Reproductive biology最新文献_第10页

Effect and mechanisms of cyclophosphamide-induced ovarian toxicity on the quality of primordial follicles with respect to age at treatment initiation 环磷酰胺引起的卵巢毒性对原始卵泡质量的影响和机制与开始治疗时的年龄有关

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-10-14 DOI: 10.1016/j.repbio.2024.100959

Motoki Takenaka , Hinako M. Takase , Noriko N. Suzuki , Chiemi Saigo , Tamotsu Takeuchi , Tatsuro Furui

{"title":"Effect and mechanisms of cyclophosphamide-induced ovarian toxicity on the quality of primordial follicles with respect to age at treatment initiation","authors":"Motoki Takenaka , Hinako M. Takase , Noriko N. Suzuki , Chiemi Saigo , Tamotsu Takeuchi , Tatsuro Furui","doi":"10.1016/j.repbio.2024.100959","DOIUrl":"10.1016/j.repbio.2024.100959","url":null,"abstract":"<div><div>Chemotherapy-induced ovarian toxicity in patients with cancer significantly affects future fertility depending on the age of initiation of treatment. However, the mechanisms underlying the age-related depletion of the ovarian reserve are not well understood. We investigated the effects of chemotherapy on pre- and postpubertal ovarian reserves in a mouse model. Juvenile (3-week-old) and adult (8-week-old) mice were injected with vehicle or cyclophosphamide (CPA;100 mg/kg). We assessed the short-term effects at 24 h and 72 h after injection and the long-term effects at 10 and 12 weeks of age by counting the follicles. The number of primordial follicles in the juvenile group was significantly reduced by CPA treatment compared with that in the adult group. To elucidate the mechanisms of this depletion, we performed immunostaining for γH2AX, cleaved PARP1, and FOXO3 at 24 h post-treatment. CPA-treated juvenile mice had a significantly higher proportion of γH2AX-positive primordial follicles, indicating double-strand DNA breaks. By contrast, 4-hydroperoxy CPA, an activated analog of CPA, induced γH2AX-positive primordial follicles in both groups <em>in vitro</em>, suggesting age-dependent differences in humoral ovarian microenvironment. Moreover, the level of cleaved PARP1 was specifically elevated in CPA-treated juvenile mice. However, primordial follicle activation was unaffected in the CPA-treated groups, as assessed by FOXO3 translocation. In conclusion, our findings suggest that ovaries in juveniles are more susceptible to DNA damage and subsequent apoptosis, leading to a higher rate of primordial follicle depletion. Therefore, it is crucial to recognize that cancer treatment, especially in children, can exert a substantial influence on future fertility.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100959"},"PeriodicalIF":2.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization and functional analysis of miR-92a-3p regulating Ino80d in mouse testis 小鼠睾丸中调控 Ino80d 的 miR-92a-3p 的定位和功能分析

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-10-13 DOI: 10.1016/j.repbio.2024.100961

Lvjing Luo , Lishuang Sun , Shu Li , Huiting Liu , Shi Huang , Yinyin Mo , Genliang Li

{"title":"Localization and functional analysis of miR-92a-3p regulating Ino80d in mouse testis","authors":"Lvjing Luo , Lishuang Sun , Shu Li , Huiting Liu , Shi Huang , Yinyin Mo , Genliang Li","doi":"10.1016/j.repbio.2024.100961","DOIUrl":"10.1016/j.repbio.2024.100961","url":null,"abstract":"<div><div>Testicular development and spermatogenesis in mice involve complex and dynamic gene regulation and chromatin remodelling. In this study, Real-time fluorescence quantitative PCR (RT-qPCR), Western Blot (WB), Immunofluorescence (IF), transfection and other techniques were used to analyse the expression of <em>Ino80d</em> mRNA and its encoded proteins in mouse testicular tissue and mouse spermatogonial cells, and to further analyse the possible target-regulatory relationship and function of miR-92a-3p and <em>Ino80d</em>. We found that <em>Ino80d</em> mRNA and protein expression was up-regulated in adult mouse testis tissue relative to juvenile mouse testis tissue, whereas miR-92a-3p expression was down-regulated in adult mouse testis tissue. Immunofluorescence results showed that the Ino80d protein was mainly localized in the nucleus of male germ cells. Ino80d protein expression is higher in spermatogonia, spermatid and lower in primary spermatocytes, secondary spermatocytes and sperm. There is a decreasing trend in development from spermatogonia to secondary spermatocytes. The transfection results showed that the expression levels of <em>Ino80d</em> mRNA and protein were down-regulated after overexpression of miR-92a-3p in mouse spermatogonia. Increased miR-92a-3p may be a key factor in inhibiting the expression of <em>Ino80d</em> mRNA and proteins in the miR-92a-3p mimics group of mouse spermatogonial cells, whereas differential expression may be a result of the negative regulation of miR-92a-3p, which regulates testicular development and spermatogenesis in mice.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring stem cell technology: Pioneering new pathways for female fertility preservation and restoration 探索干细胞技术：开辟女性生育能力保存和恢复的新途径。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-10-10 DOI: 10.1016/j.repbio.2024.100958

