Reproductive biology最新文献

{"title":"Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity","authors":"Anabella Andrea Campagna ,&nbsp;Mariana Carolina Fabra ,&nbsp;Noelia Nikoloff ,&nbsp;Ana Cristina Carranza-Martin","doi":"10.1016/j.repbio.2025.101087","DOIUrl":"10.1016/j.repbio.2025.101087","url":null,"abstract":"<div><div>Fluazuron is a veterinary antiparasitic compound widely used as pour-on in cattle. However, little is known about its potential reproductive toxicity. This study aimed to evaluate the effects of fluazuron on sperm quality, bovine oviduct epithelial cells (BOECs), and bovine <em>in vitro</em> fertilization (IVF). Frozen-thawed sperm were exposed to fluazuron (50 µg/mL) for 2 h and evaluated for motility, mitochondrial activity, and membrane integrity, while BOECs were cultured with fluazuron for 24 h and assessed using MTT and comet assays. IVF was performed with gametes exposed to fluazuron for 18 h to evaluate pronuclear formation, and 24 h to study embryo development. Short-term fluazuron did not significantly affect sperm motility, viability, or membrane function. However, it decreased viability and induced DNA damage in BOECs after 24 h of treatment. Following fluazuron exposure during IVF, no differences were observed between the fluazuron-treated group and the control group in terms of pronuclear formation. However, a significantly higher incidence of abnormally condensed chromatin within the pronuclei was detected in the fluazuron-treated group. Furthermore, embryonic development decreased in both blastocyst and hatching rates in fluazuron treatment. These findings suggest that fluazuron may disrupt early reproductive events through multiple mechanisms, including BOEC dysfunction and chromatin damage. Our results highlight the importance of evaluating reproductive risks associated with antiparasitic drug exposure in livestock.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101087"},"PeriodicalIF":2.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145157835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent pregnancy loss: Crosstalk between immune cells, decidual cells, and cellular autophagy","authors":"Xingxing Yuan ,&nbsp;Chaofan Li ,&nbsp;Xiaoyu Zhang ,&nbsp;Xiaoling Feng","doi":"10.1016/j.repbio.2025.101083","DOIUrl":"10.1016/j.repbio.2025.101083","url":null,"abstract":"<div><div>Recurrent pregnancy loss (RPL), affecting ∼5 % of couples, remains idiopathic in up to 50 % of cases. This review synthesizes the multifactorial pathogenesis of RPL, emphasizing dysregulated molecular pathways, genetic polymorphisms such as NOS2 rs2779249, and environmental triggers such as <em>Toxoplasma gondii</em> infection and pesticide exposure. Immune dysfunction is central, characterized by altered Treg subsets such as reduced CD4⁺ and increased CD8⁺ Tregs, impaired checkpoint expression such as PD-1/PD-L1 and Tim-3, aberrant cytokine profiles such as elevated IL-7, IL-1β, TNF-α and reduced TGF-β, and decidual NK (dNK) cell dysregulation impacting trophoblast invasion via pathways like IGF-2/PEG10. Non-coding RNAs further contribute by promoting trophoblast apoptosis, suppressing migration/invasion, and driving inflammation through MAPK/JNK/NF-κB signaling. Decidualization defects involve metabolic imbalance, unresolved endoplasmic reticulum stress, microRNA dysregulation, TNFα-induced senescence, and disrupted β-catenin/STAT3 crosstalk. Autophagy exhibits dual role: while protective in decidual stromal cells (DSCs) by supporting Treg expansion and immune tolerance, its dysregulation triggers trophoblast apoptosis via MCL-1 degradation and caspase activation. Environmental toxins exacerbate oxidative stress and apoptosis. Therapeutic prospects include immune checkpoint agonists, lncRNA/miRNA antagonists, autophagy flux modulators, growth factor therapies, and LMWH targeting thrombotic and inflammatory pathways. However, clinical translation faces challenges, including pathway duality, pleiotropic effects, targeted delivery limitations, and limited human trial data. Future research should focus on single-cell resolution studies, personalized approaches, and safer nanocarriers for biologics to bridge mechanistic insights into effective RPL interventions.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101083"},"PeriodicalIF":2.