Research Journal of Biotechnology最新文献_第8页

Streptomyces sp. VITGV156 (MCC 4965), a quinoline producing Streptomyces 产喹啉链霉菌Streptomyces sp. VITGV156 (MCC 4965)

Research Journal of Biotechnology Pub Date : 2023-09-15 DOI: 10.25303/1810rjbt1970204

Amjad Hussain, Christopher J. Godwin

引用次数: 0

Molecular modeling study combined with deep learning approach for the identification of potent β-catenin inhibitors 结合深度学习方法的分子模型研究鉴定有效的β-catenin抑制剂

Research Journal of Biotechnology Pub Date : 2023-09-15 DOI: 10.25303/1810rjbt048059

Shanthi Veerappapillai, Shikhar Tandon

{"title":"Molecular modeling study combined with deep learning approach for the identification of potent β-catenin inhibitors","authors":"Shanthi Veerappapillai, Shikhar Tandon","doi":"10.25303/1810rjbt048059","DOIUrl":"https://doi.org/10.25303/1810rjbt048059","url":null,"abstract":"β-catenin is a propitious target for various cancer drugs for inhibiting tumour cell proliferation and differentiation. Even though several inhibitors have been discovered for β-catenin but its selectivity towards β-catenin and TCF-4 interactions is a major challenge. Hence, the expedition for identifying a selective drug for β-catenin inhibition against cancer will have immense potential and favour. The present study aims to scrutinise compounds that can impede β-catenin overexpression in cancer using an integrated pharmacophore and in silico docking-based screening of 28,007 molecules from the ZINC repository. The analysis yielded the top two compounds, namely ZINC000016051423 and ZINC000028564770, with better docking scores of -4.007 kcal/mol and -6.547 kcal/mol at the β-catenin binding pocket. Moreover, their free energy scores were -40.882 and -53.989 kcal/mol with favourable drug-likeness characteristics. Eventually, both hits exhibited better inhibitory activity against 66 colorectal cell lines using the PaccMann algorithm. In conclusion, our findings suggest that the lead compounds may serve as a possible β-catenin inhibitor during the treatment of cancer, though further experimental study is needed to evaluate the compound’s efficacy.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135486714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A critical review of the food colourants effects on Human Health and Environment 食用色素对人类健康和环境影响的综述

Research Journal of Biotechnology Pub Date : 2023-09-15 DOI: 10.25303/1810rjbt2490254

R. Jananipriya, S. Usha

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Microwave-assisted gelatin nanoparticles preparation: Green tea polyphenol mediated cross-linking and its enhanced bio-efficacy 微波辅助凝胶纳米颗粒制备:绿茶多酚介导的交联及其增强的生物功效

Research Journal of Biotechnology Pub Date : 2023-09-15 DOI: 10.25303/1810rjbt1640173

Karikalan Kulandaivelu, Shrila Banerjee, Abul Kalam Azad Mandal

引用次数: 0

Novel multi-epitope vaccine design against Mycobacterium tuberculosis: An Immunoinformatics strategy 新型多表位结核分枝杆菌疫苗设计:免疫信息学策略

Research Journal of Biotechnology Pub Date : 2023-09-15 DOI: 10.25303/1810rjbt060068

