ, and only 3% for the combination. Viability assays revealed dead sporangiospores following combination treatment. AmB and its combination generated more ROS (68.18% and 64.45%, respectively) than did PCZ alone (42.6%).</div></div><div><h3>Conclusions</h3><div>Combination therapy reduced the AMB dose without loss of efficacy, suggesting a synergistic effect against <em>R. homothallicus</em>. These results may support this alternative strategy to mitigate the side effects of AMB.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"43 1","pages":"Pages 7-16"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145800765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of risk factors and prognosis of fluconazole-resistant Candida tropicalis bloodstream infection","authors":"Fei Wang ,&nbsp;Yake Yao ,&nbsp;Qing Yang ,&nbsp;XueMei Lin ,&nbsp;Yihua Zheng ,&nbsp;Hua Zhou ,&nbsp;Qin Huang","doi":"10.1016/j.riam.2025.11.001","DOIUrl":"10.1016/j.riam.2025.11.001","url":null,"abstract":"<div><h3>Background</h3><div>In China, the isolation rate of fluconazole-resistant isolates of <em>Candida tropicalis</em>, together with fatality in cases of bloodstream infections due to this yeast, have increased annually.</div></div><div><h3>Aims</h3><div>This study investigates the clinical characteristics, risk factors, and prognostic factors of fluconazole-resistant <em>C. tropicalis</em> bloodstream infections (BSI).</div></div><div><h3>Methods</h3><div>A retrospective study analyzed clinical data of patients with <em>C. tropicalis</em> BSI from July 2013 to June 2019, focusing on clinical characteristics, risk factors, treatment regimens, and prognosis. Univariate analysis of risk factors and prognosis was conducted using <em>χ</em>² test or Fisher's exact tests. Binary logistic regression model for risk factors, and Cox regression method for prognosis, were used for multivariate analysis.</div></div><div><h3>Results</h3><div>The study enrolled 100 patients with <em>C. tropicalis</em> BSI, including 44 fluconazole-resistant and 56 fluconazole-sensitive cases; 64 patients were cured and 36 died, resulting in a mortality rate of 36%. Logistic regression analysis identified exposure to azole antifungal agents during the 2 weeks prior to the onset of the BSI as a risk factor for fluconazole resistance. Cox regression analysis showed that hematological malignancy, fluconazole-resistant strains, indwelling catheters, and chronic obstructive pulmonary disease were independent risk factors for patient mortality. Conversely, targeted therapy with sensitive antifungal agents and removal of drainage tubes were protective factors for survival.</div></div><div><h3>Conclusions</h3><div>Azole exposure led to the development of fluconazole resistance in <em>C. tropicalis</em> BSI; hematologic malignancies, azole resistance, chronic obstructive pulmonary disease and having intravenous catheters increased mortality rate. The use of echinocandins or amphotericin B and catheter removal improved outcomes, underscoring the need for early resistance detection and targeted treatment.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"43 1","pages":"Pages 17-22"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic endemic mycoses: From a geographical risk to a concern expansion","authors":"Gustavo Giusiano","doi":"10.1016/j.riam.2025.07.001","DOIUrl":"10.1016/j.riam.2025.07.001","url":null,"abstract":"<div><div>Blastomycosis, coccidioidomycosis, histoplasmosis, paracoccidioidomycosis, emergomycosis, and talaromycosis are sapronoses restricted to specific geographical areas associated with particular environmental niches that can lead to high morbidity and mortality if diagnosed or treated late. Challenges in clinical awareness arise from their nonspecific clinical presentations and the limited availability of sensitive diagnostic tools. Although advances in molecular diagnosis and antigen detection-based tests are promising, they are not available for all systemic endemic mycoses (SEM). Treatment guidelines emphasize the importance of early antifungal treatment, although only a few limited therapeutic options have remained available over the years. Most SEM are not notifiable diseases, resulting in underreporting and a poor understanding of their true burden. The inclusion of several SEM-causing dimorphic fungi in the WHO Fungal Priority Pathogens List highlights their growing public health impact, the lack of prevention methods, and unmet research needs. Recognizing SEM as neglected diseases, improving surveillance, expanding diagnostic access, and developing affordable vaccines and therapeutics are essential steps toward addressing this evolving global health challenge. Coordinated international efforts are urgently needed.