Reproductive biology最新文献_第2页

Temporal optimization of meiotic arrest for enhancing oocyte maturity during in vitro maturation of porcine median antral follicles 在猪中腔卵泡体外成熟过程中，减数分裂阻滞对提高卵母细胞成熟度的时间优化。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-12-06 DOI: 10.1016/j.repbio.2024.100987

Ha Rin Namkung , Su Bin Jung , So Yeon Nam , Ji Won Han , Beak Song , Eun Song Lee , Seung Tae Lee

{"title":"Temporal optimization of meiotic arrest for enhancing oocyte maturity during in vitro maturation of porcine median antral follicles","authors":"Ha Rin Namkung , Su Bin Jung , So Yeon Nam , Ji Won Han , Beak Song , Eun Song Lee , Seung Tae Lee","doi":"10.1016/j.repbio.2024.100987","DOIUrl":"10.1016/j.repbio.2024.100987","url":null,"abstract":"<div><div>During in vitro maturation (IVM), median antral follicles (MAFs) were mechanically aspirated from the porcine ovarian cortex, and this process causes an early disconnection of follicular somatic cells from oocytes within antral follicles before the formation of graafian follicles. Thus, nuclear maturation is accelerated ahead of the completion of cytoplasmic maturation. Dibutyryl-cAMP (dbcAMP), a well-known cAMP modulator, is used to inhibit the resumption of meiosis in immature oocytes. However, there is no information on the optimal timeframe for sustaining meiotic arrest to enhance oocyte maturity during IVM. To determine the optimal duration of meiotic arrest, immature cumulus–oocyte complexes (COCs) from MAFs were cultured with 1 mM dbcAMP for 0, 4, 8, 12, 16, or 22 h, followed by further IVM without dbcAMP for 44, 40, 36, 32, 28, or 22 h. Subsequently, nuclear maturation, cumulus cell expansion score, perivitelline space size, glutathione (GSH) and reactive oxygen species (ROS) levels, and preimplantation development of parthenogenetic and in vitro-fertilized embryos were assessed in oocytes from each group. The results showed that a 16-h treatment with 1 mM dbcAMP within the 44-h IVM process yielded the highest oocyte maturity. Accordingly, we established an advanced IVM protocol for producing oocytes with superior maturity from porcine MAFs by achieving nuclear maturation 36 h after initiating IVM, using a 16-h treatment with 1 mM dbcAMP within the 44-h IVM process.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100987"},"PeriodicalIF":2.5,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Soluble luteinizing hormone receptor in follicular fluid and the association with reproductive function during IVF and ICSI IVF和ICSI期间卵泡液中可溶性促黄体生成素受体与生殖功能的关系

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-12-05 DOI: 10.1016/j.repbio.2024.100988

Li Juel Mortensen , Mette Lorenzen , Christine Hjorth Andreassen , Ursula Bentin-Ley , Hans Krog , Anders Juul , Martin Blomberg Jensen

{"title":"Soluble luteinizing hormone receptor in follicular fluid and the association with reproductive function during IVF and ICSI","authors":"Li Juel Mortensen , Mette Lorenzen , Christine Hjorth Andreassen , Ursula Bentin-Ley , Hans Krog , Anders Juul , Martin Blomberg Jensen","doi":"10.1016/j.repbio.2024.100988","DOIUrl":"10.1016/j.repbio.2024.100988","url":null,"abstract":"<div><div>The need for assisted reproductive technology (ART) has increased worldwide, leaving a negative impact on both physical and emotional health of the individual as well as on society depending on child births, thus biomarkers that can increase the success of ART are warranted. The luteinizing hormone/choriogonadotropin receptor (LHCGR) is released into blood and follicular fluid, and the level of soluble LHCGR (sLHCGR) in serum has previously been suggested to predict chances of pregnancy and live birth rate after ART. We aimed to investigate whether sLHCGR originates from the ovary and if serum or follicular fluid sLHCGR can predict the likelihood of pregnancy or live birth. A total of 133 women referred to a fertility clinic were included in the study, in total 203 trials of ART were performed. sLHCGR was analyzed in 170 follicular fluid samples and 75 serum samples. Interestingly, serum levels of sLHCGR were higher than follicular fluid levels (0.51 vs. 0.31 pmol/mL, p = 0.020) and a positive correlation in the two compartments was identified (r<sup>2</sup> 0.770, p < 0.0001), suggesting an extragonadal origin of sLHCGR. Follicular levels of sLHCGR were associated with follicular SHBG and free testosterone. Follicular/serum-ratio of sLHCGR was inversely associated with both serum- and follicular AMH. However, no association was found between sLHCGR and antral follicle count, which questions a link with folliculogenesis. Neither serum nor follicular fluid sLHCGR levels could predict pregnancy or live births in this cohort. Further studies are needed to clarify the role of sLHCGR in the ovaries.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100988"},"PeriodicalIF":2.5,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What's new in reproductive biology? Report from the 10th Meeting of the Society for Biology of Reproduction (Towarzystwo Biologii Rozrodu, TBR) 生殖生物学有何新进展？第十届生殖生物学学会（Towarzystwo Biologii Rozrodu，TBR）会议报告。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-12-01 DOI: 10.1016/j.repbio.2024.100974

