Protein & Cell最新文献

Setd2 overexpression rescues bivalent gene expression during SCNT-mediated ZGA.

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-13 DOI: 10.1093/procel/pwaf010

Xiaolei Zhang, Ruimin Xu, Yuyan Zhao, Yijia Yang, Qi Shi, Hong Wang, Xiaoyu Liu, Shaorong Gao, Chong Li

{"title":"Setd2 overexpression rescues bivalent gene expression during SCNT-mediated ZGA.","authors":"Xiaolei Zhang, Ruimin Xu, Yuyan Zhao, Yijia Yang, Qi Shi, Hong Wang, Xiaoyu Liu, Shaorong Gao, Chong Li","doi":"10.1093/procel/pwaf010","DOIUrl":"https://doi.org/10.1093/procel/pwaf010","url":null,"abstract":"Successful cloning through somatic cell nuclear transfer (SCNT) faces significant challenges due to epigenetic obstacles. Recent studies have highlighted the roles of H3K4me3 and H3K27me3 as potential contributors to these obstacles. However, the underlying mechanisms remain largely unclear. In this study, we generated genome-wide maps of H3K4me3 and H3K27me3 in mouse pre-implantation NT embryos. Our analysis revealed aberrantly over-represented broad H3K4me3 domain and H3K27me3 signal lead to increased bivalent marks at gene promoters in NT embryos compared with naturally fertilized (NF) embryos at the 2-cell stage, which may link to relatively low levels of H3K36me3 in NT 2-cell embryos. Notably, the overexpression of Setd2, a H3K36me3 methyltransferase, successfully restored multiple epigenetic marks, including H3K36me3, H3K4me3 and H3K27me3. Additionally, it reinstated the expression levels of ZGA-related genes by re-establishing H3K36me3 at gene body regions, which excluded H3K27me3 from bivalent promoters, ultimately improving cloning efficiency. These findings highlight the excessive bivalent state at gene promoters as a potent barrier and emphasize the removal of these barriers as a promising approach for achieving higher cloning efficiency.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143410165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Advances in gene and cellular therapeutic approaches for Huntington's disease.

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-12 DOI: 10.1093/procel/pwaf005

引用次数: 0

Antiviral activity of lipoxygenase against severe fever with thrombocytopenia syndrome virus. 脂氧合酶对严重发热伴血小板减少综合征病毒的抗病毒活性

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-01 DOI: 10.1093/procel/pwae061

Shuang Li, Xiaojie Zheng, Yunfa Zhang, Lingyu Zhang, Tong Yang, Hao Li, Caiyu Zhou, Xiao-Ai Zhang, Li-Zeng Gao, Wei Liu

引用次数: 0

RADICAL: a rationally designed ion channel activated by ligand for chemogenetics. RADICAL：通过配体激活的合理设计的离子通道，用于化学遗传学。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-01 DOI: 10.1093/procel/pwae048

Heng Zhang, Zhiwei Zheng, Xiaoying Chen, Lizhen Xu, Chen Guo, Jiawei Wang, Yihui Cui, Fan Yang

引用次数: 0

Lcn2 secreted by macrophages through NLRP3 signaling pathway induced severe pneumonia. 巨噬细胞通过 NLRP3 信号通路分泌的 Lcn2 可诱发重症肺炎。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-01 DOI: 10.1093/procel/pwae045

Mingya Liu, Feifei Qi, Jue Wang, Fengdi Li, Qi Lv, Ran Deng, Xujian Liang, Shasha Zhou, Pin Yu, Yanfeng Xu, Yaqing Zhang, Yiwei Yan, Ming Liu, Shuyue Li, Guocui Mou, Linlin Bao

引用次数: 0

Alzheimer's disease: insights into pathology, molecular mechanisms, and therapy. 阿尔茨海默病：对病理学、分子机制和治疗的见解。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-01 DOI: 10.1093/procel/pwae026

Qiuyang Zheng, Xin Wang

引用次数: 0

An upgraded nuclease prime editor platform enables high-efficiency singled or multiplexed knock-in/knockout of genes in mouse and sheep zygotes. 升级的核酸酶引物编辑平台能够高效地在小鼠和绵羊受精卵中进行单敲或多重敲入/敲除基因。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2025-01-20 DOI: 10.1093/procel/pwaf006

Weijia Mao,Pei Wang,Lei Zhou,Dongxu Li,Xiangyang Li,Xin Lou,Xingxu Huang,Feng Wang,Yanli Zhang,Jianghuai Liu,Yongjie Wan

引用次数: 0

FOXO3-engineered human mesenchymal stem cells efficiently enhance post-ischemic stroke functional rehabilitation. foxo3工程人间充质干细胞有效促进缺血性脑卒中后功能康复。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2025-01-17 DOI: 10.1093/procel/pwaf004

