Reproductive biology最新文献

{"title":"Duration of gonadotropin support influences follicle growth and oocyte developmental competence in prepubertal calves","authors":"Ana Rita Tavares Krause ,&nbsp;Fernanda Caminha Faustino Dias ,&nbsp;Gregg Patrick Adams ,&nbsp;Reuben John Mapletoft ,&nbsp;Jaswant Singh","doi":"10.1016/j.repbio.2025.100996","DOIUrl":"10.1016/j.repbio.2025.100996","url":null,"abstract":"<div><div>The effect of duration of FSH support on follicle growth and oocyte developmental competence in prepubertal calves was investigated. Experiment 1 tested the effects of duration of FSH support (4 vs 7 days) and method of oocyte maturation (in vitro vs. in vivo) and provided a comparison with sexually mature heifers. Despite the compromising effect of unexpected ovulations in some prepubertal calves, embryo development from oocytes collected following 4 days of FSH support and in vitro maturation was similar to that from sexually mature heifers after 7 days of FSH support and in vivo maturation. In Experiment 2, prepubertal calves were given 4, 6 or 7 days of FSH support and oocytes were matured in vitro. At the time of oocyte collection, the number of follicles ≥ 6 mm was greater (<em>P</em> = 0.03) in calves given 7 days of FSH treatment than 4 days (37.3 ± 5.5 vs. 14.7 ± 2.5), but the number of cumulus-oocyte complexes collected did not differ among groups (<em>P</em> = 0.1). Oocytes from prepubertal calves given 6 days of FSH treatment had higher rates of cleavage and blastocyst formation than 4 or 7 days of treatment (cleavage = 73.2 vs. 51.3 vs. 47.2 % respectively, <em>P</em> = 0.01; blastocyst = 40.9 vs. 20.5 vs. 20.2 % respectively, <em>P</em> = 0.02). In conclusion, six days of FSH support under controlled endogenous LH release by exogenous progesterone in 5-month-old prepubertal calves was associated with greater developmental competence of oocytes than 4 or 7 days.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 100996"},"PeriodicalIF":2.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-harvest motility and morphological changes of spermatozoa following caudal epididymal recovery in farmed common eland (Tragelaphus oryx) bulls","authors":"Jerico Consolacion ,&nbsp;Francisco Ceacero ,&nbsp;Veit Ny ,&nbsp;Radim Kotrba ,&nbsp;Josef Illek ,&nbsp;Miša Škorič ,&nbsp;Marta Serralle ,&nbsp;Pedro Javier Soria-Meneses ,&nbsp;Ana Josefa Soler ,&nbsp;Tersia Kokošková","doi":"10.1016/j.repbio.2025.100997","DOIUrl":"10.1016/j.repbio.2025.100997","url":null,"abstract":"<div><div>The caudal epididymal recovery of spermatozoa has been utilised in game animal species to preserve genetically superior material after trophy hunting or slaughter, due to the difficulties of handling wild animals. Furthermore, the potential application of assisted reproductive techniques using harvested spermatozoa may contribute towards maintaining genetic diversity in isolated captive populations internationally. However, this technique requires clear and ideally simple protocols for the collection and handling of gametes after the death of the male animals, as the point of harvest is usually remote in game ranching. Therefore, this research aimed to investigate the effects of time after harvesting sperm under basic field conditions from the caudal epididymides of farmed common eland bulls in terms of their sperm motility and morphological changes. The relationship among these sperm quality parameters was also explored. Six bulls (2–2.5 years old; 203 ± 20 kg) were slaughtered, and their epididymal sperm were harvested and assessed for sperm motility and kinematics at minutes 0, 35, 70, 100, together with sperm viability, sperm head morphometry, and morphology at minutes 0, 20, 40, 60, 80 using CASA. Sperm quality sharply declined after 35 minutes of slaughter, but no effects were seen in sperm head morphometry. This study brings the first information regarding the quality of sperm retrieval from the cauda epididymides and sperm quality under physiological conditions in a uniform age group of eland. Future work should consider the effect of animal age and other individual animal traits, as well as the use of various media and extenders.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 100997"},"PeriodicalIF":2.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"E12.5 whole mouse embryo culture","authors":"Maxim A. Filatov,&nbsp;Leonid A. Ilchuk,&nbsp;Iuliia P. Baikova","doi":"10.1016/j.repbio.2025.100995","DOIUrl":"10.1016/j.repbio.2025.100995","url":null,"abstract":"<div><div><em>Ex utero</em> culture of postimplantation embryos remains one of the unsolved tasks in developmental biology. This technique may be required for infertility treatment, observation of embryo development and assessing the embryotoxicity of certain chemical agents. We describe novel method for E12.5 mouse embryos whole embryo culture system, which maintains embryo viability for 24 h. The culture system is based on the bubbling of pure oxygen through the culture medium. The oxygen was obtained by a chemical method. Each tube containing three embryos held 8 ml of culture medium composed of 6 ml of FBS, 2 ml of DMEM/F12, 80 μl of 40 % glucose and 30 μl of antibiotics (a mixture of penicillin 5000 UI/ml and streptomycin 5000 μg/ml). We observed initiation of auricle formation, as well as progression of eye development. Embryo viability was confirmed by the presence of heartbeat. The ratio of viable embryos after 24 h of culture was 27,78 %. However, many viable embryos exhibited massive hemorrhage attributed to oxygen insufficiency. The described culture system may be useful for the investigation of teratogenic compounds on the development of organs. Nonetheless, it is not suitable for <em>ex utero</em> culture of mouse embryos at more advanced stages due to the fact that embryos at such stages require more oxygen for the development than can be dissolved in the culture medium and consumed by embryos through diffusion. A potential solution to this issue is connecting the embryonic bloodstream to an oxygenator.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 100995"},"PeriodicalIF":2.5,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trophoblast-derived factors drive human mesenchymal stem cell differentiation along an endothelial lineage: A model of early placental vasculogenesis","authors":"Claire V. Harper ,&nbsp;Leah Eccles ,&nbsp;James Henstock ,&nbsp;Jayne C. Charnock","doi":"10.1016/j.repbio.2025.100994","DOIUrl":"10.1016/j.repbio.2025.100994","url":null,"abstract":"<div><div>Mechanisms controlling the process and patterning of blood vessel development in the placenta remain largely unknown. The close physical proximity of early blood vessels observed in the placenta and the cytotrophoblast, as well as the reported production of vasculogenic growth factors by the latter, suggests that signalling between these two niches may be important. Here, we have developed an <em>in vitro</em> model to address the hypothesis that the cytotrophoblast, by the secretion of soluble factors, drives differentiation of resident sub-trophoblastic mesenchymal stem cells (MSCs) along a vascular lineage, thereby establishing feto-placental circulation. BM-MSCs (a readily available model for placental stem cells) were treated with conditioned medium containing the secretome from human BeWo trophoblast cells, or endothelial growth medium (EGM2) supplemented with exogenous growth factors (VEGF, IGF1 and EGF) for 10–12 days. Trophoblast-conditioned media, found to contain detectable concentrations of cytokines including VEGF, uPAR, TIMP-1, TIMP-2, IL6 and placental growth factor, induced the expression of the endothelial genes <em>CD31</em>, <em>von Willibrand factor</em> (<em>vWF</em>), <em>FLT-1</em>, <em>VEGFR2</em> and <em>VE-Cadherin</em>. Upregulation of vWF protein was also detected following growth in trophoblast-conditioned media, using immunocytochemistry. Wound healing (migration assay) and Matrigel-tube formation assays confirmed that the BM-MSCs cultured in trophoblast-conditioned media exhibited functional measures of endothelial cells in addition to expressing relevant markers. Identification of key trophoblast-secreted factors and their promotion of endothelial differentiation in BM-MSCs helps advance our theories regarding the close relationship of the mesenchymal stem cell-cytotrophoblast niche in coordinating the complex angiogenic events that occur in the placenta. The <em>in vitro</em> model presented here provides an accessible and reproducible tool for further investigations into placental development.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100994"},"PeriodicalIF":2.5,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early and advanced glycation end product analysis from women with PCOS on metformin","authors":"Aditi S. Ambekar ,&nbsp;Nikita Naredi ,&nbsp;Dipankar Malakar ,&nbsp;Y. Vashum ,&nbsp;Pratibha Misra ,&nbsp;Mahesh Kulkarni","doi":"10.1016/j.repbio.2024.100993","DOIUrl":"10.1016/j.repbio.2024.100993","url":null,"abstract":"<div><div>In this cross-sectional study, we have analyzed advanced glycation end products (AGEs) in the plasma and follicular fluid of women with polycystic ovary syndrome (PCOS) taking metformin during <em>in vitro</em> fertilization (IVF) and control women undergoing IVF. Glucose, fructose, fructosamine, carboxymethyl lysine/ arginine (CML/R) proteins, and pentosidine were measured in the plasma and paired follicular fluid. Glycated proteins were characterized by mass spectrometry. Fasting serum glucose and fructosamine were comparable; however, follicular fluid glucose and fructosamine were higher in the PCOS group, and other AGEs remained unaltered. Fructose was lower in both serum and follicular fluid from the PCOS group. A positive correlation between some of these AGEs and sugars estimated was observed. Glucose and fructosamine in the follicular fluid correlated with the antral follicle count. The number of glycated peptides identified in the PCOS group by mass spectrometry was more. Glycated K75, K402 amino acid residues of albumin were detected in the PCOS group only. Additionally, some proteins involved in steroidogenesis and oocyte maturation as well as transporters, and extracellular matrix proteins, were found to be glycated in the PCOS group, which may affect their function. Elevated glucose and fructosamine in the follicular fluid of the PCOS group may contribute to abnormal folliculogenesis. The glycation of albumin should be validated in more samples to be considered as a marker for PCOS diagnosis.