Maria M. Guzewska , Patrycja Kurowska

{"title":"What's new in reproductive biology? Report from the 10th Meeting of the Society for Biology of Reproduction (Towarzystwo Biologii Rozrodu, TBR)","authors":"Maria M. Guzewska , Patrycja Kurowska","doi":"10.1016/j.repbio.2024.100974","DOIUrl":"10.1016/j.repbio.2024.100974","url":null,"abstract":"<div><div>The 10th Meeting of the Society for Biology of Reproduction (Towarzystwo Biologii Rozrodu, TBR) was held on September 12th-14th, 2024 in Warsaw, Poland. It was a continuation of previous meetings since 1999, when the first Meeting was held in Mierki near Olsztyn. As in previous meetings, the conference brought together an outstanding group of nearly 140 researchers, all connected by their work in reproductive biology across various animal models. During the lecture and poster sessions, participants explored a wide range of topics, including gamete and early embryonic development, assisted reproduction and fertility preservation, embryo-maternal interactions and pregnancy processes, and the hormonal, neural, and immune regulation of reproduction. Additionally, the discussions covered the environmental impact on reproductive function, as well as the development and function of the gonads. Additionally, two workshop events were organized: ‘Milestones on the road to a fruitful scientific career” for young researchers with a PhD degree, and “How to live with research failures and not be discouraged” for PhD students, which gave young researchers an opportunity to exchange their experience in the scientific field and discuss possible ways of handling crises. The conference was accompanied by an online outreach event entitled ‘Biology of reproduction for everyone’ (‘Biologia rozrodu dla każdego’) consisting of two lectures dedicated to different aspects of reproductive biology for secondary and high school students.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100974"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presence of embryos increases uterine blood flow and affects endometrial gene expression in Holstein cows on day seven post-insemination 受精后第7天，胚胎的存在增加了子宫血流量，影响了荷斯坦奶牛子宫内膜基因的表达

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-30 DOI: 10.1016/j.repbio.2024.100986

Dragos Scarlet , Susanne E. Ulbrich , Michael Steufmehl , Änne Honnens , Heinrich Bollwein

{"title":"Presence of embryos increases uterine blood flow and affects endometrial gene expression in Holstein cows on day seven post-insemination","authors":"Dragos Scarlet , Susanne E. Ulbrich , Michael Steufmehl , Änne Honnens , Heinrich Bollwein","doi":"10.1016/j.repbio.2024.100986","DOIUrl":"10.1016/j.repbio.2024.100986","url":null,"abstract":"<div><div>Superovulation treatment leads to characteristic changes in uterine perfusion during the first three weeks of pregnancy in cattle, but the origin of these changes is unknown. We investigated embryo-induced effects on uterine blood flow volume (uBFV) and pulsatility index (uPI), as well as on steroid hormone concentrations during the first seven days after conception, together with endometrial gene expression at the time of embryo flushing, in Holstein Friesian cows. Animals (n = 8) underwent (a) artificial insemination (AI), (b) superovulation followed by artificial insemination (SOV-AI), and (c) superovulation followed by sham AI (SOV-control) in a Latin square design. Doppler ultrasound examinations and blood collections were performed 12 h before AI, as well as on days 1, 3, 5, and 7 post AI. Embryo flushing followed by endometrial biopsy collection was also performed on day 7 post AI. While uBFV was higher after SOV-AI compared to AI on days −0.5, 3, 5 and 7 post AI (<em>P</em> < 0.05), uPI was not affected (<em>P</em> > 0.05). Progesterone and total estrogen concentrations were increased following superovulation at all timepoints (<em>P</em> < 0.05) but were not affected by the presence of embryos (<em>P</em> > 0.05). Compared to AI treatment, SOV-AI was associated with increased <em>KDR</em> and decreased <em>NOS3</em> endometrial mRNA expression (both <em>P</em> < 0.05) on day 7 post AI. Taken together, our results demonstrate changes in uterine blood flow during the first seven days after AI, and in endometrial gene expression in response to the presence of embryos at this time.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100986"},"PeriodicalIF":2.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium triggers AMPK-mTOR pathway to modulate autophagy related to oxidative stress of sheep Leydig cells 硒触发 AMPK-mTOR 通路，调节与绵羊亮德细胞氧化应激有关的自噬作用

