Research and Practice in Thrombosis and Haemostasis最新文献

{"title":"Silencing of the von Willebrand factor gene in proatherothrombotic APOE∗3-Leiden.CETP transgenic mice","authors":"Yvonne K. Jongejan ,&nbsp;Richard J. Dirven ,&nbsp;Elisa Schrader Echeverri ,&nbsp;Anke J.L. de Jong ,&nbsp;Amanda C.M. Pronk ,&nbsp;Sander Kooijman ,&nbsp;Patrick C.N. Rensen ,&nbsp;James E. Dahlman ,&nbsp;Jeroen C.J. Eikenboom ,&nbsp;Bart J.M. van Vlijmen","doi":"10.1016/j.rpth.2025.102699","DOIUrl":"10.1016/j.rpth.2025.102699","url":null,"abstract":"<div><h3>Background</h3><div>Elevated von Willebrand factor (VWF) levels correlate with higher risk of atherosclerosis-related arterial thrombosis (atherothrombosis). Silencing the <em>VWF</em> gene via small-interfering RNAs (siRNAs) could mitigate this risk. Previous studies successfully delivered siRNA to the endothelium of healthy, wild-type (WT) mice using lipid nanoparticles (LNPs).</div></div><div><h3>Objectives</h3><div>This study aimed to investigate whether the LNP-siRNA strategy could achieve endothelium-specific <em>Vwf</em>-silencing under diseased conditions of prolonged hypercholesterolemia and atherothrombosis-prone vasculature.</div></div><div><h3>Methods</h3><div>Female transgenic mice expressing a variant of human <em>APOE∗3</em> (ie, <em>APOE∗3-Leiden</em>) and human cholesteryl ester transfer protein (<em>CETP</em>), fed a cholesterol-enriched diet for 18 weeks, received an intravenous injection of LNP-encapsulated siRNA targeting <em>Vwf</em> (si<em>Vwf</em>) or scrambled control siRNA at 1.5 mg siRNA/kg. For comparison, the same LNP-siRNAs were administered to young, chow-fed WT mice. Plasma VWF and <em>Vwf</em> mRNA levels were measured 96 hours after injection, with immunofluorescence analysis of lungs and heart aortic root to assess VWF protein expression.</div></div><div><h3>Results</h3><div><em>APOE∗3-Leiden.CETP</em> mice exhibited elevated plasma VWF levels compared with WT mice, alongside hypercholesterolemia and aortic atherosclerosis. si<em>Vwf</em> administration led to over 85% reduction in plasma VWF in both strains, with a strong reduction in lung <em>Vwf</em> mRNA and VWF protein in the pulmonary endothelium. Similarly, si<em>Vwf</em> treatment resulted in the virtual absence of VWF protein in the endothelial lining of the aortic root of both nondiseased (WT mice) and atherosclerotic (<em>APOE∗3-Leiden.CETP</em> mice) vessel walls.</div></div><div><h3>Conclusion</h3><div>The LNP-siRNA targeting <em>Vwf</em> strongly reduced plasma and endothelial VWF in mice with hypercholesterolemia and advanced atherosclerosis, indicating feasibility to target endothelial VWF under proatherothrombotic conditions.</div></div>","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102699"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health literacy in pediatric thrombosis: a landscape analysis","authors":"Denise Bastas ,&nbsp;Athena Mancini ,&nbsp;Gina Wong ,&nbsp;Leonardo R. Brandão ,&nbsp;Sindi Mukaj ,&nbsp;Jennifer Vincelli ,&nbsp;Diandra Rollan ,&nbsp;Laura Avila","doi":"10.1016/j.rpth.2024.102653","DOIUrl":"10.1016/j.rpth.2024.102653","url":null,"abstract":"<div><h3>Background</h3><div>Health literacy can influence self-management, leading to improved health outcomes in pediatric patients with venous thrombotic events (VTEs).</div></div><div><h3>Objectives</h3><div>To assess general health literacy in adolescents and parents/caregivers of children diagnosed with VTE, and their perception and satisfaction with overall thrombosis-related knowledge, thrombosis knowledge compared to that of other conditions, and beliefs regarding thrombosis knowledge importance.