Molecular Ecology最新文献

{"title":"Bumble Bees and Honey Bees on Islands Harbour Reduced Viral Species Richness, Yet Honey Bee Populations Are Dominated by a Deformed Wing Virus Recombinant.","authors":"Jana Dobelmann, Lena Wilfert","doi":"10.1111/mec.70070","DOIUrl":"https://doi.org/10.1111/mec.70070","url":null,"abstract":"<p><p>Pollinators harbour diverse RNA viromes that play a vital role in their health. Yet, factors that shape viral communities are often unclear. The European honey bee (Apis mellifera) is experiencing a viral epidemic since the emergence of the parasitic mite Varroa destructor (varroa) introduced vector-borne transmission, which has also been linked to increased viral spillover into wild pollinator communities. Varroa-free island populations provide natural laboratories to study the effect of varroa, while also allowing us to ask how islands affect viral communities. Barriers that restrict the dispersal of wild pollinators and their pathogens to islands may be overcome by human-mediated transport in managed honey bees. Here we used islands with and without varroa and matched mainland populations of honey bees (A. mellifera) and bumble bees (Bombus terrestris) from 2015 and 2021 to explore how varroa presence and island location affect the virome of managed and wild bees. We find lower viral richness on islands in both species. Bumble bees harbour a distinct viral community that was not affected by varroa but geographically structured. In honey bees, however, varroa-present populations contained more viral reads driven by a high abundance of deformed wing virus (DWV). Within the 6 years between the sampling events, DWV underwent a shift from mostly DWV-B towards a mix of DWV-B and recombinant strains. Surprisingly, these shifts appeared independent of varroa. Viewing pollinator virome composition within an ecological framework provides valuable insights into the barriers to virus spread and could help to predict drivers of disease emergence.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e70070"},"PeriodicalIF":3.9,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genealogical Analysis of Replicate Flower Colour Hybrid Zones in Antirrhinum.","authors":"Arka Pal, Daria Shipilina, Alan Le Moan, Adrian J McNairn, Jennifer K Grenier, Marek Kucka, Graham Coop, Yingguang Frank Chan, Nicholas H Barton, David L Field, Sean Stankowski","doi":"10.1111/mec.70067","DOIUrl":"https://doi.org/10.1111/mec.70067","url":null,"abstract":"<p><p>A major goal of speciation research is identifying loci that underpin barriers to gene flow. Population genomics takes a 'bottom-up' approach, scanning the genome for molecular signatures of processes that drive or maintain divergence. However, interpreting the 'genomic landscape' of speciation is complicated, because genome scans conflate multiple processes, most of which are not informative about gene flow. However, studying replicated population contrasts, including multiple incidences of secondary contact, can strengthen inferences. In this paper, we use linked-read sequencing (haplotagging), F_ST scans and genealogical methods to characterise the genomic landscape associated with replicate hybrid zone formation. We studied two flower colour varieties of the common snapdragon, Antirrhinum majus subspecies majus, that form secondary hybrid zones in multiple independent valleys in the Pyrenees. Consistent with past work, we found very low differentiation at one well-studied zone (Planoles). However, at a second zone (Avellanet), we found stronger differentiation and greater heterogeneity, which we argue is due to differences in the amount of introgression following secondary contact. Topology weighting of genealogical trees identified loci where haplotype diversity was associated with the two snapdragon varieties. Two of the strongest associations were at previously identified flower colour loci: Flavia, that affects yellow pigmentation, and Rosea/Eluta, two linked loci that affect magenta pigmentation. Preliminary analysis of coalescence times provides additional evidence for selective sweeps at these loci and barriers to gene flow. Our study highlights the impact of demographic history on the differentiation landscape, emphasising the need to distinguish between historical divergence and recent introgression.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e70067"},"PeriodicalIF":3.9,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secondary Decomposers Meet Their Predators: Decomposition Stage and Substrate Quality Jointly Structure Microbial Brown Food Webs During Fungal Necromass Decay","authors":"François Maillard,&nbsp;Briana H. Beatty,&nbsp;Stefan Geisen,&nbsp;Enrique Lara,&nbsp;Peter G. Kennedy","doi":"10.1111/mec.70060","DOIUrl":"10.1111/mec.70060","url":null,"abstract":"<p>Mycelial residues, also known as fungal necromass, represent a substantial fraction of soil organic matter (SOM) pools in terrestrial ecosystems worldwide. Although microbial decomposers are increasingly recognised as key drivers of fungal necromass carbon stock formation, the diversity and composition of their microbial predators—and the roles these predators play in mediating fungal necromass decomposition—have not been explored to date. To address this gap, we produced fungal necromass of varying biochemical quality from <i>Hyaloscypha bicolor</i> and decomposed it in forest topsoil in Minnesota, USA, to investigate how microbial decomposer (bacteria and fungi) and predator (protists and nematodes) communities differ between soil and necromass. We also examined whether