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding ovarian aging in women: Cellular damage, signaling networks, and treatment frontiers","authors":"Shivani Ingole,&nbsp;Kanchan Khare,&nbsp;Veepin Dwivedi,&nbsp;Brijesh Taksande,&nbsp;Milind Umekar,&nbsp;Shubhada Mangrulkar","doi":"10.1016/j.repbio.2025.101075","DOIUrl":"10.1016/j.repbio.2025.101075","url":null,"abstract":"<div><div>Ovarian aging is a significant biological process characterized by the gradual decline of ovarian function and fertility in women as they age. It is a multifaceted process that involves various molecular mechanisms. This review article delves into the complex nature of ovarian aging, marked by reductions in both the quantity and quality of oocytes, hormonal imbalances, and heightened risks of infertility and pregnancy complications. It consolidates current understanding of the physiological, cellular, and molecular mechanisms driving ovarian aging, such as mitochondrial dysfunction, oxidative stress, telomere shortening, DNA (Deoxyribonucleic Acid) damage, inflammation, and apoptosis. This review primarily focuses on human ovarian aging, while also integrating relevant insights from animal models particularly rodent studies that have contributed to our understanding of underlying mechanisms. It explores key signaling pathways involved in aging, including AMPK (AMP-Activated Protein Kinase), mTOR (mammalian target of rapamycin), Nrf2 (Nuclear Factor Erythroid 2-Related Factor 2), SIRT1 (Sirtuin 1), and FOXO3 (Forkhead Box O Transcription Factor) pathways. The review also discusses emerging therapeutic strategies designed to delay or reverse ovarian aging, which include antioxidants, hormone replacement therapy, stem cell-based treatments, CRMs (CR mimetics), gene therapy, and traditional medicines. Additionally, the article examines the potential role of polyamines in ovarian function and aging. By thoroughly analyzing the current research landscape and identifying future research directions, this review offers valuable insights for researchers and clinicians dedicated to improving reproductive health and quality of life for aging women.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101075"},"PeriodicalIF":2.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadal morphogenesis and sexual differentiation in rhea (Rhea americana)","authors":"Marilú Cristofoli ,&nbsp;Flavia Maria Pia Montenegro Donoso ,&nbsp;Mayla Magalhães de Oliveira Alcobaça ,&nbsp;Fabiana Morse Gosson Jorge ,&nbsp;Danielle Cristina Calado de Brito ,&nbsp;Moacir Franco de Oliveira ,&nbsp;Antônio Chaves de Assis Neto","doi":"10.1016/j.repbio.2025.101079","DOIUrl":"10.1016/j.repbio.2025.101079","url":null,"abstract":"<div><div>The rhea (<em>Rhea americana</em>) is a docile and highly adaptable ratite with notable zootechnical potential for the production of meat, eggs, feathers, and leather. Beyond its economic relevance, captive breeding plays an important role in conservation. However, information on its embryonic and gonadal development remains limited—knowledge that is essential for improving reproductive management. This study aimed to characterize gonadal differentiation during artificial incubation, focusing on developmental stages at days 9, 12, 15, 18, 21, and 24. We provide a detailed morphological and cytological description of germ and somatic cells, documenting the appearance of the gonadal crest at day 9, the formation of undifferentiated gonads by day 12, and the onset of sexual differentiation at day 15. Morphometric analyses of cortex thickness and gonad–mesonephros area relationships enabled comparisons between sexes. The gonads were positioned cranioventrally on the mesoneph, and testicular maturation occurred earlier than ovarian differentiation. Transmission electron microscopy revealed greater mitochondrial density in male germ cells than in female germ cells, indicating early functional divergence. These results contribute to the understanding of reproductive development in <em>R. americana</em> and support the development of conservation and breeding strategies for this and related species.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101079"},"PeriodicalIF":2.