Reproductive biology最新文献_第3页

Presence of embryos increases uterine blood flow and affects endometrial gene expression in Holstein cows on day seven post-insemination 受精后第7天，胚胎的存在增加了子宫血流量，影响了荷斯坦奶牛子宫内膜基因的表达

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-30 DOI: 10.1016/j.repbio.2024.100986

Dragos Scarlet , Susanne E. Ulbrich , Michael Steufmehl , Änne Honnens , Heinrich Bollwein

{"title":"Presence of embryos increases uterine blood flow and affects endometrial gene expression in Holstein cows on day seven post-insemination","authors":"Dragos Scarlet , Susanne E. Ulbrich , Michael Steufmehl , Änne Honnens , Heinrich Bollwein","doi":"10.1016/j.repbio.2024.100986","DOIUrl":"10.1016/j.repbio.2024.100986","url":null,"abstract":"<div><div>Superovulation treatment leads to characteristic changes in uterine perfusion during the first three weeks of pregnancy in cattle, but the origin of these changes is unknown. We investigated embryo-induced effects on uterine blood flow volume (uBFV) and pulsatility index (uPI), as well as on steroid hormone concentrations during the first seven days after conception, together with endometrial gene expression at the time of embryo flushing, in Holstein Friesian cows. Animals (n = 8) underwent (a) artificial insemination (AI), (b) superovulation followed by artificial insemination (SOV-AI), and (c) superovulation followed by sham AI (SOV-control) in a Latin square design. Doppler ultrasound examinations and blood collections were performed 12 h before AI, as well as on days 1, 3, 5, and 7 post AI. Embryo flushing followed by endometrial biopsy collection was also performed on day 7 post AI. While uBFV was higher after SOV-AI compared to AI on days −0.5, 3, 5 and 7 post AI (<em>P</em> < 0.05), uPI was not affected (<em>P</em> > 0.05). Progesterone and total estrogen concentrations were increased following superovulation at all timepoints (<em>P</em> < 0.05) but were not affected by the presence of embryos (<em>P</em> > 0.05). Compared to AI treatment, SOV-AI was associated with increased <em>KDR</em> and decreased <em>NOS3</em> endometrial mRNA expression (both <em>P</em> < 0.05) on day 7 post AI. Taken together, our results demonstrate changes in uterine blood flow during the first seven days after AI, and in endometrial gene expression in response to the presence of embryos at this time.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100986"},"PeriodicalIF":2.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium triggers AMPK-mTOR pathway to modulate autophagy related to oxidative stress of sheep Leydig cells 硒触发 AMPK-mTOR 通路，调节与绵羊亮德细胞氧化应激有关的自噬作用

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-24 DOI: 10.1016/j.repbio.2024.100973

Liang Ma , Kexin Li , Yaru Guo , Jinyu Liu , Jianing Dong , Jun Li , Youshe Ren , Lei Shi

