Reproduction最新文献_第10页

IF 3.7 3区生物学

Reproduction Pub Date : 2024-09-05 Print Date: 2024-10-01 DOI: 10.1530/REP-24-0081

Jiaojiao Huang, Xinyu Li, Dongyu Zhang, Luzhen Wang, Zhao Li, Zhenhua Song

{"title":"Depression-related testosterone deficiency is linked to reduced cholesterol levels in Leydig cells of CUMS mice.","authors":"Jiaojiao Huang, Xinyu Li, Dongyu Zhang, Luzhen Wang, Zhao Li, Zhenhua Song","doi":"10.1530/REP-24-0081","DOIUrl":"10.1530/REP-24-0081","url":null,"abstract":"In brief: Male reproductive problems under psychological stress were widely studied. Using chronically unpredictable mild stress-treated mice, we found that reduced serum testosterone levels were related to the low level of cholesterol in the Leydig cells.Abstract: Testosterone deficiency in humans can be caused by depressive symptoms; however, the causes of this deficiency are incompletely understood. This study demonstrates that male mice with depression-like symptoms due to chronic unpredictable mild stress (CUMS) show reduced serum testosterone levels and disrupted sexual behaviors. However, the observed testosterone reductions were not caused by apoptosis of Leydig cells. Oil red O staining revealed that lipid droplets were dramatically decreased in Leydig cells, suggesting that defects in cholesterol uptake might be related to testosterone deficiency in depression-like mice. To investigate the potential mechanism, lipid homeostasis was examined by liquid chromatography-tandem mass spectrometry. The results revealed that higher levels of sphingomyelins (SM 8:0;2O/28:1, 18:0;2O/22:2, 33:0;3O, 33:1;2O) were linked to decreased cholesterol levels. Further investigation indicated that testosterone biosynthesis from cholesterol in Leydig cells was impaired by the downregulation of Ldlr, Srb1, Lhr, and P450scc. Elevated levels of interferon signaling-associated pathways in depression-like mice testes may also contribute to decreased testosterone levels. Taken together, these findings provide a novel understanding of male reproductive problems under psychological stress and suggest that cholesterol uptake might be a causal factor in reduced testosterone production in depression-like mice.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The history of interferon-stimulated genes in pregnant cattle, sheep, and pigs. 妊娠牛、绵羊和猪的干扰素刺激基因 (ISG) 的历史。

IF 3.7 3区生物学

Reproduction Pub Date : 2024-09-04 Print Date: 2024-10-01 DOI: 10.1530/REP-24-0130

Gregory A Johnson, Fuller W Bazer, Robert C Burghardt, Heewon Seo, Guoyao Wu, Joe W Cain, Ky G Pohler

{"title":"The history of interferon-stimulated genes in pregnant cattle, sheep, and pigs.","authors":"Gregory A Johnson, Fuller W Bazer, Robert C Burghardt, Heewon Seo, Guoyao Wu, Joe W Cain, Ky G Pohler","doi":"10.1530/REP-24-0130","DOIUrl":"10.1530/REP-24-0130","url":null,"abstract":"In brief: The trophectoderm of the elongating conceptuses of cattle, sheep, and pigs secrete high amounts of interferons that increase or induce the expression of interferon-stimulated genes (ISGs) in the endometrium. Research concerning ISGs, performed from 1995 through 2023, is reviewed in this manuscript.Abstract: Expression of the classical interferon (IFN) stimulated genes (ISGs) increases in the endometrial stroma and glandular epithelium (GE) through activation of signal transducer and activator of transcription (STAT) signaling in response to the secretion of IFN tau (IFNT) and IFN gamma (IFNG) by the conceptuses of ruminants, including cattle and sheep, and pigs, respectively. The first of the classical ISGs to be characterized was ISG15 in cattle. Classical ISGs are not expressed by the endometrial luminal epithelium (LE) due to the expression of interferon regulatory factor 2 (IRF2) in the LE that prevents the expression of ISGs in the LE. Classical ISG expression in the endometrium serves as a reliable indicator of conceptus health and elongation in cattle. There are also nonclassical ISGs that are upregulated in endometrial LE in response to progesterone (P4) that are further stimulated by IFNT in sheep, the intracellular signaling pathway responsible for IFN effects on expression is unknown. ISGs are also upregulated in extrauterine tissues including CL and peripheral blood mononuclear cells (PBMCs). The expression of ISGs by the PBMCs of cattle serves as an early prognosticator of pregnancy. The physiological roles of ISGs remain obscure, but evidence suggests that they are at least in part involved in modifying the immune system to support endometrial remodeling necessary for the successful implantation of the conceptus. Our understanding of these ISGs is primarily the result of work from the laboratories of Drs Fuller Bazer, Thomas (Tod) Hansen, Gregory Johnson, Hakhyun Ka, Patrick Lonergan, Troy Ott, and Thomas Spencer.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Psychosocial Counseling of Transgender and Gender Diverse Individuals in Fertility and Reproductive Medicine: a Narrative Review 为生育和生殖医学中的变性者和性别多元化者提供社会心理辅导：叙述性综述

