Reproductive biology最新文献

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Chronic stress disturbs neuroendocrine control of reproduction and fertility in male rats 慢性应激干扰雄性大鼠生殖和生育的神经内分泌控制
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-05-24 DOI: 10.1016/j.repbio.2025.101027
Habit Medina , Alejandra Flores , Lizbeth Juárez-Rojas , Fahiel Casillas , Mohammad Mehdi Ommati , Reza Heidari , Sara Vázquez , Denise Clavijo-Cornejo , Sheila Peña-Corona , Socorro Retana-Márquez
{"title":"Chronic stress disturbs neuroendocrine control of reproduction and fertility in male rats","authors":"Habit Medina ,&nbsp;Alejandra Flores ,&nbsp;Lizbeth Juárez-Rojas ,&nbsp;Fahiel Casillas ,&nbsp;Mohammad Mehdi Ommati ,&nbsp;Reza Heidari ,&nbsp;Sara Vázquez ,&nbsp;Denise Clavijo-Cornejo ,&nbsp;Sheila Peña-Corona ,&nbsp;Socorro Retana-Márquez","doi":"10.1016/j.repbio.2025.101027","DOIUrl":"10.1016/j.repbio.2025.101027","url":null,"abstract":"<div><div>Currently, stress is considered one of the risk factors for infertility in male humans, altering sperm function. Sperm production and maturation depends on the hypothalamic-pituitary-testis axis control. Therefore, the objective of the current study was to evaluate the effects of chronic stress on the neuroendocrine control of male reproduction, the oxidative status in the epididymis, and male fertility. Adult male rats were assigned to control or chronic stress groups. Chronically stressed males were exposed to cold-water immersion (CWI) for 50 consecutive days. After euthanasia, the hypothalamus was dissected for Kisspeptin (Kiss1) and Gonadotropin releasing hormone (GnRH) evaluation; serum luteinizing hormone (LH), testosterone (T), and corticosterone concentrations were determined. In the epididymis, reactive oxygen species (ROS), lipid peroxides, and content of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx4) were assessed. Sperm motility, viability, concentration, morphology and acrosomal reaction were assessed. Epididymal sperm were used for in-vitro fertilization with oocytes from intact female rats. Stressed males showed lower hypothalamic Kiss1 and GnRH content, lower LH and T concentration, together with higher serum corticosterone concentration. ROS production, and lipid peroxides increased in all epididymal regions, while SOD, CAT, and GPx4 content decreased after chronic stress; sperm quality was also lower. The percentage of fertilized oocytes decreased, and embryonic development was low, compared to controls. Together, these results show that chronic stress disrupts neuroendocrine control of male reproduction and generates oxidative stress in the epididymis. These effects disturb sperm quality, leading to low fertilizing potential and poor embryonic development.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 3","pages":"Article 101027"},"PeriodicalIF":2.5,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Are BPA effects on Connexins and key downstream microRNAs in bovine COCs correlated to miR-21 expression? BPA对牛COCs中连接蛋白和关键下游microrna的影响是否与miR-21表达相关?
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-05-20 DOI: 10.1016/j.repbio.2025.101026
Thalia M. Goncalves, Olivia L.M. Scandlan, Casi B. Watson, Ola S. Davis, Fiona K. McIlhargey, Reem Sabry, Laura A. Favetta
{"title":"Are BPA effects on Connexins and key downstream microRNAs in bovine COCs correlated to miR-21 expression?","authors":"Thalia M. Goncalves,&nbsp;Olivia L.M. Scandlan,&nbsp;Casi B. Watson,&nbsp;Ola S. Davis,&nbsp;Fiona K. McIlhargey,&nbsp;Reem Sabry,&nbsp;Laura A. Favetta","doi":"10.1016/j.repbio.2025.101026","DOIUrl":"10.1016/j.repbio.2025.101026","url":null,"abstract":"<div><div>Endocrine disrupting compounds such as bisphenol A (BPA) negatively affect oocyte maturation and embryo development, while promoting epigenetic changes in miRNAs (miRs), including that of miR-21. miR-21 is an integral miR involved in various developmental processes, such as oocyte maturation, granulosa cells function and embryo development. Connexin (Cx) gap junction proteins play an important role in the communication among granulosa cells and cumulus cells and are impacted by bisphenols and linked to specific miRs. We hypothesize that miR-21 downregulation and BPA exposure affect Cxs mRNA and downstream miRNAs expression. Oocyte maturation was unaffected by miR-21 downregulation, but was significantly decreased (p = 0.0059) following BPA exposure. Also, BPA significantly increased Cx 43 mRNA levels when miR-21 is knocked-down in cumulus cells (p = 0.0476). miR-378a, a target of Cx 43 and 37, increased due to BPA and combination treatment in denuded oocytes (p = 0.0349) and cumulus cells (p = 0.0177). miR-96, a Cx 26 target, displayed a significant decrease in all samples following BPA exposure, in a miR-21 independent manner (p &lt; 0.05). These data strengthen previous evidence of a strict relationship among BPA, miR-21 and especially Cx 43 and miR-378a. Further investigations are worthy in elucidating these interactions and their response to BPA exposure during early development.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 101026"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polyploidy in germ cell of brown bear Ursus arctos (Ursidae, Mammalia): A case report 熊科棕熊生殖细胞多倍体1例