Fangshuo Zheng,Jinghui Lei,Zan He,Taixin Ning,Shuhui Sun,Yusheng Cai,Qian Zhao,Shuai Ma,Weiqi Zhang,Jing Qu,Guang-Hui Liu,Si Wang

引用次数: 0

The Icarian flight of antibody-drug conjugates: target selection amidst complexity and tackling adverse impacts. 抗体-药物偶联物的伊卡利亚飞行：复杂中的靶标选择和处理不利影响。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2025-01-15 DOI: 10.1093/procel/pwaf002

Han Liu,Hongye Zeng,Xiaojing Qin,Wenjing Ning,Lin Xu,Shiting Yang,Xue Liu,Wenxin Luo,Ningshao Xia

{"title":"The Icarian flight of antibody-drug conjugates: target selection amidst complexity and tackling adverse impacts.","authors":"Han Liu,Hongye Zeng,Xiaojing Qin,Wenjing Ning,Lin Xu,Shiting Yang,Xue Liu,Wenxin Luo,Ningshao Xia","doi":"10.1093/procel/pwaf002","DOIUrl":"https://doi.org/10.1093/procel/pwaf002","url":null,"abstract":"Antibody-drug conjugates (ADCs) represent a promising class of targeted cancer therapeutics that combine the specificity of monoclonal antibodies with the potency of cytotoxic payloads. Despite their therapeutic potential, the use of ADCs faces significant challenges, including off/on-target toxicity and resistance development. This review examines the current landscape of ADC development, focusing on the critical aspects of target selection and antibody engineering. We discuss strategies to increase ADC efficacy and safety, including multitarget approaches, pH-dependent antibodies, and masked peptide technologies. The importance of comprehensive antigen expression profiling in both tumor and normal tissues is emphasized, highlighting the role of advanced technologies, such as single-cell sequencing and artificial intelligence (AI), in optimizing target selection. Furthermore, we explore combination therapies and innovations in linker‒payload chemistry, which may provide approaches for expanding the therapeutic window of ADCs. These advances pave the way for the development of more precise and effective cancer treatments, potentially extending ADC applications beyond oncology.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":"127 1","pages":""},"PeriodicalIF":21.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin landscape alteration uncovers multiple transcriptional circuits during memory CD8+ T cell differentiation. 染色质景观改变揭示了记忆 CD8+ T 细胞分化过程中的多个转录回路。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-01-13 DOI: 10.1093/procel/pwaf003

Qiao Liu, Wei Dong, Rong Liu, Luming Xu, Ling Ran, Ziying Xie, Shun Lei, Xingxing Su, Zhengliang Yue, Dan Xiong, Lisha Wang, Shuqiong Wen, Yan Zhang, Jianjun Hu, Chenxi Qin, Yongchang Chen, Bo Zhu, Xiangyu Chen, Xia Wu, Lifan Xu, Qizhao Huang, Yingjiao Cao, Lilin Ye, Zhonghui Tang

{"title":"Chromatin landscape alteration uncovers multiple transcriptional circuits during memory CD8+ T cell differentiation.","authors":"Qiao Liu, Wei Dong, Rong Liu, Luming Xu, Ling Ran, Ziying Xie, Shun Lei, Xingxing Su, Zhengliang Yue, Dan Xiong, Lisha Wang, Shuqiong Wen, Yan Zhang, Jianjun Hu, Chenxi Qin, Yongchang Chen, Bo Zhu, Xiangyu Chen, Xia Wu, Lifan Xu, Qizhao Huang, Yingjiao Cao, Lilin Ye, Zhonghui Tang","doi":"10.1093/procel/pwaf003","DOIUrl":"https://doi.org/10.1093/procel/pwaf003","url":null,"abstract":"Extensive epigenetic reprogramming involves in memory CD8+ T-cell differentiation. The elaborate epigenetic rewiring underlying the heterogeneous functional states of CD8+ T cells remains hidden. Here, we profile single-cell chromatin accessibility and map enhancer-promoter interactomes to characterize the differentiation trajectory of memory CD8+ T cells. We reveal that under distinct epigenetic regulations, the early activated CD8+ T cells divergently originated for short-lived effector and memory precursor effector cells. We also uncover a defined epigenetic rewiring leading to the conversion from effector memory to central memory cells during memory formation. Additionally, we illustrate chromatin regulatory mechanisms underlying long-lasting versus transient transcription regulation during memory differentiation. Finally, we confirm the essential roles of Sox4 and Nrf2 in developing memory precursor effector and effector memory cells, respectively, and validate cell state-specific enhancers in regulating Il7r using CRISPR-Cas9. Our data pave the way for understanding the mechanism underlying epigenetic memory formation in CD8+ T-cell differentiation.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0