Reproductive biology最新文献_第9页

Mitophagy in mammalian follicle development and health 哺乳动物卵泡发育和健康中的丝裂吞噬作用

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-05-10 DOI: 10.1016/j.repbio.2024.100889

Zhengrong Zhou, Zhipeng Wu, Liufang Zhang, Yue Dai, Genbao Shao, Caifang Ren, Pan Huang

引用次数: 0

Identification of a novel mutation in chibby family member 2 in a non-obstructive azoospermic patient 在一名非梗阻性无精子症患者体内发现奇比家族成员 2 的新型基因突变

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-05-10 DOI: 10.1016/j.repbio.2024.100891

Guohui Zhang , Fei ye , Yihong Yang , Dongsheng xiong , Weiwei Zhi , Yang Wu , Yongkang Sun , Jiuzhi Zeng , Weixin Liu

{"title":"Identification of a novel mutation in chibby family member 2 in a non-obstructive azoospermic patient","authors":"Guohui Zhang , Fei ye , Yihong Yang , Dongsheng xiong , Weiwei Zhi , Yang Wu , Yongkang Sun , Jiuzhi Zeng , Weixin Liu","doi":"10.1016/j.repbio.2024.100891","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100891","url":null,"abstract":"<div><p>Azoospermia constitutes a significant factor in male infertility, defined by the absence of spermatozoa in the ejaculate, afflicting 15% of infertile men. However, a subset of azoospermic cases remains unattributed to known genetic variants. Prior investigations have identified the chibby family member 2 (<em>CBY2</em>) as prominently and specifically expressed in the testes of both humans and mice, implicating its potential involvement in spermatogenesis. In this study, we conducted whole exome sequencing (WES) on an infertile family to uncover novel genetic factors contributing to azoospermia. Our analysis revealed a homozygous c .355 C>A variant of <em>CBY2</em> in a non-obstructive azoospermic patient. This deleterious variant significantly diminished the protein expression of CBY2 both <em>in vivo and in vitro</em>, leading to a pronounced disruption of spermatogenesis at the early round spermatid stage post-meiosis. This disruption was characterized by a nearly complete loss of elongating and elongated spermatids. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and co-immunoprecipitation assays demonstrated the interaction between CBY2 and Piwi-like protein 1 (PIWIL1). Immunofluorescence staining further confirmed the co-localization of CBY2 and PIWIL1 in the testes during the spermatogenic process in both humans and mice. Additionally, diminished PIWIL1 expression was observed in the testicular tissue from the affected patient. Our findings suggest that the homozygous c .355 C>A variant of <em>CBY2</em> compromises CBY2 function, contributing to defective spermatogenesis at the round spermiogenic stage and implicating its role in the pathogenesis of azoospermia.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100891"},"PeriodicalIF":2.1,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human benign Leydig cell tumor − Biochemical evaluation 人类良性雷迪格细胞瘤 - 生化评估

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-05-08 DOI: 10.1016/j.repbio.2024.100890

Jan K. Wolski , Krzysztof Lustofin , Bartosz J. Płachno , Marta Zarzycka , Izabela Krakowska , Anna J. Korzekwa , Sylwia Zielinska , Beata Kusnierz-Cabala , Malgorzata Kotula-Balak

{"title":"Human benign Leydig cell tumor − Biochemical evaluation","authors":"Jan K. Wolski , Krzysztof Lustofin , Bartosz J. Płachno , Marta Zarzycka , Izabela Krakowska , Anna J. Korzekwa , Sylwia Zielinska , Beata Kusnierz-Cabala , Malgorzata Kotula-Balak","doi":"10.1016/j.repbio.2024.100890","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100890","url":null,"abstract":"<div><p>Recently we reported expressional alterations in 219 genes and their transcripts in Leydig cell tumors but nowadays there is still a lack of full basic biochemical characteristics of these tumors. The discovery of potential biochemical markers for tumor management from early detection, treatments, and control of therapy results may markedly supplement genetic data. Leydig cell micronodules were obtained from patients with azoospermia who were qualified for testicular biopsy. The biochemistry of Leydig cell tumors was analyzed using histological staining and spectrophotometric measurements of total proteins, carbohydrates, lipids, and nucleic acids. In addition, the levels of calcium (Ca2 +), copper (Cu2 +), zinc (Zn2 +), and selenium (Se2 +) ions were measured. When compared to healthy testis we revealed, for the first time, that in the interstitial tissue with Leydig cell tumors, great amounts of proteins, carbohydrates, lipids, and acids were dislocated from the seminiferous tubules. Measurements of organic compounds showed a decrease (<em>P</em> < 0.05) only in the Cu2 + content in Leydig cell tumors which may be related to their altered biochemical structure. This specific result may be promising for designing further approaches to manage this tumor based on combining morphological and molecular data.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100890"},"PeriodicalIF":2.1,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140880438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible Impact of Human β-defensin 1 on sperm motility in infertile men with abnormal sperm parameters 人类β防御素 1 对精子参数异常的不育男性精子活力的可能影响