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled ovarian hyperstimulation reduces mouse embryo implantation capacity through uterine HIF-2α mediated pathway","authors":"Zheng Zhi ,&nbsp;Haijun Yang ,&nbsp;Zhangming Liu ,&nbsp;Wei Pan ,&nbsp;Yanzhi Hao ,&nbsp;Xinming Zhang ,&nbsp;Shuancheng Zhang ,&nbsp;Huazhou Xu ,&nbsp;Huirong Ma ,&nbsp;Huilan Du ,&nbsp;Cuimiao Song ,&nbsp;Qiong Wu","doi":"10.1016/j.repbio.2025.101074","DOIUrl":"10.1016/j.repbio.2025.101074","url":null,"abstract":"<div><div>Controlled ovarian hyperstimulation (COH) is a cornerstone of assisted reproductive technologies, yet its effects on endometrial function and embryo implantation remain poorly understood, particularly regarding the role of hypoxia-inducible factor 2α (HIF-2α) signaling. Therefore, the objective of this study was to investigate whether COH-induced endometrial dysfunction impairs mouse embryo implantation through the HIF-2α pathway. A COH mouse model was established using gonadotropin-releasing hormone agonist (GnRH-a)/human menopausal gonadotropin (hMG)/human chorionic gonadotropin (hCG) administration. Embryo implantation status was evaluated on gestational days 5, 6, and 20; endometrial tissues were analyzed for HIF-2α pathway activity via immunohistochemistry (IHC), immunofluorescence (IF), Western blot (WB) and quantitative real-time PCR (qRT-PCR); histological changes were assessed by Jones silver staining and transmission electron microscopy (TEM); serum estradiol (E₂), progesterone (P₄), and prolactin (PRL) levels were measured by radioimmunoassay (RIA). COH mice exhibited reduced total embryo implantation rates (on day 5, 6 and 20), together with decreased serum E₂, P₄, and PRL levels. COH mice exhibited preserved luminal epithelium integrity with increased microvillus density and continuous basement membrane structure. qRT-PCR and WB demonstrated significantly downregulated HIF-2α expression at both mRNA and protein levels, accompanied by reduced downstream RAB27B (member of RAS oncogene family)/matrix metalloproteinase 9 (MMP9) and lysyl oxidase (LOX)/adrenomedullin (ADM) signaling, which associated with impaired luminal epithelium detachment and compromised trophoblast invasion. Together, these findings identify HIF-2α as a potential key mediator of COH-induced endometrial microenvironment alterations, revealing molecular mechanisms underlying implantation failure. Importantly, the HIF-2α-RAB27B/MMP9 and HIF-2α-LOX/ADM axes are highlighted as promising therapeutic targets to optimize assisted reproductive outcomes.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101074"},"PeriodicalIF":2.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing the toxicological effects of PET-MPs on male infertility: Insights from network toxicology, mendelian randomization, and transcriptomics","authors":"Yang Yang ,&nbsp;Linfeng Li ,&nbsp;Ning Zhang ,&nbsp;Zheng Zhang ,&nbsp;Zhengyang Wu ,&nbsp;Dongmei Ji","doi":"10.1016/j.repbio.2025.101076","DOIUrl":"10.1016/j.repbio.2025.101076","url":null,"abstract":"<div><div>Current research indicates that polyethylene terephthalate microplastics (PET-MPs) may significantly impair male reproductive function. This study aimed to investigate the potential molecular mechanisms underlying this impairment. Potential gene targets of PET-MPs were predicted via the SwissTargetPrediction database. GWAS summary statistics for male infertility were obtained from the GWAS Catalog, and eQTL data were acquired from the eQTLGen database. SMR analysis was subsequently performed to identify genes exhibiting significant causal associations with male infertility. The potential targets of PET-MPs contributing to male infertility were ultimately identified by intersecting the two gene sets. Additionally, bulk RNA-seq and scRNA-seq analyses were employed to elucidate the potential molecular mechanisms underlying PET-MPs-induced male infertility. Our findings suggest that <em>CLK4</em> may serve as a functional target through which PET-MPs contribute to male infertility, with elevated <em>CLK4</em> expression representing a significant risk factor. Molecular docking and molecular dynamics simulations demonstrated that PET-MPs can form stable binding conformations with the <em>CLK4</em> protein. We further identified terminally differentiated CD4⁺ T cells as a prominent risk factor for male infertility, revealing a significant positive correlation between <em>CLK4</em> expression levels and Th1 cell infiltration. Additionally, <em>CLK4</em> exhibited a biphasic expression pattern during spermatocyte-to-sperm differentiation, initially increasing before subsequent downregulation. These observations indicate that PET-MPs may participate in the pathogenesis of male infertility by targeting <em>CLK4</em> to modulate Th1 cell infiltration and disrupt normal spermatogenic processes. In conclusion, our study demonstrates that <em>CLK4</em> may serve as a potential target for PET-MP-induced male infertility, and we further elucidate the underlying molecular mechanisms.