Research Journal of Pharmacy and Technology最新文献_第3页

Bioequivalence Study and Bioanalytical Method Development of Remogliflozin etabonate tablets in wistar rat plasma using RP-HPLC Method 采用 RP-HPLC 方法对瑞莫利嗪依他朋酯片剂在雾大鼠血浆中的生物等效性进行研究并开发生物分析方法

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00122

N. Tamilselvi, K. Kanagapriya

{"title":"Bioequivalence Study and Bioanalytical Method Development of Remogliflozin etabonate tablets in wistar rat plasma using RP-HPLC Method","authors":"N. Tamilselvi, K. Kanagapriya","doi":"10.52711/0974-360x.2024.00122","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00122","url":null,"abstract":"A new RP-HPLC method for the bioequivalence study of two RGE (Remogliflozin etabonate) formulations in wistar rat plasma has been developed using Rosuvastatin as internal standard and validated as per ICH guidelines. The plasma samples were extracted with methanol and chromatographic separation was achieved on phenomenex C18 (250 x 4.6mm, 5μ) analytical column with a mobile phase of methanol: 0.2% TEA (pH adjusted to 3 with orthophosphoric acid) in the ratio of 78:22% v/v and detection at 227nm. Calibration curve was linear in the range of 100-600ng/ml and the r2 value was found to be 0.9906. The extraction efficiency of RGE from rat plasma at the concentration of 50, 100 and 150% was found to be 99.6%, 99.9% and 98.05%. The Intra-day precision and interday precision was found to be 0.14 - 0.777% and 0.0070% - 0.0572% respectively. The maximum concentration (Cmax) obtained for two brands ( selected brand 1 & 2) formulation are 57.04 and 51.99 ng/ml respectively. The half life (t1/2) of two brands are calculated as 1 h. Area under the curve AUC 0- t of brand 1 and brand 2 is calculated as 343.235 and 310.1775ng h/ml and AUC 0-∞ is calculated to be 465.3665 and 501.4235ng h/ml respectively. The present study shows no significant difference in pharmacokinetic parameters between two products so the two formulations are considered to be bioequivalent.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"253 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacognostic, Physicochemical, Phytochemical and Chromatographic Characterization of Vaividanga Choornam, A Siddha Polyherbal Formulation 一种 Siddha 多草药配方 Vaividanga Choornam 的药理、理化、植物化学和色谱表征

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00113

P. Parvathy, M. Natarajan, B. Kannan, G. Lekha, A. Kanagarajan

{"title":"Pharmacognostic, Physicochemical, Phytochemical and Chromatographic Characterization of Vaividanga Choornam, A Siddha Polyherbal Formulation","authors":"P. Parvathy, M. Natarajan, B. Kannan, G. Lekha, A. Kanagarajan","doi":"10.52711/0974-360x.2024.00113","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00113","url":null,"abstract":"Standardization of herbal formulations as determined by the concentration of their activecomponents is essential to appraise the quality of drugs. The current study intends to investigate the phytochemical and physicochemical analyses of Vaividanga Choornam, a traditional Siddha polyherbal composition. This Vaividanga choornam contains the ingredients like Vaividangam (Embelica ribesBurm.), Milagu (Piper nigrumL.), Chukku (Zingiber officinale Rosc.), Omam (Trachyspermum ammi (L) Sprague), and Nilavaagai leaves (Cassia angustifolia Vahl. - syn. Cassia senna). It treatspitha diseases, constipation, indigestion, flatulence, burning sensation, bronchial asthma, and Kapha diseases. It is also an effective medicine for treating intestinal helminths caused by heavy worm burdens in young children. This study aims to estimate the quality of Vaividanga Choornam by performing pharmacognostic studies, preliminary phytochemical analysis, physiochemical parameters like ash value, extractive value, and loss on drying as per pharmacopoeial laboratory for Indian medicine guidelines, and also with the help of tests done with High-Performance Thin Layer Chromatography (HPTLC), FTIR Spectroscopy, and UV -Vis spectrometric analyses. The results obtained fromthose analyses showed the presence of various phytochemicals. The results indicate that the drug is of adequate quality and may be used to establish pharmacopeial standards of the medicine.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"39 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GC-MS Investigation of Unidentified Pharmaceutical ability of Indigenous herbaceous vine, Tinospora cordifolia’s fruits GC-MS 对本地草本藤本植物天竺葵果实中不明药用成分的研究

