Reproduction最新文献_第10页

Unlocking the Secrets of Reproductive Longevity: The Potential of Social Insects 揭开生殖长寿的秘密：社会性昆虫的潜力

IF 3.8 3区生物学

Reproduction Pub Date : 2024-04-01 DOI: 10.1530/rep-24-0020

María Fernanda Vergara-Martínez, Berenice Otero-Díaz, Ingrid Fetter-Pruneda

引用次数: 0

Spindle shape and volume differ in high- and low-quality metaphase II oocytes. 高质量和低质量的 II 期分裂卵母细胞中纺锤体的形状和体积不同。

IF 3.7 3区生物学

Reproduction Pub Date : 2024-03-19 Print Date: 2024-04-01 DOI: 10.1530/REP-23-0281

Monika Fluks, Robert Milewski, Szymon Tamborski, Maciej Szkulmowski, Anna Ajduk

{"title":"Spindle shape and volume differ in high- and low-quality metaphase II oocytes.","authors":"Monika Fluks, Robert Milewski, Szymon Tamborski, Maciej Szkulmowski, Anna Ajduk","doi":"10.1530/REP-23-0281","DOIUrl":"10.1530/REP-23-0281","url":null,"abstract":"In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the resulting embryos.Abstract: The proper development of embryos strongly depends on the quality of oocytes, so the evaluation of oocytes may be a useful initial step in IVF procedures. Additionally, it enables embryologists to make more informed decisions regarding the treatments chosen for the patients and better manage patients' expectations. Optical coherence microscopy (OCM) allows for non-invasive 3D visualization of intracellular structures, such as spindles or nuclei, which have been linked to the success of embryonic development. Here, we applied a mouse model to examine whether OCM imaging could be used in the quality assessment of metaphase II (MII) oocytes. We showed that quantitative parameters describing the shape and volume of the MII spindle were associated with the quality of the resulting embryos, including the likelihood of blastocyst formation and the embryos' ability to differentiate the trophectoderm and primitive endoderm, but not the epiblast. We also created a multivariate linear regression model, combining OCM-based quantification of MII spindles with morphokinetic analysis of the embryos, that allowed for improved evaluation of the embryo quality. Finally, we proved that OCM does not interfere with the viability of the scanned cells, at least during the preimplantation development. Therefore, we believe that OCM-based quantitative assessment of MII spindles can improve the oocyte and embryo selection in IVF procedures.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139944383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The N-terminal modification of HORMAD2 causes its ectopic persistence on synapsed chromosomes without meiotic blockade. HORMAD2 的 N 端修饰导致其异位持续存在于突触染色体上，而不发生减数分裂阻滞。

IF 4.3 3区生物学

Reproduction Pub Date : 2024-03-13 Print Date: 2024-04-01 DOI: 10.1530/REP-23-0330

Isabella G Cossu, N Adrian Leu, Yongjuan Guan, P Jeremy Wang

{"title":"The N-terminal modification of HORMAD2 causes its ectopic persistence on synapsed chromosomes without meiotic blockade.","authors":"Isabella G Cossu, N Adrian Leu, Yongjuan Guan, P Jeremy Wang","doi":"10.1530/REP-23-0330","DOIUrl":"10.1530/REP-23-0330","url":null,"abstract":"In brief: The dissociation of HORMA domain protein 2 (HORMAD2) from the synaptonemal complex is tightly regulated. This study reveals that the N-terminal region of HORMAD2 is critical for its dissociation from synapsed meiotic chromosomes.Abstract: During meiosis, homologous chromosomes undergo synapsis and recombination. HORMA domain proteins regulate key processes in meiosis. Mammalian HORMAD1 and HORMAD2 localize to unsynapsed chromosome axes but are removed upon synapsis by the TRIP13 AAA+ ATPase. TRIP13 engages the N-terminal region of HORMA domain proteins to induce an open conformation, resulting in the disassembly of protein complexes. Here, we report introduction of a 3×FLAG-HA tag to the N-terminus of HORMAD2 in mice. Coimmunoprecipitation coupled with mass spectrometry identified HORMAD1 and SYCP2 as HORMAD2-associated proteins in the testis. Unexpectedly, the N-terminal tagging of HORMAD2 resulted in its abnormal persistence along synapsed regions in pachynema and ectopic localization to telomeres in diplonema. Super-resolution microscopy revealed that 3×FLAG-HA-HORMAD2 was distributed along the central region of the synaptonemal complex, whereas wild-type HORMAD1 persisted along the lateral elements in 3×FLAG-HA-HORMAD2 meiocytes. Although homozygous mice completed meiosis and were fertile, homozygous males exhibited a significant reduction in sperm count. Collectively, these results suggest that the N-terminus of HORMAD2 is important for its timely removal from meiotic chromosome axes.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10993814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139944384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico-designed vitrification protocols: an approach to improve survival of in vitro produced bovine embryos 硅学设计的玻璃化方案：提高体外培育牛胚胎存活率的方法