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100993"},"PeriodicalIF":2.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel screening approach for stem cell selective inhibitors and their possible translational therapeutic potential for endometriosis","authors":"Naoki Kimura ,&nbsp;Tomoka Takao ,&nbsp;Kazunori Imada ,&nbsp;Masanori Nakakuki ,&nbsp;Satoshi Kajikawa ,&nbsp;Tetsuo Maruyama","doi":"10.1016/j.repbio.2024.100992","DOIUrl":"10.1016/j.repbio.2024.100992","url":null,"abstract":"<div><div>Endometriosis is an estrogen-dependent benign disease characterized by growth of the endometrial tissue outside the uterine wall. Several reports suggest the possibility of the pathogenesis and recurrence of endometriosis being related to functions of stem/progenitor cells of the endometrium. The drawback of the widely used method of using Hoechst 33342, a fluorescent dye, to collect stem cell-like populations, is the requirement of an ultraviolet (UV) excitation source not commonly provided on standard flow cytometers. Here, we aimed to overcome this hurdle by establishing a novel method that uses DyeCycle Green (DCG), a cell-permeable DNA dye, for collecting a significantly higher fraction of stem cell-like side population (SP) from HHUA cells (human endometrial cancer cell line) with standard equipment without a UV laser. Furthermore, subculturing the DCG-SP cells expanded their population remarkably. The DCG-SP cells possessed stem cell-like characteristics with high expression of stem cell markers such as aldehyde dehydrogenase 1 A (ALDH1A), sushi domain containing 2 (SUSD2), increased colony formation ability, and high tumorigenicity in vivo, although the expression of some stem cell markers varied during expansion. We screened inhibitors for selective proliferation of the DCG-SP cells over immortalized endometrial cells (EM-E6/E7/hTERT-2 cells) and identified two effective compounds disulfiram and NSC319726. In addition, these compounds inhibited the colony formation and invasiveness of the DCG-SP cells. Our DCG-mediated screening of SP cells would possibly be translational to identify compounds that selectively target stem cells for the treatment and inhibition of recurrence of endometriosis.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100992"},"PeriodicalIF":2.5,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin and myometrial contractility; Are there any links? A narrative review","authors":"Pedram Ghafourifar ,&nbsp;Zahra Farahani ,&nbsp;Amir Hossein Norooznezhad ,&nbsp;Sedigheh Hantoushzadeh ,&nbsp;Mansour Azimzadeh ,&nbsp;Seyedeh Maedeh Nabavian ,&nbsp;Arezo Behzadian ,&nbsp;Quinn Kern Allely","doi":"10.1016/j.repbio.2024.100991","DOIUrl":"10.1016/j.repbio.2024.100991","url":null,"abstract":"<div><div>Contrary to the evidence supporting the role for insulin in stimulating uterine contraction, only a limited number of studies have highlighted the inhibitory effect of insulin on myometrial contractions in human and rodent. A hypothetical narrative review of the current literature was conducted, revealing the current literature and shows the potential inhibitory effects of insulin on myometrial contractility. These inhibitory mechanisms include activation of adenylyl cyclase signaling pathways, an increase in cAMP production, a decrease in Ca^2 + influx and cytosolic Ca²⁺, hyperpolarization of the cell membrane, and stimulation of NO synthesis. Altered oxytocin sensitivity, structural similarity to relaxin, modulating abscisic acid (ABA) effect, and synergistic interaction with progesterone, adiponectin, and leptin may also represent additional mechanisms for the inhibitory effects of insulin on myometrial contractions. The literature indicates that insulin exhibits inhibitory effects on myometrial contractility. Confirming such a conclusion through future studies may propose insulin as a possible uterine quiescent.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100991"},"PeriodicalIF":2.5,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclophosphamide-induced testicular injury is associated with inflammation, oxidative stress, and apoptosis in mice: Protective role of taxifolin","authors":"Afaf F. Almuqati","doi":"10.1016/j.repbio.2024.100990","DOIUrl":"10.1016/j.repbio.2024.100990","url":null,"abstract":"<div><div>Testicular damage is a major complication of chemotherapeutic cyclophosphamide (CP) compound. Taxifolin (TX), a natural flavonoid with well-established anti-inflammatory and antioxidant properties, is commonly found in various medicinal plants and foods. This study investigated the protective effect of TX against testicular damage in CP-administered mice. Mice were administered with TX at the doses of 10, 25, and 50 mg/kg for 15 days followed by a single CP injection on the 16th day. CP-administered mice demonstrated significantly decreased testosterone levels and low sperm parameters (count, viability, motility). TX administration significantly improved sperm parameters and testosterone levels and effectively mitigated histopathological testicular changes in CP-administered animals. Moreover, TX administration decreased oxidative stress markers and boosted antioxidants (superoxide dismutase, catalase, and reduced glutathione), suppressed and NF-κB p65 and pro-inflammatory cytokines [TNF-α (tumor necrosis factor-alpha) and IL-6 (interleukin-6)], and reduced apoptosis as depicted by testicular levels of caspase-3, Bcl-2, and Bax. Thus, TX could be a highly potent compound to counter CP-linked testicular damage through modulation of oxidative stress, inflammation, and apoptosis, warranting further studies to evaluate the role of TX in human CP-induced testicular injury.