Reproductive biology最新文献

{"title":"Therapeutic potential of diosgenin against methotrexate-induced testicular damage in the rat","authors":"Fatemeh Taleahmad ,&nbsp;Mohsen Khalili ,&nbsp;Narges Haddadzadeh-Niri ,&nbsp;Ensyie Joneidi ,&nbsp;Sara Taleahmad ,&nbsp;Mehrdad Roghani","doi":"10.1016/j.repbio.2024.100966","DOIUrl":"10.1016/j.repbio.2024.100966","url":null,"abstract":"<div><div>This study evaluated diosgenin effects on methotrexate-induced testicular injury in the rats. A single dose of methotrexate (MTX) (20 mg/kg, i.p) was administered, followed by two weeks of diosgenin treatment via gavage starting one day before methotrexate injection. Testicular damage was evaluated through histological examination of seminiferous tubules, as well as analysis of serum testosterone level, oxidative stress and inflammation biomarkers, and antioxidant levels. The results of this study showed that in the MTX-exposed group, oxidative stress indices of malondialdehyde (MDA), reactive oxygen species (ROS), nitrite and indices of inflammation consisting of tumor necrosis factor α (TNFα), and interleukin 6 (IL-6) have a significant increase compared to the control group. Additionally, reductions were observed in antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH). In addition, testosterone level decreased and signs of testicular damage were observed in the MTX group. Conversely, in the group treated with diosgenin alongside MTX at a dosage of 50 mg/kg, there was a significant decrease in oxidative stress markers (MDA, ROS, nitrite) and inflammatory markers (TNFα and IL-6). Moreover, there was a significant increase in the levels of antioxidant enzymes (SOD, CAT, and GSH). Diosgenin appears to have the potential to protect testicular tissue from damage caused by the toxic effects of MTX through the reduction of oxidative stress and inflammation.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100966"},"PeriodicalIF":2.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of human spermatozoa with different DNA fragmentation index using RNA sequencing","authors":"Kailin Yang ,&nbsp;Xue Sun ,&nbsp;Qiyuan Zheng ,&nbsp;Chen Pan ,&nbsp;Siyuan Wang ,&nbsp;Qingfang Lu ,&nbsp;Changlong Xu ,&nbsp;Yangqing Lu","doi":"10.1016/j.repbio.2024.100964","DOIUrl":"10.1016/j.repbio.2024.100964","url":null,"abstract":"<div><div>The study is aimed to screen the differential expressed genes (DEGs) related to sperm DNA fragmentation in men and provide reference basis of the sperm selection in assisted reproduction based on DNA fragmentation. We evaluated 60 semen samples from patients with high, medium or low sperm DNA fragmentation index (DFI). Using multicolor flow cytometry, we measured the content of reactive oxygen species (ROS), phosphatidylserine (PS) externalization and mitochondrial membrane potential (MMP) in these sperm samples. The results revealed that the more ROS content and PS externalization were detected in the sperm with higher DFI, but there was lower MMP level in the high DFI sperm. Next, we conducted RNA sequencing (RNA-seq) on 3 groups of sperm samples with high, medium and low DFI. Then, Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were performed on DEGs. Furthermore, we utilized qRT-PCR to validated the significantly DEGs from the RNA-seq assay. The transcriptome results showed a total of 5334 DEGs were found in the sperm sample with high, medium and low DFI. According to GO and KEGG analysis 421 down-regulated genes in the high DFI group were related to oxidative stress and spermatogenesis. Thirteen novel genes were also identified that most likely were involved in sperm DNA fragmentation, which were further validated by qRT-PCR. In conclusion, our study suggested that the sperms with highly fragmented DNA were accompanied by down-regulation of a series of genes related to antioxidant and spermatogenesis.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100964"},"PeriodicalIF":2.