{"title":"Selenium triggers AMPK-mTOR pathway to modulate autophagy related to oxidative stress of sheep Leydig cells","authors":"Liang Ma , Kexin Li , Yaru Guo , Jinyu Liu , Jianing Dong , Jun Li , Youshe Ren , Lei Shi","doi":"10.1016/j.repbio.2024.100973","DOIUrl":"10.1016/j.repbio.2024.100973","url":null,"abstract":"<div><div>The objective of this study was to investigate the effect of oxidative stress induced by excessive Se on autophagy of sheep Leydig cells and its underlying mechanism. Leydig cells isolated from the testis of 8-month-old sheep were purified using a discontinuous Percoll density gradient. Cells were divided into four treatment groups (0, 2.0, 4.0 and 8.0 μmol/L of Se). After treatment with Se for 48 h, cell proliferation was detected by CCK-8 assay kit. The biochemical methods were used to evaluate the antioxidant status of Leydig cells. The mRNA transcript and protein abundance related to the AMPK-mTOR pathway and autophagy were detected by real-time PCR and western blot analysis.</div><div>The results showed that the Leydig cells treated with 8.0 μmol/L Se have the lowest cell viability. The greater ROS content and lower GSH-Px activity were also observed in the Se<sub>8.0</sub> group. The inclusion of 2.0 μmol/L Se in the medium did not affect the autophagy of Leydig cells. However, the relative abundance of ATG5 protein and LC3II/I ratio were elevated in the Se<sub>8.0</sub> group. Oxidative stress induced by excessive Se (8.0 μmol/L) dramatically improved the abundance of key proteins related to AMPK-mTOR pathway and led to an increase of phosphorylated AMPK, mTOR and ULK1. Compared with the Se<sub>8.0</sub> group, compound C could significantly inhibit the key molecules of AMPK-mTOR signaling pathway and mitigate the autophagy of Leydig cells induced by excessive Se. These results indicate that appropriate Se (2.0 μmol/L) can enhance the viability of sheep Leydig cells. Oxidative stress caused by Se excess can induce cell autophagy via activating AMPK-mTOR signaling pathway. The existed crosstalk between autophagy and apoptosis could decide the fate of Leydig cells. This process could play a decisive role in the maintenance of normal male fertility and spermatogenesis by affecting the number of Leydig cells in testis.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100973"},"PeriodicalIF":2.5,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Empagliflozin improves sperm quality in diabetic rats by lowering oxidative stress and reducing apoptosis-related genes expression Empagliflozin 可通过降低氧化应激和减少细胞凋亡相关基因的表达来改善糖尿病大鼠的精子质量。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-23 DOI: 10.1016/j.repbio.2024.100971

Mina Kiani, Malek Soleimani Mehranjani, Mohammad Ali Shariatzadeh

{"title":"Empagliflozin improves sperm quality in diabetic rats by lowering oxidative stress and reducing apoptosis-related genes expression","authors":"Mina Kiani, Malek Soleimani Mehranjani, Mohammad Ali Shariatzadeh","doi":"10.1016/j.repbio.2024.100971","DOIUrl":"10.1016/j.repbio.2024.100971","url":null,"abstract":"<div><div>Type 2 diabetes mellitus (T2DM) negatively impacts sperm health by increasing oxidative stress. Empagliflozin, a type of sodium-glucose cotransporter 2 (SGLT2) inhibitor, not only reduces blood glucose levels in T2DM but also has antioxidant properties. This study aimed to investigate the antioxidant effects of empagliflozin on sperm quality and spermatogenesis indices in rats with T2DM.Male rats were divided into three groups (n = 6): control, diabetes mellitus (DM, induced by streptozotocin + nicotinamide), and DM treated with empagliflozin (10 mg/kg, 56 days, via gavage). Sperm parameters including sperm count, viability, motility, normal morphology, DNA integrity, maturation, tail length, and daily sperm production were analyzed. Additionally, spermatogenesis indices, Bax/Bcl2 ratio and serum levels of superoxide dismutase (SOD), total antioxidant capacity (TAC), and malondialdehyde (MDA) were measured. In the DM group, there was a significant decrease in the mean sperm parameters, SOD, TAC, and spermatogenesis indices, along with a significant increase in the percentage of single-stranded DNA (AO<sup>+</sup>), immature sperm nuclei (AB<sup>+</sup>), MDA levels, and Bax/Bcl2 ratio, compared to the control group. In the DM + empagliflozin group, there was a significant increase in the mean sperm parameters, SOD levels (P˂0.001), and spermatogenesis indices. Additionally, there was a significant decrease in the mean AB+ (P˂0.01), AO<sup>+</sup>, MDA levels (P˂0.001), and Bax/Bcl2 ratio compared to the DM group. The results showed that empagliflozin can mitigate the adverse effects of diabetes and improve sperm quality and increase sperm production in diabetic rats.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100971"},"PeriodicalIF":2.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of tarantula cubensis extract on ischemia-reperfusion injury in rat ovaries: Immunohistochemical, biochemical and histopathological evaluations 狼蛛提取物对大鼠卵巢缺血再灌注损伤的保护作用：免疫组织化学、生物化学和组织病理学评估。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-21 DOI: 10.1016/j.repbio.2024.100970