</div></div><div><h3>Methods</h3><div>Patients aged 10 to 18 years with VTE history and parents/caregivers of patients aged 0 to 18 years with VTE attending clinic were recruited in this cross-sectional study. Health literacy was measured using the Rapid Estimate of Literacy in Medicine Short Forms (Adolescent and Adult), the Health Literacy Assessment Scale for Adolescents, and the e-Health Literacy Scale. Self-reported perception, satisfaction, comparative knowledge, and beliefs regarding thrombosis knowledge were assessed using researcher-generated questions.</div></div><div><h3>Results</h3><div>In total, 101 participants (50 adolescents, 51 parents/caregivers) were recruited at a median of 27 months (25th-75th percentile; 12-62 months) post-VTE diagnosis. Overall, 74% of adolescents and 59% of parents/caregivers had ≥1 measure indicating low general health literacy. Only half the participants thought their thrombosis knowledge was similar to that of other diseases. Satisfaction with thrombosis-related knowledge was 44%; 96% agreed that learning about thrombosis was important. Adolescents reported higher satisfaction with their knowledge than parents/caregivers, but satisfaction was not associated with demonstrated thrombosis knowledge.</div></div><div><h3>Conclusion</h3><div>Most participants had low general health literacy levels, and more than half were not satisfied with their thrombosis-related knowledge. Adolescents tended to overestimate their knowledge. Effective strategies to support health literacy in this population are needed.</div></div>","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102653"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11741949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma microRNA-145-5p as a diagnostic biomarker for acute deep vein thrombosis","authors":"Christopher Antoun ,&nbsp;Vårin Eiriksdatter Wikan ,&nbsp;Øyvind Øverli ,&nbsp;Thor Ueland ,&nbsp;Gholamreza Jafari Yeganeh ,&nbsp;Sigrid Kufaas Brækkan ,&nbsp;Ellen Brodin ,&nbsp;John-Bjarne Hansen","doi":"10.1016/j.rpth.2024.102671","DOIUrl":"10.1016/j.rpth.2024.102671","url":null,"abstract":"<div><h3>Background</h3><div>Identification of new biomarkers for acute deep vein thrombosis (DVT) that could lower the need for diagnostic imaging to confirm or rule out the diagnosis would be advantageous. microRNA-145-5p (miR-145) has the potential to be a diagnostic marker for acute DVT, but its diagnostic performance has not been evaluated in consecutive patients referred to the hospital with suspected DVT.</div></div><div><h3>Objectives</h3><div>The aim of this study was to assess the diagnostic performance of miR-145 for the diagnosis of acute lower extremity DVT.</div></div><div><h3>Methods</h3><div>Patients consecutively referred to the emergency room at Akershus University Hospital (Norway) due to suspicion of acute DVT between June 2021 and July 2023 were included. Within 24 hours of admission, blood was collected for assessment of plasma miR-145 (index test), and whole-leg compression ultrasound (reference standard) was used to confirm or rule out DVT. Cohen’s d effect size and area under the receiver operating curve (AUC) were used to estimate the diagnostic performance of miR-145 and D-dimer.</div></div><div><h3>Results</h3><div>Among 360 included patients, 101 (28%) were diagnosed with DVT. miR-145 showed poor diagnostic performance, as indicated by a Cohen’s d of −0.002 (95% CI, −0.241 to 0.216) and an AUC of 0.59 (95% CI, 0.51-0.67). For D-dimer, Cohen’s d was 1.66 (95% CI, 1.43-1.89), and the AUC was 0.92 (95% CI, 0.86-0.96).</div></div><div><h3>Conclusion</h3><div>Plasma levels of miR-145 showed poor diagnostic performance for lower extremity acute DVT among persons referred to the emergency room with clinical signs and symptoms of DVT.