Reproductive biology最新文献

{"title":"Hormonal dependent expression of apelin and apelin receptor in the ovary and uterus of mice","authors":"Borgohain Anima,&nbsp;Guruswami Gurusubramanian,&nbsp;Vikas Kumar Roy","doi":"10.1016/j.repbio.2024.100918","DOIUrl":"10.1016/j.repbio.2024.100918","url":null,"abstract":"<div><p>Apelin and APJ have been shown to regulate female reproductive functions. However, its uterine expression during the oestrous cycle and its regulation by ovarian steroids, along with gonadotropin regulation in the ovary, has not been investigated. This study aimed to analyze the steroid-dependent uterine expression of apelin/APJ in the uterus along with the oestrous cycle. Furthermore, it also aimed to investigate gonadotropin-dependent ovarian expression of apelin and APJ. To investigate the uterine expression of apelin and APJ during estrous cycle in mice, uterus at different estrous stage were collected. To explore the ovarian steroids dependent expression of apelin system in the uterus, ovariectomized mice were treated with only estrogen at dose of 30 ng/g, only progesterone at dose of 150 μg/g and combined doses. To study the effect of gonadotropin on ovarian expression of apelin system, immature mice were injected with 2.5 IU of pregnant mare serum gonadotropin (PMSG) alone and both PMSG plus 2.5 IU of chorionic gonadotropin (hCG). Apelin and APJ protein expression are modulated by estrous phases in the uterus. The uterine apelin and APJ expression are up-regulated by estrogen and down-regulated by progesterone. The expression and localization of APJ showed increased abundance in the follicles of PMSG treated mice, however, the PMSG plus HCG treatment showed formation of corpus luteum with increased abundance of APJ and progesterone secretion. The expression of apelin and APJ are regulated by pituitary gonadotropin in the ovary and uterine apelin system by ovarian steroid hormone.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 3","pages":"Article 100918"},"PeriodicalIF":2.5,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141461495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of USP22 ameliorates LPS-induced endometrial stromal cells inflammation and modulates cells decidualization by inhibiting ferroptosis","authors":"Xiuye Xing ,&nbsp;Guoli Zhang ,&nbsp;Fangjie Yi ,&nbsp;Xinghua Xu","doi":"10.1016/j.repbio.2024.100913","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100913","url":null,"abstract":"<div><p>Endometritis and the failure of decidualization of the endometrium are important factors contributing to the increased incidence of abortion. <em>USP22</em> is associated with various inflammatory diseases and has been shown to be involved in endometrial decidualization in mice. This study aims to investigate whether USP22 is involved in the regulation of inflammatory response and decidualization in human endometrial stromal cells (hESCs). In this study, lipopolysaccharide (LPS) was used to induce inflammation in hESCs, and MPA combined with cAMP was used to induce decidualization of hESCs. USP22 overexpression vector was constructed to study the role of USP22 in endometritis. The results showed that the USP22 protein and mRNA levels were decreased in LPS-induced hESCs. LPS induction increased the levels of TNF-α, IL-1β, and IL-6, as well as the expression of iNOS and COX2 proteins in hESCs. In the LPS group, the levels of F-actin, PRL, IGFBP1, SLC7A11, and GPX4 proteins decreased, while the levels of lipid peroxidation and total iron content increased. Additionally, the levels of ACSL4 and TFR1 proteins were up-regulated. Overexpression of USP22 reversed LPS-induced cellular inflammation, attenuated decidualization, and inhibited ferroptosis. However, the use of ferroptosis inducers diminished the regulatory effects of USP22 on inflammatory responses and decidualization. In summary, these suggested that USP22 reduces the LPS-induced inflammatory response and regulates the decidualization of hESCs, and possibly involving ferroptosis.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 3","pages":"Article 100913"},"PeriodicalIF":2.1,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in adiponectin levels of subclinical ketosis cows and their effects on steroid hormone secretion and proliferation in follicular granulosa cells","authors":"Chang Zhao ,&nbsp;BenZheng Jiang ,&nbsp;Weizhe Yan ,&nbsp;Xichun Wang ,&nbsp;Hongyan Ding ,&nbsp;Cheng Xia","doi":"10.1016/j.repbio.2024.100898","DOIUrl":"10.1016/j.repbio.2024.100898","url":null,"abstract":"<div><p>In dairy cows, the occurrence of subclinical ketosis (SCK) is particularly high during early lactation. Previously, we documented alterations in the abundance of adiponectin (ADPN) in anestrus cows with SCK in comparison to