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-04-29 DOI: 10.1016/j.repbio.2024.100887

Amir Masoud Firouzabadi , Mohammad Ebrahim Rezvani , Fateme Zare , Hossein Azizian , Farzaneh Fesahat

{"title":"Possible Impact of Human β-defensin 1 on sperm motility in infertile men with abnormal sperm parameters","authors":"Amir Masoud Firouzabadi , Mohammad Ebrahim Rezvani , Fateme Zare , Hossein Azizian , Farzaneh Fesahat","doi":"10.1016/j.repbio.2024.100887","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100887","url":null,"abstract":"<div><p>Human β-defensins and interleukins may be auxiliary in sperm maturation. This cross-sectional study aimed to evaluate the expression of <em>Human β-defensins 1</em> and <em>2</em>, interleukins (<em>ILs)− 10</em> and <em>-18</em> genes in sperm, as well as seminal plasma levels of these two cytokines in subfertile men with different types of sperm abnormalities compared to those with normozoospermic men. Participants were separated into two experimental groups: the control group (n = 25) and the group with sperm abnormalities (SA) (n = 45). SA participants were further subdivided into the following groups with n = 15 individuals each: Teratozoospermia (T), Asthenoteratozoospermia (AT), and Oligoasthenoteratozoospermia (OAT) groups. The quantitative real-time polymerase chain reaction was used to quantify the mRNA levels of <em>hBDs 1</em> and <em>2</em>, <em>IL-10</em>, and <em>IL-18</em> in sperm. The seminal plasma concentrations of IL-10 and IL-18 were measured by using the enzyme-linked immunosorbent assay technique. The mRNA expression of <em>hBD-1</em> and <em>IL-10</em> showed a significant decrease in the OAT compared to the controls (<strong>P < 0.0001</strong> and <strong>P = 0.02</strong>, respectively). The lowest seminal plasma concentration of IL-10 belonged to the OAT (<strong>P = 0.04</strong>). ROC curve analysis showed a sensitivity<em>,</em> specificity, and cutoff value of 82.35%, 86.67%, and 0.63 for <em>hBD-1</em> levels, respectively. A positive and significant correlation was found between <em>hBD-1</em> expression and sperm motility and <em>IL-10</em> expression rate and normal sperm morphology.Therefore, <em>hBD-1</em> could be considered as the alternative biomaterial to pre-treatments of infertile men with abnormal sperm parameters, specifically OAT men, which led to improving the assisted reproduction success rate.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100887"},"PeriodicalIF":2.1,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140807544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulation of Ethyl pyruvate on gestational diabetes mellitus in Mice; The impact on Th17/Treg balance 丙酮酸乙酯对小鼠妊娠糖尿病的免疫调节作用；对 Th17/Treg 平衡的影响

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-04-24 DOI: 10.1016/j.repbio.2024.100861