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-24 DOI: 10.1016/j.repbio.2024.100973

Liang Ma , Kexin Li , Yaru Guo , Jinyu Liu , Jianing Dong , Jun Li , Youshe Ren , Lei Shi

{"title":"Selenium triggers AMPK-mTOR pathway to modulate autophagy related to oxidative stress of sheep Leydig cells","authors":"Liang Ma , Kexin Li , Yaru Guo , Jinyu Liu , Jianing Dong , Jun Li , Youshe Ren , Lei Shi","doi":"10.1016/j.repbio.2024.100973","DOIUrl":"10.1016/j.repbio.2024.100973","url":null,"abstract":"<div><div>The objective of this study was to investigate the effect of oxidative stress induced by excessive Se on autophagy of sheep Leydig cells and its underlying mechanism. Leydig cells isolated from the testis of 8-month-old sheep were purified using a discontinuous Percoll density gradient. Cells were divided into four treatment groups (0, 2.0, 4.0 and 8.0 μmol/L of Se). After treatment with Se for 48 h, cell proliferation was detected by CCK-8 assay kit. The biochemical methods were used to evaluate the antioxidant status of Leydig cells. The mRNA transcript and protein abundance related to the AMPK-mTOR pathway and autophagy were detected by real-time PCR and western blot analysis.</div><div>The results showed that the Leydig cells treated with 8.0 μmol/L Se have the lowest cell viability. The greater ROS content and lower GSH-Px activity were also observed in the Se<sub>8.0</sub> group. The inclusion of 2.0 μmol/L Se in the medium did not affect the autophagy of Leydig cells. However, the relative abundance of ATG5 protein and LC3II/I ratio were elevated in the Se<sub>8.0</sub> group. Oxidative stress induced by excessive Se (8.0 μmol/L) dramatically improved the abundance of key proteins related to AMPK-mTOR pathway and led to an increase of phosphorylated AMPK, mTOR and ULK1. Compared with the Se<sub>8.0</sub> group, compound C could significantly inhibit the key molecules of AMPK-mTOR signaling pathway and mitigate the autophagy of Leydig cells induced by excessive Se. These results indicate that appropriate Se (2.0 μmol/L) can enhance the viability of sheep Leydig cells. Oxidative stress caused by Se excess can induce cell autophagy via activating AMPK-mTOR signaling pathway. The existed crosstalk between autophagy and apoptosis could decide the fate of Leydig cells. This process could play a decisive role in the maintenance of normal male fertility and spermatogenesis by affecting the number of Leydig cells in testis.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100973"},"PeriodicalIF":2.5,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Empagliflozin improves sperm quality in diabetic rats by lowering oxidative stress and reducing apoptosis-related genes expression Empagliflozin 可通过降低氧化应激和减少细胞凋亡相关基因的表达来改善糖尿病大鼠的精子质量。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-23 DOI: 10.1016/j.repbio.2024.100971

