Research Journal of Biotechnology最新文献_第10页

Heterologous Expression, Purification and Characterization of Fusion-type Pediocin PA-1 in Escherichia coli 融合型Pediocin PA-1在大肠杆菌中的异源表达、纯化及特性研究

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt1450152

P. Nguyen, Thi Hong Thuy Dao, Van Hau Nguyen, Phuong Thao Dang Thi, Hieu Nguyen

{"title":"Heterologous Expression, Purification and Characterization of Fusion-type Pediocin PA-1 in Escherichia coli","authors":"P. Nguyen, Thi Hong Thuy Dao, Van Hau Nguyen, Phuong Thao Dang Thi, Hieu Nguyen","doi":"10.25303/1808rjbt1450152","DOIUrl":"https://doi.org/10.25303/1808rjbt1450152","url":null,"abstract":"Pediocin PA-1 is a 4.6 kDa bacteriocin that shows strong antimicrobial activity against some Gram-positive pathogens such as Listeria monocytogenes and Enterococcus faecalis. Due to broad inhibitory spectrum as well as pH and temperature stability, pediocin has a potential application in food preservation as well as in the pharmaceutical industry. For higher manufactory efficiency, pediocin has been expressed in some other heterologous expression systems, mostly on Escherichia coli with different strategies. Here, we show a new strategy to produce pediocin from E. coli BL21(DE3) system as a fusion form by using the expression vector pET43.1a which contains the NusA tag. Our results showed that NusA-fused pediocin almost presented in soluble form with high efficiency (79.8 mg/L obtained by Ni-NTA purification). After removing the fusion tag, recombinant pediocin showed antimicrobial activity against L. monocytogenes ATCC 13932 as 23.5×103 AU/mg of specific activity as well as against E. faecalis, Lactobacillus plantarum and Streptococcus thermophilus. Recombinant pediocin was stable at the wide range of pH (1-12 for 60 min) and high temperature (100oC for 15 min) as well as sensitive to protease treatment as the nature pediocin. These characteristics have opened prospects for applying pediocin as a biological preservative in the food industry.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46333999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant diversity in a self-regenerating mangrove after disturbance by Durian typhoon in Ho Chi Minh City, Vietnam 台风榴莲干扰下越南胡志明市自再生红树林的植物多样性

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt032038

Dien Tran Quang, Huy Pham Xuan, Hieu Tram Trung, Huong Pham Quynh, Thi Nguyen Thi Lan

{"title":"Plant diversity in a self-regenerating mangrove after disturbance by Durian typhoon in Ho Chi Minh City, Vietnam","authors":"Dien Tran Quang, Huy Pham Xuan, Hieu Tram Trung, Huong Pham Quynh, Thi Nguyen Thi Lan","doi":"10.25303/1808rjbt032038","DOIUrl":"https://doi.org/10.25303/1808rjbt032038","url":null,"abstract":"The study aims to assess the plant diversity in a self-regenerating mangrove in Can Gio mangrove biosphere reserve after being offended by Durian typhoon in 2006. Tree individual numbers were collected in permanent sample plots which were placed in the intact zone (INT), transitional zones (TRA) and devastated zones (DEV), designed in 2007 to monitor the mangrove succession. Diversity was examined via diversity indices including tree density (D), Shannon-Wiener (H'), Evenness (E) and Simpson's Dominance index (Cd). The species numbers in the INT, TRA and DEV sections were 3, 5 and 10 respectively. The highest tree density of Rhizophora apiculata Blume was acquired in the INT and TRA zone while Avicennia spp. was the dominant species in the DEV zones. The H' and Cd indices revealed a significant difference between the DEV zone and the others in terms of species diversity. Avicennia spp. was widely distributed in the devastated zone. On the other hand, their appearance frequency in the TRA zone was low, but these species were dominant in the DEV zone. After 13 years of self-recovery, the devastated mangrove was turning into a multi-species community while the transitional zone tended to sustain the monoculture structure.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41884511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on induction of Allium sativum L. hairy roots with antibacterial activity against Vibrio 大蒜毛状根对弧菌抑菌的诱导研究