Ningjing Li , Xinrong Du , Yuhong Zhao , Qin Zeng , Changli Han , Dongsheng Xiong , Libing He , Guohui Zhang , Weixin Liu

{"title":"Exploring stem cell technology: Pioneering new pathways for female fertility preservation and restoration","authors":"Ningjing Li , Xinrong Du , Yuhong Zhao , Qin Zeng , Changli Han , Dongsheng Xiong , Libing He , Guohui Zhang , Weixin Liu","doi":"10.1016/j.repbio.2024.100958","DOIUrl":"10.1016/j.repbio.2024.100958","url":null,"abstract":"<div><div>The fertility of women is crucial for the well-being of individuals and families. However, various factors such as chemotherapy, lifestyle changes, among others, may lead to a decline in female fertility, thus emphasizing the significance of preserving and restoring fertility. Stem cells, with their unique capacity for self-renewal and pluripotent differentiation, have made significant strides in areas such as ovarian tissue cryopreservation, <em>in vitro</em> culture of frozen-thawed ovarian tissue, and construction of ovarian-like organs. This review aims to summarize the latest findings in these fields, highlighting the pivotal role, mechanisms, and future prospects of stem cell technology in preserving and restoring female fertility. Additionally, the importance of interdisciplinary collaboration is underscored, as personalized stem cell therapy regimens tailored through interdisciplinary cooperation between reproductive medicine and stem cell fields hold promise in providing reliable solutions for the preservation and restoration of female fertility.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100958"},"PeriodicalIF":2.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142407347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exposure to chronic stress impedes seasonal and gonadotropin-induced ovarian recrudescence in the gecko Hemidactylus frenatus 慢性应激会阻碍壁虎半爪蜥季节性和促性腺激素诱导的卵巢再发育。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-10-07 DOI: 10.1016/j.repbio.2024.100957

Ananya Ganeyan, C.B. Ganesh

{"title":"Exposure to chronic stress impedes seasonal and gonadotropin-induced ovarian recrudescence in the gecko Hemidactylus frenatus","authors":"Ananya Ganeyan, C.B. Ganesh","doi":"10.1016/j.repbio.2024.100957","DOIUrl":"10.1016/j.repbio.2024.100957","url":null,"abstract":"<div><div>The neuroendocrine regulation of the stress-reproductive axis in reptiles is complex due to the diverse reproductive strategies adopted by these animals. Consequently, the underlying mechanisms by which stress can affect the reproductive axis remain opaque in reptiles. In the present study, we examined the effect of stress on the seasonal and FSH-induced ovarian recrudescence during the breeding and non-breeding phases of the cycle in the tropical and subtropical house gecko <em>Hemidactylus frenatus.</em> During the recrudescence phase of the ovarian cycle, exposure of lizards to various stressors (handling, confinement, chasing, and noise) caused a significant increase in the percentage of corticotropin-releasing hormone (CRH) and adrenocorticotropic hormone (ACTH)-immunoreactive (ir) content in the median eminence (ME) and/or pars distalis of the pituitary gland (PD), concomitant with a significant decrease in the release of gonadotropin-releasing hormone (GnRH)-ir content into the ME and PD, and number of oogonia in the germinal bed and absence of the stage IV and V (vitellogenic) follicles in the ovary compared to experimental controls. During the non-breeding phase, treatment of stressed lizards with FSH did not stimulate the development of stage IV and V follicles, in contrast to their appearance in FSH-only-treated lizards. Collectively, these findings suggest that exposure to stressors prevents the seasonal ovarian recrudescence, possibly mediated through the suppression of hypothalamic GnRH release into the ME and PD and/or directly at the level of the ovary.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100957"},"PeriodicalIF":2.5,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intra-testicular visfatin inhibition disrupts androgen and estrogen signalling in the mouse testis 睾丸内的粘蛋白抑制会破坏小鼠睾丸中的雄激素和雌激素信号。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-09-28 DOI: 10.1016/j.repbio.2024.100956