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"42 3","pages":"Pages 87-92"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Naganishia diffluens (formerly Cryptococcus diffluens) from skin lesions of a patient with leukemia","authors":"Silvana Ramadán ,&nbsp;Lucia Bulacio ,&nbsp;Hernán Dalmaso ,&nbsp;Adriana Rocaspana ,&nbsp;Alicia Quarta ,&nbsp;Rita David ,&nbsp;Cecilia Capitelli ,&nbsp;Paula Funes ,&nbsp;María Mercedes Romero ,&nbsp;Constanza Taverna ,&nbsp;Susana Córdoba ,&nbsp;Maximiliano Sortino","doi":"10.1016/j.riam.2025.10.001","DOIUrl":"10.1016/j.riam.2025.10.001","url":null,"abstract":"<div><h3>Background</h3><div>Fungal diseases caused by species of the genus <em>Cryptococcus</em> are a growing public health problem, with the species belonging to the <em>Cryptococcus neoformans</em> and <em>Cryptococcus gattii</em> complexes being the most common and studied. In recent years, other species belonging to related genera have emerged, including <em>Naganishia diffluens</em>. As there are few reports on this species, it is important to emphasize its significance, particularly given that it has been found in skin lesions in immunocompromised patients, as in the case presented here. This highlights the importance of paying due attention to this species and recognizing its clinical relevance.</div></div><div><h3>Case report</h3><div>A 27-year-old male patient with a diagnosis of acute promyelocytic leukemia M3 (APL M3) experienced a febrile episode with skin lesions compatible with fungal infection, coinciding with marked neutropenia and the start of chemotherapy. Mycological analysis of skin lesions allowed the isolation of a fungus that was identified as <em>N. diffluens</em>.</div></div><div><h3>Conclusions</h3><div>The increase in the frequency of <em>N. diffluens</em> infections can be attributed to the rising number of patients with risk factors. The use of new microbial identification tools, such as molecular biology, which made it possible to accurately identify the causative agent, is highlighted.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"42 3","pages":"Pages 103-106"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145565036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shifting epidemiology and microbiological profiles of cryptococcosis in a Brazilian tertiary center: A descriptive study of 94 patients","authors":"Suzana Mesquita Ribeiro ,&nbsp;Vítor Falcão de Oliveira ,&nbsp;Ana Paula Cury ,&nbsp;Hélio Rodrigues Gomes ,&nbsp;Mariane Taborda ,&nbsp;Adriana Satie Gonçalves Kono Magri ,&nbsp;Guilherme Diogo Silva ,&nbsp;Afonso Rafael da Silva Junior ,&nbsp;Adriana Lopes Motta ,&nbsp;Wdson Luis Lima Kruschewsky ,&nbsp;Evangelina da Motta Pacheco Alves de Araujo ,&nbsp;Marcello Mihailenko Chaves Magri","doi":"10.1016/j.riam.2025.07.002","DOIUrl":"10.1016/j.riam.2025.07.002","url":null,"abstract":"<div><h3>Background</h3><div>The epidemiology of cryptococcosis is shifting, with an increasing number of cases occurring among non-HIV immunosuppressed individuals. In this context of a new epidemiological presentation of the disease, further studies are needed to better describe the microbiological profiles associated with different immune status.</div></div><div><h3>Aims</h3><div>To characterize cryptococcosis cases according to immune status, focusing on species distribution and time to culture positivity.</div></div><div><h3>Methods</h3><div>We conducted a retrospective study of patients with positive <em>Cryptococcus</em> spp. cultures from various biological materials between 2017 and 2022 at a Brazilian tertiary hospital. Clinical and laboratory data were extracted, including immune status, biological sample type, <em>Cryptococcus</em> species, and time to positivity in aerobic and fungal cultures. Patients were classified into HIV-positive, non-HIV immunosuppressed, or non-immunosuppressed/non-HIV. Descriptive analyses were performed.