Dhayanitha Ranganathan Dhakshinamoorthy, Ramanathan Karuppasamy

{"title":"Novel multi-epitope vaccine design against Mycobacterium tuberculosis: An Immunoinformatics strategy","authors":"Dhayanitha Ranganathan Dhakshinamoorthy, Ramanathan Karuppasamy","doi":"10.25303/1810rjbt060068","DOIUrl":"https://doi.org/10.25303/1810rjbt060068","url":null,"abstract":"Vaccination has proven to be an effective strategy for the prevention of tuberculosis (TB). Interestingly, peptide-based vaccines that elicit a specific immunological response are currently being explored as alternatives to the BCG vaccine. Thus, the present study aimed to design a novel, efficacious peptide-based vaccine against tuberculosis targeting Rv1115, a membrane protein and a potent stimulator of INF-γ. Initially, the immunodominant CD4+, CD8+ and B cell epitopes of Rv1115 were identified and scrutinized based on their propensity to evoke immunological responses. The propitious epitopes were then combined using the appropriate linkers (EAAAK, AAY, KK and GPGPG) and adjuvant (CobT) for the chimeric vaccine design. Further, the designed chimeric vaccine was subjected to 3D structure modelling, refinement and validation. Finally, the modelled vaccine construct was used for protein–protein docking studies with toll-like receptors 3 and 4 (TLR-3 and TLR-4) followed by immune simulation analysis and in silico cloning. Overall, the immunoinformatic results suggest that the developed chimeric vaccine could elicit robust immune responses and can be employed as an efficient preventative therapy for tuberculosis.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135486569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation of computational strategies to combat drug resistance in Mycobacterium tuberculosis 实施计算策略以对抗结核分枝杆菌的耐药性

Research Journal of Biotechnology Pub Date : 2023-09-15 DOI: 10.25303/1810rjbt01008

Mrunalini Junghare, Sejal Jain, Ramanathan Karuppasamy

{"title":"Implementation of computational strategies to combat drug resistance in Mycobacterium tuberculosis","authors":"Mrunalini Junghare, Sejal Jain, Ramanathan Karuppasamy","doi":"10.25303/1810rjbt01008","DOIUrl":"https://doi.org/10.25303/1810rjbt01008","url":null,"abstract":"Mycobacterium tuberculosis (MTB) is an infectious pathogen that causes tuberculosis (TB). Even though it is curable, the first-line anti-TB medication rifampicin has been a source of rising concern for human health across the globe. However, rifampicin resistance results from mutations in the RNA polymerase's binding site of the target protein. This infectious pathogen can survive in macrophages and can induce a long-lasting latent infection. The prolonged survival of mycobacterial species was achieved with the aid of serine/threonine protein kinase G (PknG) which performs a preventive role in this situation. Thus, PknG is believed to be an essential target to prevent the pathogen from entering the latency stage. The present investigation was oriented towards molecular-based virtual screening carried out using AutoDock for the FDA-approved and nutraceutical subsets of compounds from the DrugBank repository. Initially, a total of 3035 compounds were screened based on their absorption, distribution, metabolism, excretion and toxicity (ADMET) properties. The screened molecules were taken for docking purposes after which post-docking analysis was performed by calculating the random forest (RF) score. Further, the structural characterization of hit compounds was validated using interaction studies and in silico bioactivity prediction studies. Finally, the anti-myotubular activity prediction was carried out in the search for potential inhibitors. Importantly, four potential ligands, namely DB00080, DB00807, DB06249 and DB06274 were preferred after a thorough investigation. These ligands showed significant structural and protein-ligand interactions, suggesting that they might be potentially used as therapeutic candidates to combat MTB.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135486713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicological implications of arecoline N- oxide in vivo in mice test system 槟榔碱N-氧化物在小鼠体内试验系统的毒理学意义

Research Journal of Biotechnology Pub Date : 2023-08-15 DOI: 10.25303/1809rjbt0110