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-05-20 DOI: 10.1016/j.repbio.2025.101024
Lianna Virabyan , Ruzanna Petrosyan , Irina Ayvazyan , Emma Rose Khachatrian , Yana Dombrovskaya , Varvara Solovyeva , Victor Spangenberg
{"title":"Polyploidy in germ cell of brown bear Ursus arctos (Ursidae, Mammalia): A case report","authors":"Lianna Virabyan ,&nbsp;Ruzanna Petrosyan ,&nbsp;Irina Ayvazyan ,&nbsp;Emma Rose Khachatrian ,&nbsp;Yana Dombrovskaya ,&nbsp;Varvara Solovyeva ,&nbsp;Victor Spangenberg","doi":"10.1016/j.repbio.2025.101024","DOIUrl":"10.1016/j.repbio.2025.101024","url":null,"abstract":"<div><div>In this work, we present the first detailed study of the normal meiotic karyotype of the brown bear based on the analysis of synaptonemal complexes. Microphotographs of the key stages of meiotic prophase I were obtained. The level of meiotic recombination, features of XY sex bivalent assembly during meiotic prophase I were analyzed. A refined SC karyotype ideogram was constructed, consisting of 37 pachytene bivalents: 36 autosomal bivalents and and XY sex bivalent. We report a rare finding in mammalian germinal cells - a polyploid spermatocyte after genome duplication in the brown bear <em>Ursus arctos</em>. Meiotic tetravalents typical of autotetraploidy, the synaptic configurations consisting of homologous chromosomes and their corresponding duplicated copies, were visualized in this unique nucleus.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 101024"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Obesity, ovarian GLUT4 expression, and reproductive dysfunction: Insights from Zucker fatty rat 肥胖、卵巢GLUT4表达和生殖功能障碍:来自Zucker肥胖大鼠的见解
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-05-19 DOI: 10.1016/j.repbio.2025.101025
María Concepción Puga y Colmenares León , Angélica Trujillo-Hernández , Francisco Castelán , Mayra Xochitemol-Nava
{"title":"Obesity, ovarian GLUT4 expression, and reproductive dysfunction: Insights from Zucker fatty rat","authors":"María Concepción Puga y Colmenares León ,&nbsp;Angélica Trujillo-Hernández ,&nbsp;Francisco Castelán ,&nbsp;Mayra Xochitemol-Nava","doi":"10.1016/j.repbio.2025.101025","DOIUrl":"10.1016/j.repbio.2025.101025","url":null,"abstract":"<div><div>The impact of obesity on female reproductive health is considerable; despite the well-established association between nutritional status, obesity, and reproductive health, the mechanisms through which obesity impacts the reproductive axis remain poorly understood. For this reason, this study aims to investigate the expression of protein glucose transporter 4 (GLUT4) in the ovary and their association with morphophysiological alterations in the ovaries and uterus in a genetic obesity model, the Zucker fatty (fa/fa) rat. Various parameters were evaluated, including zoometric and metabolic measurements, estrous cyclicity, ovulation, and follicular development. The ovaries and uterus were histologically analyzed using hematoxylin-eosin staining. Furthermore, ovarian GLUT4 protein levels were estimated by Western blotting and fluorescence microscopy to investigate its association with metabolic and reproductive dysfunctions observed in this model. Zucker fatty rats exhibited significant body weight and adiposity index increases, accompanied by elevated cholesterol, triglycerides, and insulin levels. Conversely, progesterone levels were found to be significantly reduced. Histological analysis of ovarian morphology revealed pre-cystic follicles, a diminished follicular population, and a reduced number of corpora lutea. Additionally, a decrease in ovarian GLUT4 protein expression was observed. The uterine analysis demonstrated a smaller overall area, with notable reductions in both endometrial and myometrial thickness and fewer uterine glands. These findings indicate that in Zucker fatty rats, decreased ovarian GLUT4 protein expression and impaired glucose homeostasis are strongly associated with significant morphological alterations in the ovaries and uterus.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 101025"},"PeriodicalIF":2.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selection and validation of reference genes for gene expression studies of decidualization based on RNA sequencing 基于RNA测序的脱个体化基因表达研究内参基因的选择与验证