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100990"},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143098962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the endocrine-disrupting potential of atrazine for male reproduction: A systematic review and meta-analysis","authors":"Luiz Otávio Guimarães-Ervilha,&nbsp;Mírian Quintão Assis,&nbsp;Isabela Pereira da Silva Bento,&nbsp;Izabela da Silva Lopes,&nbsp;Thainá Iasbik-Lima,&nbsp;Renner Philipe Rodrigues Carvalho,&nbsp;Mariana Machado-Neves","doi":"10.1016/j.repbio.2024.100989","DOIUrl":"10.1016/j.repbio.2024.100989","url":null,"abstract":"<div><div>Atrazine is an herbicide widely used on plantations worldwide. Experimental studies suggest that the herbicide impairs male reproductive function in mammals. This systematic review and meta-analysis aimed to evaluate the impact of atrazine exposure on the levels of hormones from the hypothalamic-pituitary-testicular axis using murine as the animal model. After an extensive literature search, we selected 25 articles for the systematic review. Bias analysis and methodological quality assessments were examined using the SYRCLE Risk of Bias tool. Moreover, 20 out of the 25 studies were eligible for performing a meta-analysis to evaluate the intensity of atrazine damage on the levels of intratesticular testosterone and serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, estradiol, and progesterone. The meta-analysis revealed that atrazine exposure decreased serum FSH, LH, and testosterone levels, besides increased serum estradiol and progesterone levels. Atrazine also caused a reduction in intratesticular testosterone levels. Exposure to atrazine in high concentrations (≥ 100 mg Kg⁻¹) was the main cause of endocrine disruption, regardless of the exposure time. None of the studies have tested doses relevant to human health risk. Oxidative stress and inflammation are involved in atrazine toxicity, impairing the gonadotropin release by the pituitary, disturbing steroidogenesis, and affecting the male hormone regulatory system. We may conclude that hormone disturbances lead to a failure in testicular steroidogenesis, with possible implications for male reproductive function. The registration number on the Prospero platform is CRD42024495626.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100989"},"PeriodicalIF":2.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin-induced RBMS3 expression enhances ferroptosis and suppresses ovarian cancer progression","authors":"Yue Zhao ,&nbsp;Yixiao Wang ,&nbsp;Xinyi Zhang,&nbsp;Shuqi Han,&nbsp;Bo Yang","doi":"10.1016/j.repbio.2024.100968","DOIUrl":"10.1016/j.repbio.2024.100968","url":null,"abstract":"<div><div>Metformin (Met), a widely used type II diabetes medication, has shown anti-cancer properties in various cancers. RBMS3 is a tumor suppressor implicated in several cancers, including ovarian cancer. Ferroptosis, a novel form of programmed cell death, is gaining attention in cancer research. This study explores whether metformin induces ferroptosis and inhibits ovarian cancer progression through the RBMS3 pathway. We used a CCK-8 assay to determine the optimal metformin concentration for ovarian cancer cells. Metformin’s effects were further evaluated using EdU assay and flow cytometry. To clarify its mechanism, we employed programmed cell death inhibitors and measured levels of MDA (Malondialdehyde), GSH (Glutathione), and Fe²⁺. Ferroptosis-related proteins and RBMS3 expression in ovarian cancer tissues and cells were assessed via RT-qPCR and Western blotting. A xenograft mouse model was used to observe metformin’s effects on tumor growth. Metformin inhibited the viability of ovarian cancer A2780 cells, promoted ferroptosis, increased MDA and Fe²⁺ levels, and reduced GSH. It upregulated ferroptosis-related genes while downregulating GPX4 and SLC7A11. Although RBMS3 was reduced in cancer cells, metformin increased its expression, and silencing RBMS3 reversed metformin’s effects. In vivo, metformin inhibited tumor growth, which was negated by RBMS3 silencing. Our findings suggest that metformin promotes ferroptosis and inhibits ovarian cancer progression by upregulating RBMS3, offering a promising direction for clinical application in ovarian cancer treatment.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100968"},"PeriodicalIF":2.5,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}