Respiratory Physiology & Neurobiology最新文献_第2页

Invasive ventilation at the boundary of viability: A respiratory pathophysiology study of infants born between 22 and 24 weeks of gestation 存活边界的侵入性通气：妊娠 22-24 周间出生婴儿的呼吸病理生理学研究。

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-09-03 DOI: 10.1016/j.resp.2024.104339

{"title":"Invasive ventilation at the boundary of viability: A respiratory pathophysiology study of infants born between 22 and 24 weeks of gestation","authors":"","doi":"10.1016/j.resp.2024.104339","DOIUrl":"10.1016/j.resp.2024.104339","url":null,"abstract":"<div><h3>Background</h3><p>Invasive ventilation of infants born before 24 weeks of gestation is critical for survival and long-term respiratory outcomes, but currently there is a lack of evidence to guide respiratory management. We aimed to compare respiratory mechanics and gas exchange in ventilated extremely preterm infants born before and after 24 weeks of gestation.</p></div><div><h3>Methods</h3><p>Secondary analysis of two prospective observational cohort studies, comparing respiratory mechanics and indices of gas exchange in ventilated infants born at 22–24 weeks of gestation (<em>N</em>=14) compared to infants born at 25–27 weeks (<em>N</em>=37). The ventilation/perfusion ratio (V<sub>A</sub>/Q), intrapulmonary shunt, alveolar dead space (V<sub>Dalv</sub>) and adjusted alveolar surface area (S<sub>A</sub>) were measured in infants born at the Neonatal Unit of King’s College Hospital NHS Foundation Trust, London, UK.</p></div><div><h3>Results</h3><p>Compared to infants of 25–27 weeks, infants of 22–24 weeks had higher median (IQR) intrapulmonary shunt [18 (4 - 29) % vs 8 (2 – 12) %, p=0.044] and higher V<sub>Dalv</sub> [0.9 (0.6 – 1.4) vs 0.6 (0.5 – 0.7) ml/kg, p=0.036], but did not differ in V<sub>A</sub>/Q. Compared to infants of 25–27 weeks, the infants of 22–24 weeks had a lower adjusted S<sub>A</sub> [509 (322- 687) vs 706 (564 - 800) cm<sup>2</sup>, p=0.044]. The infants in the two groups did not differ in any of the indices of respiratory mechanics.</p></div><div><h3>Conclusion</h3><p>Ventilated infants born before 24 completed weeks of gestation exhibit abnormal gas exchange, with higher alveolar dead space and intrapulmonary shunt and a decreased alveolar surface area compared to extreme preterms born after 24 weeks of gestation.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1569904824001320/pdfft?md5=3ee009b3a9626e7938120d0b544b5944&pid=1-s2.0-S1569904824001320-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of the pontine respiratory group in the suppression of cough by codeine in cats 可待因在抑制猫咳嗽中的脑桥呼吸群作用

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-08-28 DOI: 10.1016/j.resp.2024.104326

引用次数: 0

Recovery of ventilatory and metabolic responses to hypoxia in neonatal rats after chronic hypoxia 慢性缺氧后新生大鼠呼吸和代谢对缺氧反应的恢复

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-08-24 DOI: 10.1016/j.resp.2024.104317

{"title":"Recovery of ventilatory and metabolic responses to hypoxia in neonatal rats after chronic hypoxia","authors":"","doi":"10.1016/j.resp.2024.104317","DOIUrl":"10.1016/j.resp.2024.104317","url":null,"abstract":"<div><p>Chronic hypoxia (CH) during postnatal development attenuates the hypoxic ventilatory response (HVR) in mammals, but there are conflicting reports on whether this plasticity is permanent or reversible. This study tested the hypothesis that CH-induced respiratory plasticity is reversible in neonatal rats and investigated whether the initial plasticity or recovery differs between sexes. Rat pups were exposed to 3 d of normobaric CH (12 % O<sub>2</sub>) beginning shortly after birth. Ventilation and metabolic CO<sub>2</sub> production were then measured in normoxia and during an acute hypoxic challenge (12 % O<sub>2</sub>) immediately following CH and after 1, 4–5, and 7 d in room air. CH pups hyperventilated when returned to normoxia immediately following CH, but normoxic ventilation was similar to age-matched control rats within 7 d after return to room air. The early phase of the HVR (minute 1) was only blunted immediately following the CH exposure, while the late phase of the HVR (minute 15) remained blunted after 1 and 4–5 d in room air; recovery appeared complete by 7 d. However, when normalized to CO<sub>2</sub> production, the late phase of the hypoxic response recovered within only 1 d. The initial blunting of the HVR and subsequent recovery were similar in female and male rats. Carotid body responses to hypoxia (<em>in vitro</em>) were also normal in CH pups after approximately one week in room air. Collectively, these data indicate that ventilatory and metabolic responses to hypoxia recover rapidly in both female and male neonatal rats once normoxia is restored following CH.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung function parameters are associated with acute mountain sickness and are improved at high and extreme altitude 肺功能参数与急性高山病有关，在高海拔和极高海拔地区会得到改善。