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-04-28 DOI: 10.1016/j.repbio.2025.101023
Haoyu Yang , Cancan Wang , Wenxuan Li , Xin Su , Mengyuan Li , Qian Li , Xiang-Hong Xu , Liping Jin
{"title":"Selection and validation of reference genes for gene expression studies of decidualization based on RNA sequencing","authors":"Haoyu Yang ,&nbsp;Cancan Wang ,&nbsp;Wenxuan Li ,&nbsp;Xin Su ,&nbsp;Mengyuan Li ,&nbsp;Qian Li ,&nbsp;Xiang-Hong Xu ,&nbsp;Liping Jin","doi":"10.1016/j.repbio.2025.101023","DOIUrl":"10.1016/j.repbio.2025.101023","url":null,"abstract":"<div><div>Decidualization is a multistep and complex physiological process used to aid the development of an implanting embryo. To date, the potential genes regulating decidualization have not been elucidated. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is widely used in gene expression studies, with relative quantification being the predominant method due to its simplicity, cost-effectiveness, and lower sample requirements. This method determines gene expression levels by normalizing to reference genes. However, the selection of stable reference genes for studies on decidualization remains a challenge. Based on the RNA-seq dataset from human endometrial stromal cells (ESCs) and differentiated ESCs (DESCs), ten new candidate reference genes were identified. The expression of these ten candidates, along with the commonly used reference gene β-actin, was measured in ESCs, DESCs, and decidual stromal cells (DSCs) through RT<img>qPCR. Five algorithms were used to systematically identify suitable reference genes. The results indicated that <em>Staufen double-stranded RNA binding protein 1 (STAU1)</em> was most stable for induced decidualization in vitro, showing consistent expression in ESCs and DSCs. Using <em>STAU1</em> as the reference gene, the expression levels of <em>insulin like growth factor binding protein 1</em> and <em>prolactin</em> in DESCs were significantly higher than those in ESCs. <em>Stau1</em> was further validated with both natural pregnancy and artificially induced decidualization mouse models. Based on our bioinformatics analysis, we also propose that <em>kelch like family member 9</em> and <em>TSC complex subunit 1</em> may serve as additional reference genes. Our findings offer valuable insights for gene expression studies of endometrial decidualization.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 101023"},"PeriodicalIF":2.5,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chemical profiling of testicular parenchyma in rams using an exploration of echointensity bands and the novel r-Algo computer algorithm increasing precision and accuracy of ultrasound image analyses 利用超声强度带和新型r-Algo计算机算法对公羊睾丸实质进行化学分析,提高超声图像分析的精度和准确性
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-04-26 DOI: 10.1016/j.repbio.2025.101021
Idris Farid , Namra Hamid , Jakub Bartlewski , Sareena Singh , Ali Mirshahi , Pawel M. Bartlewski , Bahareh Ahmadi
{"title":"Chemical profiling of testicular parenchyma in rams using an exploration of echointensity bands and the novel r-Algo computer algorithm increasing precision and accuracy of ultrasound image analyses","authors":"Idris Farid ,&nbsp;Namra Hamid ,&nbsp;Jakub Bartlewski ,&nbsp;Sareena Singh ,&nbsp;Ali Mirshahi ,&nbsp;Pawel M. Bartlewski ,&nbsp;Bahareh Ahmadi","doi":"10.1016/j.repbio.2025.101021","DOIUrl":"10.1016/j.repbio.2025.101021","url":null,"abstract":"<div><div>The aim of this study was to utilize bitmap segmentation of ultrasound images to enhance the consistency and accuracy of determining the chemical composition of ram testicular parenchyma based on its first-order echotextural characteristics, including mean numerical pixel intensity (MPI), standard deviation of numerical pixel values (mean pixel heterogeneity-MPH), and pixel frequency distribution (mean pixel concentration-MPC). Testes (n = 10) were obtained post-mortem from ten different, sexually mature Karakul rams and scanned using an 8-MHz linear-array transducer in both longitudinal (Long) and transverse (Trans) planes. The crude fat, protein, and moisture contents of testicular tissue samples were measured using validated laboratory techniques. Digital ultrasonograms of the testicular parenchyma were normalized and converted to bitmap files using commercially available ImageProPlus® analytical software. Two segmentation approaches were compared to assess their ability to detect quantitative correlations between echotextural