Microwave-assisted gelatin nanoparticles preparation: Green tea polyphenol mediated cross-linking and its enhanced bio-efficacy

Abstract

Gelatin nanoparticles (GNP) were successfully prepared by microwave-assisted method without using polar solvent acetone and cross-linker glutaraldehyde which are generally used in the double desolvation method. Moreover, we tried using green tea polyphenol (GTP) as a natural cross-linker. The particles were spherical, around 98.5 nm in size with a polydispersity index (P.I) of 0.813 and the surface charge was -11.6 mV. About 20% of free amino groups present in GNPs-GTP are made by microwave-assisted method with an encapsulation efficiency of 69.8 %. The predominant release was observed to fit in zero-order and Korsmeyer-Peppas kinetic models.