</div></div>","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102671"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thromboprophylaxis in multiple myeloma","authors":"Laurent Frenzel","doi":"10.1016/j.rpth.2025.102685","DOIUrl":"10.1016/j.rpth.2025.102685","url":null,"abstract":"","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102685"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond platelet activation: dysregulated lipid metabolism in defining risk and pathophysiology of VITT","authors":"Hannah Stevens ,&nbsp;James D. McFadyen ,&nbsp;Natalie A. Mellett ,&nbsp;David J. Lynn ,&nbsp;Thy Duong ,&nbsp;Corey Giles ,&nbsp;Jane James ,&nbsp;Rochelle Botten ,&nbsp;Georgina Eden ,&nbsp;Miriam Lynn ,&nbsp;Paul Monagle ,&nbsp;Peter J. Meikle ,&nbsp;Sanjeev Chunilal ,&nbsp;Karlheinz Peter ,&nbsp;Huyen Tran","doi":"10.1016/j.rpth.2025.102677","DOIUrl":"10.1016/j.rpth.2025.102677","url":null,"abstract":"<div><h3>Background</h3><div>VITT has emerged as a rare but serious adverse event linked primarily to adenoviral vector COVID-19 vaccinations, such as ChAdOx1-S (Oxford/AstraZeneca) vaccination. The syndrome is characterized by thrombosis with thrombocytopenia, elevated D-dimer, and pathologic platelet factor 4 antibodies within 42 days of vaccination.</div></div><div><h3>Objectives</h3><div>Despite dysregulated lipid metabolism underpinning many thrombotic conditions, the role of lipid alterations in VITT remains unexplored. Here, we examined the plasma lipidome of patients with VITT and compared it with those following ChAdOx1-S vaccination and with unprovoked venous thromboembolism (VTE) to understand the role of lipids in VITT pathophysiology.</div></div><div><h3>Methods</h3><div>This was a multicenter, prospective cohort study evaluating plasma lipidomics in newly diagnosed VITT samples, which were compared with both healthy controls following ChAdOx1-S vaccination and with unprovoked VTE.</div></div><div><h3>Results</h3><div>Comparison with ChAdOx1-S controls reveals a distinct lipid signature in VITT, characterized by elevations in phosphatidylserine and ceramide species, alongside reductions in several plasmalogens and acylcarnitine species. Notably, similarities between VITT lipid profiles and insulin resistance phenotypes suggest potential metabolic susceptibility. While few significant associations were found between VITT and VTE, an inverse correlation with several acylcarnitine species was demonstrated. Given the known anticoagulant role of acylcarnitine species, these findings suggest a plausible mechanistic pathway elevating the thrombotic potential of VITT above that of standard VTE.</div></div><div><h3>Conclusion</h3><div>These findings underscore the important role of lipid metabolism in VITT pathophysiology and highlight the complex interplay between lipids, coagulation, and pathologic thrombosis.</div></div>","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102677"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Should a history of venous thromboembolism be considered a contraindication for scuba diving?","authors":"Mathilde Vannini ,&nbsp;Jean-François Schved ,&nbsp;David M. Smadja ,&nbsp;Viktoria E.M. Jung ,&nbsp;Laetitia Mauge ,&nbsp;Olivier Sanchez ,&nbsp;Nicolas Gendron","doi":"10.1016/j.rpth.2024.102647","DOIUrl":"10.1016/j.rpth.2024.102647","url":null,"abstract":"<div><div>The question of whether scuba diving is safe for patients with a history of venous thromboembolism (VTE) remains unanswered. Cases of VTE have been reported after decompression accidents but not following properly conducted dives. However, the risk of VTE and bleeding on anticoagulant therapy during diving has yet to be defined. Medical diving societies make different recommendations regarding the risk of scuba diving in patients with a history of VTE and/or hereditary thrombophilia and regarding the bleeding risk under anticoagulation associated with dives. There is no epidemiologic or pathophysiologic evidence described in the literature to support a direct association between VTE and diving. Therefore, VTE cannot be considered as a definite contraindication for scuba diving. Further studies are needed to conclusively establish the risk of VTE associated with diving and to guide the development of medical guidelines by diving societies.