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-08-23 DOI: 10.1016/j.resp.2024.104318

{"title":"Lung function parameters are associated with acute mountain sickness and are improved at high and extreme altitude","authors":"","doi":"10.1016/j.resp.2024.104318","DOIUrl":"10.1016/j.resp.2024.104318","url":null,"abstract":"<div><p>At altitude, factors such as decreased barometric pressure, low temperatures, and acclimatization might affect lung function.</p><p>The effects of exposure and acclimatization to high-altitude on lung function were assessed in 39 subjects by repetitive spirometry up to 6022 m during a high-altitude expedition. Subjects were classified depending on the occurrence of acute mountain sickness (AMS) and summit success to evaluate whether lung function relates to successful climb and risk of developing AMS.</p><p>Peak expiratory flow (PEF), forced vital capacity (FVC) and forced expiratory volume in 1 second (FEV1) increased with progressive altitude (max. +20.2 %pred, +9.3 %pred, and +6.7 %pred, all p<0.05). Only PEF improved with acclimatization (BC1 vs. BC2, +7.2 %pred, p=0.044). At altitude FEV1 (p=0.008) and PEF (p<0.001) were lower in the AMS group.</p><p>The risk of developing AMS was associated with lower baseline PEF (p<0.001) and longitudinal changes in PEF (p=0.008) and FEV1 (p<0.001). Lung function was not related to summit success (7126 m). Improvement in PEF after acclimatization might indicate respiratory muscle adaptation.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Airway mechanics alters generation of cough motor pattern 气道力学改变了咳嗽运动模式的产生。

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-08-14 DOI: 10.1016/j.resp.2024.104315

{"title":"Airway mechanics alters generation of cough motor pattern","authors":"","doi":"10.1016/j.resp.2024.104315","DOIUrl":"10.1016/j.resp.2024.104315","url":null,"abstract":"<div><p>Effects of sequential increase in airway resistance: no, low (5 kPa.s/l), high (24 kPa.s/l), and complete block in the inspiratory or expiratory phase of mechanically induced cough on the cough motor pattern were studied in 16 anesthetized (pentobarbital) spontaneously breathing cats (3.70±0.15 kg, 11♂, 5♀). Esophageal pressure and electromyographic activities of the diaphragm during inspiration and abdominal muscles during expiration were analyzed. No significant changes in the number of coughs occurred. Inspiratory occlusion caused a prolongation of cough inspiratory phase, cough inspiratory diaphragm activity, and all cough-related activity. Inspiratory occlusion along with high resistance increased inspiratory esophageal pressure amplitude, total cough cycle duration and the time between maximum activity of the diaphragm and abdominal muscles. High expiratory resistance and occlusion resulted in increased cough expiratory esophageal pressure amplitude, a longer active portion of cough expiration, and cough abdominal activity. Expiratory occlusion also prolonged cough expiratory phase, all cough activity, and total cough cycle. Significantly increased airway resistance and occlusion induce secondary, in addition to mechanical, changes in cough by significantly modulating the generated cough motor pattern. A certain level of resistance appears to be successfully compensated, resulting in minimal changes in coughing characteristics, including expiratory airflow and the rising time of the airflow. Afferent feedback from the respiratory tract, particularly volume feedback, represents a significant factor in modulating cough, mainly under various pathological conditions in the respiratory system.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Difference in expiratory flow limitations development in normoxia and hypoxia in healthy individuals 健康人在常氧和低氧条件下呼气流量限制发展的差异。

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-08-14 DOI: 10.1016/j.resp.2024.104316

{"title":"Difference in expiratory flow limitations development in normoxia and hypoxia in healthy individuals","authors":"","doi":"10.1016/j.resp.2024.104316","DOIUrl":"10.1016/j.resp.2024.104316","url":null,"abstract":"<div><p>The present study investigated the maintenance/repeatability of expiratory flow limitation (EFL) between normoxia and hypoxia.</p><p>Fifty-one healthy active individuals (27 men and 24 women) performed a lung function test and a maximal incremental cycling test in both normoxia and hypoxia (inspired oxygen fraction = 0.14) on two separate visits.</p><p>During exercise in normoxia, 28 participants exhibited EFL (55 %). In hypoxia, another cohort of 28 participants exhibited EFL. The two groups only partly overlapped.</p><p>Individuals with EFL only in normoxia reported lower maximal ventilation values in hypoxia than in normoxia (n=5; −13.5 ± 7.8 %) compared to their counterparts with EFL only in hypoxia (n=5; +6.7 ± 6.3 %) or without EFL (n=18; +5.1 ± 10.3 %) (p=0.004 and p<0.001, respectively).</p><p>EFL development may be induced by different mechanisms in hypoxia vs. normoxia since the individuals who exhibited flow limitation were not the same between the two environmental conditions. This change seems influenced by the magnitude of the maximal ventilation change.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1569904824001095/pdfft?md5=8fb0a78dbac47078e7fb442521270ba6&pid=1-s2.0-S1569904824001095-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141996343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardio-respiratory coupling and myocardial recovery in heart failure with reduced ejection fraction 射血分数降低型心力衰竭的心肺耦合和心肌恢复。

