Reproduction最新文献

{"title":"Nicotinamide mononucleotide supports the development of bovine embryo through enhancement of mitochondrial function in morula.","authors":"Shuting Ye, Shu Hashimoto, Masaya Yamanaka, Sanath Udayanga Kankanam Gamage, Yuki Inoue, Hisataka Iwata, Yoshiharu Morimoto, Daisuke Tachibana","doi":"10.1093/reprod/xaag128","DOIUrl":"https://doi.org/10.1093/reprod/xaag128","url":null,"abstract":"<p><p>NAD+ is an important regulator of cellular physiological processes. However, in the culture environment, NAD+ levels are predicted to decrease below physiological requirements. Therefore, we examined the effects of the NAD+ precursor nicotinamide mononucleotide (NMN) on NAD+ levels and viability of mammalian fertilized ova. NAD+ levels decreased during in vitro culture, but this decrease was alleviated by NMN. NMN also increased the blastocyst formation rate and accelerated the developmental rate. Transcriptome analysis showed that NMN induced significant changes in the expression of genes involved in mitochondrial function in morula, and that NMN increased mitochondrial membrane potential, oxygen consumption rate and mitochondrial DNA copy number of morula while reducing reactive oxygen species levels. The addition of NMN to the embryo culture medium increased NAD+ levels in embryos, boosted mitochondrial function at the morula stage, and enhanced their ability to develop into blastocysts.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Immune-Related Hub Genes as Candidate Biomarkers for Preeclampsia: Integrative Transcriptomic and Machine Learning Approaches.","authors":"Xianyang Hu, Lu Liu, Tingxuan Yin, Xixi Huang, Hailin Yu, Yang Yan, Tianyu Zhong, Meirong Du, Fuju Zhao","doi":"10.1093/reprod/xaag129","DOIUrl":"https://doi.org/10.1093/reprod/xaag129","url":null,"abstract":"<p><p>Preeclampsia (PE) is a major cause of maternal and perinatal morbidity and is increasingly recognized as a disorder involving immune dysregulation. However, reliable candidate biomarkers and key molecular alterations associated with PE remain unclear. We integrated multiple public placental transcriptomic datasets from patients with PE and normotensive controls. Weighted gene co-expression network analysis and eight machine-learning algorithms were used to identify stable immune-related candidate genes, followed by immune infiltration analysis, single-cell analysis, SHAP interpretation, and in silico gene knockout analysis. A six-gene logistic regression model was evaluated in the same merged placental dataset used for feature selection and explored in a small independent peripheral-blood cohort. Frequency-based integration across the eight algorithms identified a six-gene signature comprising ACSS3, F13A1, MCTP1, PTGFR, SLC16A10, and TNFRSF11A. The model showed apparent discrimination in the merged placental dataset (AUC = 0.869, 95% CI: 0.820-0.919) and exploratory cross-tissue discrimination in the peripheral-blood cohort (AUC = 0.860, 95% CI: 0.691-1.000). Immunohistochemical analysis of placental tissues from five women with PE and five normotensive controls showed lower protein abundance of all six candidates in PE, consistent with the bulk transcriptomic findings. Single-cell analysis indicated heterogeneous cell-type-dependent expression patterns, while in silico knockout analysis suggested potential macrophage-associated regulatory effects. Collectively, these exploratory findings identify a six-gene immune-related candidate signature associated with PE that requires validation in larger, prospectively collected cohorts before clinical application.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The contribution of circadian, sleep and hormonal rhythms to pregnancy outcomes: an opportunity for improved management of women during pregnancy.","authors":"Thu Van-Quynh Duong, Rene Cortese, Hanne M Hoffmann","doi":"10.1093/reprod/xaag127","DOIUrl":"https://doi.org/10.1093/reprod/xaag127","url":null,"abstract":"<p><p>Circadian rhythms are fundamental regulators of physiology and play a critical role in reproductive success. During pregnancy, precise temporal coordination of endocrine, metabolic, and immune processes is required to support maternal adaptation, placental function, and foetal development. Disruption of circadian rhythms through environmental factors such as light at night, shift work, or sleep disorders has emerged as a significant risk factor for adverse pregnancy outcomes, including preterm birth, gestational diabetes, and preeclampsia. In this review, we synthesize current evidence linking circadian and sleep disruption to pregnancy complications, with a particular focus on the temporal regulation of key hormones, including progesterone, oestrogen, cortisol, oxytocin, and melatonin. We discuss how altered rhythmicity of hormone production and tissue responsiveness may impair target tissue function, maternal cardiovascular adaptation, and the development of gestational diabetes. We highlight emerging mechanistic insights into molecular clock regulation across reproductive tissues and its interaction with environmental cues. Finally, we outline opportunities for clinical translation, including chronobiology-informed diagnostics and chronotherapeutic strategies, to improve pregnancy management and reduce the burden of adverse outcomes.