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From genome to epigenome: Who is a predominant player in the molecular hallmarks determining epigenetic mechanisms underlying ontogenesis?","authors":"Marcin Samiec ,&nbsp;Monika Trzcińska","doi":"10.1016/j.repbio.2024.100965","DOIUrl":"10.1016/j.repbio.2024.100965","url":null,"abstract":"<div><div>Genetic factors are one of the basic determinants affecting ontogenesis in mammals. Nevertheless, on the one hand, epigenetic factors have been found to exert the preponderant and insightful impact on the intracellular mechanistic networks related to not only initiation and suppression, but also up- and downregulation of gene expression in all the phases of ontogenetic development in a variety of mammalian species. On the other hand, impairments in the epigenetic mechanisms underlying reprogramming of transcriptional activity of genes (termed epimutations) not only give rise to a broad spectrum of acute and chronic developmental abnormalities in mammalian embryos, foetuses and neonates, but also contribute to premature/expedited senescence or neoplastic transformation of cells and even neurodegenerative and mental disorders. The current article is focused on the unveiling the present knowledge aimed at the identification, classification and characterization of epigenetic agents as well as multifaceted interpretation of current and coming trends targeted at recognizing the epigenetic background of proper ontogenesis in mammals. Moreover, the next objective of this paper is to unravel the mechanistic insights into a wide array of disturbances leading to molecular imbalance taking place during epigenetic reprogramming of genomic DNA. The above-indicated imbalance seems to play a predominant role in the initiation and progression of anatomo-, histo-, and physiopathological processes throughout ontogenetic development. Conclusively, different modalities of epigenetically assisted therapeutic procedures that have been exemplified in the current article, might be the powerful and promiseful tools reliable and feasible in the medical treatments of several diseases triggered by dysfunctions in the epigenetic landscapes, e.g., myelodysplastic syndromes or epilepsy.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100965"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The alteration in myometrial mRNA transcription of the regulatory genes of DNA methylation in mare with endometrosis","authors":"Beatriz Celeiro e Silva ,&nbsp;Ewa Monika Drzewiecka ,&nbsp;Katarzyna Piotrowska-Tomala ,&nbsp;Joana Alpoim-Moreira ,&nbsp;Agnieszka Sadowska ,&nbsp;Magdalena Karolina Kowalik ,&nbsp;Jorge Pimenta ,&nbsp;Maria Rosa Rebordão ,&nbsp;Graça Ferreira-Dias ,&nbsp;Dariusz Skarzynski ,&nbsp;Anna Szóstek-Mioduchowska","doi":"10.1016/j.repbio.2024.100962","DOIUrl":"10.1016/j.repbio.2024.100962","url":null,"abstract":"<div><div>A reduction in myometrial contractile activity can lead to inadequate cleaning of the uterine lumen, resulting in persistent endometritis and potentially endometrosis in mares. Oxytocin (OXT) is a key hormonal regulator of myometrial contraction. While epigenetic regulation of myometrial gene expression has been studied in humans, there is limited information on the expression of DNA methyltransferases (DNMTs) and ten-eleven translocation enzymes (TETs) in the myometrium of mares. This study aimed to evaluate the mRNA transcription of these enzymes and the potential role of DNA methylation in the expression of the OXT receptor (OXTR) gene in the myometrium of mares with endometrosis. Myometrial samples were collected post-mortem during the mid-luteal (n = 23) and follicular (n = 20) phases of the estrous cycle and assessed according to Kenney and Doig endometrial category (I, IIA, IIB, III). mRNA transcription of <em>OXTR, DNMT1</em>, <em>-3A</em>, -<em>3B</em> and <em>TET1</em>, <em>-2</em>, <em>-3</em> were determined using qPCR. DNA methylation analysis at CpG islands of OXTR exons 1 and 2 was performed using bisulfite pyrosequencing. Myometrial <em>OXTR</em> mRNA transcription and DNA methylation in its promoter region showed no significant differences between categories, although increased methylation was observed at CpG island position 6 in exon 2. <em>DNMT1, TET2</em>, and <em>TET3</em> mRNA transcription was altered in the equine myometrium depending on the phase of the estrous cycle and the severity of endometrosis (P &lt; 0.05). These findings indicate that DNMTs and TETs were expressed in myometrium in a manner specific to the severity of endometrosis and phases of the estrous cycle, suggesting a potential regulatory role in DNA methylation of myometrial gene expression.