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00095

Akhilraj B. C., Suresh J., R. K., Kumar M., G. R

{"title":"GC-MS Investigation of Unidentified Pharmaceutical ability of Indigenous herbaceous vine, Tinospora cordifolia’s fruits","authors":"Akhilraj B. C., Suresh J., R. K., Kumar M., G. R","doi":"10.52711/0974-360x.2024.00095","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00095","url":null,"abstract":"As the majority of pharmaceutical businesses rely on medical plants for the synthesis of pharmaceutical chemicals, medicinal plants are of significant interest to researchers in the field of biotechnology. The majority of herbal remedies and the products that are derived from them are frequently made from unrefined plant extracts, which include a complex mixture of various phytochemical components (plant secondary metabolites). The chemical characteristics of these components vary greatly between species. An intriguing device for determining the concentration of various active constituents in plants utilised in the cosmetic, medicine, the agriculture or medical sectors is the GC-MS method employed for the assessment of the extracted samples. The entire plant methanol extract of Tinospora was used in this work to identify bioactive elements employing gas chromatography and mass spectrometry (GC-MS). The GC-MS results suggest several different chemicals, including (9E,11E)-Octadecadienoic acid , 9-Octadecenoic acid, (E)-, 9-Octadecenoic acid, Octadecanoic acid, .gamma.-Sitosterol, n-Hexadecanoic acid, 9-Octadecenoic acid (Z)-, 2,3-dihydroxypropyl ester, Methyl cis-4,7,10,13,16,19-Docosahexaenoate and 2-Hydrazino-2-imidazoline in the Tinospora methanolic extract. As a result, the bioactive compounds in the methanol extracts of this herb may have chemopreventive, antitumor, anti-microbial, antioxidants, and hypoglycaemic potential. This study's findings suggest that the existence of these phytochemicals as well as bioactive substances in Tinospora cordifolia makes it a potential new reservoir of medications.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"294 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140447301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overview on Natural Polymers: A Promising Pharmaceutical excipient in Mucoadhesive Drug Delivery System 天然聚合物概述：黏附性给药系统中一种前景广阔的药用辅料

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00142

Md Tamijur Rahman, Uzma Hismat, Ranjan Tripura, Paromita Dutta Choudhury, Bikash Debnath, Suvakanta Das

{"title":"Overview on Natural Polymers: A Promising Pharmaceutical excipient in Mucoadhesive Drug Delivery System","authors":"Md Tamijur Rahman, Uzma Hismat, Ranjan Tripura, Paromita Dutta Choudhury, Bikash Debnath, Suvakanta Das","doi":"10.52711/0974-360x.2024.00142","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00142","url":null,"abstract":"This review aims to compile the current literature with a perspective on the role of natural polymers or polysaccharides in mucoadhesive drug delivery systems. Natural polymers are basically polysaccharides, biocompatible, non-toxic, stable, and economical. Polymers play an important role as excipients in designing the dosage form and influence drug release without any side effects. Natural polymers are of growing interest in the utilization of mucosal drug delivery as a novel technique due to their ability to interact with the mucosal layer of the body, improve the effectiveness of the drug, and prolong the residence time of the dosage form at the site of absorption. The bioadhesive polymers can exert control over the rate and extent of drug release and are selected in the formulation of mucoadhesive drug delivery systems. This review article discusses several aspects of mucoadhesive drug delivery, including the mechanism of mucoadhesion, mucus layer, and mucoadhesive polymers, with special reference to natural-based polysaccharides, as well as their applications and roles in different formulations developed by using polysaccharides in various dosage forms.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"37 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140449266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation the Anti-leukemia effect of Methanol extracts of Camellia cuongiana on the K562 cell line 评估山茶甲醇提取物对 K562 细胞株的抗白血病作用

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00088

Pham Hoai Linh, Bui Thi Kim Ly, Hoang Thanh Chi

引用次数: 0

Identification of compounds from Zingiber officinale as Novel Inhibitor for Dengue DEN2 NS2B/NS3 Serine Protease through Molecular Docking and DFT approaches 通过分子对接和 DFT 方法鉴定作为登革热 DEN2 NS2B/NS3 丝氨酸蛋白酶新型抑制剂的姜科植物中的化合物