IF 3.8 3区生物学

Reproduction Pub Date : 2024-09-01 DOI: 10.1530/rep-24-0041

Peter Cummings, Angela K Lawson

{"title":"Psychosocial Counseling of Transgender and Gender Diverse Individuals in Fertility and Reproductive Medicine: a Narrative Review","authors":"Peter Cummings, Angela K Lawson","doi":"10.1530/rep-24-0041","DOIUrl":"https://doi.org/10.1530/rep-24-0041","url":null,"abstract":"Transgender and gender diverse (TGD) individuals experience significantly greater all-cause mortality and mental health disparities compared to their cisgender peers. Gender-affirming hormone therapy (GAHT) is a safe and effective treatment option for gender dysphoria that dramatically improves psychosocial health outcomes but may adversely impact fertility. Guidelines recommend medical fertility preservation (FP) counseling for TGD individuals and pre-fertility treatment psychoeducational implications consultation from qualified reproductive mental health professionals (MHPs) for TGD individuals pursuing FP or third-party reproductive treatment. However, sparce literature exists specific to the structure of mental health psychoeducational consultation for TGD individuals pursuing FP. \u0000This narrative review highlights aspects of mental health implications pre-fertility treatment consultation for the provision of supportive counseling. Results indicate that implications counseling should be conducted by an MHP with specialized training in reproductive mental health with TGD populations to reduce risk of harm and promote successful emotional navigation of fertility treatment. Such counseling should be psychoeducational and not gatekeeping in nature and may include consideration of the psychosocial (e.g., emotional, relational, ethical, spiritual, social) risks and benefits of various family building options. During these consultations, TGD individuals can explore their hopes and fears related to fertility and future family building plans and discuss realistic treatment expectations, individual strengths, coping and communications strategies, and identify key support network members which may aid in navigating the fertility treatment process. MHPs can provide referrals to appropriate resources if necessary to help TGD individuals navigate treatment while coping with psychological symptoms and promote behavior change. ","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":"3 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

REPRODUCTIVE AGEING: BGP-15 mitigates adverse impacts of aging on sperm quality, fertility, and offspring health in male mice 生殖衰老：BGP-15 可减轻衰老对雄性小鼠精子质量、生育能力和后代健康的不利影响

IF 3.8 3区生物学

Reproduction Pub Date : 2024-09-01 DOI: 10.1530/rep-24-0105

Macarena B Gonzalez, Carl A Campugan, Haley S Connaughton, Eryk Andreas, Yasmyn E Winstanley, Elisha J Williams, Camilla L Dorian, Sarah A Robertson, Cheryl Shoubridge, Rebecca L Robker

{"title":"REPRODUCTIVE AGEING: BGP-15 mitigates adverse impacts of aging on sperm quality, fertility, and offspring health in male mice","authors":"Macarena B Gonzalez, Carl A Campugan, Haley S Connaughton, Eryk Andreas, Yasmyn E Winstanley, Elisha J Williams, Camilla L Dorian, Sarah A Robertson, Cheryl Shoubridge, Rebecca L Robker","doi":"10.1530/rep-24-0105","DOIUrl":"https://doi.org/10.1530/rep-24-0105","url":null,"abstract":"<div>\u0000<h3>In Brief</h3>\u0000Aging in men is associated with diminished sperm quality and a higher incidence of altered fetal development and miscarriage in resultant pregnancies. This study in mice identifies a therapeutic compound that, when administered to aged males, improves sperm quality, subsequent embryo development and post-natal offspring health.\u0000</div>\u0000<div>\u0000<h3>Abstract</h3>\u0000Aging in men is associated with diminished sperm quality and a higher incidence of altered fetal development and miscarriage in resultant pregnancies. We used a mouse model of advanced paternal age to characterize embryonic development in older male mice and tested whether pre-conception treatment with the mitochondrial activator BGP-15 improves reproductive outcomes in old males. Like older men, reproductively old male mice had higher levels of sperm DNA damage and delayed pre-implantation development, associated with a reduced fetal weight and placental weight. Analysis of neonatal outcomes of in vivo-conceived offspring found that pups sired by old males were smaller, had delayed locomotor development, and increased mortality. BGP-15 treatment for 5 days prior to conception reduced sperm DNA oxidation levels and improved on-time embryo development after IVF and pup survival. BGP-15 treatment for 3 weeks prior to conception improved on-time pre-implantation embryo development and fetal viability and increased fetal size in pregnancies sired by old males. These results validate that ageing negatively affects male fertility and offspring physiology and indicates that pre-conception treatment with BGP-15 has the potential to improve sperm quality as well as early embryo development and post-natal health.\u0000</div>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":"21 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgender Medicine: CONTEXTUAL TRANS GYNECOLOGY 跨性别医学：跨性别妇产科对比