</div></div><div><h3>Results</h3><div>Among the 94 patients analyzed, 29 (30.9%) were HIV-positive, 39 (41.5%) were non-HIV immunosuppressed, and 19 (20.2%) were non-immunosuppressed/non-HIV. A total of 288 isolates were identified, predominantly <em>Cryptococcus neoformans</em> (72/94, 76.6%), but <em>Cryptococcus gattii</em> (17/94, 18%) infection was non-neglectable. The mean time to culture positivity was 5 days (range 2–24 days) for aerobic cultures and 10 days (range 0–63 days) for fungal cultures.</div></div><div><h3>Conclusions</h3><div>This work reinforces the need for future studies to better represent non-HIV immunosuppressed patients, to address infections caused by <em>C. gattii</em>, and to consider the wide variability in culture growth time as a diagnostic limitation.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"42 3","pages":"Pages 93-97"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of dermatophytes isolated from superficial dermatological samples taken during 2020–2023 in Zaragoza (Spain)","authors":"Eva Pablo-Hernando ,&nbsp;Javier Alcón ,&nbsp;María Riesgo ,&nbsp;Rafael Benito","doi":"10.1016/j.riam.2025.09.001","DOIUrl":"10.1016/j.riam.2025.09.001","url":null,"abstract":"<div><h3>Background</h3><div>Dermatophytoses are transmissible infections that affect one billion people worldwide and have a significant impact on public health. Dermatophytes distribution evolves geographically and over time. Consequently, local epidemiology should be periodically assessed to control infection.</div></div><div><h3>Aims</h3><div>To describe the local distribution of dermatophytes and types of dermatophytosis through the analysis of samples from patients with suspected superficial mycoses, and to identify areas of improvement.</div></div><div><h3>Methods</h3><div>A retrospective epidemiological analysis of mycological culture results from skin, hair and nail samples referred to the mycology lab in the Hospital Clínico Universitario Lozano Blesa, Zaragoza (Spain) between 2020 and 2023 was performed, and the results statistically analyzed.</div></div><div><h3>Results</h3><div>4371 specimens (skin: 41.4%; hair: 5.1%; nails: 53.5%) were cultured using standard procedures. The demand for testing increased by 53% over a 4-year time period and a dermatophyte positivity rate of 16.7% (<em>n</em> <!-->=<!--> <!-->731) was found. The species distribution was the following: <em>Trichophyton rubrum</em> (56%), <em>Trichophyton tonsurans</em> (11.3%), <em>Microsporum canis</em> (11.8%), <em>Trichophyton interdigitale</em> (7%), <em>Trichophyton. mentagrophytes</em> (5.8%), <em>Microsporum audouinii</em> (3.8%) and other species (4.9%), with an anthropophilic to zoophilic ratio of 4:1. The dermatophytoses clinical forms found were <em>tinea unguium</em> (39.6%), associated to population over 45 years, <em>tinea corporis</em> (24.7%) and <em>tinea capitis</em> (10.6%), both associated to people with less than 16 years group, <em>tinea pedis</em> (17.5%), mainly observed in people aged 31–45 years; other forms accounted for 7.6%. Finally, the Emergency department requested 11.9% of the mycological tests.</div></div><div><h3>Conclusions</h3><div>Local epidemiology of dermatophytoses highlights the predominance of anthropophilic species, whereas 35 years ago zoophilic species represented 80% of the isolates. Interestingly, mild superficial lesions are frequently and inappropriately brought to the Emergency department.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"42 3","pages":"Pages 98-102"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145431741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical insights into invasive aspergillosis among immunosuppressed patients: A single-centre experience from Argentina","authors":"Fernando Riera ,&nbsp;Julieta Carballo ,&nbsp;Carlos Bergallo ,&nbsp;Federico Romero ,&nbsp;Belén Palacio ,&nbsp;Lizet Luque-Aguada ,&nbsp;Marcos Marino ,&nbsp;Jon Salmanton-García","doi":"10.1016/j.riam.2025.01.003","DOIUrl":"10.1016/j.riam.2025.01.003","url":null,"abstract":"<div><h3>Background</h3><div>Invasive aspergillosis poses a significant threat to immunocompromised individuals. Diagnostic criteria incorporating biomarkers and imaging have improved diagnosis, and treatment options have expanded. However, in Argentina, diverse patient demographics and environmental factors add complexity to managing this infection.