characteristics and proximate chemical composition of testicular tissue: echointensity (EI) banding and algorithmic segmentation of the bitmaps. Using 50- or 25-pixel intensity bands, only two Long EI bands and five Trans EI bands showed significant correlations between MPI, MPH, or MPC and testicular tissue constituents. The accuracy of predicting testicular composition using algorithmically identified pixel intensity clusters ranged from 85.41 ± 3.67 % (MPI-fat) to 99.69 ± 0.12 % (MPC-moisture) for Long images, and from 78.31 ± 5.24 % (MPI-fat) to 99.76 ± 0.06 % (MPH-moisture) for Trans images. These results suggest that computerized detection of specific pixel intensity ranges offers an optimal method for determining the correlations between first-order echotextural characteristics and the chemical composition of ram testes.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 101021"},"PeriodicalIF":2.5,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of vitrification conditions on genetic and functional competence of prepubertal mouse oocytes 玻璃化条件对青春期前小鼠卵母细胞遗传和功能能力的影响
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-04-24 DOI: 10.1016/j.repbio.2025.101022
Akshatha Daddangadi , Siyona Saruparia , Dhakshanya Predheepan , Shubhashree Uppangala , Shama Prasada Kabekkodu , G. Nadeem Khan , Guruprasad Kalthur , Riccardo Talevi , Satish Kumar Adiga
{"title":"Impact of vitrification conditions on genetic and functional competence of prepubertal mouse oocytes","authors":"Akshatha Daddangadi ,&nbsp;Siyona Saruparia ,&nbsp;Dhakshanya Predheepan ,&nbsp;Shubhashree Uppangala ,&nbsp;Shama Prasada Kabekkodu ,&nbsp;G. Nadeem Khan ,&nbsp;Guruprasad Kalthur ,&nbsp;Riccardo Talevi ,&nbsp;Satish Kumar Adiga","doi":"10.1016/j.repbio.2025.101022","DOIUrl":"10.1016/j.repbio.2025.101022","url":null,"abstract":"<div><div>Oocyte <em>in vitro</em> maturation (IVM) and vitrification are being considered as fertility preservation strategies for prepubertal cancer patients. Since prepubertal oocytes have differential sensitivity and response to vitrification compared to adult oocytes, there is a need to optimize the technique to improve the outcome. This study specifically looked into the effect of varying equilibration time and temperatures on the survival and functional competence of prepubertal mouse oocytes. Germinal vesicle (GV) stage and in vitro matured, metaphase II stage sibling oocytes retrieved from 2-week-old <em>Swiss albino</em> mice were equilibrated at 24 °C and 37 °C for 10 and 15 min during vitrification. GV vitrified-IVM (GVV) and GV IVM-vitrified (MIIV) oocytes that survived post-warming were assessed for mitochondrial potential, spindle integrity, spindle checkpoint transcripts, and DNA integrity. The GVV oocytes equilibrated at 37 °C for 15 min had a significantly lower maturation rate (P &lt; 0.01). Survival was reduced when MIIV oocytes were equilibrated at 37 °C, regardless of equilibration duration (P &lt; 0.05). The meiotic spindle and DNA integrity were affected at 37 °C/15 min equilibration (P &lt; 0.01). IVM prepubertal mouse oocytes are at higher risk of experiencing cryo-damage with 37 °C equilibration. Hence, fertility preservation protocols must be refined and individualized for prepubertal age to safeguard the genetic and functional integrity of such oocytes.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 101022"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-mullerian hormone in felids: A systematic review 田间抗苗勒管激素:系统综述
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-04-19 DOI: 10.1016/j.repbio.2025.101016
Camila Lapuente , Augusto Lantermino , Sol Arioni , Paula G. Blanco , Cristina Gobello
{"title":"Anti-mullerian hormone in felids: A systematic review","authors":"Camila Lapuente ,&nbsp;Augusto Lantermino ,&nbsp;Sol Arioni ,&nbsp;Paula G. Blanco ,&nbsp;Cristina Gobello","doi":"10.1016/j.repbio.2025.101016","DOIUrl":"10.1016/j.repbio.2025.101016","url":null,"abstract":"<div><div>Anti-Müllerian hormone (AMH) is a dimeric glycoprotein that belongs to the transforming growth factor-β (TGF-β) superfamily. This hormone that is produced by gonadal Sertoli cells in males and granulosa cells in females has been extensively studied in humans, rodents, and livestock species. Research on AMH in felids began in 2011 and given the increasing number of studies over recent years, an updated literature review is necessary to clarify and organize future research directions. The objective of this article was to conduct a systematic review of AMH in domestic and wild felids. From a literature search of international publications, 23 were selected for inclusion. AMH determinations were performed