Vanlal Rempuia, Guruswami Gurusubramanian, Vikas Kumar Roy

{"title":"Intra-testicular visfatin inhibition disrupts androgen and estrogen signalling in the mouse testis","authors":"Vanlal Rempuia, Guruswami Gurusubramanian, Vikas Kumar Roy","doi":"10.1016/j.repbio.2024.100956","DOIUrl":"10.1016/j.repbio.2024.100956","url":null,"abstract":"<div><div>Visfatin is expressed in the testis of chicken, humans and rodents; however, direct role of visfatin in the adult testis has not been studied. We investigated testicular responses after intra-testicular injection of FK866. The effects of visfatin inhibition were accessed at 24 hrs and 1 week post FK866 treatment. The testicular histoarchitecture were degenerated after 24 hrs of FK866 treatment along with supressed testosterone and proliferating markers and resumption in these parameters showed after 1 week. The expression of AR and ERα were down-regulated after 1 week of FK866 treatment. The expression of BCl2 was down-regulated along with a slight elevation of caspase3 after 24 hrs; however, both proteins still showed suppressed expression after 1 week. Furthermore, ERβ expression, 3βHSD, and 17βHSD were down-regulated in both groups compared to the control. Despite the down-regulation of some factors, the testicular proliferation and histoarchitecture showed resumption in the testis after 1 week of FK866 treatment. This could be due to increased testosterone secretion by suppressing aromatase expression. In conclusion, our result is the first report on the direct role of visfatin in the adult testis. Visfatin has a stimulatory role in testosterone synthesis and proliferation in the testis. Moreover, some deregulated factors in the testis after 1 week of FK866 treatment, despite normal histoarchitecture treatment, could be a compensatory mechanism after visfatin inhibitions.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100956"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hsa_circ_0043533 modulates apoptosis and viability of granulosa cells via miR-409-3p/BCL2 and EMT signalling in PCOS: Providing novel perspective of metformin Hsa_circ_0043533通过miR-409-3p/BCL2和EMT信号调节PCOS中颗粒细胞的凋亡和活力：提供二甲双胍的新视角。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-09-28 DOI: 10.1016/j.repbio.2024.100955

Jing Ma , Chang Liu , Huimin Zhang , Mingzi Zhao , Wenqian Zhu , Xin Du , Cuifang Hao

{"title":"Hsa_circ_0043533 modulates apoptosis and viability of granulosa cells via miR-409-3p/BCL2 and EMT signalling in PCOS: Providing novel perspective of metformin","authors":"Jing Ma , Chang Liu , Huimin Zhang , Mingzi Zhao , Wenqian Zhu , Xin Du , Cuifang Hao","doi":"10.1016/j.repbio.2024.100955","DOIUrl":"10.1016/j.repbio.2024.100955","url":null,"abstract":"<div><div>Polycystic ovary syndrome (PCOS) represents a significant cause of infertility among women of reproductive age. Studies have established a close association between granulosa cells (GCs) and the abnormal follicle formation and ovulation processes characteristic of PCOS. The interactions among hsa_circ_0043533, miR-409–3p, and BCL2 were verified through luciferase activity assays. In PCOS patients, granulosa cells exhibit notably reduced apoptosis but enhanced growth, leading to their accumulation and the development of polycystic ovaries. The involvement of non-coding RNAs in PCOS has been documented, with elevated levels of hsa_circ_0043533 observed in this condition. A comprehensive series of experiments were conducted to explore the role of hsa_circ_0043533 in PCOS and elucidate its underlying mechanisms. Silencing hsa_circ_0043533 was found to promote apoptosis and hinder the migration, proliferation, and viability of KGN cells. Furthermore, we uncovered the regulatory effects of hsa_circ_0043533 on the miR-409–3p/BCL2 axis and key markers of Epithelial-Mesenchymal Transition (EMT). Additionally, it was observed that metformin modulates the hsa_circ_0043533/miR-409–3p/BCL2 axis. Overall, this study provides novel insights into the molecular mechanisms regulating granulosa cell proliferation and apoptosis in PCOS, further elucidating the molecular pathogenesis of this condition.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100955"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated stress response mediates HSP70 to inhibit testosterone synthesis in aging testicular Leydig cells 综合应激反应介导 HSP70 抑制老化睾丸雷蒂格细胞中睾酮的合成。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-09-21 DOI: 10.1016/j.repbio.2024.100954

