Protein & Cell最新文献_第7页

METTL9-catalyzed histidine methylation of S100A9 suppresses the anti-Staphylococcus aureus activity of neutrophils. METTL9 催化的 S100A9 组氨酸甲基化抑制了中性粒细胞抗金黄色葡萄球菌的活性。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-29 DOI: 10.1093/procel/pwad047

Dan Cao, Mengyue Lv, Chi Hu, Shukai Li, Siwen Wang, Chao Xu, Wen Pan

引用次数: 0

The ubiquitin codes in cellular stress responses. 细胞应激反应中的泛素密码。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-29 DOI: 10.1093/procel/pwad045

Xiangpeng Sheng, Zhixiong Xia, Hanting Yang, Ronggui Hu

{"title":"The ubiquitin codes in cellular stress responses.","authors":"Xiangpeng Sheng, Zhixiong Xia, Hanting Yang, Ronggui Hu","doi":"10.1093/procel/pwad045","DOIUrl":"10.1093/procel/pwad045","url":null,"abstract":"Ubiquitination/ubiquitylation, one of the most fundamental post-translational modifications, regulates almost every critical cellular process in eukaryotes. Emerging evidence has shown that essential components of numerous biological processes undergo ubiquitination in mammalian cells upon exposure to diverse stresses, from exogenous factors to cellular reactions, causing a dazzling variety of functional consequences. Various forms of ubiquitin signals generated by ubiquitylation events in specific milieus, known as ubiquitin codes, constitute an intrinsic part of myriad cellular stress responses. These ubiquitination events, leading to proteolytic turnover of the substrates or just switch in functionality, initiate, regulate, or supervise multiple cellular stress-associated responses, supporting adaptation, homeostasis recovery, and survival of the stressed cells. In this review, we attempted to summarize the crucial roles of ubiquitination in response to different environmental and intracellular stresses, while discussing how stresses modulate the ubiquitin system. This review also updates the most recent advances in understanding ubiquitination machinery as well as different stress responses and discusses some important questions that may warrant future investigation.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":null,"pages":null},"PeriodicalIF":21.1,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10903993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9837931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term in vivo chimeric cells tracking in non-human primate. 非人灵长类动物体内长期嵌合细胞追踪。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-29 DOI: 10.1093/procel/pwad049

Junmo Wu, Yu Kang, Xiang Luo, Shaoxing Dai, Yuxi Shi, Zhuoyao Li, Zengli Tang, Zhenzhen Chen, Ran Zhu, Pengpeng Yang, Zifan Li, Hong Wang, Xinglong Chen, Ziyi Zhao, Weizhi Ji, Yuyu Niu

{"title":"Long-term in vivo chimeric cells tracking in non-human primate.","authors":"Junmo Wu, Yu Kang, Xiang Luo, Shaoxing Dai, Yuxi Shi, Zhuoyao Li, Zengli Tang, Zhenzhen Chen, Ran Zhu, Pengpeng Yang, Zifan Li, Hong Wang, Xinglong Chen, Ziyi Zhao, Weizhi Ji, Yuyu Niu","doi":"10.1093/procel/pwad049","DOIUrl":"10.1093/procel/pwad049","url":null,"abstract":"Non-human primates (NHPs) are increasingly used in preclinical trials to test the safety and efficacy of biotechnology therapies. Nonetheless, given the ethical issues and costs associated with this model, it would be highly advantageous to use NHP cellular models in clinical studies. However, developing and maintaining the naïve state of primate pluripotent stem cells (PSCs) remains difficult as does in vivo detection of PSCs, thus limiting biotechnology application in the cynomolgus monkey. Here, we report a chemically defined, xeno-free culture system for culturing and deriving monkey PSCs in vitro. The cells display global gene expression and genome-wide hypomethylation patterns distinct from monkey-primed cells. We also found expression of signaling pathways components that may increase the potential for chimera formation. Crucially for biomedical applications, we were also able to integrate bioluminescent reporter genes into monkey PSCs and track them in chimeric embryos in vivo and in vitro. The engineered cells retained embryonic and extra-embryonic developmental potential. Meanwhile, we generated a chimeric monkey carrying bioluminescent cells, which were able to track chimeric cells for more than 2 years in living animals. Our study could have broad utility in primate stem cell engineering and in utilizing chimeric monkey models for clinical studies.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":null,"pages":null},"PeriodicalIF":21.1,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10903985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41163976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-EM structure of cannabinoid receptor CB1-β-arrestin complex. 大麻素受体 CB1-β-restin 复合物的冷冻电镜结构。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-29 DOI: 10.1093/procel/pwad055

Yuxia Wang, Lijie Wu, Tian Wang, Junlin Liu, Fei Li, Longquan Jiang, Zhongbo Fan, Yanan Yu, Na Chen, Qianqian Sun, Qiwen Tan, Tian Hua, Zhi-Jie Liu

引用次数: 0

Ergothioneine and its congeners: anti-ageing mechanisms and pharmacophore biosynthesis. 麦角硫因及其同系物：抗衰老机制和药源生物合成。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-29 DOI: 10.1093/procel/pwad048

Li Chen, Liping Zhang, Xujun Ye, Zixin Deng, Changming Zhao

引用次数: 0

Correction to: EGFR signaling augments TLR4 cell surface expression and function in macrophages via regulation of Rab5a activation. 更正为表皮生长因子受体信号通过调节 Rab5a 的活化增强巨噬细胞中 TLR4 细胞表面的表达和功能。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-23 DOI: 10.1093/procel/pwae002