</div></div>","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102647"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating tissue factor pathway inhibitor and other protease and protease inhibitors and their association with major adverse aortic events in patients with abdominal aortic aneurysm","authors":"Hamzah Khan ,&nbsp;Abdelrahman Zamzam ,&nbsp;Farah Shaikh ,&nbsp;Gustavo Saposnik ,&nbsp;Muhammad Mamdani ,&nbsp;Mohammad Qadura","doi":"10.1016/j.rpth.2024.102645","DOIUrl":"10.1016/j.rpth.2024.102645","url":null,"abstract":"<div><h3>Background</h3><div>Abdominal aortic aneurysm (AAA) is characterized by the proteolytic breakdown of the extracellular matrix, leading to dilatation of the aorta and increased risk of rupture. Biomarkers that can predict major adverse aortic events (MAAEs) are needed to risk stratify patients for more rigorous medical treatment and potential earlier surgical intervention.</div></div><div><h3>Objectives</h3><div>The primary objective was to identify the association between baseline levels of these biomarkers and MAAEs over a period of 5 years.</div></div><div><h3>Methods</h3><div>Baseline levels of 3 proteases (matrix metalloproteinases 7, 8, and 10) and 3 protease inhibitors (tissue factor pathway inhibitor [TFPI], SerpinA12, SerpinB3) were investigated. Plasma levels of these biomarkers were quantified in 134 patients with AAA and 134 matched controls. Patients were followed for a 5-year period during which MAAEs were documented. The association between these markers and MAAEs was evaluated using Cox regression and Kaplan–Meier survival curves.</div></div><div><h3>Results</h3><div>TFPI was significantly elevated in patients with AAA and significantly associated with MAAE during the 5-year period (hazard ratio, 1.52; 95% CI, 1.15-2.01; <em>P</em> = .003) after adjusting for covariates. Kaplan–Meier survival analyses demonstrated that patients in the high TFPI group (defined as plasma levels &gt;25.961 ng/mL) had significantly reduced freedom from the need for aortic repair and MAAEs.</div></div><div><h3>Conclusion</h3><div>These findings suggest that TFPI may serve as a valuable prognostic marker for the risk of MAAEs within 5 years in patients with AAA, potentially offering new tools for the medical management of patients with AAA.</div></div>","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102645"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and clinical impact of disseminated intravascular coagulation in acute aortic dissection: a nationwide cohort study","authors":"Shuhei Murao ,&nbsp;Yutaka Umemura ,&nbsp;Hirotaka Mori ,&nbsp;Yoshinobu Seki ,&nbsp;Takayuki Ikezoe ,&nbsp;Kohji Okamoto ,&nbsp;Satoshi Fujimi ,&nbsp;Kazuma Yamakawa","doi":"10.1016/j.rpth.2024.102656","DOIUrl":"10.1016/j.rpth.2024.102656","url":null,"abstract":"<div><h3>Background</h3><div>Acute aortic dissection is a life-threatening cardiovascular emergency with high mortality rates. Disseminated intravascular coagulation (DIC) is a critical complication in patients with acute aortic dissection; however, its incidence and impact on outcomes remain inconclusive.</div></div><div><h3>Objectives</h3><div>This study aimed to evaluate DIC prevalence and prognosis in patients with aortic dissection.</div></div><div><h3>Methods</h3><div>We conducted a multicenter retrospective cohort study using data from the Japan Medical Data Center claims database between 2014 and 2022. DIC was diagnosed based on the criteria of the Japanese Association for Acute Medicine (JAAM-2) and the International Society on Thrombosis and Haemostasis (ISTH). We compared the in-hospital mortality between patients with and without DIC and assessed the impact of coagulopathy using various coagulation profiles.