cows in estrus. In the present study, 60 cows were divided into two groups: control (C, <em>n</em> = 30) and SCK (<em>n</em> = 30). Based on cow’s estrus situation in two group at 55–60 days postpartum, 15 anestrus SCK cows and estrus cows were designated the SCK-A group and C-E group, respectively. The SCK-A group had downregulated serum and follicular fluid ADPN levels compared with the C-E group. The serum ADPN level was positively correlated with the insulin level and follicle growth rate, and there was a positive correlation between ADPN and glucose in the follicular fluid. Primary culture of dairy cow granulosa cells (GCs) was established to observe the effect of low glucose (Glu) and/or ADPN on GCs cyclins and proteins important for steroid synthesis. The results showed that the addition of 1 µg/mL ADPN alleviated the negative effects of low Glu treatment on the proliferation of GCs and the expression of steroid secretion related protein proteins. Treatment with LY294002 (PI3K inhibitor) four experimental GCs groups: control (0 µg/mL ADPN), 1 µg/mL ADPN, LY294002 inhibitor, and 1 µg/mL ADPN+LY294002. The results showed that ADPN promotes the secretion of steroid hormones by GCs through the PI3K–AKT. In summary, ADPN plays a crucial role in ameliorating postpartum anestrus in dairy cows with SCK.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 3","pages":"Article 100898"},"PeriodicalIF":2.1,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1642431X24000445/pdfft?md5=94306094f100ae1249ac9ae7a8863aae&pid=1-s2.0-S1642431X24000445-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of DIS3L in the initial segment is dispensable for sperm maturation in the epididymis and male fertility","authors":"Xiao Wang,&nbsp;Yan-Qin Feng,&nbsp;Hong Li,&nbsp;Yu Xu,&nbsp;Junjie Yu,&nbsp;Meiyang Zhou,&nbsp;Fanyi Qiu,&nbsp;Nana Li,&nbsp;Zhengpin Wang","doi":"10.1016/j.repbio.2024.100914","DOIUrl":"10.1016/j.repbio.2024.100914","url":null,"abstract":"<div><p>DIS3L, a catalytic exoribonuclease associated with the cytoplasmic exosome complex, degrades cytoplasmic RNAs and is implicated in cancers and certain other diseases in humans. Epididymis plays a pivotal role in the transport, maturation, and storage of sperm required for male fertility. However, it remains unclear whether DIS3L-mediated cytoplasmic RNA degradation plays a role in epididymis biology and functioning. Herein, we fabricated a <em>Dis3l</em> conditional knockout (<em>Dis3l</em> cKO) mouse line in which DIS3L was ablated from the principal cells of the initial segment (IS). Morphological analyses showed that spermatogenesis and IS differentiation occurred normally in <em>Dis3l</em> cKO mice. Additionally, the absence of DIS3L had no dramatic influence on the transcriptome of IS. Moreover, the sperm count, morphology, motility, and acrosome reaction frequency in <em>Dis3l</em> cKO mice were comparable to that of the control, indicating that the <em>Dis3l</em> cKO males had normal fertility. Collectively, our genetic model demonstrates that DIS3L inactivation in the IS is nonessential for sperm maturation and male fertility.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 3","pages":"Article 100914"},"PeriodicalIF":2.1,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141322265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory shift alterations of proinflammatory and anti-inflammatory cytokines in unexplained recurrent miscarriage patients","authors":"Pei-Yan Liang ,&nbsp;Ruochun Lian ,&nbsp;Lan Xiang ,&nbsp;Lili Shan ,&nbsp;Keren He ,&nbsp;Shan Wang","doi":"10.1016/j.repbio.2024.100911","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100911","url":null,"abstract":"<div><p>This study aimed to investigate the pro-inflammatory and anti-inflammatory cytokines status in the peripheral blood of uRM patients. The plasma pro-inflammatory (IFN-γ, IL-6, IL-1β, and TNF-α) and anti-inflammatory (TGF-β1, IL-10, and IL-4) cytokines of 25 patients with uRM were compared to 33 women with a successful pregnancy. It was concluded that patients with uRM have an excess pro-inflammatory cytokines status.