IF 3.8 3区生物学

Reproduction Pub Date : 2024-03-01 DOI: 10.1530/rep-24-0036

Iris Martínez-Rodero, Judith Diaz-Muñoz, Adam Z. Higgins, Manel López Béjar, Teresa Mogas, Tania García-Martínez

{"title":"In silico-designed vitrification protocols: an approach to improve survival of in vitro produced bovine embryos","authors":"Iris Martínez-Rodero, Judith Diaz-Muñoz, Adam Z. Higgins, Manel López Béjar, Teresa Mogas, Tania García-Martínez","doi":"10.1530/rep-24-0036","DOIUrl":"https://doi.org/10.1530/rep-24-0036","url":null,"abstract":"The cryopreservation of in vitro produced (IVP) embryos is vital in the cattle industry for genetic selection and crossbreeding programs. Despite its importance, there is no standardized protocol yielding pregnancy rates comparable to fresh embryos. Current approaches often neglect the osmotic tolerance responses to cryoprotectants based on temperature and time. Hereby, we propose improved vitrification methods using shorter dehydration-based protocols. Blastocysts cultured for 7 (D7) or 8 days (D8) were exposed to standard equilibration solution (ES) at 25ºC and 38.5ºC. Optimized exposure times for each temperature and their impact in post-warming re-expansion, hatching rates, cell counts, and apoptosis rate were determined. In silico predictions aligned with in vitro observations, showing original volume recovery within 8min 30s at 25ºC or 3min 40s at 38.5ºC (D7 blastocysts) and 4min 25s at 25ºC and 3min 15s at 38.5ºC (D8 blastocysts) after exposure to ES. Vitrification at 38.5ºC resulted in D7 blastocysts re-expansion and hatching rates (93.1% and 38.1%, respectively) comparable to fresh embryos (100.0% and 32.4%, respectively), outperforming the 25ºC protocol (86.2% and 24.4%, respectively; P<0.05). No differences were observed between D7 and D8 blastocysts using the 38.5ºC protocol. Total cell number was maintained for D7 and D8 blastocysts vitrified at 38.5ºC but decreased at 25ºC (P<0.05). Apoptosis rates increased post-warming (P<0.05), except for D8 blastocysts vitrified at 38.5ºC, resembling fresh controls. In conclusion, based on biophysical permeability data, new ES incubation times of 3min 40s for D7 blastocysts and 3min 15s for D8 blastocysts at 38.5ºC were validated for optimizing vitrification/warming methods for bovine IVP blastocysts. ","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":"44 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140323004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomal or follicular FNDC3A decreases FOLR1 mRNA abundance, and progesterone and lactate synthesis in bovine granulosa cells 外泌体或卵泡 FNDC3A 会降低牛颗粒细胞中 FOLR1 mRNA 的丰度以及孕酮和乳酸的合成