Junqiang Zhang , Hui Yu , Yongqi Fan , Longmei Wu , Yuan Fang , Zhaolian Wei , Zhiguo Zhang , Yunxia Cao

{"title":"Integrated stress response mediates HSP70 to inhibit testosterone synthesis in aging testicular Leydig cells","authors":"Junqiang Zhang , Hui Yu , Yongqi Fan , Longmei Wu , Yuan Fang , Zhaolian Wei , Zhiguo Zhang , Yunxia Cao","doi":"10.1016/j.repbio.2024.100954","DOIUrl":"10.1016/j.repbio.2024.100954","url":null,"abstract":"<div><div>The integrated stress response (ISR) is implicated in age-related diseases, while the molecular chaperone heat shock protein 70 (HSP70) can facilitate proper protein folding. However, the regulatory mechanism of ISR in insufficient testosterone synthesis of aging Leydig cells (LCs) remains unclear. This study aims to elucidate the regulatory role of ISR in inadequate testosterone synthesis of aging LCs. We observed a positive correlation between testosterone and HSP70 levels, which were found to be decreased in elderly men. ISR was detected in testicular tissue from old mice. The expression of testosterone synthesis related protein and the content of testosterone decreased in testicular tissue of old mice. Conversely, inhibition of the integrated stress response in testicular tissue led to an increase in steroid synthase expression among old mice. Furthermore, inhibiting ISR specifically within aging LCs resulted in enhanced protein translation efficiency and increased expression levels of new HSP70 and steroidogenic acute regulatory protein (StAR). These findings suggest that ISR occurrence within aging LCs affects StAR protein expression through regulation of HSP70-mediated translation, consequently impairing testosterone synthesis.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100954"},"PeriodicalIF":2.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1642431X24001001/pdfft?md5=66aa3d246d1285c9ecdebafddae586b4&pid=1-s2.0-S1642431X24001001-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abortion in AhR-knockout mice and fetomaternal immunity AhR基因敲除小鼠的流产与母体免疫力

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-09-18 DOI: 10.1016/j.repbio.2024.100952

Rikako Karube, Mebae Koike, Togo Ikuta, Kazuhiro Shiizaki

{"title":"Abortion in AhR-knockout mice and fetomaternal immunity","authors":"Rikako Karube, Mebae Koike, Togo Ikuta, Kazuhiro Shiizaki","doi":"10.1016/j.repbio.2024.100952","DOIUrl":"10.1016/j.repbio.2024.100952","url":null,"abstract":"<div><p>AhR knockout mice are not completely infertile; however, they do experience decreased litter sizes after repeated pregnancies. This study revealed that the decrease in the number of live births is partly due to fetal deaths leading to miscarriages. Interestingly, fetal mortality was found to be linked only to maternal <em>AhR</em> gene defects and not the fetal genotype. Furthermore, we observed no significant changes in litter sizes in allogenic pregnancy, where AhR-KO female mice were crossed with ICR male mice. The results indicated that the absence of AhR in the dams affected the expression of immune tolerance-related genes in both the placenta and fetus. Specifically, FoxP3 and indoleamine 2,3-dioxygenase-1 (IDO1) mRNA levels were lower in the placentas of AhR-KO dams than in those of wild-type dams. Moreover, there were elevated levels of IL-1β and IFN-γ mRNA in the placentas of the AhR-KO dams, which indicated increased inflammation. However, the mRNA expression levels of IL-6 and IDO1 were low despite the elevated mRNA levels of IL-1β and IFN-γ, which may be because AhR is directly involved in IL-6 and IDO1 transcription. These findings imply that in AhR-KO mice, fetal death may be attributed to the disturbance of fetal-maternal immune tolerance as a result of increased inflammation and reduced IDO1 and FoxP3 mRNA levels.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100952"},"PeriodicalIF":2.5,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1642431X24000986/pdfft?md5=b3231a49b7a80b295860eb82c5c14dca&pid=1-s2.0-S1642431X24000986-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-200c promotes trophoblast cell dysfunction via inhibition of PI3K/Akt signaling in unexplained recurrent spontaneous abortion 在原因不明的复发性自然流产中，MicroRNA-200c通过抑制PI3K/Akt信号传导促进滋养层细胞功能障碍。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-09-06 DOI: 10.1016/j.repbio.2024.100951