microbial predators influence the abundance of fungal necromass decomposers and affect necromass decomposition rates. Over two sampling times (4 and 12 weeks), necromass exhibited rapid early mass loss followed by reduced decay, with a higher stabilised mass in high melanin necromass. Microbial abundances were higher in necromass than in surrounding soil, especially in low melanin necromass. Community composition of both decomposers and their predators differed between soil and necromass and shifted markedly with necromass quality and decomposition stage. Predator community composition was linked to bacterial and fungal abundances at both early and late stages of decay and was marginally associated with decomposition rates. We conclude that fungal necromass acts as a microbial ‘hotspot’ not only for decomposers but also for their predators. These findings highlight the importance of microbial predator–decomposer interactions to better understand the formation of fungal-derived SOM.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 18","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.70060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Detection of Missing Amphibians and Their Fungal Pathogen in a Biodiversity Hotspot Using eDNA.","authors":"Carla Martins Lopes, C Guilherme Becker, Délio Baêta, Juliane Petri de Carli Monteiro, Mariana Lúcio Lyra, Kelly Raquel Zamudio, Anthony Chariton, Célio Fernando Baptista Haddad","doi":"10.1111/mec.70075","DOIUrl":"https://doi.org/10.1111/mec.70075","url":null,"abstract":"<p><p>Since the 1970s, striking amphibian declines in population abundances and presumed extinctions have been recorded globally. The chytrid fungus Batrachochytrium dendrobatidis (Bd) is one of the key drivers of these declines. To investigate the potential role of Bd in the decline of Brazil's amphibians, we first used eDNA metabarcoding to survey for DNA traces of 10 threatened amphibian species from the Southern Brazilian Atlantic forest (SBAF). Using the eDNA samples of this survey, along with eDNA samples from previous surveys of 32 threatened amphibian species from the Northern Brazilian Atlantic forest (NBAF), we examined the relationships between the presence or absence of targeted amphibians with the presence or absence of Bd lineages. We detected DNA traces of two of the 10 targeted species in the survey of SBAF, including one species that was missing for over 40 years (Cycloramphus cedrensis). We also detected DNA traces of two Bd lineages (Bd-GPL and Bd-Brazil) at nine of the 12 sampling sites where community-level amphibian declines were first reported in the late 1970s. Our results support a post-panzootic scenario, where some threatened amphibian species are coping to persist in the presence of enzootic Bd. Our research also provides novel insights into simultaneous eDNA surveys targeting both hosts and pathogens, offering important methodological contributions to tropical ecology and conservation.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e70075"},"PeriodicalIF":3.9,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pesticide and Pathogen Exposure Causes Idiosyncratic Gene Expression Responses Across Four Diverse North American Bumble Bee Species","authors":"Rubén Martín-Blázquez,&nbsp;Sydney A. Cameron,&nbsp;Austin C. Calhoun,&nbsp;James P. Strange,&nbsp;Ben M. Sadd","doi":"10.1111/mec.70042","DOIUrl":"10.1111/mec.70042","url":null,"abstract":"<p>Bumble bee (<i>Bombus</i> Latreille) populations of certain species have declined precipitously in North America over several decades. Hypotheses for declines include exposure to the pathogen <i>Nosema bombi</i> and neonicotinoid pesticides. Importantly, populations of some bumble bee species remain stable despite their presumed exposure to these same stressors. We hypothesise that declining and stable species exhibit distinct responses to <i>N. bombi</i> and neonicotinoids, detectable as differential gene expression profiles. To test this, we exposed larvae of <i>Bombus occidentalis</i> (declining) and <i>B. impatiens</i> (stable) to <i>N. bombi</i> and to the neonicotinoid imidacloprid, plus a combination of both. RNA-seq analysis revealed almost no overlap between these species in gene expression responses to the individual stressors. There was more overlap of differentially expressed genes for the combined-stressor condition, but hundreds of genes still showed species-specific expression differences. To test whether the differential molecular responses could be associated with declining and stable species, we performed quantitative PCR on 20 selected genes, adding two additional species <i>B. terricola</i> (declining) and <i>B. griseocollis</i> (stable). These responses did not separate out by species decline status; each of the four species exhibit species-specific responses. Overall, these results highlight that generalising mechanisms and causes of decline across different species may be misleading, as diverse species respond molecularly in a species-specific manner to particular environmental stressors.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 17","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.70042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental DNA Comparative Phylogeography: Simultaneous Estimation of Population Structures Within a Species-Rich Group of Freshwater Gobies","authors":"Satsuki Tsuji,&nbsp;Shota Kunimatsu,&nbsp;Katsutoshi Watanabe","doi":"10.1111/mec.70059","DOIUrl":"10.1111/mec.