IF 3.8 3区生物学

Reproduction Pub Date : 2024-03-01 DOI: 10.1530/rep-23-0451

Mathilde Daudon, Christelle Ramé, Christopher A Price, Joëlle Dupont

{"title":"Exosomal or follicular FNDC3A decreases FOLR1 mRNA abundance, and progesterone and lactate synthesis in bovine granulosa cells","authors":"Mathilde Daudon, Christelle Ramé, Christopher A Price, Joëlle Dupont","doi":"10.1530/rep-23-0451","DOIUrl":"https://doi.org/10.1530/rep-23-0451","url":null,"abstract":"Dairy cows go through a period of subfertility after parturition, triggered in part by a disruption of energy homeostasis. The mobilization of bodyfat alters the secretion of adipokines, which have been shown to impact ovarian function. Fibronectin type III domain-containing 3A (FNDC3A) is a recently discovered adipokine-myokine, and FNDC3A mRNA abundance in subcutaneous adipose tissue is increased post-partum in cattle. In this study, we hypothesized that FNDC3A may compromise granulosa cell function in cattle and investigated this using a well-established in vitro cell culture model. Here, we demonstrate the presence of FNDC3A protein associated with extracellular vesicles in follicular fluid and in plasma, suggesting an endocrine role for this adipokine. FNDC3A protein and mRNA was also detected in the bovine ovary (cortex, granulosa and theca cells, cumulus, oocyte and corpus luteum). Abundance of FNDC3A mRNA in granulosa cells from small follicles was increased by in vitro treatment with the adipokines leptin and TNFα but not by visfatin, resistin, adiponectin, chemerin or IGF1. Addition of recombinant FNDC3A at physiological doses (10 ng/ml) to granulosa cells decreased IGF1-dependent progesterone but not estradiol secretion and IGF1-dependent lactate secretion and abundance of GLUT3 and GLUT4 mRNA. This concentration of FNDC3A increased cell viability, abundance of mRNA encoding a putative receptor FOLR1, and increased phosphorylation of Akt. Collectively, these data suggest that FNDC3A may regulate folliculogenesis in cattle by modulating IGF1-dependent granulosa cell steroidogenesis and glucose metabolism.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":"51 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140166112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEK signalling pathway is required for hypoblast specification and migration in ovine MEK信号通路是绵羊低分化和迁移的必要条件

IF 3.8 3区生物学

Reproduction Pub Date : 2024-03-01 DOI: 10.1530/rep-24-0003

Nuria Martínez de los Reyes, Inés Flores-Borobia, Melissa Carvajal-Serna, Pilar Marigorta, Pablo Bermejo-Álvarez, Priscila Ramos-Ibeas

{"title":"MEK signalling pathway is required for hypoblast specification and migration in ovine","authors":"Nuria Martínez de los Reyes, Inés Flores-Borobia, Melissa Carvajal-Serna, Pilar Marigorta, Pablo Bermejo-Álvarez, Priscila Ramos-Ibeas","doi":"10.1530/rep-24-0003","DOIUrl":"https://doi.org/10.1530/rep-24-0003","url":null,"abstract":"Early embryo development requires the differentiation of three cell lineages in two differentiation events. The second lineage specification differentiates the inner cell mass into epiblast, which will form the proper foetus, and hypoblast, which together with the trophectoderm will form the extraembryonic membranes and the foetal part of the placenta. MEK signalling pathway is required for hypoblast differentiation in mouse embryos, but its role in ungulate embryos remains controversial. The aim of this work was to analyse the role of MEK signalling on hypoblast specification at the blastocyst stage, and on hypoblast migration during post-hatching stages in vitro in the ovine species. Using well-characterized and reliable lineage markers, and different MEK inhibitor concentrations, we demonstrate that MEK signalling pathway is required for hypoblast specification in the inner cell mass of the ovine blastocyst, and that it plays a role during the hypoblast migration occurring following blastocyst hatching. These results show that the role of MEK signalling pathway on hypoblast specification is conserved in phylogenetically distant mammals.\u0000","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":"64 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140322930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Naked mole-rat ovaries allow investigation of ovarian reserve in vitro germ cell expansion, and oocyte IVM within a single sample 裸鼹鼠卵巢可用于研究卵巢储备、体外生殖细胞扩增和单个样本中的卵母细胞 IVM

IF 3.8 3区生物学

Reproduction Pub Date : 2024-03-01 DOI: 10.1530/rep-23-0459

Gretchen M Rosado, Ana Martinez-Marchal, Mariela Faykoo-Martinez, Melissa M. Holmes, Miguel Angel Angel Brieño-Enriquez

引用次数: 0

Does TFAP2C govern conflicting cell fates in mouse preimplantation embryos? TFAP2C 是否控制着小鼠植入前胚胎中相互冲突的细胞命运？

IF 3.7 3区生物学

Reproduction Pub Date : 2024-02-21 Print Date: 2024-04-01 DOI: 10.1530/REP-23-0440

Chad S Driscoll, Jaehwan Kim, Mohamed Ashry, Jason G Knott

引用次数: 0

Decreased NSD2 impairs stromal cell proliferation in human endometrium via reprogramming H3K36me2. NSD2 减少会通过重编程 H3K36me2 影响人类子宫内膜基质细胞的增殖。