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C/EBP Transcription Factors in Ovarian Biology: Orchestrating Follicle Development, Ovulation and Luteinization.","authors":"Kashish Jain, Hanxue Zhang, Holly A LaVoie, Yi A Ren","doi":"10.1093/reprod/xaag126","DOIUrl":"https://doi.org/10.1093/reprod/xaag126","url":null,"abstract":"<p><p>The mammalian ovary is a structurally and functionally dynamic tissue that undergoes cyclical changes involved in folliculogenesis, ovulation, and luteal function. Each process depends on the coordination of complex cell biology such as steroidogenesis, inflammation and vascular remodeling that are regulated at transcriptional and post-translational levels. CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors emerging as key regulators of these processes, in particular C/EBPα and C/EBPβ. The upstream regulation and downstream biological effects of C/EBPα and C/EBPβ are context-dependent and vary depending on the state of ovarian follicles and corpus luteum. These factors integrate local ovarian and endocrine signals to orchestrate ovarian function, and genetic disruption of their expression leads to obstructed primordial follicle formation, impaired ovulation, defective luteinization, and reduced fertility. This narrative review synthesizes current knowledge on the expression, regulation, and function of C/EBP transcription factors, particularly C/EBPα and C/EBPβ, in ovarian biology. It highlights the current knowledge gap and future directions in further defining the role of C/EBP transcription factors in ovarian function and dysfunction.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenic Properties of Human Endometriotic Epithelial Cells Require Steroid Receptor Coactivator-2.","authors":"Vineet K Maurya, Sivagami Gunasekaran, Nisha Misra, David M Lonard, Pooja Popli, Ramakrishna Kommagani, Samantha Hrbek, Yong Song, Niraj Joshi, Asgerally T Fazleabas, John P Lydon","doi":"10.1093/reprod/xaag125","DOIUrl":"https://doi.org/10.1093/reprod/xaag125","url":null,"abstract":"<p><p>Commonly diagnosed in reproductive-age women, endometriosis is causally linked to subfertility, infertility, and chronic pelvic pain. Although theories exist on the etiopathogenesis of endometriosis, our understanding of its cellular and molecular underpinnings remain incomplete. We report that steroid receptor coactivator-2 (SRC-2), a member of the p160/SRC family of transcriptional coregulators, is expressed predominantly in epithelial cells of ectopic endometriotic lesions in the primate and mouse. Using an immortalized human endometriosis epithelial cell (iHEEC) line in conjunction with siRNA-mediated silencing, we demonstrate that SRC-2 is essential for many of the pathogenic features ascribed to endometriotic lesion expansion, including sustained viability along with clonogenic, migratory, and invasive properties. Genome-scale iHEEC RNA-seq analysis implicates SRC-2 in a number of pathobiologies associated with the cellular phenotype of the ectopic endometriotic epithelial cell, including actin-cytoskeleton reorganization. To underscore the utility of this RNA-seq dataset, we show that S100A10, a cytoarchitectural scaffolding protein, is responsive to SRC-2 and essential for many of above cellular properties of the iHEEC that are ascribed to SRC-2. Because S100A10 dysregulation is strongly linked to the etiopathogenesis of a wide-spectrum of malignancies, many of the molecular mechanisms which underlie S100A10's role in cancer progression are predicted to be coopted in endometriosis. Therefore, these investigations highlight an unsuspected role for SRC-2 (and its transcriptome) in maintaining the pathogenetic phenotype of the ectopic endometriotic epithelial cell. Such findings, support continued investigations of this coactivator and its downstream gene expression programs as potential targets for the prognosis, diagnosis and/or treatment of this gynecological disorder in the future.