Mina Kiani, Malek Soleimani Mehranjani, Mohammad Ali Shariatzadeh

{"title":"Empagliflozin improves sperm quality in diabetic rats by lowering oxidative stress and reducing apoptosis-related genes expression","authors":"Mina Kiani, Malek Soleimani Mehranjani, Mohammad Ali Shariatzadeh","doi":"10.1016/j.repbio.2024.100971","DOIUrl":"10.1016/j.repbio.2024.100971","url":null,"abstract":"<div><div>Type 2 diabetes mellitus (T2DM) negatively impacts sperm health by increasing oxidative stress. Empagliflozin, a type of sodium-glucose cotransporter 2 (SGLT2) inhibitor, not only reduces blood glucose levels in T2DM but also has antioxidant properties. This study aimed to investigate the antioxidant effects of empagliflozin on sperm quality and spermatogenesis indices in rats with T2DM.Male rats were divided into three groups (n = 6): control, diabetes mellitus (DM, induced by streptozotocin + nicotinamide), and DM treated with empagliflozin (10 mg/kg, 56 days, via gavage). Sperm parameters including sperm count, viability, motility, normal morphology, DNA integrity, maturation, tail length, and daily sperm production were analyzed. Additionally, spermatogenesis indices, Bax/Bcl2 ratio and serum levels of superoxide dismutase (SOD), total antioxidant capacity (TAC), and malondialdehyde (MDA) were measured. In the DM group, there was a significant decrease in the mean sperm parameters, SOD, TAC, and spermatogenesis indices, along with a significant increase in the percentage of single-stranded DNA (AO<sup>+</sup>), immature sperm nuclei (AB<sup>+</sup>), MDA levels, and Bax/Bcl2 ratio, compared to the control group. In the DM + empagliflozin group, there was a significant increase in the mean sperm parameters, SOD levels (P˂0.001), and spermatogenesis indices. Additionally, there was a significant decrease in the mean AB+ (P˂0.01), AO<sup>+</sup>, MDA levels (P˂0.001), and Bax/Bcl2 ratio compared to the DM group. The results showed that empagliflozin can mitigate the adverse effects of diabetes and improve sperm quality and increase sperm production in diabetic rats.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100971"},"PeriodicalIF":2.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of tarantula cubensis extract on ischemia-reperfusion injury in rat ovaries: Immunohistochemical, biochemical and histopathological evaluations 狼蛛提取物对大鼠卵巢缺血再灌注损伤的保护作用：免疫组织化学、生物化学和组织病理学评估。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-21 DOI: 10.1016/j.repbio.2024.100970

Zahid Paksoy , Sevim Beyza Ozturk Sarikaya , Nihat Yumusak

{"title":"Protective effect of tarantula cubensis extract on ischemia-reperfusion injury in rat ovaries: Immunohistochemical, biochemical and histopathological evaluations","authors":"Zahid Paksoy , Sevim Beyza Ozturk Sarikaya , Nihat Yumusak","doi":"10.1016/j.repbio.2024.100970","DOIUrl":"10.1016/j.repbio.2024.100970","url":null,"abstract":"<div><div>Ischemia-reperfusion (IR) injury is a common pathology affecting the ovary. This study aimed to investigate the effect of Tarantula cubensis extract (TCE) on ovarian IR injury. Forty-two female rats were used and randomly divided into 7 groups: Control, Sham, IR, Ischemia+TCE+Reperfusion, TCE+IR, Ischemia+Ethanol+Reperfusion and Ethanol+IR. The control and sham groups did not receive any treatment. All other groups were subjected to 3 h of ischemia + 3 h of reperfusion. A single dose of TCE (0.2 ml Theranekron) was administered subcutaneously either before or after ischemia in the treatment groups. In the last 2 groups, ethanol was applied before or after ischemia. After reperfusion, blood samples were collected and analyzed for biochemical parameters. Additionally, the ovarian tissue was evaluated histopathologically and immunohistochemically. The IR group exhibited more marked hyperemia, hemorrhage, and necrosis. Both treatment groups showed histopathological improvement. Moreover, the recovery rate was greater in the animals given theranekron before ischemia. IR injury caused oxidative stress in the rats. However, Theranekron did not significantly reduce the MDA levels (p > 0.05). On the other hand, SOD levels were significantly increased in the treatment groups (p < 0.01). Compared to the control and sham groups, the IL-1β, IL-6 and TNF-α levels were very high in the IR group. Conversely, the immunoreactivity of these cytokines was further reduced in the animals given theranekron before ischemia. Our findings suggest that TCE may be beneficial in the treatment of ovarian IR damage, especially when Theranekron is given before ischemia.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100970"},"PeriodicalIF":2.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic psychological stress induces testicular oxidative stress affecting reproductive behavior in rats 慢性心理压力会诱发影响大鼠生殖行为的睾丸氧化应激。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-20 DOI: 10.1016/j.repbio.2024.100934