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100962"},"PeriodicalIF":2.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Negative effects of ketoprofen and meloxicam on distal cauda epidydimal duct contractions, testosterone levels, and sperm count in rats","authors":"Ramon Tadeu Galvão Alves Rodrigues ,&nbsp;Vitória Barros Marques ,&nbsp;Maria Santana da Silva ,&nbsp;Luana Talinne da Costa Gomes ,&nbsp;Maele Oliveira de Sena ,&nbsp;Bruna da Silva Figueiredo ,&nbsp;Jonas Ivan Nobre Oliveira ,&nbsp;Elaine Cristina Gavioli ,&nbsp;Danilo José Ayres de Menezes ,&nbsp;Edilson Dantas da Silva Junior","doi":"10.1016/j.repbio.2024.100963","DOIUrl":"10.1016/j.repbio.2024.100963","url":null,"abstract":"<div><div>Ketoprofen and meloxicam, non-steroidal anti-inflammatory drugs widely used in clinical practice, lack comprehensive investigation regarding their impact on male reproductive health, particularly on epididymal duct contractions and sperm parameters. Therefore, this study investigated the negative effects of ketoprofen or meloxicam on the contractions of the epididymal duct, sperm parameters, and serum testosterone levels in rats. Firstly, we assessed the <em>in vitro</em> effects of ketoprofen or meloxicam (1–100 μM) on the contractions of the epididymal duct elicited by noradrenaline. Rats were also orally treated with 5 mg/kg ketoprofen or 1 mg/kg meloxicam for 15 days following evaluation of epididymal duct contractions, sperm parameters, and serum testosterone levels. In vitro exposure to meloxicam (100 μM), but not ketoprofen, significantly reduced the maximum effect of noradrenaline in epididymal duct. Moreover, <em>in vivo</em> administration of ketoprofen and meloxicam decreased testosterone levels, sperm production, and sperm count in the caput/corpus region of the rat epididymis. Conversely, the sperm count in the cauda epididymis remained unchanged in animals treated with both ketoprofen and meloxicam. Meloxicam, but not ketoprofen, caused a delay in sperm transit time in the cauda region of the epididymis. <em>In vivo</em> treatment with both ketoprofen or meloxicam hindered the noradrenaline-induced contractions in the epididymal duct. In conclusion, ketoprofen and meloxicam can modify sperm parameters by decreasing testosterone levels and the contractions of the epididymal duct isolated from the distal cauda region of the rat epididymis.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100963"},"PeriodicalIF":2.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial biochemical and immunological characterization of an isolated 52.1 kDa pregnancy-associated glycoprotein charge variant","authors":"Gerardo Perera-Marín ,&nbsp;Giovanna León-Legaspi ,&nbsp;Everardo González-Padilla ,&nbsp;Clara Murcia ,&nbsp;Rogelio Alonso-Morales ,&nbsp;Silvia Ivonne Mora Herrera ,&nbsp;Griselda Valdez-Magaña","doi":"10.1016/j.repbio.2024.100960","DOIUrl":"10.1016/j.repbio.2024.100960","url":null,"abstract":"<div><div>Pregnancy-associated glycoproteins (PAGs) are synthesized in the placental cells of ruminants and are detectable in blood, milk, and urine. Many of these proteins have been obtained and characterized from placental extracts by precipitation with 80 % ammonium sulfate. The possibility of purifying PAGs by precipitation with other concentrations of ammonium sulfate remains unexplored. We aimed to study PAG proteins obtained from extracts of ovine placenta at 100 days of gestation through precipitation with 40 % ammonium sulfate (Extract 40). The main protein complex (130 kDa) was obtained after Extract 40 precipitation. Under reducing SDS-PAGE conditions, the 130 kDa complex dissociated in two PAG proteins with apparent molecular weights of 52.1 kDa and 26.1 kDa. The 130 kDa protein appeared to be a molecular complex consisting of two copies of the 52.1 kDa protein linked to one copy of the 26.1 kDa protein, presumably by disulfide bonds. Furthermore, the 52.1 kDa protein consisted of at least three isoforms with distinct isoelectric points. Amino acid microsequencing of the 52.1 kDa protein revealed a chimeric structure containing amino acid sequences of PAG1, PAG4, PAG6, and PAG1-like proteins. This procedure recovered a novel 130 kDa protein complex composed of 26.1 kDa and two 52.1 kDa PAGs. To the best of our knowledge, this has not been previously reported as heterologous polymeric molecules.