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Thigmotactic navigation and functional enrichment of boar sperm in microfluidic channels.","authors":"","doi":"10.1093/reprod/xaag132","DOIUrl":"https://doi.org/10.1093/reprod/xaag132","url":null,"abstract":"","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":"172 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149288640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the clock: reprogramming fertility in diminished ovarian reserve.","authors":"Tingyu Lang, Xiaolei Liang, Yongxiu Yang","doi":"10.1093/reprod/xaag112","DOIUrl":"10.1093/reprod/xaag112","url":null,"abstract":"<p><p>In brief: Diminished ovarian reserve (DOR), a hallmark of female reproductive aging, impairs fertility and quality of life. Aging-reprogramming technologies offer new mechanistic and therapeutic insights. This review examines aging reprogramming in DOR-covering telomere shortening, mitochondrial dysfunction, and epigenetic alterations-alongside diagnostic criteria, biomarkers, and limitations of current treatments, highlighting emerging reprogramming-based interventions to improve reproductive health. Abstract: Diminished ovarian reserve (DOR) is a significant manifestation of female reproductive aging that profoundly impacts fertility and quality of life. In recent years, the emergence of aging-reprogramming technologies has provided new insights into the mechanisms underlying DOR and potential therapeutic approaches. This review synthesizes current knowledge on the role of aging reprogramming in DOR, focusing on key molecular events such as telomere shortening, mitochondrial dysfunction, and epigenetic alterations. Furthermore, we summarize clinical diagnostic criteria and emerging biomarkers for DOR, while exploring the limitations of existing treatment strategies and the potential interventions based on aging reprogramming. By addressing these issues, this review aims to highlight the interplay between aging processes and ovarian function, paving the way for innovative therapeutic avenues to improve reproductive health in women experiencing DOR.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian fluid is associated with distinct sperm RNA profiles and enhanced swimming performance in a teleost fish.","authors":"Alexandra E Nowicki, Ian A E Butts","doi":"10.1093/reprod/xaag113","DOIUrl":"10.1093/reprod/xaag113","url":null,"abstract":"<p><p>In brief: For externally fertilizing species, the composition of the fertilization environment, including the presence of female reproductive fluids, has a significant influence on sperm performance. This study integrated sperm performance analyses with transcriptomics to investigate the molecular basis of environment × gamete interactions and how ovarian fluid is associated with changes in sperm function and RNA profiles. Abstract: For most externally fertilizing teleost fish, sperm remain immotile until release into the external environment, where physicochemical cues trigger motility. In many species, including blue catfish (Ictalurus furcatus), ovarian fluid (OF) present at spawning further enhances sperm swimming performance, enabling more cells to swim longer and at higher velocities than in water alone. This study aimed to identify kinematic performance and RNA profiles associated with sperm-environment interactions under three conditions: (i) sperm activated with 25% OF and 75% deionized water (dH2O) (OF-enhanced); (ii) sperm combined with 100% OF (inactive); and (iii) sperm activated with 100% dH2O (control). We utilized computer-assisted sperm analysis (CASA) in combination with RNA-sequencing to examine sperm kinematics and transcript abundance between sperm environments. The CASA results showed that sperm activated in OF (OF-enhanced) had increased sperm motility and velocity compared with sperm activated in water alone (control). Variance component analysis further identified a male × female interaction associated with variation in sperm swimming performance. RNA-sequencing revealed distinct transcript abundance patterns among sperm environments and also revealed female-associated variation in transcript abundance, providing evidence for gamete level variation on sperm performance. Functional enrichment analysis further revealed that abundant transcripts in the OF treatments were associated with cytoskeletal organization, signaling, large multiprotein complexes, and membrane-associated processes. This study provides novel observations of OF-associated sperm RNA profiles and enhanced swimming performance in a teleost fish, generating hypotheses regarding underlying molecular mechanisms of sperm activation and reaction to environmental stimuli.