引用次数: 0

Anthrax lethal toxin and tumor necrosis factor-α synergize on intestinal epithelia to induce mouse death. 炭疽毒素和肿瘤坏死因子-α协同作用于肠上皮细胞诱导小鼠死亡。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-01 DOI: 10.1093/procel/pwad050

Xinhe Gao, Teng Teng, Yifei Liu, Tingting Ai, Rui Zhao, Yilong Fu, Peipei Zhang, Jiahuai Han, Yingying Zhang

{"title":"Anthrax lethal toxin and tumor necrosis factor-α synergize on intestinal epithelia to induce mouse death.","authors":"Xinhe Gao, Teng Teng, Yifei Liu, Tingting Ai, Rui Zhao, Yilong Fu, Peipei Zhang, Jiahuai Han, Yingying Zhang","doi":"10.1093/procel/pwad050","DOIUrl":"10.1093/procel/pwad050","url":null,"abstract":"Bacillus anthracis lethal toxin (LT) is a determinant of lethal anthrax. Its function in myeloid cells is required for bacterial dissemination, and LT itself can directly trigger dysfunction of the cardiovascular system. The interplay between LT and the host responses is important in the pathogenesis, but our knowledge on this interplay remains limited. Tumor necrosis factor-α (TNF-α) is a pleiotropic pro-inflammatory cytokine induced by bacterial infections. Since LT accumulates and cytokines, predominantly TNF, amass during B. anthracis infection, co-treatment of TNF + LT in mice was used to mimic in vivo conditions for LT to function in inflamed hosts. Bone marrow transplantation and genetically engineered mice showed unexpectedly that the death of intestinal epithelial cells (IECs) rather than that of hematopoietic cells led to LT + TNF-induced lethality. Inhibition of p38α mitogen-activated protein kinase (MAPK) signaling by LT in IECs promoted TNF-induced apoptosis and necroptosis of IECs, leading to intestinal damage and mouse death. Consistently, p38α inhibition by LT enhanced TNF-mediated cell death in human colon epithelial HT-29 cells. As intestinal damage is one of the leading causes of lethality in anthrax patients, the IEC damage caused by LT + TNF would most likely be a mechanism underneath this clinical manifestation and could be a target for interventions.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":null,"pages":null},"PeriodicalIF":21.1,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10833652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49681598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two antibodies show broad, synergistic neutralization against SARS-CoV-2 variants by inducing conformational change within the RBD. 两种抗体通过诱导 RBD 的构象变化，对 SARS-CoV-2 变体显示出广泛的协同中和作用。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-01 DOI: 10.1093/procel/pwad040

Hui Sun, Tingting Deng, Yali Zhang, Yanling Lin, Yanan Jiang, Yichao Jiang, Yang Huang, Shuo Song, Lingyan Cui, Tingting Li, Hualong Xiong, Miaolin Lan, Liqin Liu, Yu Li, Qianjiao Fang, Kunyu Yu, Wenling Jiang, Lizhi Zhou, Yuqiong Que, Tianying Zhang, Quan Yuan, Tong Cheng, Zheng Zhang, Hai Yu, Jun Zhang, Wenxin Luo, Shaowei Li, Qingbing Zheng, Ying Gu, Ningshao Xia

{"title":"Two antibodies show broad, synergistic neutralization against SARS-CoV-2 variants by inducing conformational change within the RBD.","authors":"Hui Sun, Tingting Deng, Yali Zhang, Yanling Lin, Yanan Jiang, Yichao Jiang, Yang Huang, Shuo Song, Lingyan Cui, Tingting Li, Hualong Xiong, Miaolin Lan, Liqin Liu, Yu Li, Qianjiao Fang, Kunyu Yu, Wenling Jiang, Lizhi Zhou, Yuqiong Que, Tianying Zhang, Quan Yuan, Tong Cheng, Zheng Zhang, Hai Yu, Jun Zhang, Wenxin Luo, Shaowei Li, Qingbing Zheng, Ying Gu, Ningshao Xia","doi":"10.1093/procel/pwad040","DOIUrl":"10.1093/procel/pwad040","url":null,"abstract":"Continual evolution of the severe acute respiratory syndrome coronavirus (SARS-CoV-2) virus has allowed for its gradual evasion of neutralizing antibodies (nAbs) produced in response to natural infection or vaccination. The rapid nature of these changes has incited a need for the development of superior broad nAbs (bnAbs) and/or the rational design of an antibody cocktail that can protect against the mutated virus strain. Here, we report two angiotensin-converting enzyme 2 competing nAbs-8H12 and 3E2-with synergistic neutralization but evaded by some Omicron subvariants. Cryo-electron microscopy reveals the two nAbs synergistic neutralizing virus through a rigorous pairing permitted by rearrangement of the 472-489 loop in the receptor-binding domain to avoid steric clashing. Bispecific antibodies based on these two nAbs tremendously extend the neutralizing breadth and restore neutralization against recent variants including currently dominant XBB.1.5. Together, these findings expand our understanding of the potential strategies for the neutralization of SARS-CoV-2 variants toward the design of broad-acting antibody therapeutics and vaccines.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":null,"pages":null},"PeriodicalIF":21.1,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10833452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9840864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 ORF8 does not function in the nucleus as a histone mimic. SARS-CoV-2 ORF8 在细胞核中不具有组蛋白模拟功能。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-01 DOI: 10.1093/procel/pwad042

Ping Liu, Junjie Hu, Lei Wang

引用次数: 0

Radiation injury and gut microbiota-based treatment. 辐射损伤和基于肠道微生物群的治疗。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-02-01 DOI: 10.1093/procel/pwad044

Weihong Wang, Bota Cui, Yongzhan Nie, Lijuan Sun, Faming Zhang

引用次数: 0