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 3","pages":"Article 100911"},"PeriodicalIF":2.1,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of interleukin 6 (IL-6) on sperm quality, kinematic parameters, acrosome integrity, apoptosis, ultrastructure, and molecular docking in cryopreserved ram spermatozoa","authors":"Mahmoud A.E. Hassan ,&nbsp;Wael A. Khalil ,&nbsp;Aya A. Ismail ,&nbsp;Maha Abdullah Momenah ,&nbsp;Khaled M. Al-Marakby ,&nbsp;Sameh A. Abdelnour","doi":"10.1016/j.repbio.2024.100912","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100912","url":null,"abstract":"<div><p>Sperm cryopreservation can lead to subfertility due to potential damage to sperm DNA, membranes, and overall motility caused by the freeze-thaw process. Interleukin-6 (IL-6) is a versatile cytokine with various roles in reproductive processes. However, the impacts of IL-6 supplementation on cryopreserved ram sperm have not been thoroughly investigated. Therefore, this study aims to assess the influence of IL-6 on the sperm quality of cryopreserved ram sperm. Ram semen was collected, pooled, and extended with tris-citrate soybean lecithin extender supplemented with 0, 50, 100, and 200 ng/mL of IL-6. The samples experienced a standard freezing protocol, and sperm quality, kinematic parameters, ultrastructure, and molecular docking of cryopreserved ram spermatozoa were evaluated. The results showed that sperm kinematics, viability, progressive motility, and membrane integrity were significantly enhanced by the addition of 100 or 200 ng of IL-6/mL (<em>p</em> &lt; 0.05). Semen supplemented with 100 or 200 ng/mL of IL-6 also exhibited higher percentages of sperm kinematics, including DAP, DCL, DSL, VSL, VAP, VCL, and ALH, compared to other groups (<em>p</em> &lt; 0.05). IL-6 supplementation enhanced acrosome integrity, and reduced caspase-3 activity in post-thawed ram spermatozoa (<em>p</em> &lt; 0.05) compared to untreated group. Supplementation with IL-6 (200 ng/mL) significantly decreased oxidative biomarkers (NO, MDA, and H₂O₂) (<em>p &lt;</em> 0.001) and improved total antioxidant capacity (<em>p &lt;</em> 0.05). The percentage of sperm damage (tail, head, and midpiece) was significantly reduced by IL-6 supplementation (<em>p &lt;</em> 0.05). Electron micrographs showed that supplementation with 100 or 200 ng/mL IL-6 protected acrosome stability, plasma membrane integrity, and sustained the ultrastructure integrity of cryopreserved ram spermatozoa. The docking exploration indicates a higher binding affinity with sperm function biomarkers, including caspase 3, BCL2, and PSMA6, with binding energies of − 52.30 kcal/mol, − 56.04 kcal/mol, and − 57.06 kcal/mol, respectively. In conclusion, the addition of IL-6 to the freezing extender can enhance the post-thaw quality of cryopreserved ram spermatozoa.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 3","pages":"Article 100912"},"PeriodicalIF":2.1,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141290329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is renal dysfunction amplified in an arginine vasopressin induced rat model of preeclampsia?","authors":"Sapna Ramdin ,&nbsp;Thajasvarie Naicker ,&nbsp;Sooraj Baijnath ,&nbsp;Nalini Govender","doi":"10.1016/j.repbio.2024.100910","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100910","url":null,"abstract":"<div><p>Renal dysfunction is important in preeclampsia (PE) pathophysiology and has not been fully explored in the arginine vasopressin (AVP) rat model of PE. This study aimed to determine kidney toxicity associated with this model. Female Sprague Dawley rats (n = 24) were subcutaneously infused with AVP or saline for 18 days. Urine samples (GD8, 14 and 18) were used to determine the levels of albumin, VEGF-A, clusterin, NGAL/Lipocalin-2, KIM-1, cystatin C, TIMP-1, β2M and OPN <em>via</em> Multiplex ELISAs. Albumin, and NGAL/lipocalin-2 were significantly elevated in the PAVP <em>vs</em> PS group on GD14 and GD18 (<em>p</em> &lt; 0.001) respectively. VEGF-A significantly decreased in the pregnant <em>vs</em> non-pregnant groups on GD14 and 18 (<em>p</em> &lt; 0.001). Clusterin (<em>p</em> &lt; 0.001) and OPN (<em>p</em> &lt; 0.05) were significantly higher in the PAVP <em>vs</em> PS group on GD18. Cystatin C and KIM-1 are significantly upregulated in the PAVP <em>vs</em> PS groups throughout gestation (<em>p</em> &lt; 0.05). β2M is significantly elevated in the PAVP <em>vs</em> PS group on GD14 and 18 (<em>p</em> &lt; 0.05). AVP elevated the urinary levels of the kidney injury biomarkers and replicated the renal dysfunction associated with PE development. Our findings confirm the potential applications of this model in studying the mechanisms underlying renal damage in PE.