Min Zhao , Xiuping Bian , Li Meng

{"title":"Immunomodulation of Ethyl pyruvate on gestational diabetes mellitus in Mice; The impact on Th17/Treg balance","authors":"Min Zhao , Xiuping Bian , Li Meng","doi":"10.1016/j.repbio.2024.100861","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100861","url":null,"abstract":"<div><p>Gestational diabetes mellitus (GDM) is recognized as one of the most common diseases among pregnant women and inflammatory responses can be a major reason for its induction and development. T helper 17 (Th17)/regulatory T cells (Tregs) imbalance resulting in the increased levels of pro-inflammatory and decreased levels of anti-inflammatory cytokines has been showed as major mechanisms involved in the pathogenesis of GDM. There are various treatment options, but none of them are completely therapeutic. Ethyl pyruvate (EP) is a stable derivate of pyruvate that showed anti-oxidant and anti-inflammatory properties in an in-vivo and in-vitro models. To examine the therapeutic efficacy of EP in GDM, mice were mated and EP (100 mg/kg) was administered intraperitoneally to C57BL/6 mice. EP-treated mice exhibited improved symptoms of GDM by decreased blood glucose levels and body-weight and increased insulin levels and insulin sensitivity. Furthermore, EP could significantly attenuate the impairments to offspring, including birth size and birth weight. The inflammatory responses were also decreased by EP through regulating the production of Th17-related cytokines, such as interleukin (IL)− 17 and IL-21. The levels of other inflammatory cytokines were also inhibited, including IL-1β, IL-6, and tumor necrosis factor (TNF)-α. In addition, it was found that EP increased the population of Tregs and Treg-related cytokines, IL-10 and transforming Growth Factor-β TGF-β, in GDM mice. In conclusion, EP could modulate GDM in mice and might be a potential therapeutic strategy candidate for the treatment of patients with GDM.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100861"},"PeriodicalIF":2.1,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of MEK1/2 inhibitor U0126 on FGF10-enhanced buffalo oocyte maturation in vitro MEK1/2 抑制剂 U0126 对 FGF10 增强型水牛卵母细胞体外成熟的影响

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-04-20 DOI: 10.1016/j.repbio.2024.100883

Shanshan Du , Yanxin Wang , Fenghua Lu , Wenting Zhou

{"title":"Effects of MEK1/2 inhibitor U0126 on FGF10-enhanced buffalo oocyte maturation in vitro","authors":"Shanshan Du , Yanxin Wang , Fenghua Lu , Wenting Zhou","doi":"10.1016/j.repbio.2024.100883","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100883","url":null,"abstract":"<div><p>Fibroblast growth factor 10 (FGF10) plays critical roles in oocyte maturation and embryonic development; however, the specific pathway by which FGF10 promotes in vitro maturation of buffalo oocytes remains elusive. The present study was aimed at investigating the mechanism underlying effects of the FGF10-mediated extracellular regulated protein kinases (ERK) pathway on oocyte maturation and embryonic development in vitro. MEK1/2 (mitogen-activated protein kinase kinase) inhibitor U0126, alone or in combination with FGF10, was added to the maturation culture medium during maturation of the cumulus oocyte complex. Morphological observations, orcein staining, apoptosis detection, and quantitative real-time PCR were performed to evaluate oocyte maturation, embryonic development, and gene expression. U0126 affected oocyte maturation and embryonic development in vitro by substantially reducing the nuclear maturation of oocytes and expansion of the cumulus while increasing the apoptosis of cumulus cells. However, it did not have a considerable effect on glucose metabolism. These findings suggest that blocking the MEK/ERK pathway is detrimental to the maturation and embryonic development potential of buffalo oocytes. Overall, FGF10 may regulate the nuclear maturation of oocytes and cumulus cell expansion and apoptosis but not glucose metabolism through the MEK/ERK pathway. Our findings indicate that FGF10 regulates resumption of meiosis and expansion and survival of cumulus cells via MEK/ERK signaling during in vitro maturation of buffalo cumulus oocyte complexes. Elucidation of the mechanism of action of FGF10 and insights into oocyte maturation should advance buffalo breeding. Further studies should examine whether enhancement of MEK/ERK signaling improves embryonic development in buffalo.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100883"},"PeriodicalIF":2.1,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140622317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The consequences of manipulating relaxin family peptide receptor 1 (RXFP1) level in ovarian cancer cells 操纵卵巢癌细胞中松弛素家族肽受体 1 (RXFP1) 水平的后果

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-04-19 DOI: 10.1016/j.repbio.2024.100864

Kamila Domińska , Kinga Anna Urbanek , Karolina Kowalska , Dominika Ewa Habrowska-Górczyńska , Marta Justyna Kozieł , Tomasz Ochędalski , Agnieszka Wanda Piastowska-Ciesielska

