Evaluation the Anti-leukemia effect of Methanol extracts of Camellia cuongiana on the K562 cell line

Abstract

This study aims to examine the anti-proliferation effects of Camellia cuongiana methanolic extracts on the K562 chronic myelogenous leukaemia cell line. The crude extract was obtained by soaking the sample with methanol, following concentration using a rotary evaporator. Trypan blue exclusion test was performed in five concentrations of extract to detect the anti-cancer characteristic of Camellia cuongiana. Morphological changes in the treatment cell were observed using a phase-contrast microscope. After 72 hours of treatment, the extracts inhibited cell proliferation, with IC50 values of 72.26±5.75µg/mL. Increasing the extract concentration decreased the number of living cells. The most frequently observed abnormal morphology was cell shrinkage. Evidently, Camellia cuongiana could inhibit cancer cell proliferation and induce programmed cell death.