Zahid Paksoy , Sevim Beyza Ozturk Sarikaya , Nihat Yumusak

{"title":"Protective effect of tarantula cubensis extract on ischemia-reperfusion injury in rat ovaries: Immunohistochemical, biochemical and histopathological evaluations","authors":"Zahid Paksoy , Sevim Beyza Ozturk Sarikaya , Nihat Yumusak","doi":"10.1016/j.repbio.2024.100970","DOIUrl":"10.1016/j.repbio.2024.100970","url":null,"abstract":"<div><div>Ischemia-reperfusion (IR) injury is a common pathology affecting the ovary. This study aimed to investigate the effect of Tarantula cubensis extract (TCE) on ovarian IR injury. Forty-two female rats were used and randomly divided into 7 groups: Control, Sham, IR, Ischemia+TCE+Reperfusion, TCE+IR, Ischemia+Ethanol+Reperfusion and Ethanol+IR. The control and sham groups did not receive any treatment. All other groups were subjected to 3 h of ischemia + 3 h of reperfusion. A single dose of TCE (0.2 ml Theranekron) was administered subcutaneously either before or after ischemia in the treatment groups. In the last 2 groups, ethanol was applied before or after ischemia. After reperfusion, blood samples were collected and analyzed for biochemical parameters. Additionally, the ovarian tissue was evaluated histopathologically and immunohistochemically. The IR group exhibited more marked hyperemia, hemorrhage, and necrosis. Both treatment groups showed histopathological improvement. Moreover, the recovery rate was greater in the animals given theranekron before ischemia. IR injury caused oxidative stress in the rats. However, Theranekron did not significantly reduce the MDA levels (p > 0.05). On the other hand, SOD levels were significantly increased in the treatment groups (p < 0.01). Compared to the control and sham groups, the IL-1β, IL-6 and TNF-α levels were very high in the IR group. Conversely, the immunoreactivity of these cytokines was further reduced in the animals given theranekron before ischemia. Our findings suggest that TCE may be beneficial in the treatment of ovarian IR damage, especially when Theranekron is given before ischemia.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100970"},"PeriodicalIF":2.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic psychological stress induces testicular oxidative stress affecting reproductive behavior in rats 慢性心理压力会诱发影响大鼠生殖行为的睾丸氧化应激。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-20 DOI: 10.1016/j.repbio.2024.100934

Tianfeng Yang , Xinye Liu , Chunyan Kang , Gonglin Hou , Yunyun Shen , Zheqi Liu

引用次数: 0

Unveiling Leydig cell heterogeneity and its role in male infertility: A single-cell transcriptomic study of human testicular tissue 揭示睾丸细胞异质性及其在男性不育症中的作用：人类睾丸组织的单细胞转录组研究。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-19 DOI: 10.1016/j.repbio.2024.100972

Liwei Zhou , Hanchao Liu , Yuming Chen , Lin Hua , Xiaolong Wu , Xintao Gao , Le Mao