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100960"},"PeriodicalIF":2.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect and mechanisms of cyclophosphamide-induced ovarian toxicity on the quality of primordial follicles with respect to age at treatment initiation","authors":"Motoki Takenaka ,&nbsp;Hinako M. Takase ,&nbsp;Noriko N. Suzuki ,&nbsp;Chiemi Saigo ,&nbsp;Tamotsu Takeuchi ,&nbsp;Tatsuro Furui","doi":"10.1016/j.repbio.2024.100959","DOIUrl":"10.1016/j.repbio.2024.100959","url":null,"abstract":"<div><div>Chemotherapy-induced ovarian toxicity in patients with cancer significantly affects future fertility depending on the age of initiation of treatment. However, the mechanisms underlying the age-related depletion of the ovarian reserve are not well understood. We investigated the effects of chemotherapy on pre- and postpubertal ovarian reserves in a mouse model. Juvenile (3-week-old) and adult (8-week-old) mice were injected with vehicle or cyclophosphamide (CPA;100 mg/kg). We assessed the short-term effects at 24 h and 72 h after injection and the long-term effects at 10 and 12 weeks of age by counting the follicles. The number of primordial follicles in the juvenile group was significantly reduced by CPA treatment compared with that in the adult group. To elucidate the mechanisms of this depletion, we performed immunostaining for γH2AX, cleaved PARP1, and FOXO3 at 24 h post-treatment. CPA-treated juvenile mice had a significantly higher proportion of γH2AX-positive primordial follicles, indicating double-strand DNA breaks. By contrast, 4-hydroperoxy CPA, an activated analog of CPA, induced γH2AX-positive primordial follicles in both groups <em>in vitro</em>, suggesting age-dependent differences in humoral ovarian microenvironment. Moreover, the level of cleaved PARP1 was specifically elevated in CPA-treated juvenile mice. However, primordial follicle activation was unaffected in the CPA-treated groups, as assessed by FOXO3 translocation. In conclusion, our findings suggest that ovaries in juveniles are more susceptible to DNA damage and subsequent apoptosis, leading to a higher rate of primordial follicle depletion. Therefore, it is crucial to recognize that cancer treatment, especially in children, can exert a substantial influence on future fertility.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100959"},"PeriodicalIF":2.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Localization and functional analysis of miR-92a-3p regulating Ino80d in mouse testis","authors":"Lvjing Luo ,&nbsp;Lishuang Sun ,&nbsp;Shu Li ,&nbsp;Huiting Liu ,&nbsp;Shi Huang ,&nbsp;Yinyin Mo ,&nbsp;Genliang Li","doi":"10.1016/j.repbio.2024.100961","DOIUrl":"10.1016/j.repbio.2024.100961","url":null,"abstract":"<div><div>Testicular development and spermatogenesis in mice involve complex and dynamic gene regulation and chromatin remodelling. In this study, Real-time fluorescence quantitative PCR (RT-qPCR), Western Blot (WB), Immunofluorescence (IF), transfection and other techniques were used to analyse the expression of <em>Ino80d</em> mRNA and its encoded proteins in mouse testicular tissue and mouse spermatogonial cells, and to further analyse the possible target-regulatory relationship and function of miR-92a-3p and <em>Ino80d</em>. We found that <em>Ino80d</em> mRNA and protein expression was up-regulated in adult mouse testis tissue relative to juvenile mouse testis tissue, whereas miR-92a-3p expression was down-regulated in adult mouse testis tissue. Immunofluorescence results showed that the Ino80d protein was mainly localized in the nucleus of male germ cells. Ino80d protein expression is higher in spermatogonia, spermatid and lower in primary spermatocytes, secondary spermatocytes and sperm. There is a decreasing trend in development from spermatogonia to secondary spermatocytes. The transfection results showed that the expression levels of <em>Ino80d</em> mRNA and protein were down-regulated after overexpression of miR-92a-3p in mouse spermatogonia. Increased miR-92a-3p may be a key factor in inhibiting the expression of <em>Ino80d</em> mRNA and proteins in the miR-92a-3p mimics group of mouse spermatogonial cells, whereas differential expression may be a result of the negative regulation of miR-92a-3p, which regulates testicular development and spermatogenesis in mice.