乳杆菌在滋养细胞侵袭中的独特作用：与crispatus乳杆菌不同，iners乳杆菌不能促进HTR-8/SVneo细胞的侵袭

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology Pub Date : 2025-06-03 DOI:10.1016/j.repbio.2025.101041

Jonghyun Bae , Kazuhide Takada , Yoshinori Takeda , Quang Duy Trinh , Shihoko Komine-Aizawa , Dong Wook Kim , Satoshi Hayakawa

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引用次数: 0

摘要

在怀孕期间，乳杆菌在子宫中的作用尚不清楚。本研究旨在探讨乳杆菌对滋养细胞侵袭的影响。HTR-8/SVneo细胞系用L. iners （SLI）或L. crispatus （SLC）的培养上清液进行培养。SLC显著增强HTR-8/SVneo侵袭，同时基质金属蛋白酶-2 mRNA表达、蛋白表达和酶活性升高。相反，SLI不影响滋养细胞侵袭。这些结果表明，子宫内膜中乳杆菌的存在对促进妊娠早期滋养细胞侵袭的作用不如crispatus乳杆菌。

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Distinct roles of Lactobacillus species in trophoblast invasion: Lactobacillus iners fails to promote HTR-8/SVneo cell invasion unlike Lactobacillus crispatus

The role of Lactobacillus iners in the uterus during pregnancy is poorly understood. This study aimed to evaluate the effect of L. iners on trophoblast invasion. The HTR-8/SVneo cell line was cultured with culture supernatants from L. iners (SLI) or L. crispatus (SLC). SLC significantly enhanced HTR-8/SVneo invasion, which was accompanied by increased matrix metalloproteinase-2 mRNA expression, protein expression, and enzymatic activity. In contrast, SLI did not affect trophoblast invasion. These results suggest that the presence of L. iners in the endometrium is less favorable than that of L. crispatus for promoting trophoblast invasion during early pregnancy.

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来源期刊

Reproductive biology 生物-生殖生物学

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

29 days

期刊介绍： An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Poland. Reproductive Biology is an international, peer-reviewed journal covering all aspects of reproduction in vertebrates. The journal invites original research papers, short communications, review articles and commentaries dealing with reproductive physiology, endocrinology, immunology, molecular and cellular biology, receptor studies, animal breeding as well as andrology, embryology, infertility, assisted reproduction and contraception. Papers from both basic and clinical research will be considered.