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148907173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wearable sensors offer opportunities for phenotyping perinatal mood disorders: a narrative review.","authors":"Olimpia Carrioli, Lauryn Keeler Bruce, Saara M Kriplani, Tamar Schaap, Caden Stewart, Benjamin L Smarr","doi":"10.1093/reprod/xaag115","DOIUrl":"10.1093/reprod/xaag115","url":null,"abstract":"<p><p>In brief: The perinatal period represents a convergence of sleep disruption, circadian misalignment, and reproductive hormonal changes, which are the same biological axes implicated in depression and anxiety disorders. Analyses of wearable data complement clinical standards, capturing individual biological trajectories and identifying patterns that confer psychiatric risk. Abstract: Pregnancy and the postpartum period are known to elicit changes in sleep, circadian rhythms, and hormonal dynamics. The same biological axes are implicated in anxiety and depression in the general population. Given the high burden of perinatal mental health and the dire consequences of maternal wellbeing on the mother and infant alike, research differentiating those most at risk is a priority. Why some pregnancies experience these changes without causing mood disorders when many do is unclear but suggests there must be multiple types of disruption, some associated with more risk than others. Current standards of care rely on periodic, and often self-reported, assessments collected around scheduled visits, which can limit opportunities for early detection and timely intervention. We review evidence that wearable technology can complement the gold standard by offering continuous, high-resolution, personalized ecological monitoring of sleep, circadian rhythms, and hormonal dynamics at the individual level. These rich data, coupled with appropriate data science and machine learning techniques, enable early typing of pregnancies to support earlier diagnosis and intervention, with the potential for personalized insights, tailored treatment, and faster recovery.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148907144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and immunological mapping reveals peripubertal immune reprogramming in rat mammary gland development.","authors":"David Tovar Parra, Alec McDermott, Géraldine Delbès, Étienne Audet-Walsh, Martin Pelletier, Isabelle Plante","doi":"10.1093/reprod/xaag122","DOIUrl":"10.1093/reprod/xaag122","url":null,"abstract":"<p><p>In brief: The immune mechanisms accompanying normal postnatal mammary gland development remain incompletely understood. This study identifies peripuberty as a distinct phase of immune-stromal remodeling characterized by coordinated changes in immune cell signatures and chemokine signaling. Abstract: Postnatal mammary gland development is a hormone-dependent process involving extensive epithelial expansion and stromal remodeling. Although endocrine regulation of mammary morphogenesis is well established, the role of the immune system during normal mammary gland development remains incompletely understood. Here, we characterized immune remodeling across prepuberty, peripuberty, and adulthood in the rat mammary gland by integrating bulk transcriptomics, immune-stromal deconvolution, pathway enrichment analysis, cytokine and chemokine profiling, and immunolocalization. Our analyses identified peripuberty as a distinct phase of immune-stromal remodeling. Prepuberty was enriched in innate immune populations and immune establishment programs associated with tissue organization. In contrast, peripuberty displayed increased adaptive immune signatures and activation of chemokine pathways involved in leukocyte recruitment and spatial organization. Notably, this transition occurred alongside lower tissue concentrations of several inflammatory cytokines, suggesting that immune recruitment during peripuberty reflects a developmentally programmed signaling state rather than classical inflammation. By adulthood, immune composition and signaling profiles stabilized, consistent with immune homeostasis and surveillance. Transcript-protein integration revealed limited concordance between developmental changes in cytokine and chemokine transcripts and their corresponding proteins. No paired RNA-protein associated remained significant after multiple-testing correction, suggesting that regulation beyond transcription may contribute to soluble mediator profiles. Together, these findings demonstrate that postnatal mammary gland development is accompanied by stage-specific immune programming and establish a systems-level framework for understanding developmental immunology in the mammary gland.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}