using commercial enzyme-linked immunosorbent assays (ELISA) and electrochemiluminescence immunoassays (EQLIA). In female felids, AMH concentrations decrease with age, along with follicular reserve diminution. AMH can also be used to diagnose granulosa cell ovarian tumors and cryptorchidism in females and males, respectively. This hormone serves as a marker for reproductive status and can reflect gonadal function in both genders. Furthermore, AMH may prove to be a valuable predictive tool for reproductive biotechnologies in both domestic and wild felids. Several aspects of this hormone still remain to be elucidated, including its variations throughout the estrous cycle and the effect of photoperiod. Finally, standardization of assays and the establishment of reference ranges for both domestic and wild animals are necessary for widespread clinical application and future research development.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 101016"},"PeriodicalIF":2.5,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tributyltin affects the growth of ovarian granulosa cells in polycystic ovary syndrome by upregulating YY1-mediated CDKN1C via the PI3K/AKT pathway 三丁基丁通过PI3K/AKT通路上调yy1介导的CDKN1C,影响多囊卵巢综合征卵巢颗粒细胞的生长
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-04-19 DOI: 10.1016/j.repbio.2025.101020
Shitao Dong, Youbin Liu, Zhimin Yang
{"title":"Tributyltin affects the growth of ovarian granulosa cells in polycystic ovary syndrome by upregulating YY1-mediated CDKN1C via the PI3K/AKT pathway","authors":"Shitao Dong,&nbsp;Youbin Liu,&nbsp;Zhimin Yang","doi":"10.1016/j.repbio.2025.101020","DOIUrl":"10.1016/j.repbio.2025.101020","url":null,"abstract":"<div><div>Polycystic ovary syndrome (PCOS) poses a significant threat to women's fertility and quality of life. Studies have found a close association between the environmental contaminant tributyltin (TBT) and the occurrence of PCOS. The main objective of this study was to investigate the specific mechanisms by which TBT adversely affects the growth of ovarian granulosa cells. Cell viability, cycle, proliferation, and apoptosis were measured by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT), 5-ethynyl-2’-deoxyuridine (EdU), and flow cytometry. Simultaneously, lactate dehydrogenase (LDH) leakage and Caspase-3 activity were measured by the corresponding kits. Besides, western blot was used to analyze the protein levels of cyclin-dependent kinase inhibitor 1 C (CDKN1C) and the transcription factor Yin Yang 1 (YY1). TBT severely impaired the viability, cell cycle, and proliferation capacity of granulosa cells, and induced their apoptosis. Silencing CDKN1C and YY1 alleviated the damage caused by TBT to the cells, but these repair effects were weakened by CDKN1C overexpressed. By inhibiting the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway, TBT upregulated the YY1-mediated CDNK1C, and further exacerbated the damage to granulosa cells. This study revealed the mechanism that TBT induced the loss of ovarian granulosa cells in PCOS patients by upregulating YY1-mediated CDKN1C expression, which provided new ideas and targets for the pathogenesis and treatment of PCOS.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":"Article 101020"},"PeriodicalIF":2.5,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of amphiregulin on equine endometrial fibroblasts: in vitro responses of fibroblast derived from non-fibrotic and fibrotic endometrium 安非他酮对马子宫内膜成纤维细胞的影响:非纤维化和纤维化子宫内膜成纤维细胞的体外反应
IF 2.5 3区 生物学
Reproductive biology Pub Date : 2025-04-14 DOI: 10.1016/j.repbio.2025.101018
Anna Wójtowicz, Agnieszka Sadowska, Katarzyna Piotrowska-Tomala, Anna Szóstek-Mioduchowska
{"title":"The effect of amphiregulin on equine endometrial fibroblasts: in vitro responses of fibroblast derived from non-fibrotic and fibrotic endometrium","authors":"Anna Wójtowicz,&nbsp;Agnieszka Sadowska,&nbsp;Katarzyna Piotrowska-Tomala,&nbsp;Anna Szóstek-Mioduchowska","doi":"10.1016/j.repbio.2025.101018","DOIUrl":"10.1016/j.repbio.2025.101018","url":null,"abstract":"<div><div>The role of AREG in the development of fibrosis in the progression of endometrosis in mare remains unknown. We aimed to determine the effects of AREG on fibroblast functional characteristics as well as the expression of extracellular matrix (ECM)-associated genes in fibroblast derived from non-fibrotic and fibrotic equine endometria. Our findings suggest that the mechanisms associated with ECM remodeling regulated by AREG in non-fibrotic fibroblasts may be dysregulated in the progression of fibrosis in endometrosis.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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