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-08-08 DOI: 10.1016/j.resp.2024.104313

{"title":"Cardio-respiratory coupling and myocardial recovery in heart failure with reduced ejection fraction","authors":"","doi":"10.1016/j.resp.2024.104313","DOIUrl":"10.1016/j.resp.2024.104313","url":null,"abstract":"<div><h3>Introduction</h3><p>The interaction between the cardiovascular and respiratory systems in healthy subjects is determined by the autonomic nervous system and reflected in respiratory sinus arrhythmia. Recently, another pattern of cardio-respiratory coupling (CRC) has been proposed linking synchronization of heart and respiratory system. However, CRC has not been studied precisely in heart failure (HF) with reduced ejection fraction (EF) (HFrEF) according to the myocardial recovery.</p></div><div><h3>Methods</h3><p>10-min resting electrocardiography measurements were performed in persistent HFrEF patients (n=40) who had a subsequent left ventricular EF (LVEF) of ≤ 40 %, HF with recovered EF patients (HFrecEF) (n=41) who had a subsequent LVEF of > 40 % and healthy controls (n=40). Respiratory frequency, respiratory rate, CRC index, time-domain, frequency-domain and nonlinear heart rate variability indices were obtained using standardized software-Kubios™. CRC index was defined as respiratory high-frequency peak minus heart rate variability high-frequency peak.</p></div><div><h3>Results</h3><p>Respiratory rate was positively correlated with high-frequency (HF) peak (Hz) in both persistent HFrEF group (p<0.001) and HFrecEF group (p<0.001), while respiratory rate was negatively correlated with HF power (ms<sup>2</sup>) in the healthy controls (p<0.05). CRC index was lowest in the persistent HFrEF group followed by HFrecEF and was high in healthy controls (0.008 vs 0.012 vs 0.056 Hz, p=0.03).</p></div><div><h3>Conclusion</h3><p>CRC index was lowest in patients with impaired myocardial recovery, which indicates that cardio-respiratory synchrony is stronger in persistent HFrEF. This may represent a higher HF peak (Hz)/lower HF power (ms<sup>2</sup>) and abnormal sympathovagal balance in persistent HFrEF group compared to healthy controls. Further work is underway to tests this hypothesis and determine the utility of CRC index in HF phenotypes and its utility as a potential biomarker of response with neuromodulation.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of positive allosteric modulation and orthosteric agonism of dopamine D2-like receptors on respiration in mouse models of Rett syndrome 多巴胺 D2 类受体的正异位调节和正异位激动对雷特综合征小鼠模型呼吸的影响

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-08-06 DOI: 10.1016/j.resp.2024.104314

{"title":"Effect of positive allosteric modulation and orthosteric agonism of dopamine D2-like receptors on respiration in mouse models of Rett syndrome","authors":"","doi":"10.1016/j.resp.2024.104314","DOIUrl":"10.1016/j.resp.2024.104314","url":null,"abstract":"<div><p>Rett syndrome (RTT) is an autism spectrum disorder caused by loss-of-function mutations in the methyl-CPG-binding protein 2 (<em>Mecp2</em>) gene. Frequent apneas and irregular breathing are prevalent in RTT, and also occur in rodent models of the disorder, including <em>Mecp2</em><sup>Bird</sup> and <em>Mecp2</em><sup>R168X</sup> mice. Sarizotan, a serotonin 5-HT1a and dopamine D2-like receptor agonist, reduces the incidence of apneas and irregular breathing in mouse models of RTT (Abdala et al., 2014). Targeting the 5HT1a receptor alone also improves respiration in RTT mice (Levitt et al., 2013). However, the contribution of D2-like receptors in correcting these respiratory disturbances remains untested. PAOPA, a dopamine D2-like receptor positive allosteric modulator, and quinpirole, a dopamine D2-like receptor orthosteric agonist, were used in conjunction with whole-body plethysmography to evaluate whether activation of D2-like receptors is sufficient to improve breathing disturbances in female heterozygous <em>Mecp2</em><sup>Bird/+</sup> and <em>Mecp2</em><sup>R168X/+</sup> mice. PAOPA did not significantly change apnea incidence or irregularity score in RTT mice. PAOPA also had no effect on the ventilatory response to hypercapnia (7 % CO<sub>2</sub>). In contrast, quinpirole reduced apnea incidence and irregularity scores and improved the hypercapnic ventilatory response in <em>Mecp2</em><sup>R168X/+</sup> and <em>Mecp2</em><sup>Bird/+</sup> mice, while also reducing respiratory rate. These results suggest that D2-like receptors could contribute to the positive effects of sarizotan in the correction of respiratory abnormalities in Rett syndrome. However, positive allosteric modulation of D2-like receptors alone was not sufficient to evoke these effects.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impaired diffusion at submaximal lung inflation in asthma and copd patients 哮喘和慢性阻塞性肺疾病患者肺部亚充气状态下的弥散功能受损。