Aparajita Das, Sarbani Giri, Abhijit Mandal, Malaya Ghosh, Sudip Choudhury

{"title":"Toxicological implications of arecoline N- oxide in vivo in mice test system","authors":"Aparajita Das, Sarbani Giri, Abhijit Mandal, Malaya Ghosh, Sudip Choudhury","doi":"10.25303/1809rjbt0110","DOIUrl":"https://doi.org/10.25303/1809rjbt0110","url":null,"abstract":"Arecoline N- oxide has surfaced as a probable key player of areca nut induced maladies. However, information on the adverse effects of arecoline N- oxide in-vivo is lacking. Mice were divided into 3 groups; the control group was injected with normal saline intraperitoneally while the treatment groups were injected with arecoline N- oxide and arecoline respectively at a dosage of 20 mg/ kg body weight intraperitoneally for thirty days. At the end of the exposure period, end points indicative of toxicity were investigated. Arecoline N- oxide was found to induce DNA damage in mice bone marrow cells. Observable changes in histology of liver indicated arecoline N- oxide to be hepatotoxic. Histological studies also showed arecoline N- oxide to induce infiltration of mononuclear cells in the kidneys. Oxidative stress was induced by arecoline N- oxide in both liver and kidneys as indicated by increased level of lipid peroxidation and decreased level of glutathione, superoxide dismutase and catalase in the tissues. The present study showed arecoline N- oxide to have genotoxic, hepatotoxic and mild nephrotoxic effects in mice which might be due to generation of oxidative stress.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Silico Analysis and Docking Studies on Cystic Fibrosis to identify Potential Drug Candidates 囊性纤维化的计算机分析和对接研究，以确定潜在的候选药物

Research Journal of Biotechnology Pub Date : 2023-08-15 DOI: 10.25303/1809rjbt2590270

Nishita Verma, Abhishek Sengupta, Seema Bhatnagar, Priyanka Narad

{"title":"In Silico Analysis and Docking Studies on Cystic Fibrosis to identify Potential Drug Candidates","authors":"Nishita Verma, Abhishek Sengupta, Seema Bhatnagar, Priyanka Narad","doi":"10.25303/1809rjbt2590270","DOIUrl":"https://doi.org/10.25303/1809rjbt2590270","url":null,"abstract":"Cystic fibrosis (CF) is a multi-system autosomal recessive disorder due to defects in the CF transmembrane conductance regulator (CFTR) protein, most commonly affecting the lungs. Despite being thought of as an uncommon ailment, cystic fibrosis (CF) is the most common monogenic sickness among those who are mostly of European descent. It is estimated that 1% of people worldwide have a single faulty copy of the CFTR gene. A recent pilot study using NGS technology in 2020 from Delhi’s Sir Ganga Ram Hospital, indicated the prevalence of cystic fibrosis mutations of 1 in 2,000 persons. This is much higher than the earlier estimated prevalence as the disease was rare in the Indian population. The most common mutations are delta F 508 and G551D which are also prevalent in the Indian population. The use of computer-aided drug design (CADD) can reduce the time needed for the discovery, evaluation and structure-optimization of new therapeutic candidates. CADD may be advantageous for pharmaceuticals with logical designs. Multiple breakthroughs have been made in the study of small molecule medicines and natural chemicals for the treatment of cystic fibrosis. Seven well-known small-molecule medications that target the CFTR were chosen including Ivacaftor, Lumacaftor, Tezacaftor, Galicaftor, Olacaftor, Navocaftor and Elexacaftor. The natural compounds chosen were Genistein, Curcumin and Resveratrol. They were all docked onto the CFTR target protein and the effectiveness of the therapy was evaluated based on the docking scores. It was also planned to investigate if the combined impacts of these two different kinds of molecules may help in the development of new medications. To achieve this, combinatorial docking was tried using the small molecule market medication and the natural chemical that showed the best interaction with the target protein. Finally, based on molecular docking studies, Lonidamine, a well-known medicinal molecule was used to determine whether it may target cystic fibrosis.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk Assessment of Human Subjects occupationally exposed to Extremely Low Frequency Electromagnetic Fields (ELF-EMFs) and Light at Night (LAN) with particular reference to Melatonin Hypothesis 人类受试者职业暴露于极低频电磁场(ELF-EMFs)和夜间光(LAN)的风险评估，特别是参考褪黑激素假说

Research Journal of Biotechnology Pub Date : 2023-08-15 DOI: 10.25303/1809rjbt2050215