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt1530162

Bach Phuong Vu Thi, P. Nguyen, Phuoc Quy Thang Nguyen, Phi Tran, Diem Phuong Quach Ngo

{"title":"Study on induction of Allium sativum L. hairy roots with antibacterial activity against Vibrio","authors":"Bach Phuong Vu Thi, P. Nguyen, Phuoc Quy Thang Nguyen, Phi Tran, Diem Phuong Quach Ngo","doi":"10.25303/1808rjbt1530162","DOIUrl":"https://doi.org/10.25303/1808rjbt1530162","url":null,"abstract":"Allium sativum L. has long been used to improve the health of humans and other species due to its antibacterial activity. Applying biotechnology to create a stable and large-scale production process for Allium sativum L., medicinal herbs by hairy root culture technology are essential. However, Allium sativum L. is a monocotyledonous plant, so its ability to induce hairy roots is lower than that of dicotyledonous plants, which requires more research investment. In this study, we succeeded in inducing hairy roots in monocotyledonous plants Allium sativum L, by evaluating parameters impacting hairy root induction such as plant organs, acetosyringone concentration, infection time and co-culture time. The results showed that the optimal procedure for induction of Allium sativum L. hairy roots by Agrobacterium rhizogenes ATCC 15834 was as follows: 10 day old in vitro Allium sativum L. sprouts were wound and soaked in 100 μM acetosyringone solution for 10 min, infection time was 5 minutes, co-culture time was 48 hours cultured on B5 medium (Gamborg B5 medium) having the induction rate of hairy roots reaching 64.4% after 14 days of culture. Allium sativum L. hairy roots had good antibacterial effect against all three strains of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus because the MBC/MIC ratios are all less than or equal to 2. Especially, for V. cholerae (MIC = 1.84 mg/ml, MBC = 2.11 mg/ml), hairy roots have antibacterial activity equivalent to tetracycline antibiotics and better than non-infectious roots.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42105121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation on the biological activity of the leafy liverwort Denotarisia linguifolia (De Not.) Grolle 叶苔生物活性的研究Grolle

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt1630171

Phan-Duy Nguyen-Nhien, Nguyen-Vo Ky-Duyen, T. Tan, Quach-Ngo Diem-Phuong

{"title":"Investigation on the biological activity of the leafy liverwort Denotarisia linguifolia (De Not.) Grolle","authors":"Phan-Duy Nguyen-Nhien, Nguyen-Vo Ky-Duyen, T. Tan, Quach-Ngo Diem-Phuong","doi":"10.25303/1808rjbt1630171","DOIUrl":"https://doi.org/10.25303/1808rjbt1630171","url":null,"abstract":"Denotarisia linguifolia (De Not.) Grolle, or D. linguifolia, collected from LangBiang was used to make fraction extracts used for the evaluation of biological activity. Four extracts (n-hexane, chloroform, ethyl acetate and ethanol) were assessed for antioxidant (ferric reducing power, DPPH and lipid peroxidation), enzymatic inhibition (tyrosinase, α-glucosidase and hyaluronidase), cytotoxicity against MCF-7 and phytochemistry screening. For antioxidant assays, ethyl acetate extract had the highest antioxidant capacity ferric reducing power and DPPH scavenging, while chloroform extract had the highest antioxidant capacity in lipid peroxidation. For enzyme inhibition evaluation, D. linguifolia ability in anti-tyrosinase was insignificant while its ability in anti-α-glucosidase and anti-hyaluronidase was exceptional, chloroform extract had the best ability against α-glucosidase and ethyl acetate extract had the best ability against hyaluronidase. Unexpectedly, the n-hexane extract had the best potential for preventing the proliferation of MCF-7. Finally, the phytochemistry screening revealed the presence of important secondary metabolites within the liverwort extracts such as phenolic compounds (phenols, quinones, coumarins and flavonoids) and glycosides (saponins and cardiac glycosides).","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42147256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro antagonistic effect of Bacillus licheniformis D7 against Colletotrichum siamense Pathogenic to Mango 地衣芽孢杆菌D7对芒果炭疽病菌的体外拮抗作用