{"title":"Unveiling Leydig cell heterogeneity and its role in male infertility: A single-cell transcriptomic study of human testicular tissue","authors":"Liwei Zhou , Hanchao Liu , Yuming Chen , Lin Hua , Xiaolong Wu , Xintao Gao , Le Mao","doi":"10.1016/j.repbio.2024.100972","DOIUrl":"10.1016/j.repbio.2024.100972","url":null,"abstract":"<div><div>Male infertility and impaired spermatogenesis are significant concerns in reproductive health, often linked to disruptions in the cellular and molecular processes within the testis. The cellular composition and transcriptional dynamics of human testicular tissue are crucial for understanding these issues. Previous studies have largely relied on bulk tissue analysis, which obscures the distinct roles and interactions of specific cell types. Here, through a comprehensive single-cell transcriptomic analysis of human testes across various developmental stages and pathological conditions, we reveal the intricate cellular heterogeneity and the molecular mechanisms underlying testicular function. Our study identifies significant disruptions in the differentiation trajectories of Germ cells in conditions such as Klinefelter syndrome (KS), AZFa deletion, and Sertoli-cell-only syndrome (SCOS). We further uncover key transcription factors and regulatory networks governing Leydig cell function, particularly those related to steroidogenesis and hormonal regulation. These findings highlight the organized yet complex cellular and molecular landscape of the testis and uncover critical pathways altered in male infertility. Collectively, our data suggest that targeted therapeutic strategies could be developed to address specific disruptions in testicular cell populations and their associated regulatory networks.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100972"},"PeriodicalIF":2.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the pathophysiology of polycystic ovary syndrome: Construction and analysis of a ceRNA network in cumulus cells 阐明多囊卵巢综合征的病理生理学：构建并分析积液细胞中的ceRNA网络

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-19 DOI: 10.1016/j.repbio.2024.100916

Jingjing Li , Li Fan , Jiajia Wei , Wenjie Huang

{"title":"Elucidating the pathophysiology of polycystic ovary syndrome: Construction and analysis of a ceRNA network in cumulus cells","authors":"Jingjing Li , Li Fan , Jiajia Wei , Wenjie Huang","doi":"10.1016/j.repbio.2024.100916","DOIUrl":"10.1016/j.repbio.2024.100916","url":null,"abstract":"<div><div>Polycystic Ovary Syndrome (PCOS) is a complex endocrine disorder with elusive molecular mechanisms. This study explores the competitive endogenous RNA (ceRNA) regulatory network in the cumulus cells of PCOS patients. ceRNAs are transcripts like mRNAs, miRNAs, and lncRNAs that competitively bind shared miRNAs, regulating gene expression post-transcriptionally. We analyzed mRNA, microRNA (miRNA), and long non-coding RNA (lncRNA) from two cohorts: 12 PCOS patients and 11 healthy controls (dataset GSE10946), and 5 PCOS patients and 5 healthy controls (dataset GSE72274). These microarray datasets, obtained from the Gene Expression Omnibus (GEO), helped us identify differentially expressed mRNAs, miRNAs, and lncRNAs. Our analysis revealed a significant ceRNA network, which may play a crucial role in the pathophysiology of PCOS. In this network, 5 lncRNAs, 3 miRNAs, and 36 mRNAs were identified as differentially expressed. These elements form a complex regulatory schema influencing key cellular processes related to the disease, such as cell cycle regulation and response to estrogen. The HOXA11-AS-hsa-miR-454–3p-CCND2 network emerged as a potentially valuable biomarker for PCOS diagnosis, supported by Receiver Operating Characteristic (ROC) curve analysis indicating strong predictive power. Our findings suggest that the ceRNA interactions in PCOS cumulus cells provide a deeper understanding of the disease's molecular basis and offer new avenues for therapeutic intervention. This in silico study lays the groundwork for further experimental validation of these ceRNA networks as targets for PCOS treatment.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100916"},"PeriodicalIF":2.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in fatty acids, vitamins, cholesterol and amino acid profiles of ram semen by freeze-thawing process 冻融过程中公羊精液脂肪酸、维生素、胆固醇和氨基酸谱的变化。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-19 DOI: 10.1016/j.repbio.2024.100953

İbrahim Halil Güngör , Recep Hakkı Koca , Serap Dayan Cinkara , Tutku Can Acısu , Figen Erdem Erişir , Gözde Arkalı , Şeyma Özer Kaya , Mustafa Sönmez , Seyfettin Gür , Ökkeş Yılmaz , Abdurrauf Yüce , Gaffari Türk