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101076"},"PeriodicalIF":2.5,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pasteurized Akkermansia muciniphila ameliorates insulin resistance by reducing placental inflammation in GDM mouse model","authors":"Yi Wang ,&nbsp;Yuting Zhu ,&nbsp;Yan Cui ,&nbsp;Jiali Fang ,&nbsp;Hong Zhong ,&nbsp;Yujie Shi ,&nbsp;Lan Liu ,&nbsp;Xianwei Cui","doi":"10.1016/j.repbio.2025.101073","DOIUrl":"10.1016/j.repbio.2025.101073","url":null,"abstract":"<div><div>Gestational diabetes mellitus (GDM) is a common and serious complication during pregnancy. Depleted next-generation probiotic, Akkermansia muciniphila (AKK) in GDM women indicates its potential on GDM prevention. However, the functions and mechanisms of AKK on GDM remain unclear. Due to the limited strategies for GDM therapy, combined with the anaerobic properties of AKK, herein, we reported pasteurized AKK functions as novel postbiotics which ameliorated glucose intolerance and insulin resistance in the GDM mouse model induced by high-fat diet (HFD) feeding combined with STZ. The oral administration of pasteurized AKK enhanced glucose homeostasis and alleviated placental inflammation in the GDM mouse model. Specifically, placental macrophage polarization was transferred by AKK treatment. In addition, the outer membrane protein of AKK, Amuc_1100, mimicked anti-inflammatory properties and improvement of GDM, which served as an effector protein. These findings demonstrate that oral AKK supplementation alleviated placental inflammatory responses through modulating macrophage polarization. Mechanically, we uncovered that a heat-stable outer membrane protein of AKK, Amuc_1100, mimics the anti-diabetic properties of pasteurized AKK through oral administration. Taken together, our findings demonstrated an effective treatment of GDM from the perspective of potential probiotic agents.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101073"},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the protective effect of resveratrol-loaded solid lipid nanoparticles on radiation- or doxorubicin-induced spermatogenic damage in mice","authors":"Mohammad Farhadi Rad ,&nbsp;Faranak Aghaz ,&nbsp;Khodabakhsh Rashidi ,&nbsp;Sousan Gharibnejad ,&nbsp;Elham Arkan ,&nbsp;Kamran Mansouri ,&nbsp;Elahe Motevaseli ,&nbsp;Masoud Najafi","doi":"10.1016/j.repbio.2025.101058","DOIUrl":"10.1016/j.repbio.2025.101058","url":null,"abstract":"<div><div>Radiation and doxorubicin (Dox) exert destructive effects on testicular tissue. <em>Resveratrol</em>, a natural antioxidant, may protect the spermatogenic system against the toxic effects of these agents. This study evaluated the protective and antioxidant properties of resveratrol-loaded solid lipid nanoparticles (RES-SLNs) against Dox- and radiation-induced testicular injury in mice. Following the synthesis of RES-SLNs and characterization of their physicochemical properties, 60 adult male mice were randomly assigned to six groups (n = 10 per group): control, RES-SLNs, Dox, radiation (Rad), RES-SLNs + Dox, and RES-SLNs + Rad. Five mice from each group underwent anesthesia and bilateral orchiectomy for determination of malondialdehyde (MDA) and superoxide dismutase (SOD) levels. The remaining mice were sacrificed for histopathological evaluation of testicular tissue. Radiation and Dox administration significantly increased MDA levels, reduced SOD activity, and altered histological parameters. In contrast, intraperitoneal RES-SLN administration significantly lowered MDA levels, enhanced SOD activity, and mitigated morphological alterations induced by radiation or Dox. These findings indicate that RES-SLNs attenuate oxidative stress in the testes caused by radiation or Dox, thereby preventing severe structural damage.