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>Comparative phylogeography provides crucial insights into evolutionary processes shaping biodiversity patterns by analysing spatial genetic variations across multiple species. However, conventional capture-based methods are often labour-intensive, particularly for multi-species analyses. Environmental DNA (eDNA) analysis has significant advantages in comparative phylogeography, including simplified field surveys requiring only water collection and the potential to simultaneously analyse multiple species from a single sample. To further expand the eDNA application and demonstrate its utility in comparative phylogeographic studies, this study employed eDNA analysis to simultaneously analyse the phylogeographic patterns in a species-rich freshwater goby group (<i>Rhinogobius</i>) in the Japanese Archipelago. DNA amplification was performed on eDNA samples collected from 573 sites across the archipelago using newly designed group-specific primers targeting the mitochondrial cytochrome <i>b</i> region of <i>Rhinogobius</i>. High-throughput sequencing detected haplotypes of all nine known species (or species groups) occurring in this region, followed by phylogenetic and network analyses. The eDNA analysis successfully revealed the genetic population structures across multiple species. A landlocked species, <i>R. flumineus</i>, exhibited fine-scale population differentiation shaped by geomorphological barriers, while amphidromous species showed broader genetic patterns likely influenced by ocean currents and their ecological traits. The phylogenetic and phylogeographic patterns reconstructed by the eDNA analysis were almost completely concordant with previously identified patterns of limited groups based on conventional methods, demonstrating the reliability of eDNA-based comparative phylogeography. This study highlights the potential of eDNA to complement and partially replace conventional methods, facilitating large-scale comparative phylogeographic research to gain new insights into spatial patterns and evolutionary processes of biodiversity.</p>\u0000 </div>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 18","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opinion: Why Sex-Based Genomic Differentiation Should Not Be Overlooked in Population Genetics","authors":"Yu-Chi Chen,&nbsp;Nikolas Vellnow,&nbsp;Justin J. S. Wilcox,&nbsp;Sahar Javaheri Tehrani,&nbsp;Toni I. Gossmann","doi":"10.1111/mec.70061","DOIUrl":"10.1111/mec.70061","url":null,"abstract":"<p>Sex-specific genomic differentiation is a crucial yet frequently overlooked factor in population genetics. In this opinion piece, we leverage the substantial genomic resources available for the great tit (<i>Parus major</i>), including population-scale data sets from many European populations, to investigate genomic differentiation between males and females. Unlike in some other species, where high-quality genome assemblies exist but broad population sampling is lacking, the great tit offers a unique opportunity to study sex-based differentiation at both the genomic and population level. We identify significant differentiation at an autosomal locus on chromosome 5, which we hypothesise originates from sex-linked variation present on the sex chromosomes (Z and potentially W). By referencing genomic data from other songbirds with well-assembled sex chromosomes, we illustrate how autosomal loci may exhibit high sequence similarity to sex-linked regions. Our analyses demonstrate that uneven sex ratios in sampled populations can substantially bias differentiation metrics (e.g., <i>F</i>_<i>ST</i>), potentially resulting in false-positive interpretations of adaptive differentiation. To mitigate such issues, we stress the importance of sex-aware study designs, including balanced sex sampling and explicitly incorporating sex as a covariate. Furthermore, while optimal study designs would include high-quality reference genomes from both sexes, we recommend, as a pragmatic and cost-effective alternative for labs with limited resources, generating a reference genome from the heterogametic sex (females in birds) to ensure both sex chromosomes are represented in mapping and analysis. Finally, we emphasise the need for rigorous validation of candidate loci to ensure accurate and biologically meaningful outcomes in evolutionary genomic studies.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 18","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.70061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postglacial History of a Widespread Wood-Decaying Fungus in Europe Suggests Migration out of Multiple Refugia Followed by Admixture.","authors":"Dabao Sun Lu, Ine-Susanne Hopland Methlie, Jørn-Henrik Sønstebø, Anneli Andersen, David Peris, Sundy Maurice, Håvard Kauserud, Inger Skrede","doi":"10.1111/mec.70053","DOIUrl":"https://doi.org/10.1111/mec.70053","url":null,"abstract":"<p><p>While postglacial migration patterns have been well-studied in plants and animals in Europe, the same is not true for microorganisms such as fungi, and it remains unclear whether fungi have followed the same postglacial migration trajectories. In this study, we infer the postglacial history of the widespread wood-decay fungus Trichaptum abietinum in Europe. We investigate whether this fungus resided in multiple glacial refugia, as observed in many plants and animals, and how it migrated following the retreat of the ice. Population genomic analyses of T. abietinum suggest at least two glacial refugia in Europe: a southern refugium extending from Portugal to the Caucasus (potentially formed by several earlier distinct