</div></div><div><h3>Aims</h3><div>This study aims to explore the epidemiology, diagnostic methods, and treatment of invasive aspergillosis in an Argentine hospital setting.</div></div><div><h3>Methods</h3><div>We collected data from patients with suspected invasive aspergillosis at a tertiary care hospital in Central-Northern Argentina. Variables included demographics, underlying conditions, diagnostic criteria, treatment, and outcomes.</div></div><div><h3>Results</h3><div>With a median age of 44.5 years and a 51% of male patients, our institution conducted invasive aspergillosis screenings on 192 patients, many of whom were battling malignancies (90%). One third of them had the infection set as probable or possible. Imaging (31%) and positive microbiological results (16%) were examples of diagnostic evidence. With an overall mortality rate of 15%, half of the patients got antifungal treatment for a median of seven days. Mortality among the diagnosed patients was 22%. Patients without stem-cell transplantation had a high death rate (31%), although this difference was not statistically significant; in patients having pulmonary nodules (15%) the death rate was not statistically significant either. There were no discernible variations in mortality according to the type of treatment received.</div></div><div><h3>Conclusions</h3><div>Our study reveals that invasive aspergillosis remains a significant issue in high-risk patients, and has a notable mortality rate, particularly among those patients with pulmonary nodules. Computed tomography provides a high diagnostic yield.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"42 2","pages":"Pages 55-59"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluconazole in combination with compounds of natural and pharmaceutical origin: A study on their antimicrobial activity against Candida auris","authors":"Iñigo de-la-Fuente ,&nbsp;Andrea Guridi ,&nbsp;Guillermo Quindós ,&nbsp;Elena Eraso ,&nbsp;Elena Sevillano","doi":"10.1016/j.riam.2025.05.001","DOIUrl":"10.1016/j.riam.2025.05.001","url":null,"abstract":"<div><h3>Background</h3><div>Candidiasis is a prevalent and clinically significant fungal infection. <em>Candida auris</em> has emerged as an important etiological agent due to its high transmissibility, potential to cause severe infections, and resistance to multiple antifungal drugs. Combining fluconazole with other compounds to achieve additive or synergistic antifungal effects has been proposed as a promising strategy for managing these infections.</div></div><div><h3>Aims</h3><div>This study aimed to evaluate the antifungal effects of fluconazole in combination with seven natural compounds (berberine chloride, caffeic acid phenethyl ester, catechin monohydrate, epigallocatechin gallate, magnolol, osthole, and quercetin hydrate) and seven drugs (amiodarone, amlodipine, budesonide, doxycycline, ibuprofen, minocycline, and tigecycline) against <em>C. auris</em>.</div></div><div><h3>Methods</h3><div>The antifungal activity of fluconazole alone and in combination with selected compounds and drugs was analyzed to identify additive or synergistic interactions against 21 isolates of <em>C. auris</em>. Minimum inhibitory concentrations (MICs) of fluconazole were determined and compared across combinations.</div></div><div><h3>Results</h3><div>Fluconazole combined with magnolol, caffeic acid phenethyl ester, or quercetin hydrate showed additive effects against 38%, 9.5%, and 2% of isolates, respectively, and a synergistic effect with quercetin hydrate in 19% of isolates. Fluconazole MICs were reduced from &gt;64<!--> <!-->μg/mL to 1–16<!--> <!-->μg/mL with magnolol in 19 isolates, to 1–32<!--> <!-->μg/mL with caffeic acid phenethyl ester in 17 isolates, and to 1–2<!--> <!-->μg/mL with quercetin hydrate in 8 isolates.</div></div><div><h3>Conclusions</h3><div>These findings highlight the potential of fluconazole combined with magnolol, caffeic acid phenethyl ester, or quercetin hydrate for managing <em>C. auris</em> infections. Further in vivo studies are warranted to assess their therapeutic applicability.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"42 2","pages":"Pages 74-80"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}