IF 3.8 3区生物学

Reproduction Pub Date : 2024-09-01 DOI: 10.1530/rep-24-0045

Mick A.a. van Trotsenburg

{"title":"Transgender Medicine: CONTEXTUAL TRANS GYNECOLOGY","authors":"Mick A.a. van Trotsenburg","doi":"10.1530/rep-24-0045","DOIUrl":"https://doi.org/10.1530/rep-24-0045","url":null,"abstract":"Transgender health care is not just gender-affirmative transitional care but committed to a superior objective, often beyond medical perspective: to create and maintain physical conditions for social functioning under the signs of the individually appropriate sex and to contribute to significantly reduce gender dysphoria. For these purposes it is a pre-requisite to have a distinct contextual understanding of the complex reality of trans people and knowledge about the numerous facettes of transgender healthcare.\u0000Gynecology for transgender and gender diverse people does not differ greatly from gynecology for cis gender female patients exept goals and context. Relief from complaints derived from genital organs is of course of importance but for transpeople there always is an overarching gender dimension sometimes complicating treatment and might give rise to misunderstandings. Also minority stress caused by societal factors frequently impacts the mental and physical state of health negatively and needs to be considered.\u0000This paper focusses on the context of trans gynecology and takes up various contentual aspects for both transmale patients having left genital organs in situ and for transfemale patients with gynecological demands. Gynecological topics are addressed, and how they are relevant for transgender and gender diverse people, from effects of supra- physiological androgen exposure on ovaries and uterus to vaginal bleeding and pelvic pain under testosterone treatment, from benign gynecological disorders as clinical manifestation may appear differently and treatment may be more burdensome to screening policies, and from reproductive issues to obstetrical care.\u0000","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":"15 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mind the Gap: A Nationwide Audit of LGBTQ+ Inclusion in Fertility Care Providers in the United Kingdom 注意差距：英国全国范围内对 LGBTQ+ 在生育护理机构中的融入情况进行的审计

IF 3.8 3区生物学

Reproduction Pub Date : 2024-09-01 DOI: 10.1530/rep-24-0173

Chloe He, Nour Al-ma'ani, Mei Francis, Jules Sales, Isabella Marson, Neringa Karpavičiūtė, Rishabh Hariharan, Ranya Derrick, Sotirios Saravelos, Luca Sabatini, Sofia Tzouganatou, Devika Nair, Danielle Ellis, Céline Jacques, Timothy Ferrand, Tash Oakes-Monger, Teodora Popa, Francisco Vasconcelos, Cristina Hickman

{"title":"Mind the Gap: A Nationwide Audit of LGBTQ+ Inclusion in Fertility Care Providers in the United Kingdom","authors":"Chloe He, Nour Al-ma'ani, Mei Francis, Jules Sales, Isabella Marson, Neringa Karpavičiūtė, Rishabh Hariharan, Ranya Derrick, Sotirios Saravelos, Luca Sabatini, Sofia Tzouganatou, Devika Nair, Danielle Ellis, Céline Jacques, Timothy Ferrand, Tash Oakes-Monger, Teodora Popa, Francisco Vasconcelos, Cristina Hickman","doi":"10.1530/rep-24-0173","DOIUrl":"https://doi.org/10.1530/rep-24-0173","url":null,"abstract":"LGBTQ+ patients comprise one of the fastest-growing user demographics in fertility care, yet they remain underrepresented in fertility research, practice, and discourse. Existing studies have revealed significant systemic barriers, including cisheteronormativity, discrimination, and gaps in clinical expertise. In this article, we present a checklist of measures clinics can take to improve LGBTQ+ inclusion in fertility care, co-created with members of the LGBTQ+ community. This checklist focuses on three key areas: cultural competence, clinical considerations, and online presence. The cultural competence criteria encompass inclusive communication practices, a broad understanding of LGBTQ+ healthcare needs, and knowledge of treatment options suitable for LGBTQ+ individuals. Clinical considerations include awareness of alternative examination and gamete collection techniques for transgender and non-binary patients, the existence of specific clinical pathways for LGBTQ+ patients, and sensitivity to the psychological aspects of fertility care unique to this demographic. The online presence criteria evaluate provider websites for the use of inclusive language and the availability of LGBTQ+-relevant information.\u0000The checklist was used as the foundation for an audit of fertility care providers across the UK in early 2024. Our audit identified a widespread lack of LGBTQ+ inclusion, particularly for transgender and non-binary patients, highlighting deficiencies in clinical knowledge and cultural competence. Our work calls attention to the need for further work to understand the barriers to inclusive and competent LGBTQ+ fertility care from both healthcare provider and patient perspectives. ","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":"416 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GRK2 is critical for the cleavage of the porcine embryo by regulating HSP90 and the AKT pathway. GRK2 通过调节 HSP90 和 AKT 通路，对猪胚胎的裂解至关重要。