{"title":"The consequences of manipulating relaxin family peptide receptor 1 (RXFP1) level in ovarian cancer cells","authors":"Kamila Domińska , Kinga Anna Urbanek , Karolina Kowalska , Dominika Ewa Habrowska-Górczyńska , Marta Justyna Kozieł , Tomasz Ochędalski , Agnieszka Wanda Piastowska-Ciesielska","doi":"10.1016/j.repbio.2024.100864","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100864","url":null,"abstract":"<div><p>Deregulation of the relaxin family peptide system (RFPS) appears to increase the risk of range of cancers, including epithelial ovarian cancers (EOC). The present study examines the effect of relaxin family peptide receptor 1 (RXFP1) level on the biological properties of human epithelial ovarian adenocarcinoma cells (OVCAR4 and SKOV3). RXFP1 was downregulated (RXFP1↓) in the cells using the RXFP1 sgRNA CRISPR All-in-One Lentivirus set (pLenti-U6-sgRNA-SFFV-Cas9–2A-Puro), and upregulated (RXFP1↑) using the RXFP1 CRISPRa sgRNA Lentivector (pLenti-U6-sgRNA-PGK-Neo) kit, which activates the RXFP1 gene when paired with dCas9-SAM. The changes taking place during adhesion to extracellular matrix (ECM) proteins were assessed in multi-well plates coated with collagen, fibronectin, laminin and gelatin. Cellular viability was monitored based on mitochondrial metabolic activity (MTT Assay, Alamar Blue Assay) and adenosine triphosphate production (ATP Assay). The rate of cell proliferation was determined based on the percentage of Ki67 immunoreactive cells and the numbers of cells in particular cell-cycle phases. The mesenchymal-like (Boyden Chamber Assay) and amoeboid-like movements (Wound Healing Assay) of ovarian cancer cells were also analyzed after transfection. RXFP1 downregulation decreased the adhesion properties of ovarian cancer cells and increased the tendency for apoptosis under stressful conditions. In contrast, RXFP1 upregulation had pro-proliferative, pro-survival and promigratory effects. Our findings confirm that the relaxin-2/RXFP1 signaling pathway plays a role in the promotion of growth and progression of ovarian cancer.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100864"},"PeriodicalIF":2.1,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140618727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of estradiol supplementation on endometrial thickness and intrauterine insemination outcomes 补充雌二醇对子宫内膜厚度和宫腔内人工授精结果的影响

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-04-17 DOI: 10.1016/j.repbio.2024.100886

Wendy Y. Zhang , Megan McCracken , Lisandra Veliz Dominguez , Amy Zhang , Jasmyn Johal , Lusine Aghajanova

{"title":"The impact of estradiol supplementation on endometrial thickness and intrauterine insemination outcomes","authors":"Wendy Y. Zhang , Megan McCracken , Lisandra Veliz Dominguez , Amy Zhang , Jasmyn Johal , Lusine Aghajanova","doi":"10.1016/j.repbio.2024.100886","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100886","url":null,"abstract":"<div><p>The impact of estrogen supplementation during the follicular/proliferative phase on the endometrial lining thickness (EMT) prior to intrauterine insemination (IUI) remains largely unstudied. Our study examined changes in EMT and rates of clinical pregnancy, miscarriage, and live birth for all patients who completed an IUI cycle at Stanford Fertility Center from 2017–2023 (n = 2281 cycles). Cycles with estradiol supplementation (n = 309) were compared to reference cycles without supplementation (n = 1972), with the reference cohort further categorized into cycles with a pre-ovulatory EMT of < 7 mm (“thin-lining”, n = 536) and ≥ 7 mm (“normal-lining”, n = 1436). The estradiol group had a statistically significant greater change in EMT from baseline to ovulation compared to the thin-lining reference groups (2.4 mm vs 1.9 mm, p < =0.0001). Similar rates of clinical pregnancy and live birth were observed. After adjusting for age, BMI, race/ethnicity, infertility diagnosis, and EMT at trigger, the estradiol cohort had a significantly increased odds of miscarriage versus the entire reference cohort (2.46, 95 % confidence interval [1.18, 5.14], p = 0.02). Thus, although estradiol supplementation had a statistically significant increase in EMT compared to IUI cycles with thin pre-ovulatory EMT (<7 mm), this change did not translate into improved IUI outcomes such as increased rates of clinical pregnancy and live birth or decreased rate of miscarriage. Our study suggests that supplemental estradiol does not appear to improve IUI outcomes.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100886"},"PeriodicalIF":2.1,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140558219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intrauterine infusion of platelet‐rich plasma improves fibrosis by transforming growth factor beta 1/Smad pathway in a rat intrauterine adhesion model 在大鼠宫内粘连模型中，宫腔内输注富血小板血浆可通过转化生长因子β1/Smad途径改善纤维化状况

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-04-10 DOI: 10.1016/j.repbio.2024.100882