Tianfeng Yang , Xinye Liu , Chunyan Kang , Gonglin Hou , Yunyun Shen , Zheqi Liu

引用次数: 0

Unveiling Leydig cell heterogeneity and its role in male infertility: A single-cell transcriptomic study of human testicular tissue 揭示睾丸细胞异质性及其在男性不育症中的作用：人类睾丸组织的单细胞转录组研究。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-19 DOI: 10.1016/j.repbio.2024.100972

Liwei Zhou , Hanchao Liu , Yuming Chen , Lin Hua , Xiaolong Wu , Xintao Gao , Le Mao

{"title":"Unveiling Leydig cell heterogeneity and its role in male infertility: A single-cell transcriptomic study of human testicular tissue","authors":"Liwei Zhou , Hanchao Liu , Yuming Chen , Lin Hua , Xiaolong Wu , Xintao Gao , Le Mao","doi":"10.1016/j.repbio.2024.100972","DOIUrl":"10.1016/j.repbio.2024.100972","url":null,"abstract":"<div><div>Male infertility and impaired spermatogenesis are significant concerns in reproductive health, often linked to disruptions in the cellular and molecular processes within the testis. The cellular composition and transcriptional dynamics of human testicular tissue are crucial for understanding these issues. Previous studies have largely relied on bulk tissue analysis, which obscures the distinct roles and interactions of specific cell types. Here, through a comprehensive single-cell transcriptomic analysis of human testes across various developmental stages and pathological conditions, we reveal the intricate cellular heterogeneity and the molecular mechanisms underlying testicular function. Our study identifies significant disruptions in the differentiation trajectories of Germ cells in conditions such as Klinefelter syndrome (KS), AZFa deletion, and Sertoli-cell-only syndrome (SCOS). We further uncover key transcription factors and regulatory networks governing Leydig cell function, particularly those related to steroidogenesis and hormonal regulation. These findings highlight the organized yet complex cellular and molecular landscape of the testis and uncover critical pathways altered in male infertility. Collectively, our data suggest that targeted therapeutic strategies could be developed to address specific disruptions in testicular cell populations and their associated regulatory networks.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100972"},"PeriodicalIF":2.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the pathophysiology of polycystic ovary syndrome: Construction and analysis of a ceRNA network in cumulus cells 阐明多囊卵巢综合征的病理生理学：构建并分析积液细胞中的ceRNA网络

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-19 DOI: 10.1016/j.repbio.2024.100916

Jingjing Li , Li Fan , Jiajia Wei , Wenjie Huang

{"title":"Elucidating the pathophysiology of polycystic ovary syndrome: Construction and analysis of a ceRNA network in cumulus cells","authors":"Jingjing Li , Li Fan , Jiajia Wei , Wenjie Huang","doi":"10.1016/j.repbio.2024.100916","DOIUrl":"10.1016/j.repbio.2024.100916","url":null,"abstract":"<div><div>Polycystic Ovary Syndrome (PCOS) is a complex endocrine disorder with elusive molecular mechanisms. This study explores the competitive endogenous RNA (ceRNA) regulatory network in the cumulus cells of PCOS patients. ceRNAs are transcripts like mRNAs, miRNAs, and lncRNAs that competitively bind shared miRNAs, regulating gene expression post-transcriptionally. We analyzed mRNA, microRNA (miRNA), and long non-coding RNA (lncRNA) from two cohorts: 12 PCOS patients and 11 healthy controls (dataset GSE10946), and 5 PCOS patients and 5 healthy controls (dataset GSE72274). These microarray datasets, obtained from the Gene Expression Omnibus (GEO), helped us identify differentially expressed mRNAs, miRNAs, and lncRNAs. Our analysis revealed a significant ceRNA network, which may play a crucial role in the pathophysiology of PCOS. In this network, 5 lncRNAs, 3 miRNAs, and 36 mRNAs were identified as differentially expressed. These elements form a complex regulatory schema influencing key cellular processes related to the disease, such as cell cycle regulation and response to estrogen. The HOXA11-AS-hsa-miR-454–3p-CCND2 network emerged as a potentially valuable biomarker for PCOS diagnosis, supported by Receiver Operating Characteristic (ROC) curve analysis indicating strong predictive power. Our findings suggest that the ceRNA interactions in PCOS cumulus cells provide a deeper understanding of the disease's molecular basis and offer new avenues for therapeutic intervention. This in silico study lays the groundwork for further experimental validation of these ceRNA networks as targets for PCOS treatment.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100916"},"PeriodicalIF":2.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0