IF 3.7 3区生物学

Reproduction Pub Date : 2024-08-27 Print Date: 2024-10-01 DOI: 10.1530/REP-23-0463

Dongjie Zhou, Xiao-Han Li, Song-Hee Lee, Ji-Dam Kim, Gyu-Hyun Lee, Jae-Min Sim, Xiang-Shun Cui

{"title":"GRK2 is critical for the cleavage of the porcine embryo by regulating HSP90 and the AKT pathway.","authors":"Dongjie Zhou, Xiao-Han Li, Song-Hee Lee, Ji-Dam Kim, Gyu-Hyun Lee, Jae-Min Sim, Xiang-Shun Cui","doi":"10.1530/REP-23-0463","DOIUrl":"10.1530/REP-23-0463","url":null,"abstract":"In brief: GRK2 deficiency disrupts the early embryonic development in pigs. The regulation of GRK2 on HSP90 and AKT may also play an important role during embryo development and tumor formation.Abstract: Among the family of GPCR kinases (GRKs) that regulate receptor phosphorylation and signaling termination, G-protein-coupled receptor kinase 2 (GRK2) binds to HSP90 in response to hypoxia or other stresses. In this study, we investigated the effects of GRK2 knockdown and inhibition on porcine embryonic development from the zygote stage. Immunofluorescence and western blotting were used to determine the localization and expression, respectively, of GRK2 and related proteins. First, GRK2 and p-GRK2 were expressed in both the cytoplasm and membrane and co-localized with HSP90 on the membrane. The mRNA level of GRK2 increased until the 8C-morula stage, suggesting that GRK2 may play an essential role during the early development of the porcine embryos. GRK2 knockdown reduced porcine embryo development capacity and led to significantly decreased blastocyst quality. In addition, inhibition of GRK2 also induced poor ability of embryo development at an early stage, indicating that GRK2 is critical for embryonic cleavage in pigs. Knockdown and inhibition of GRK2 reduced HSP90 expression, AKT activation, and cAMP levels. Additionally, GRK2 deficiency increased LC3 expression, suggesting enhanced autophagy during embryo development. In summary, we showed that GRK2 binds to HSP90 on the membrane to regulate embryonic cleavage and AKT activation during embryonic development in pigs.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a deep-learning model for detecting positive tubules during sperm recovery for nonobstructive azoospermia. 开发一种深度学习模型，用于检测非梗阻性无精子症患者精子复苏过程中的阳性小管。

IF 3.7 3区生物学

Reproduction Pub Date : 2024-08-27 Print Date: 2024-10-01 DOI: 10.1530/REP-24-0181

Teppei Takeshima, Jurii Karibe, Shinnosuke Kuroda, Yasushi Yumura

引用次数: 0

Chromosomal missegregation and aberrant embryo development in repro57 female mice with Rnf212 homozygous mutation. Rnf212同源突变的57代雌性小鼠的染色体错配和胚胎发育异常。

IF 3.7 3区生物学

Reproduction Pub Date : 2024-08-27 Print Date: 2024-10-01 DOI: 10.1530/REP-24-0030

Nanami Sono, Mone Takeshita, Mizuho Chikushi, Saki Nakashima, Shoko Miyawaki, Misaki Wakamatsu, Yasuhiro Fujiwara, Tetsuo Kunieda, Junko Otsuki