Hongwen Yang , Wenmin Zhang , Jingchuan Fang , Zhihan Tang , Yanni Zhou , Hongzhen Hu , Miao miao , Yu Yao

{"title":"Intrauterine infusion of platelet‐rich plasma improves fibrosis by transforming growth factor beta 1/Smad pathway in a rat intrauterine adhesion model","authors":"Hongwen Yang , Wenmin Zhang , Jingchuan Fang , Zhihan Tang , Yanni Zhou , Hongzhen Hu , Miao miao , Yu Yao","doi":"10.1016/j.repbio.2024.100882","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100882","url":null,"abstract":"<div><p>This study aims to elucidate the effects of Platelet-rich plasma (PRP) in fibrosis development in intrauterine adhesion (IUA), and the associated underlying mechanisms are also explored, which are expected to be a potential therapeutic scheme for IUA. In this research, PRP was obtained and prepared from the peripheral venous blood of rats. A rat model was induced by mechanical injury. Further, PRP was directly injected into the uterus for treatment. The appearance and shape of the uterus were assessed based on the tissues harvested. The fibrosis biomarker levels were analyzed. The transforming growth factor beta 1 (TGF-β1) and Mothers against decapentaplegic homolog 7 (Smad7) levels, the phosphorylation of Smad2 (p-Smad2), and the phosphorylation of Smad3 (p-Smad3) were analyzed, and the molecular mechanism was investigated by rescue experiments. It was found that PRP improved the appearance and shape of the uterus in IUA and increased endometrial thickness and gland numbers. The administration of PRP resulted in a decrease in the expressions of fibrosis markers including collagen I, α-SMA, and fibronectin. Furthermore, PRP increased Smad7 levels and decreased TGF-β1 levels, p-Smad2, and p-Smad3. Meanwhile, administration of TGF-β1 activator reversed the therapeutic effects of PRP in IUA. Collectively, the intrauterine infusion of PRP can promote endometrial damage recovery and improve endometrial fibrosis via the TGF-β1/Smad pathway. Hence, PRP can be a potential therapeutic strategy for IUA.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100882"},"PeriodicalIF":2.1,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1642431X24000287/pdfft?md5=a855f417fe71be0e1273013b9a379103&pid=1-s2.0-S1642431X24000287-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140542845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of female sex hormones on proactive behavioral and physiological immune parameters 女性性激素对主动行为和生理免疫参数的影响

IF 2.1 3区生物学

Reproductive biology Pub Date : 2024-04-05 DOI: 10.1016/j.repbio.2024.100880

Judith K. Keller, Esther K. Diekhof

{"title":"Influence of female sex hormones on proactive behavioral and physiological immune parameters","authors":"Judith K. Keller, Esther K. Diekhof","doi":"10.1016/j.repbio.2024.100880","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100880","url":null,"abstract":"<div><p>Women may be more susceptible to infections in the luteal phase, supposedly as a consequence of the hormone progesterone and its immunosuppressive action. While immunosuppression may be important for successful oocyte implantation and pregnancy, it makes women more vulnerable to pathogens. According to theory, to compensate for reduced immunocompetence, women in the luteal phase exhibit proactive behavioral responses, such as disgust and avoidance of disease-associated stimuli, to minimize contagion risk. However, previous studies yielded inconsistent results, and did not account for accompanying proactive immune responses, like the increase of secretory immunoglobin A (sIgA). Here, we assessed the proactive immune response and feelings of disgust associated with disease cues in the comparison of 61 woman with a natural menstrual cycle (31 in the follicular and 30 in the luteal phase) and 20 women taking hormonal contraception (HC). Women rated disease vulnerability and disgust propensity, watched a video displaying people with respiratory symptoms, which was evaluated for its disgust-evoking potential and contagiousness, and provided saliva samples for hormone and sIgA analysis. Women with HC reported a heightened vulnerability to disease compared to naturally cycling women, whereas both the feeling of disgust and the sIgA increase elicited by the disease video were similar across groups, regardless of progesterone. We found a u-shaped relationship between progesterone and baseline sIgA in naturally cycling women, with its nadir during ovulation. Overall, our data do not support a compensatory relationship between the proposed progesterone-induced immunosuppression and heightened disgust or a proactive sIgA response.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100880"},"PeriodicalIF":2.1,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1642431X24000263/pdfft?md5=134ad095f5fea02fb0ddbb93284ea7c2&pid=1-s2.0-S1642431X24000263-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140348068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0