</div></div><div><h3>Results</h3><div>Among the 3037 patients, 40% underwent surgery and 60% did not undergo surgery. The prevalence rates of JAAM-2 DIC and ISTH DIC were 21% and 9.4%, respectively. In-hospital mortality was significantly higher in the DIC group than in the non-DIC group, and this trend was consistently observed in the surgery and nonsurgery groups. Increased DIC scores correlated with higher in-hospital mortality. With the progression of coagulopathy, characterized by thrombocytopenia, elevated prothrombin time-international normalized ratio, prolonged activated partial thromboplastin time, increased D-dimer, and decreased fibrinogen levels, in-hospital mortality also increased.</div></div><div><h3>Conclusion</h3><div>The presence of DIC, as identified by both the JAAM-2 and ISTH criteria, was associated with increased in-hospital mortality in patients with acute aortic dissection. Therefore, further studies are needed to improve the clinical outcomes of these patients.</div></div>","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102656"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11774824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The detrimental impact of ferritin “normal” ranges on diagnosis of bleeding disorders in women","authors":"Michelle Sholzberg ,&nbsp;Grace H. Tang","doi":"10.1016/j.rpth.2024.102674","DOIUrl":"10.1016/j.rpth.2024.102674","url":null,"abstract":"","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102674"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrinsic pathway activation in patients with antiphospholipid syndrome and healthy controls","authors":"Dagmar J.M. van Mourik ,&nbsp;Valérie L.B.I. Jansen ,&nbsp;Michiel Coppens ,&nbsp;Saskia Middeldorp ,&nbsp;Hugo ten Cate ,&nbsp;Harry R. Büller ,&nbsp;Henri M.H. Spronk ,&nbsp;Magdolna Nagy ,&nbsp;Thijs E. van Mens","doi":"10.1016/j.rpth.2025.102694","DOIUrl":"10.1016/j.rpth.2025.102694","url":null,"abstract":"<div><h3>Background</h3><div>Antiphospholipid syndrome (APS) is a thrombotic autoimmune disease. Activation of the intrinsic coagulation pathway contributes to inflammatory and cardiovascular diseases, but its role in APS is unknown. Increased release of neutrophil extracellular traps and reduced effectiveness of direct oral anticoagulants support the hypothesis of increased intrinsic pathway activation in patients with APS, which is relevant considering the ongoing development and clinical testing of intrinsic pathway inhibitors.</div></div><div><h3>Objectives</h3><div>To compare <em>in vivo</em> intrinsic pathway activation of patients with APS and healthy controls.</div></div><div><h3>Methods</h3><div>Patients with APS without recent thrombotic or obstetric events and healthy controls were investigated. ELISAs were used to measure activated coagulation factors in complex with the natural inhibitors antithrombin or C1-esterase inhibitor in plasma. The primary outcome of this study was factor (F)XII activation, which initiates the intrinsic pathway. Secondary outcomes included activation of downstream intrinsic coagulation FXI and FIX.</div></div><div><h3>Results</h3><div>Plasma of 73 patients with APS and 19 healthy controls showed no significant difference in activated FXII-inhibitor complexes. The concentrations of activated FXI and FIX and inhibitor complexes likewise did not differ between the groups. A subanalysis of patients with APS by anticoagulant use showed no difference for FXII and FXI activation.</div></div><div><h3>Conclusion</h3><div>Intrinsic pathway activation in patients with APS without recent thrombotic or obstetric events did not differ significantly compared with healthy controls.</div></div>","PeriodicalId":20893,"journal":{"name":"Research and Practice in Thrombosis and Haemostasis","volume":"9 1","pages":"Article 102694"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}