refugia), an eastern refugium and a potential third western refugium. Expansion from these refugia followed by migration and secondary contact has led to a complex genomic pattern of what we call the Mediterranean, Boreal, Atlantic and Central European admixed groups. From the southern and potential western refugia there have likely been northwards expansions resulting in the Mediterranean and Atlantic groups, while the Boreal group probably expanded westwards from the eastern refugium. A contact zone is present in Central Europe, where the Mediterranean and Boreal groups are admixing. In these admixed individuals, the second half of their largest scaffold is entirely inherited from the Boreal group, indicating either strong selection or genomic incompatibilities. In Scandinavia, the Atlantic and Boreal groups are in close contact but with limited admixture. Tajima's D and analyses of linkage disequilibrium decay support a recent history of contraction and expansion of this fungus in Europe. We conclude that the postglacial migration patterns in T. abietinum resemble those observed in several plants and animals, suggesting multiple glacial refugia followed by admixture during northward and westward expansions.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e70053"},"PeriodicalIF":3.9,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-Series RNA-Seq of Acropora tenuis Reveals Molecular Waves Leading to Synchronous Mass Spawning of Scleractinian Corals.","authors":"Yuki Yoshioka, Go Suzuki, Yuji Fujikura, Satokuni Tashiro, Taiga Uchida, Eiichi Shoguchi, Noriyuki Satoh, Chuya Shinzato","doi":"10.1111/mec.70054","DOIUrl":"https://doi.org/10.1111/mec.70054","url":null,"abstract":"<p><p>Although mass, synchronised spawning of scleractinian corals is a well-known phenomenon, its underlying molecular mechanisms, especially those that achieve synchronous release of gametes, are still unknown. In the genus Acropora, the dominant scleractinian coral in shallow reefs, spawning timing is synchronised in any given location, but often varies among locations. Here, we report gene expression cascades potentially driving synchronous mass spawning, revealed through transcriptome monitoring of Acropora tenuis, tracking both daily and monthly dynamics during a year-long experiment that included two spawning events. We identified 236 genes in four waves of molecular events that culminated in spawning. First, receptor function and prostaglandin secretion became active 2 weeks before spawning and gradually decreased, but remained elevated until spawning, suggesting communication of maturation among colonies. Second, 1-2 weeks before spawning, TGF-β signalling and spermiogenesis involving kinases were activated. Third, sperm capacitation and preparation of egg-sperm bundle material commenced a week before spawning. Finally, activation of transcription factor ELF1 triggered a signal cascade that induced spawning. This moonlight-independent system may serve to fine-tune the timing of spawning and may explain the broad geographic distribution and ecological success of Acropora, making it the most diverse and abundant genus of scleractinian corals in reef ecosystems.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e70054"},"PeriodicalIF":3.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Expressed Odorant Receptor Orthologs Detect the Aggregation Pheromone Lineatin in Trypodendron Ambrosia Beetles","authors":"Martin N. Andersson,&nbsp;Twinkle Biswas,&nbsp;Jothi Kumar Yuvaraj","doi":"10.1111/mec.70064","DOIUrl":"10.1111/mec.70064","url":null,"abstract":"<p>Chemical communication using pheromones is crucial for the reproductive success of many insect species, including beetles (Coleoptera). Pheromones are detected by specialised odorant receptors (ORs), called pheromone receptors (PRs), in the sensory neurons of the antennae. The PRs in Coleoptera remain understudied, with only a few receptors being functionally characterised. This limits our understanding of their response specificities and evolutionary origins. To this end, we aimed to identify PRs in two species of ambrosia beetles (Curculionidae; Scolytinae), the conifer-attacking <i>Trypodendron lineatum</i> (‘Tlin’) and the congener <i>T. domesticum</i> (‘Tdom’), specialising in deciduous trees. Both species use the female-produced aggregation pheromone lineatin for host- and mate finding. To date, no OR has been functionally characterised in ambrosia beetles. We specifically aimed to investigate the response specificity of the candidate PRs and their evolutionary relationships with PRs in other curculionids. We annotated 53 ORs from a <i>T. domesticum</i> antennal transcriptome, whereas ORs from <i>T. lineatum</i> were reported previously. We reveal numerous conserved <i>Trypodendron</i> OR orthologs with high sequence identity, with one orthologous pair (TdomOR13/TlinOR13) having much higher expression than all other OR genes. Through functional characterisation in HEK293 cells, we show that these receptors respond exclusively to lineatin. We further show that the receptors are phylogenetically well separated from all other known beetle PRs, including those in other curculionid species. This is the first study to demonstrate functional conservation of PR orthologs in congeneric beetles, and our findings support a scenario in which coleopteran PRs have evolved on multiple independent occasions.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":"34 18","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mec.70064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}