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101058"},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ameliorative effects of L-arginine on testicular and genotoxic toxicity induced by chronic exposure to cadmium chloride in male murine models","authors":"Nasibeh Hemati ,&nbsp;Milad Chahardori ,&nbsp;Mohammad Hosseinzadeh ,&nbsp;Fereshteh Taleb-Pour ,&nbsp;Fatemeh Shaki","doi":"10.1016/j.repbio.2025.101072","DOIUrl":"10.1016/j.repbio.2025.101072","url":null,"abstract":"<div><div>Cadmium (Cd) is a toxic heavy metal known to cause DNA damage, genetic alterations, and reproductive issues in males through free radical production. This study investigates L-arginine, an amino acid with antioxidant and metal-chelating properties, in mitigating Cd-induced genetic and reproductive toxicity in male mice. Thirty-six male mice were divided into six groups: a control group, a Cd group (2 mg/kg), three groups receiving Cd (2 mg/kg) combined with L-arginine (50, 100, 200 mg/kg), and a positive control group receiving vitamin C (500 mg/kg) with Cd. After 42 days, oxidative stress markers, including ROS, protein carbonyl, lipid peroxidation, and glutathione levels, were assessed in bone marrow and testicular tissue. Sperm parameters and histopathological evaluations of testes were also conducted. Chronic Cd exposure significantly reduced glutathione levels and increased oxidative stress markers in both tissues. Sperm count and motility decreased, while abnormal sperm increased. Conversely, L-arginine treatment notably reduced oxidative stress markers, improved antioxidant levels, sperm quality, and testicular histopathology compared to the Cd group. Additionally, Cd exposure increased the number of micronuclei in polychromatic erythrocytes (PCEs), which decreased with L-arginine treatment, enhancing the PCE/(PCE+normochromatic erythrocytes) ratio. These findings suggest that L-arginine effectively mitigates Cd-induced toxicity. The results indicate that L-arginine has significant protective effects against Cd-induced reproductive and bone marrow toxicity.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101072"},"PeriodicalIF":2.5,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fisetin attenuates the adverse effects of freezing and thawing procedures on the biological characteristics of human asthenoteratozoospermia samples","authors":"Daniyal Ezati ,&nbsp;Reyhane Vardiyan ,&nbsp;Ali Reza Talebi ,&nbsp;Fatemeh Alipour ,&nbsp;Hasan Pahang ,&nbsp;Shabnam Mohammadi","doi":"10.1016/j.repbio.2025.101060","DOIUrl":"10.1016/j.repbio.2025.101060","url":null,"abstract":"<div><div>Sperm cryopreservation is a conventional method to preserve sperm cells for a long time. This technique may cause various effects on sperm parameters. Therefore, applying beneficial antioxidants to decrease the adverse effects of freezing is noteworthy. Fisetin is a compound with antioxidant and anti-inflammatory properties. The main aim of the present study is to investigate the protective and beneficial effects of fisetin against cryo-damage on sperm functional parameters. In this experimental study, we analyzed 20 semen samples from asthenoteratozoospermic (AT) patients. Each sample was divided into three treatment groups: (1) fresh control (non-frozen), (2) cryopreservation medium (without fisetin), and (3) cryomedium supplemented with 50 μM fisetin. Freezing and thawing procedures were performed via the conventional method. Post-thaw analyses revealed that cryopreservation significantly reduced sperm motility, chromatin integrity, and mitochondrial membrane potential while increasing DNA fragmentation, malondialdehyde (MDA) levels, and apoptosis (p &lt; 0.05). Fisetin supplementation markedly improved progressive motility (p &lt; 0.05), reduced non-motile sperm percentage (p &lt; 0.05), and decreased DNA fragmentation and MDA levels (p &lt; 0.05). Additionally, it enhanced chromatin condensation and reduced apoptosis rates (p &lt; 0.05). Fisetin attenuates cryo-damage through its antioxidant and anti-apoptotic properties, improving post-thaw sperm quality. Thus, incorporating fisetin into cryopreservation media could enhance sperm viability for assisted reproductive technologies (ART).</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 4","pages":"Article 101060"},"PeriodicalIF":2.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}