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring stem cell technology: Pioneering new pathways for female fertility preservation and restoration","authors":"Ningjing Li ,&nbsp;Xinrong Du ,&nbsp;Yuhong Zhao ,&nbsp;Qin Zeng ,&nbsp;Changli Han ,&nbsp;Dongsheng Xiong ,&nbsp;Libing He ,&nbsp;Guohui Zhang ,&nbsp;Weixin Liu","doi":"10.1016/j.repbio.2024.100958","DOIUrl":"10.1016/j.repbio.2024.100958","url":null,"abstract":"<div><div>The fertility of women is crucial for the well-being of individuals and families. However, various factors such as chemotherapy, lifestyle changes, among others, may lead to a decline in female fertility, thus emphasizing the significance of preserving and restoring fertility. Stem cells, with their unique capacity for self-renewal and pluripotent differentiation, have made significant strides in areas such as ovarian tissue cryopreservation, <em>in vitro</em> culture of frozen-thawed ovarian tissue, and construction of ovarian-like organs. This review aims to summarize the latest findings in these fields, highlighting the pivotal role, mechanisms, and future prospects of stem cell technology in preserving and restoring female fertility. Additionally, the importance of interdisciplinary collaboration is underscored, as personalized stem cell therapy regimens tailored through interdisciplinary cooperation between reproductive medicine and stem cell fields hold promise in providing reliable solutions for the preservation and restoration of female fertility.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100958"},"PeriodicalIF":2.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142407347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to chronic stress impedes seasonal and gonadotropin-induced ovarian recrudescence in the gecko Hemidactylus frenatus","authors":"Ananya Ganeyan,&nbsp;C.B. Ganesh","doi":"10.1016/j.repbio.2024.100957","DOIUrl":"10.1016/j.repbio.2024.100957","url":null,"abstract":"<div><div>The neuroendocrine regulation of the stress-reproductive axis in reptiles is complex due to the diverse reproductive strategies adopted by these animals. Consequently, the underlying mechanisms by which stress can affect the reproductive axis remain opaque in reptiles. In the present study, we examined the effect of stress on the seasonal and FSH-induced ovarian recrudescence during the breeding and non-breeding phases of the cycle in the tropical and subtropical house gecko <em>Hemidactylus frenatus.</em> During the recrudescence phase of the ovarian cycle, exposure of lizards to various stressors (handling, confinement, chasing, and noise) caused a significant increase in the percentage of corticotropin-releasing hormone (CRH) and adrenocorticotropic hormone (ACTH)-immunoreactive (ir) content in the median eminence (ME) and/or pars distalis of the pituitary gland (PD), concomitant with a significant decrease in the release of gonadotropin-releasing hormone (GnRH)-ir content into the ME and PD, and number of oogonia in the germinal bed and absence of the stage IV and V (vitellogenic) follicles in the ovary compared to experimental controls. During the non-breeding phase, treatment of stressed lizards with FSH did not stimulate the development of stage IV and V follicles, in contrast to their appearance in FSH-only-treated lizards. Collectively, these findings suggest that exposure to stressors prevents the seasonal ovarian recrudescence, possibly mediated through the suppression of hypothalamic GnRH release into the ME and PD and/or directly at the level of the ovary.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"24 4","pages":"Article 100957"},"PeriodicalIF":2.5,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}