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt082088

Pham Tan-Viet, Pham Dong-Trieu, Nguyen Ngoc-An, Nguyen Hanh Thi-Dieu

{"title":"In vitro antagonistic effect of Bacillus licheniformis D7 against Colletotrichum siamense Pathogenic to Mango","authors":"Pham Tan-Viet, Pham Dong-Trieu, Nguyen Ngoc-An, Nguyen Hanh Thi-Dieu","doi":"10.25303/1808rjbt082088","DOIUrl":"https://doi.org/10.25303/1808rjbt082088","url":null,"abstract":"The genus Bacillus includes endospore-producing Gram-positive bacteria, most of which are harmless to animals and plants while known for their ability to inhibit a variety of harmful bacteria and molds. Colletotrichum is one of the common causative agents of anthracnose in mangoes and other crops, causing great economic losses in agriculture. In order to provide a potential and eco-friendly solution for the control of the disease caused by Colletotrichum siamense, antifungal characteristics of B. licheniformis D7 culture were investigated. The results showed that B. licheniformis D7 culture contained extracellular enzymes such as chitinase, protease, cellulase and displayed the ability to deform and decompose the fungal mycelia. At high temperatures of 70℃-80℃ for 15 minutes, the culture showed residual antifungal activity of 44.4%-61.1% and remained at 27.8% when treated at 90℃. In addition, the activity remained more than 81% after 2 hours of incubation in a wide range of pH from 4.0 to 9.0. In the mango model, the culture of strain D7 showed high antagonistic effect against C. siamense and reduced anthracnose severity by more than 98.4%. Overally, B. licheniformis D7 can be considered as potential antifungal biocontrol agent of anthracnose caused by C. siamense in mango and other fruits, effectively contributing to the future sustainable agriculture.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41537922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The change of zooplankton communities in three areas for co-management of coastal fisheries in Can Gio, Ho Chi Minh City 胡志明市Can Gio沿海渔业共同管理三个地区浮游动物群落的变化

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt1060112

My Tran Ngoc Diem, Ha Tran Le Quang, Nhi La Duong Song

{"title":"The change of zooplankton communities in three areas for co-management of coastal fisheries in Can Gio, Ho Chi Minh City","authors":"My Tran Ngoc Diem, Ha Tran Le Quang, Nhi La Duong Song","doi":"10.25303/1808rjbt1060112","DOIUrl":"https://doi.org/10.25303/1808rjbt1060112","url":null,"abstract":"Zooplankton plays a role as a living food source in the food web of water bodies, determining the growth and development of many aquatic species, especially at the larval stage. The study has recorded 52 species of 29 genera, 21 families, 6 classes, 4 phylum and 7 types of larvae in 28 sampling sites of 3 areas for co-management of coastal fisheries. The density of plankton individuals ranged from 49300 individuals/m3 to 272812 individuals/ m3. The number of species and density of plankton individuals in Thanh An area is higher than in the other areas (Long Hoa and Can Thanh). The similarity of the zooplankton communities showed that at 40% of the sampling sites, they were divided into 2 groups as follows: group 1 of water bodies in the co-management model area in Long Hoa and Can Thanh, group 2 included the water bodies of Thanh An area. The Copepoda class is the highest proportion in the sampling sites, showing that it will be an abundant food source for the growth and development of aquatic species that are cultured or live naturally in this ecosystem. It is also an important biological factor that needs to be managed and conserved to create a natural habitat for the sustainability of fish stocks in the area.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41943124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Endophytic Bacteria from Cavendish Banana pseudostem (Musa acuminata) and Plant Growth Promoting Characterization 卡文迪什香蕉假茎内生细菌的分离及其促生长特性