{"title":"Chromosomal missegregation and aberrant embryo development in repro57 female mice with Rnf212 homozygous mutation.","authors":"Nanami Sono, Mone Takeshita, Mizuho Chikushi, Saki Nakashima, Shoko Miyawaki, Misaki Wakamatsu, Yasuhiro Fujiwara, Tetsuo Kunieda, Junko Otsuki","doi":"10.1530/REP-24-0030","DOIUrl":"10.1530/REP-24-0030","url":null,"abstract":"In brief: Repro57 mice, bearing an Rnf212 gene mutation, exhibit infertility in both homozygous mutant males and females, revealing arrested spermatogenesis in males and investigating unclear mechanisms in females. The study highlights aneuploidy and altered kinetochore patterns in repro57 homozygous mutant oocytes, which impact later stages of embryo development.Abstract: Repro57 mice, induced with N-ethyl-N-nitrosourea and harboring a mutation in the Rnf212 gene, exhibit infertility in both homozygous mutant males and females. Rnf212 plays a crucial role in recombination and crossover designation. In male repro57 homozygous mutants, spermatocytes often degenerate during late prophase, and mature spermatozoa are absent in the seminiferous epithelium, indicating arrested spermatogenesis as the cause of infertility. Despite reports of infertility in Rnf212-knockout female mice, the specific mechanisms underlying infertility in female repro57 homozygous mutants remain elusive. This study investigates the chromosomal and kinetochore patterns of mature oocytes and their developmental potential following in vitro fertilization in female repro57 homozygous mutant mice. While all wild-type oocytes progress to metaphase II and exhibit euploidy, all repro57 homozygous mutant mouse oocytes display aneuploidy. Additionally, kinetochore distances in repro57 homozygous mutant oocytes exceed those observed in wild-type counterparts. Although no significant differences are noted in fertilization and early embryo development rates between wild-type and repro57 homozygous mutant mice, embryos derived from repro57 homozygous mutants exhibit significantly lower morula and blastocyst rates, accompanied by frequent cytokinesis failure and vacuole formation. These findings suggest that the premature segregation of sister chromatids in repro57 homozygous mutant mice adversely impacts the later stages of embryo development.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigations into the role of platelet-activating factor in the peri-conception period of the mare. 研究血小板活化因子（PAF）在母马围孕期的作用。

IF 3.7 3区生物学

Reproduction Pub Date : 2024-08-27 Print Date: 2024-10-01 DOI: 10.1530/REP-24-0049

Edwina F Lawson, Arnab Ghosh, Christopher Grupen, Jacob Netherton, Robert John Aitken, Nathan Druery Smith, Rebecca Lim, Hannah R Drury, Russell Pickford, Zamira Gibb, Mark Baker, Pradeep Singh Tanwar, Aleona Swegen

{"title":"Investigations into the role of platelet-activating factor in the peri-conception period of the mare.","authors":"Edwina F Lawson, Arnab Ghosh, Christopher Grupen, Jacob Netherton, Robert John Aitken, Nathan Druery Smith, Rebecca Lim, Hannah R Drury, Russell Pickford, Zamira Gibb, Mark Baker, Pradeep Singh Tanwar, Aleona Swegen","doi":"10.1530/REP-24-0049","DOIUrl":"10.1530/REP-24-0049","url":null,"abstract":"In brief: In many mammals, the lipid platelet-activating factor (PAF) has important functions in female reproduction and fertility. This study shows that PAF is present in the reproductive tissues of mares and is involved in processes related to ovulation and early pregnancy.Abstract: Platelet-activating factor (PAF) has been implicated in a number of reproductive processes ranging from ovulation to embryo motility but has not been widely explored in the mare. To identify the presence and examine the role of PAF in the equine periconception processes, targeted mass spectrometry coupled with chromatographic separation was performed on equine follicular fluid (FF), and PAF was quantitatively detected. Subsequently, untargeted high-resolution mass spectrometry-based lipidomic analysis was carried out to quantify PAF in different-sized pre-ovulatory follicles, whereby different molecular species of PAF, PAF (14:0) and PAF (16:1), were both seen to be increasing with follicle diameter. These findings suggest that PAF within FF is increasing as preovulatory follicles approach ovulation. Additionally, immunofluorescence staining identified the PAF receptor in the luminal pericellular, apical, and basal aspect of equine oviductal epithelial cells. Lastly, an equine oviductal epithelial organoid model was generated and showed that the addition of PAF significantly increased the ciliary beat frequency (CBF) (Hz), an action consistent with a role for PAF in embryo migration. It is proposed that the local action of PAF on the ciliated cells of the oviduct propels both the oocyte and the conceptus towards the uterus. In the mare, it appears that PAF is a contributor during the periconception period, potentially being a mediator in the mechanisms of ovulation and in the dialogue of very early pregnancy.","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0