The alteration in myometrial mRNA transcription of the regulatory genes of DNA methylation in mare with endometrosis

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Beatriz Celeiro e Silva , Ewa Monika Drzewiecka , Katarzyna Piotrowska-Tomala , Joana Alpoim-Moreira , Agnieszka Sadowska , Magdalena Karolina Kowalik , Jorge Pimenta , Maria Rosa Rebordão , Graça Ferreira-Dias , Dariusz Skarzynski , Anna Szóstek-Mioduchowska
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Abstract

A reduction in myometrial contractile activity can lead to inadequate cleaning of the uterine lumen, resulting in persistent endometritis and potentially endometrosis in mares. Oxytocin (OXT) is a key hormonal regulator of myometrial contraction. While epigenetic regulation of myometrial gene expression has been studied in humans, there is limited information on the expression of DNA methyltransferases (DNMTs) and ten-eleven translocation enzymes (TETs) in the myometrium of mares. This study aimed to evaluate the mRNA transcription of these enzymes and the potential role of DNA methylation in the expression of the OXT receptor (OXTR) gene in the myometrium of mares with endometrosis. Myometrial samples were collected post-mortem during the mid-luteal (n = 23) and follicular (n = 20) phases of the estrous cycle and assessed according to Kenney and Doig endometrial category (I, IIA, IIB, III). mRNA transcription of OXTR, DNMT1, -3A, -3B and TET1, -2, -3 were determined using qPCR. DNA methylation analysis at CpG islands of OXTR exons 1 and 2 was performed using bisulfite pyrosequencing. Myometrial OXTR mRNA transcription and DNA methylation in its promoter region showed no significant differences between categories, although increased methylation was observed at CpG island position 6 in exon 2. DNMT1, TET2, and TET3 mRNA transcription was altered in the equine myometrium depending on the phase of the estrous cycle and the severity of endometrosis (P < 0.05). These findings indicate that DNMTs and TETs were expressed in myometrium in a manner specific to the severity of endometrosis and phases of the estrous cycle, suggesting a potential regulatory role in DNA methylation of myometrial gene expression.
子宫内膜异位症母马子宫肌层 mRNA 转录 DNA 甲基化调控基因的改变。
子宫肌收缩活动减弱会导致子宫腔清洁不足,造成持续性子宫内膜炎,甚至可能导致母马子宫内膜坏死。催产素(OXT)是子宫肌收缩的关键激素调节因子。虽然对人类子宫肌层基因表达的表观遗传调控已有研究,但有关母马子宫肌层 DNA 甲基转移酶(DNMTs)和十-十一转位酶(TETs)表达的信息却很有限。本研究旨在评估这些酶的 mRNA 转录情况,以及 DNA 甲基化在子宫内膜异位症母马子宫肌层 OXT 受体(OXTR)基因表达中的潜在作用。在发情周期的黄体中期(n = 23)和卵泡期(n = 20)收集子宫肌层样本,并根据 Kenney 和 Doig 的子宫内膜类别(I、IIA、IIB、III)进行评估,使用 qPCR 测定 OXTR、DNMT1、-3A、-3B 和 TET1、-2、-3 的 mRNA 转录。使用亚硫酸氢盐热测序法对 OXTR 外显子 1 和 2 的 CpG 岛进行了 DNA 甲基化分析。子宫肌层 OXTR mRNA 的转录及其启动子区域的 DNA 甲基化在不同类别之间没有明显差异,但在外显子 2 的 CpG 岛第 6 位观察到甲基化增加。马子宫肌层中 DNMT1、TET2 和 TET3 mRNA 的转录随发情周期的阶段和子宫内膜坏死的严重程度而改变(P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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