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00123

N. Frimayanti, Marzieh Yaeghoobi, Seyed Jamal Ashrafi, Bibi Fatemeh Haghirosadat, Melzi Octaviani, Annisya Rahmi

{"title":"Identification of compounds from Zingiber officinale as Novel Inhibitor for Dengue DEN2 NS2B/NS3 Serine Protease through Molecular Docking and DFT approaches","authors":"N. Frimayanti, Marzieh Yaeghoobi, Seyed Jamal Ashrafi, Bibi Fatemeh Haghirosadat, Melzi Octaviani, Annisya Rahmi","doi":"10.52711/0974-360x.2024.00123","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00123","url":null,"abstract":"Dengue virus (DENV) is one type of virus belongs to the Flavivirus family that can be transmitted through mosquito bites. Infection with the dengue virus can cause different febrile symptoms, such as dengue fever (DD) and dengue haemorrhagic fever (DHF), with or without shock. The purpose of this study is to obtain a new compound from Zingiber officinale that is expected to have potential bioactivity against DENV-2 NS2B/NS3 serine protease. A computational approach was applied in this study; which began with docking of compounds into protein targets, followed by density functional theory, drug-likeness, and ADMET analysis. According to the calculation, it was determined that compound 9 has binding interactions with the active triad through amino acids His51, Asp75, and Ser135. Additionally, drug-likeness and ADMET analysis for compound 9 showed that it has optimal lipophilicity and, when administered orally, can achieve good bioavailability. It is indicated that compound 9 can be used as a promising and potential inhibitor for DENV-2 NS2B/NS3 serine protease.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"46 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140447686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of Procalcitonin with Acid Fast Bacilli and Gene Xpert MTB/RIF as a Marker of Treatment Progress in Pulmonary Tuberculosis patients 作为肺结核患者治疗进展标志物的降钙素原、酸性快速杆菌和基因 Xpert MTB/RIF 的相关性

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00103

Baiq Nasha Islaeli, Puspa Wardhani, A. Aryati, T. Kusmiati

{"title":"Correlation of Procalcitonin with Acid Fast Bacilli and Gene Xpert MTB/RIF as a Marker of Treatment Progress in Pulmonary Tuberculosis patients","authors":"Baiq Nasha Islaeli, Puspa Wardhani, A. Aryati, T. Kusmiati","doi":"10.52711/0974-360x.2024.00103","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00103","url":null,"abstract":"There are several limitations in using AFB and GeneXpert to evaluate the treatment of TB patients, one of which is influenced by sputum quality. Therefore, an alternative method is needed to help evaluate the treatment of TB patients. This study aimed to analyze the correlation of the Procalcitonin test with AFB and GeneXpert for evaluating the treatment of TB patients and the performance of Procalcitonin as a marker of TB patient treatment progress. A prospective cohort study was conducted from May to September 2022 at the West Nusa Tenggara General Hospital, Indonesia. Sputum and blood samples were collected from 36 patients who were confirmed positive for TB by GeneXpert MTB/RIF examination, then examined for procalcitonin and AFB before being given treatment and after the intensive phase of treatment. Procalcitonin tested with VIDAS Biomerieux and VIDAS BRAHMS PCT kit. Procalcitonin did not correlate with AFB (p=0.064, r= 0.327) and GeneXpert before treatment (p=0.169, r=0.245), but correlated with AFB (p=0.013, r=0.427) and GeneXpert MTB/RIF (p=0.020, r=0.405) after the intensive phase of treatment. Procalcitonin test with a cut-off value of 0.07 detected negative AFB cases after treatment with a sensitivity of 28.6 and a specificity of 96.2%. The procalcitonin cut-off value of 0.07 also detected negative Xpert MTB/RIF after treatment with a sensitivity of 16.7% and a specificity of 100%. The performance of Procalcitonin for detecting negative smear and negative Xpert MTB/RIF after the intensive phase of treatment is classified as having high specificity, but its sensitivity is still low. Future studies are needed to evaluate the performance of Procalcitonin compared to bacterial cultures.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140447697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycerine addition in Cashew stembark extract mouthwash influence the inhibition of bacterial growth 腰果提取物漱口水中添加甘油对抑制细菌生长的影响

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00138

Harsini Harsini, D. Irnawati, Dyah Anindya Widyasrini, S. Sunarintyas, W. Siswomihardjo

{"title":"Glycerine addition in Cashew stembark extract mouthwash influence the inhibition of bacterial growth","authors":"Harsini Harsini, D. Irnawati, Dyah Anindya Widyasrini, S. Sunarintyas, W. Siswomihardjo","doi":"10.52711/0974-360x.2024.00138","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00138","url":null,"abstract":"The part of cashew plant (Anacardium Occidentale Linn) can be utilized as an ingredient in herbal medicine. Alkaloids, tannins, and flavonoids found in cashew stembark exhibit broad-spectrum antibacterial activity, making them appropriate as an active ingredient in mouthwash. On the other hand, one of the key ingredients in mouthwash formulas is glycerin. It is also frequently used as humectant. This study aims to prove the effect of glycerin on the cashew stembark extract mouthwash on its antibacterial activity. This study used 4 formulas consisted of 7% concentration of cashew stembark extract added with 1% sorbitol, 1.5% peppermint, distilled water as solvent, and various concentration of glycerin for each formula. The clarity, color change, and taste were assessed at day 7. The growth inhibition of both gram positive (S. aureus) and gram negative (P. aeruginosa) bacteria was measured. The data obtained were tested using one-way ANOVA and LSD (α=0.05). All of the 4 formulas of the mouthwashes found to be relatively stable. Based on the results of the inhibition test, it can be concluded that the addition of glycerin affects the inhibition of S. aureus and P. aeruginosa bacteria. Glycerin had significant effect on bacterial growth inhibition at 15% (Formula III).","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"388 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140447912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antihypertensive effect of Dapagliflozin in Type II diabetic patients with Hypertension 达帕格列净对 II 型糖尿病高血压患者的降压作用