IF 3.7 3区生物学

Reproduction Pub Date : 2024-02-12 Print Date: 2024-03-01 DOI: 10.1530/REP-23-0254

Chuan-Mei Qin, Xiao-Wei Wei, Jia-Yi Wu, Xue-Qing Liu, Yi Lin

{"title":"Decreased NSD2 impairs stromal cell proliferation in human endometrium via reprogramming H3K36me2.","authors":"Chuan-Mei Qin, Xiao-Wei Wei, Jia-Yi Wu, Xue-Qing Liu, Yi Lin","doi":"10.1530/REP-23-0254","DOIUrl":"10.1530/REP-23-0254","url":null,"abstract":"In brief: The proliferation of the endometrium is regulated by histone methylation. This study shows that decreased NSD2 impairs proliferative-phase endometrial stromal cell proliferation in patients with recurrent implantation failure via epigenetic reprogramming of H3K36me2 methylation on the promoter region of MCM7.Abstract: Recurrent implantation failure (RIF) is a formidable challenge in assisted reproductive technology because of its unclear molecular mechanism. Impaired human endometrial stromal cell (HESC) proliferation disrupts the rhythm of the menstrual cycle, resulting in devastating disorders between the embryo and the endometrium. The molecular function of histone methylation enzymes in modulating HESC proliferation remains largely uncharacterized. Herein, we found that the levels of histone methyltransferase nuclear receptor binding SET domain protein 2 (NSD2) and the dimethylation of lysine 36 on histone H3 are decreased significantly in the proliferative-phase endometrium of patients with RIF. Knockdown of NSD2 in an HESC cell line markedly impaired cell proliferation and globally reduced H3K36me2 binding to chromatin, leading to altered expression of many genes. Transcriptomic analyses revealed that cell cycle-related gene sets were downregulated in the endometrium of patients with RIF and in NSD2‑knockdown HESCs. Furthermore, RNA-sequencing and CUT&Tag sequencing analysis suggested that NSD2 knockdown reduced the binding of H3K36me2 to the promoter region of cell cycle marker gene MCM7 (encoding minichromosome maintenance complex component 7) and downregulated its expression. The interaction of H3K36me2 with the MCM7 promoter was verified using chromatin immunoprecipitation-quantitative real-time PCR. Our results demonstrated a unifying epigenome-scale mechanism by which decreased NSD2 impairs endometrial stromal cell proliferation in the proliferative-phase endometrium of patients with RIF.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10895284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139493029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oocyte activation is a cytoplasm-confined event so far: what about the nucleus? 到目前为止，卵母细胞活化是一个细胞质封闭的事件，那么细胞核呢？

IF 3.7 3区生物学

Reproduction Pub Date : 2024-02-02 Print Date: 2024-03-01 DOI: 10.1530/REP-23-0360

Luisa Gioia, Luca Palazzese, Marta Czernik, Domenico Iuso, Helena Fulka, Josef Fulka, Pasqualino Loi

{"title":"Oocyte activation is a cytoplasm-confined event so far: what about the nucleus?","authors":"Luisa Gioia, Luca Palazzese, Marta Czernik, Domenico Iuso, Helena Fulka, Josef Fulka, Pasqualino Loi","doi":"10.1530/REP-23-0360","DOIUrl":"10.1530/REP-23-0360","url":null,"abstract":"The fertilizing spermatozoa induce a Ca2+ oscillatory pattern, the universal hallmark of oocyte activation, in all sexually reproducing animals. Assisted reproductive technologies (ARTs) like intracytoplasmic sperm injection (ICSI) bypass the physiological pathway; however, while a normal Ca2+ release pattern occurs in some species, particularly humans, artificial activation is compulsory for ICSI-fertilized oocytes to develop in most farm animals. Unlike the normal oscillatory pattern, most artificial activation protocols induce a single Ca2+ spike, undermining proper ICSI-derived embryo development in these species. Curiously, diploid parthenogenetic embryos activated by the same treatments develop normally at high frequencies and implant upon transfer in the uterus. We hypothesized that, at least in ruminant embryos, the oscillatory calcium waves late in the first cell cycle target preferentially the paternal pronucleus and are fundamentally important for paternal nuclear remodeling. We believe that Ca2+ signaling is central to full totipotency deployment of the paternal genome. Research in this area could highlight the asymmetry between the parental genome reprogramming timing/mechanisms in early development and impact ARTs like ICSI and cloning.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10895280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138808342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0