{"title":"Changes in fatty acids, vitamins, cholesterol and amino acid profiles of ram semen by freeze-thawing process","authors":"İbrahim Halil Güngör , Recep Hakkı Koca , Serap Dayan Cinkara , Tutku Can Acısu , Figen Erdem Erişir , Gözde Arkalı , Şeyma Özer Kaya , Mustafa Sönmez , Seyfettin Gür , Ökkeş Yılmaz , Abdurrauf Yüce , Gaffari Türk","doi":"10.1016/j.repbio.2024.100953","DOIUrl":"10.1016/j.repbio.2024.100953","url":null,"abstract":"<div><div>The objective of this study was to examine the impact of freeze-thawing on the levels of oxidative stress, fatty acids, vitamins A, D, E, and K, cholesterol, and amino acids, as well as on spermatological parameters, in ram semen. Semen was collected and pooled from each of the seven rams twice a week for three weeks. The mixed semen was diluted with tris + egg yolk diluent at 38 °C (Group 38 °C) and the temperature was reduced to 5 °C (Group 5 °C). Following the glycerolization-equilibration process (Group G-E), the samples were automatically frozen in liquid nitrogen vapor at −140 °C. The semen samples were thawed 24 h after freezing (Group Frozen-Thawed, F-T). A comparison of Group 38 °C with Group F-T revealed significant differences in several parameters. Motility rates, kinematic values, percentage of membrane integrity (HOS), some PUFA levels, ∑SFA and amino acid levels were significantly lower in Group F-T. Conversely, the ratio of dead and static spermatozoa, lipid peroxidation level, some PUFA levels, ∑MUFA, vitamins A, E and cholesterol levels were significantly higher in Group F-T. The majority of these alterations were also evident in semen samples subjected to G-E treatment. In conclusion, exposure of ram semen to G-E and F-T treatments results in modifications to semen, fatty acid, vitamin, and amino acid profiles, accompanied by elevated levels of lipid peroxidation. Moreover, this study demonstrated, for the first time, that oxidative stress was induced, some amino acid levels were altered, vitamin A and E levels were increased, vitamin D and K levels were not affected, and β-sitosterol levels were decreased after freeze-thawing in ram semen.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100953"},"PeriodicalIF":2.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FOXD1 activates KIFC1 to modulate aerobic glycolysis and reinforce cisplatin resistance of breast cancer FOXD1 激活 KIFC1 以调节有氧糖酵解并增强乳腺癌的顺铂耐药性。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-14 DOI: 10.1016/j.repbio.2024.100969

Haitao Gao, Jing Wang, Jiacai Liu, Huihua Wang, Tiantian Wang, Sha Li, Lili Niu, Ya Wei