Lei Yue , Hui Xu

{"title":"MicroRNA-200c promotes trophoblast cell dysfunction via inhibition of PI3K/Akt signaling in unexplained recurrent spontaneous abortion","authors":"Lei Yue , Hui Xu","doi":"10.1016/j.repbio.2024.100951","DOIUrl":"10.1016/j.repbio.2024.100951","url":null,"abstract":"<div><p>Dysfunction in trophoblast cells is closely associated with the development of recurrent spontaneous abortion (RSA). Previous reports have indicated that microRNA (miR)−200c was upregulated in the serum of patients who have had abortions. This study aimed to investigate the regulatory effects and mechanisms of miR-200c in trophoblast cells. The human extravillous trophoblast cell line HTR-8/SVneo was either subjected to knockdown or overexpression of miR-200c, and its levels were measured using RT-qPCR. The cell behaviors of HTR-8/SVneo were assessed using CCK-8, Transwell, wound healing assays, and flow cytometry. Western blotting was used to detect the protein levels of Ki67, Bcl-2, Bax, MMP2/9, and PI3K/Akt-related markers. The findings revealed that miR-200c levels were higher in the villous tissues of URSA patients. Depletion of miR-200c impeded HTR-8/SVneo cell apoptosis and enhanced cell migration, invasiveness, and proliferation, while overexpression of miR-200c exhibited the opposite effects. The data suggested that mechanistically, miR-200c inactivated PI3K/Akt signaling in trophoblast cells. Furthermore, rescue experiments demonstrated that blocking PI3K/Akt signaling reversed the effects of miR-200c depletion on HTR-8/SVneo cell behavior. Therefore, miR-200c depletion can potentially improve trophoblast cell function by activating PI3K/Akt signaling.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100951"},"PeriodicalIF":2.5,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142147250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AOPPs induces EMT and fibrosis by activating oxidative stress through ERK/p38 MAPK signaling pathway in endometriosis AOPPs 通过 ERK/p38 MAPK 信号通路激活子宫内膜异位症中的氧化应激，从而诱导 EMT 和纤维化。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-09-05 DOI: 10.1016/j.repbio.2024.100950

Xiaoqing Luo , Sixi Wen , Junling Zeng , Jing Liu , Wenting Ye , Jiangpeng Wu , Songyu Huang , Wuwei Xie , Haiping Wen , Yan Sun , Jing Cai , Daidi Mo , Qianxia Lin , Mingwei Chen , Siyu Xia , Yali Song

{"title":"AOPPs induces EMT and fibrosis by activating oxidative stress through ERK/p38 MAPK signaling pathway in endometriosis","authors":"Xiaoqing Luo , Sixi Wen , Junling Zeng , Jing Liu , Wenting Ye , Jiangpeng Wu , Songyu Huang , Wuwei Xie , Haiping Wen , Yan Sun , Jing Cai , Daidi Mo , Qianxia Lin , Mingwei Chen , Siyu Xia , Yali Song","doi":"10.1016/j.repbio.2024.100950","DOIUrl":"10.1016/j.repbio.2024.100950","url":null,"abstract":"<div><p>Epithelial-mesenchymal transition (EMT) is known to play a crucial role in the development of endometriosis (EMs). However, the exact mechanisms involved in EMT regulation in EMs are not well understood. In this study, we performed comprehensive research using clinical samples, single-cell sequencing, and in vivo/in vitro models to investigate the effects of advanced oxidation protein products (AOPPs) on EMT and the underlying mechanisms in EMs. Combining bioinformatics analysis with experimental validation, our results show that AOPPs accumulate in EMs tissues, and their levels positively correlate with the expression of EMT markers in fibrotic lesions of EMs patients. Stimulation with AOPPs leads to a concentration- and time-dependent alteration of EMT markers expression in both in vitro and in vivo models. These effects are mainly mediated by the generation of reactive oxygen species and nitrite, along with the activation of the ERK and P38 signaling pathways. In chronic administration studies using normal rats, AOPPs induce EMT and enhance collagen deposition. These findings significantly contribute to our understanding of the molecular mechanisms of EMs and provide a foundation for future research and therapeutic development in this field.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100950"},"PeriodicalIF":2.5,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1642431X24000962/pdfft?md5=a467b2f44bd054b553343fdce1fd64f3&pid=1-s2.0-S1642431X24000962-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142147249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0