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 3","pages":"Article 100910"},"PeriodicalIF":2.1,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1642431X24000561/pdfft?md5=adfdaaf7b72132b24fa100705cd2a8b5&pid=1-s2.0-S1642431X24000561-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141290328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rac1 promotes the lipopolysaccharide-induced inflammatory response and contraction-associated proteins (CAPs) expression in mouse uterine smooth muscle cells","authors":"Min Diao ,&nbsp;Yunkai Tao ,&nbsp;Qian Liu ,&nbsp;Lu Huang ,&nbsp;Hao Li ,&nbsp;Xuemei Lin","doi":"10.1016/j.repbio.2024.100896","DOIUrl":"https://doi.org/10.1016/j.repbio.2024.100896","url":null,"abstract":"<div><p>Activation of the maternal immune system leads to a downstream cascade of proinflammatory events that culminate in the activation of spontaneous uterine contractions, which is associated with preterm birth. Ras-related C3 botulinum toxin substrate 1 (Rac1) is a crucial protein related to cell contraction and inflammation. The main purpose of this study was to explore the role and function of Rac1′s regulation of inflammation through in- vivo and in-vitro experiments. Rac1 inhibitor was used in animal model of preterm birth and cells isolated from the uterine tissues of pregnant mice on gestational day 16 were transfected with adenovirus to knockdown or overexpress Rac1 and treated with the Calcium–calmodulin-dependent protein kinase II (CaMKII) inhibitor KN93. The expression of Rac1, uterine contraction-associated proteins (CAPs) (COX-2 and Connexin43), and inflammatory cytokines, were assessed by Western blotting and RT<img>PCR.</p><p>LPS upregulated Rac1, COX-2 and Connexin43 expression in uterine smooth muscle cells (USMCs). The expression of inflammatory cytokines, COX-2, and Connexin43 was significantly decreased in shRac1-transfected cells compared with cells stimulated with LPS only. Rac1 overexpression led to an increase in the expression of inflammatory cytokines, COX-2, and Connexin43. Furthermore, after Rac1 overexpression, KN93 reduced the expression of uterine contraction-associated proteins and inflammatory cytokines. It is thought that the effect of Rac1 on inflammatory cytokine and contraction-associated protein expression in USMCs is mediated by CaMKII. Rac1 can modulate the expression of contraction-associated proteins and inflammatory cytokines through the CaMKII pathway. Rac1 could be an effective therapeutic target for improving the outcome of preterm birth.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 3","pages":"Article 100896"},"PeriodicalIF":2.1,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141239963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “PFOS exposure destroys the integrity of the blood-testis barrier (BTB）through PI3K/AKT/mTOR-mediated autophagy”[Reprod Biol (2024) 24, 100846]","authors":"Zifeng Chen,&nbsp;Zhengru Chen,&nbsp;Sheng Gao,&nbsp;Jie Shi,&nbsp;Xinyao Li,&nbsp;Fei Sun","doi":"10.1016/j.repbio.2024.100892","DOIUrl":"10.1016/j.repbio.2024.100892","url":null,"abstract":"","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 2","pages":"Article 100892"},"PeriodicalIF":2.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1642431X2400038X/pdfft?md5=7f8943312e98dfde47bf7daf8c73d120&pid=1-s2.0-S1642431X2400038X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140913512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of folic acid supplementation and folate deficiency on embryo implantation","authors":"Elif Seda Özdemir ,&nbsp;Deniz İrem Bulut ,&nbsp;Atiye Seda Yar Sağlam ,&nbsp;Belgin İşgör ,&nbsp;Çiğdem Elmas","doi":"10.1016/j.repbio.2024.100895","DOIUrl":"10.1016/j.repbio.2024.100895","url":null,"abstract":"<div><p>Implantation is a critical stage of pregnancy, which occurs in a short period of interaction between the receptive endometrium and the embryo. Folic acid (FA) is a synthetic derivative of folate and is recommended as a pre-conceptional supplement. However, the impact of different doses of FA supplementation and folate deficiency during the early stages of pregnancy requires further investigation. The aim of this study was to investigate the possible effects of FA supplementation and folate deficiency on expression of Estrogen Receptor Alpha (ER-α), Vascular Endothelial Growth Factor-A (VEGFA), and Integrin alpha V and beta3 (Integrin αVβ3). A total of 32, 6–8-week old Wistar albino rats were divided into four groups of control, folate-deficiency, low-dose, and high-dose FA supplement groups. After five weeks of FA supplementation and folate deficiency model formation, mated rats were sacrificed on the 5th gestational day (GD), and implantation sites were collected. The expression of ER- α, VEGFA, and Integrin αVβ3 in the implantation sites were examined with immunohistochemistry and real-time PCR. The results revealed that the mRNA levels of ESR1, VEGFA, and Integrin αV and β3 were significantly increased in the high-dose FA group and significantly decreased in the folate deficiency group compared to the control group (p &lt; 0.05). Based on these results, it can be concluded that FA supplementation before pregnancy has positive effects on the maintenance of pregnancy, and folate deficiency may lead to implantation disorders.</p></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 3","pages":"Article 100895"},"PeriodicalIF":2.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}