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00128

Samah Hammoud, Arige Boubou, Nisrin Kaddar

{"title":"Antihypertensive effect of Dapagliflozin in Type II diabetic patients with Hypertension","authors":"Samah Hammoud, Arige Boubou, Nisrin Kaddar","doi":"10.52711/0974-360x.2024.00128","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00128","url":null,"abstract":"Hypertension (HTN) and type II diabetes mellitus (T2DM) are two main cardiovascular risk factors that coexist frequently. The aim of this study is evaluation the antihypertensive effect of dapagliflozin in hypertensive type 2 diabetic patients (controlled/inadequately uncontrolled), and study its effect on contributing factors in that effectiveness (ex. weight loss and SNS damping). Primary endpoints included the effect of dapagliflozin on hemoglobin A1C (HbA1c) and blood pressure. Secondary endpoints included the effect of drug on weight body, pulse rate and pulse pressure. Patients were randomly selected from the outpatient of the Endocrinology Department at Tishreen University Hospital in Syria, and divided into two groups: dapagliflozin group (n=45) received 5 or 10mg dapagliflozin once daily as an add-on therapy to oral anti-diabetic drugs (OAD)and antihypertensive drugs, and control group (n=38) received OAD and antihypertensive drugs. After 12 weeks follow-up, dapagliflozin-treated versus control-treated showed significant reduction in HbA1C (-0.7% vs -0.3%, p<0.05, mean seated SBP (-9.4 vs -1.2mmHg, p=0.0001) and mean seated DBP(-6 vs-0.3mmHg, p=0.0001). Dapagliflozin also reduced body weight compared with control (-2.6 vs -0.6kg, p=0.0001) andthere was a significant positive correlation between weight loss and BP reduction. The effect on pulse rate was modest and similar between two groups but dapagliflozin have lowered pulse pressure values. There was no significant dose-response with any studied parameter, too. 5mg dapagliflozin once daily improved glycemic control, reduced body weight, SBP more than DBP and thus improved pulse pressure. All that without any significant effect on pulse rate.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"84 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficiency Evaluation and Characterization of Microbially, Phyto and Chemically Synthesized Silver Nanoparticles 微生物、植物和化学合成银纳米粒子的效率评估和特性分析

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI: 10.52711/0974-360x.2024.00111

Abdelgyed M. Lela, Moataz H. El-Sehrawy, Mohamed A. Abdel-Rahman, A. El-Waseif

{"title":"Efficiency Evaluation and Characterization of Microbially, Phyto and Chemically Synthesized Silver Nanoparticles","authors":"Abdelgyed M. Lela, Moataz H. El-Sehrawy, Mohamed A. Abdel-Rahman, A. El-Waseif","doi":"10.52711/0974-360x.2024.00111","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00111","url":null,"abstract":"The present research objectives involves the synthesis and characterization of microbially, Phyto- and chemically silver nanoparticles (AgNPs) and evaluation the antimicrobial and cytotoxicity effect. Silver nanoparticles were biosynthesized through Streptomyces clavuligerus and Curcuma extract in addition to chemical reduction method with trisodium citrate as reducing agent silver nitrate as the metal substrate. The syntheses of the silver nanoparticles were characterized using UV-Vis spectroscopy and scanning electron microscopy (SEM). The results of characterization revealed absorption peaks obtained at range from 217-222 nm for all AgNPs types. SEM photographs of biosynthesized AgNPs indicate a narrow size distribution with average 8 nm in ‎spherical with smooth surfaces‎ and serruond protein but, chemical AgNPs showed ‎ without serruond protein. Antibacterial activity of AgNPs was determined using agar well diffusion method against Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginos. Results show similar significant antibacterial activity of microbial and Phyto- biosynthesized silver NPs. On the other hand, the chemically synthesized AgNPs recorded no activity against all tested pathogens. The cytotoxicity of AgNPs was tested in vitro on lung tissue fibroblast (WI-38) using MTT with various concentrations. The results recorded IC50 4.6, 1.25 and 2.18 µgml-1 for microbially, Phyto- and chemically AgNPs respectively. Almost all the microbial synthesis of silver nanoparticles exhibit the beast characteristics that qualify it for application in the pharmaceutical field due to its being the smallest in size, antimicrobial activity, and the less toxic on host cells.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"997 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140445983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0