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt1350144

Taiwo Vo, B. Vo, T. Vo, N. Nguyen

{"title":"Isolation of Endophytic Bacteria from Cavendish Banana pseudostem (Musa acuminata) and Plant Growth Promoting Characterization","authors":"Taiwo Vo, B. Vo, T. Vo, N. Nguyen","doi":"10.25303/1808rjbt1350144","DOIUrl":"https://doi.org/10.25303/1808rjbt1350144","url":null,"abstract":"Banana endophytic bacteria have many plant growth promoting activities and the enhanced influences based on the capable of endophytic colonization. Previous studies have mainly focused on endophytic bacteria in banana leaves, roots and shoots. This study aimed to evaluate the potential application of endophytic bacteria isolated from pseudostem of Cavendish banana. Eighteen endophytic bacterial isolates were collected from eight pseudostem samples. The tests showed that the indigenous endophytic bacteria, depending on the isolate, could dissolve insoluble inorganic minerals, produce extracellular hydrolytic enzymes, produce indole-3-acetic acid, fix nitrogen and control Phyllosticta sp. and Fusarium sp. Preliminary safety tests showed that one isolate did not exhibit hemolytic activity. Many isolates were compatible with each other, especially four strains including TC4.1, TC5.1, TC6.2 and TD7.1 were compatible with all remaining isolate and suitable for developing multi-strain products. Growth curves indicated that two isolates, TD7.2 and LA7.4, peaked at 16 and 18 hours respectively. The results showed that these endophytic bacteria have many potential activities. However, other plant growth promoting activities should be assessed in order to have potential applications as well as to exploit these bacteria.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44948156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of Brachyuran crabs after 12 years natural reforestation at destroyed areas by typhoon Durian in Can Gio mangrove 台风榴莲毁损区12年自然复林后赤枝蟹的多样性

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt039045

Tran Ngoc Diem My, Tran Le Quang Ha

{"title":"Diversity of Brachyuran crabs after 12 years natural reforestation at destroyed areas by typhoon Durian in Can Gio mangrove","authors":"Tran Ngoc Diem My, Tran Le Quang Ha","doi":"10.25303/1808rjbt039045","DOIUrl":"https://doi.org/10.25303/1808rjbt039045","url":null,"abstract":"The regeneration of mangroves in Can Gio following typhoon Durian has been the subject of interesting research, with the hope of developing plans to deal with similar consequences in the future. In this study, we recorded 26 species of crabs belonging to 16 genera and 10 families, of which the Sesarmidae and Ocypodidae families accounted for the highest proportion. The natural reforestation in the destroyed area by the previous Durian typhoon in 2006 has recorded the appearance of 6 new recorded species such as Paracleistostoma depressum, Pilumnopeus sp., Macrophthamus japonicus, Myomenippe hardwickii, Nanosesarma pontianacense and Uca annulipes. Particularly, Perisesarma eumolpe was the dominant species in all areas, indicating their ability to adapt to many habitats. Several new recorded species, especially Uca crabs, were recorded in naturally regenerated areas. The densities and biomass of crabs in regenerated areas were higher than those in intact forests. Shannon Weiner index indicated that the crab community had higher diversity in the regenerated areas. On the contrary, in the intact forest, the Simpson dominance index is higher than in the regenerated areas. The results of this study indicated that natural regeneration has created conditions for the crab community to recover opportunities for many new species to appear. The open canopy of monoculture forests would contribute to increasing the number of crabs,in particular and biodiversity, in general. Retaining the natural conditions after the typhoon had shown effectiveness in helping the ecosystem recover.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44646592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Blue and Red Light on the Hill Reaction of isolated Chloroplasts, Photosynthesis and Saponin Accumulation in Fenugreek Leaves (Trigonella foenum-graecum L.) 蓝光和红光对胡芦巴叶片叶绿体Hill反应、光合作用和皂苷积累的影响

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt074081

Huy Nguyen Tien Anh, Kiet Do Thuong, V. B. Trang

引用次数: 0

Tadpoles of Papurana milleti (Amphibia: Anura): molecular identification and morphological description 沙蛙蝌蚪(两栖纲:无尾目):分子鉴定和形态描述

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-07-15 DOI: 10.25303/1808rjbt068073

Thuy Duong Le Thi, Thinh Tran Gia, Tuyen Le Tran, Manh-Hung Pham

引用次数: 0