Vemula Surender, Kavitha Varak, Ravindra Babu Potti, R. Tiwari

{"title":"Risk Assessment of Human Subjects occupationally exposed to Extremely Low Frequency Electromagnetic Fields (ELF-EMFs) and Light at Night (LAN) with particular reference to Melatonin Hypothesis","authors":"Vemula Surender, Kavitha Varak, Ravindra Babu Potti, R. Tiwari","doi":"10.25303/1809rjbt2050215","DOIUrl":"https://doi.org/10.25303/1809rjbt2050215","url":null,"abstract":"Modern society makes pervasive use of electric power producing electromagnetic Fields (EMFs) and light at night (LAN). This has resulted in the indiscriminate use of electrical and electronic gadgets which release electromagnetic radiations which are supposed to cause the biological effects by some scientists. However, ELF- EMFs and LAN are hypothesized to be responsible for the changes in hormonal configurations leading to development of cancer in women particularly nurses who are occupationally exposed to ELF- EMFs and LAN. The blood samples of 342 exposed subjects and 150 non exposed individuals were analyzed. Plasma melatonin was measured by radioimmunoassay (RIA). DNA damage was studied by alkaline comet assay along with micronucleus test and RT-PCR. Our results suggest that the plasma melatonin levels were significantly suppressed in the occupationally exposed subjects (p < 0.05) and DNA damage ranged between 8μm to 10μm. Group-C exposed subjects showed more DNA damage. The occupationally exposed subjects were found to be vulnerable for electromagnetic stress with decreased melatonin concentrations and increased DNA damage.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progression of Type 2 Diabetic Nephropathy and Serum Monocyte Chemoattractant Protein-1 (MCP-1) Levels 2型糖尿病肾病的进展和血清单核细胞趋化蛋白-1 (MCP-1)水平

Research Journal of Biotechnology Pub Date : 2023-08-15 DOI: 10.25303/1809rjbt1320136

S. Mohammed Ahmed, H. Al-Saeed Hassan, Sami Malik Arif, Ali Alhassnawi Muqdam, S. Khlaif Mohammed, Abdulla Qassim Ali

{"title":"Progression of Type 2 Diabetic Nephropathy and Serum Monocyte Chemoattractant Protein-1 (MCP-1) Levels","authors":"S. Mohammed Ahmed, H. Al-Saeed Hassan, Sami Malik Arif, Ali Alhassnawi Muqdam, S. Khlaif Mohammed, Abdulla Qassim Ali","doi":"10.25303/1809rjbt1320136","DOIUrl":"https://doi.org/10.25303/1809rjbt1320136","url":null,"abstract":"Diabetic nephropathy is an important complication that develops from diabetes mellitus. The chemotactic cytokine Monocyte Chemoattractant Protein-1 (MCP-1) exhibits selective binding and activation of the C-C motif Chemokine Receptor 2 (CCR2). MCP-1 has been consistently observed to play a role in the pathogenesis of chronic and diabetic renal disease in clinical settings, regardless of the different stages and phenotypes. The main goal of this study was to assess and contrast the levels of monocyte chemoattractant protein-1 (MCP-1/CCL2) in individuals with diabetic nephropathy and those who show healthy state to examine the possibility of the usefulness of MCP-1/CCL2 as a biomarker of prognosis for early identification in patients developing albuminuria. The study involved the study of a sample of ninety patients diagnosed with type 2 diabetic nephropathy, while a control group of thirty individuals was also included for comparison purposes. The study recruited participants requesting from Baghdad and Maysan in Iraq. Urine specimens were obtained to determine the urinary protein concentration. Samples of blood and serum were collected to measure the levels of serum creatinine (s-Cr), fasting plasma glucose (F.P.G.), glycohemoglobin A1c (Hba1c) and serum MCP-1. The results of the statistical analysis indicate that there were significant differences (P < 0.001) in the levels of MCP-1 between the microalbuminuric (stage 2) and macroalbuminuric (stage 3) when compared with the control group and between the stage 2 and stage 3 groups. Based on these findings, it can be concluded that MCP-1 levels may serve as biochemical indicators for disease progression of D.N.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0