{"title":"FOXD1 activates KIFC1 to modulate aerobic glycolysis and reinforce cisplatin resistance of breast cancer","authors":"Haitao Gao, Jing Wang, Jiacai Liu, Huihua Wang, Tiantian Wang, Sha Li, Lili Niu, Ya Wei","doi":"10.1016/j.repbio.2024.100969","DOIUrl":"10.1016/j.repbio.2024.100969","url":null,"abstract":"<div><h3>Background</h3><div>Breast cancer (BC) is the most prevalent invasive malignant tumor. Cisplatin (DDP) is a prototype of platinum-based chemotherapy drugs, its resistance severely hinders its clinical application. This project intended to figure out the exact mechanism of KIFC1 in the DDP resistance of BC.</div></div><div><h3>Methods</h3><div>The levels of KIFC1 and FOXD1 in BC as well as their binding sites were investigated by bioinformatics analysis. The signaling pathways regulated by FOXD1 were analyzed. Chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays verified the binding relationship between the two. Through quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot (WB), we assessed the expression of FOXD1, KIFC1, and glycolysis-related genes. CCK-8 assay was applied in the determination of cell viability to assess the efficacy of DDP resistance. Extracellular acidification rate (ECAR), glucose consumption, lactate synthesis, Adenosine triphosphate (ATP) content, and oxygen consumption rate (OCR) were measured to evaluate glycolysis.</div></div><div><h3>Results</h3><div>FOXD1 and KIFC1 were significantly upregulated in BC, with KIFC1 being significantly enriched in the glycolysis pathway. Overexpression of KIFC1 significantly enhanced the DDP resistance of BC cells, while promoting aerobic glycolysis. Mechanistically, FOXD1 was bound to the promoter of KIFC1 to activate its transcription. Its overexpression counteracted the inhibitory effect of KIFC1 knockdown on the DDP resistance of BC cells.</div></div><div><h3>Conclusion</h3><div>FOXD1 activates the glycolysis pathway by upregulating KIFC1, thereby facilitating BC cells’ DDP resistance. Therefore, the FOXD1/KIFC1 axis linked the glycolysis pathway to DDP resistance and may be a promising new target for reinforcing DDP resistance in BC.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"25 1","pages":"Article 100969"},"PeriodicalIF":2.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the impact of extracellular vesicle addition during IVM on the fertilization rate of equine oocytes following ICSI 研究在体外受精过程中添加细胞外囊泡对 ICSI 后马卵母细胞受精率的影响。

IF 2.5 3区生物学

Reproductive biology Pub Date : 2024-11-09 DOI: 10.1016/j.repbio.2024.100967

Julia Gabryś , Natalia Pietras , Wiktoria Kowal-Mierzwa , Elżbieta Karnas , Aneta Andronowska , Agnieszka Nowak , Joanna Kochan , Monika Bugno-Poniewierska

{"title":"Investigating the impact of extracellular vesicle addition during IVM on the fertilization rate of equine oocytes following ICSI","authors":"Julia Gabryś , Natalia Pietras , Wiktoria Kowal-Mierzwa , Elżbieta Karnas , Aneta Andronowska , Agnieszka Nowak , Joanna Kochan , Monika Bugno-Poniewierska","doi":"10.1016/j.repbio.2024.100967","DOIUrl":"10.1016/j.repbio.2024.100967","url":null,"abstract":"<div><div>The efficacy of in vitro embryo production (IVEP) in equines is relatively limited compared to other species due to the lack of a reliable superovulation technique, limited availability of cumulus oocyte complexes (COCs), low in vitro oocyte maturation (IVM) and fertilization rates. Extracellular vesicles (EVs), which are nanoparticles involved in intercellular signaling in the ovarian environment, have shown potential as supplements to improve oocyte development during IVM. This study tested the hypothesis that EVs from small (< 20 mm) ovarian follicles could enhance fertilization rates in mares. Follicular fluid was collected postmortem, and EVs were isolated and characterized. The IVM process was conducted with or without EVs (200 µg EV protein/ml). EV internalization during IVM was examined using fluorescent labeling and confocal microscopy. Following intracytoplasmic sperm injection (ICSI), presumptive zygotes were cultured in a time-lapse system. Confocal microscopy confirmed EV internalization by COCs. Nanoparticle tracking analysis showed that obtained EVs were submicron-sized, and flow cytometry identified surface markers CD81 and CD63 on a subpopulation of EVs. Transmission electron microscopy revealed the characteristic disk shape of EV isolates. After culture, 196 oocytes (36.84 %) exhibited a first polar body and were subjected to ICSI. The EV-treated group showed a significantly higher fertilization rate (34.7 % vs. 20.2 %; <em>P</em> < 0.05), reduced degeneration, and increased cleavage efficiency (<em>P</em> < 0.1). Despite early embryonic arrest in both groups, these results suggest that follicular fluid-derived EVs could play a supportive role in equine IVF procedures.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100967"},"PeriodicalIF":2.5,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0