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-08-02 DOI: 10.1016/j.resp.2024.104304

{"title":"Impaired diffusion at submaximal lung inflation in asthma and copd patients","authors":"","doi":"10.1016/j.resp.2024.104304","DOIUrl":"10.1016/j.resp.2024.104304","url":null,"abstract":"<div><h3>Introduction</h3><p>Dissolved-phase <sup>129</sup>Xe MRI metrics suggest that gas diffusion may be more compromised at submaximal lung inflation compared to maximal inflation. We hypothesized that this diffusion deficit could be detected by comparing the carbon monoxide transfer coefficient (Kco) at submaximal lung inflation to that measured routinely at total lung capacity (TLC).</p></div><div><h3>Methods</h3><p>Asthma and COPD patients performed carbon monoxide diffusion tests, first at maximal lung inflation for routine Kco and alveolar volume VA and then, at a 30 % reduced inflation (redux; obtaining Kco<sub>redux</sub> and VA<sub>redux</sub>). At both inflations mixing efficiency was determined as VA/TLC and VA<sub>redux</sub>/TLC<sub>redux</sub> to examine a potential effect on Kco<sub>redux</sub>/Kco behavior.</p></div><div><h3>Results</h3><p>In normal subjects (n=36), median Kco<sub>redux</sub>/Kco amounted to 130 [IQR:122–136]% as expected for normal Kco recruitment response. However, 60 % of asthma patients (49/83) and 80 % of COPD patients (44/55) showed reduced Kco recruitment at submaximal inflation (Kco<sub>redux</sub>/Kco<122 %). In the asthma group, with otherwise normal routine Kco, Kco<sub>redux</sub>/Kco was significantly correlated with RV/TLC ratio (r=-0.53;P<0.001), but not with VA/TLC. In COPD patients, all with abnormal routine Kco, abnormal Kco<sub>redux</sub>/Kco response occurred in those patients with lower FEV<sub>1</sub>, higher RV/TLC and lower VA/TLC (P<0.01 for all).</p></div><div><h3>Conclusion</h3><p>Sizeable portions of COPD and asthma patients showed a lack of normal Kco recruitment at submaximal lung inflation, related to high RV/TLC. In asthma, this was the case despite normal Kco at full lung inflation, suggesting that hyperinflation at lung volumes less than TLC affects the carbon monoxide diffusion rate constant by distorting pulmonary capillaries and alveolar–capillary membranes.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141890048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chloride intracellular channel 4 participates in the regulation of lipopolysaccharide-induced inflammatory responses in human bronchial epithelial cells 细胞内氯离子通道 4 参与调节脂多糖诱导的人类支气管上皮细胞炎症反应

IF 1.9 4区医学

Respiratory Physiology & Neurobiology Pub Date : 2024-07-17 DOI: 10.1016/j.resp.2024.104303

{"title":"Chloride intracellular channel 4 participates in the regulation of lipopolysaccharide-induced inflammatory responses in human bronchial epithelial cells","authors":"","doi":"10.1016/j.resp.2024.104303","DOIUrl":"10.1016/j.resp.2024.104303","url":null,"abstract":"<div><p>The airway epithelium is located at the interactional boundary between the external and internal environments of the organism and is often exposed to harmful environmental stimuli. Inflammatory response that occurs after airway epithelial stress is the basis of many lung and systemic diseases. Chloride intracellular channel 4 (CLIC4) is abundantly expressed in epithelial cells. The purpose of this study was to investigate whether CLIC4 is involved in the regulation of lipopolysaccharide (LPS)-induced inflammatory response in airway epithelial cells and to clarify its potential mechanism. Our results showed that LPS induced inflammatory response and decreased CLIC4 levels in vivo and in vitro. CLIC4 silencing aggravated the inflammatory response in epithelial cells, while overexpression of CLIC4 combined with LPS exposure significantly decreased the inflammatory response compared with cells exposed to LPS without CLIC4 overexpression. By labeling intracellular chloride ions with chloride fluorescent probe MQAE, we showed that CLIC4 mediated intracellular chloride ion-regulated LPS-induced cellular inflammatory response.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141639987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0