Protein & Cell最新文献_第2页

Structural basis of arpin homodimerization reveals cooperative inhibition of the Arp2/3 complex through dual-site engagement. arpin同型二聚化的结构基础揭示了通过双位点接合来协同抑制Arp2/3复合物。

IF 12.8 1区生物学

Protein & Cell Pub Date : 2026-03-24 DOI: 10.1093/procel/pwag021

Liru Liu, Xuejiao Zhang, Yun Zhu, Mengchen Pu, Yuru Geng, Min Wang, Rongguang Zhang, Shen Ge, Sheng Ye

引用次数: 0

Hemoglobin-based oxygen carrier: What we have learned and where we are heading? 基于血红蛋白的氧载体：我们学到了什么，我们将走向何方？

IF 12.8 1区生物学

Protein & Cell Pub Date : 2026-03-24 DOI: 10.1093/procel/pwag019

Han Xiao, Weiliang Xia

引用次数: 0

Restoring the FOXO1 geroprotective pathway via seno-resistant mesenchymal progenitor cells alleviates primate epididymal aging. 通过抗衰老间充质祖细胞恢复FOXO1衰老保护通路可减轻灵长类附睾衰老。

IF 12.8 1区生物学

Protein & Cell Pub Date : 2026-03-24 DOI: 10.1093/procel/pwag020

Huifen Lu, Linguo Cai, DongLiang Lv, Guoqiang Sun, Jinghui Lei, Taixin Ning, Zijuan Xin, Haoyan Huang, Ying Jing, Daoyuan Huang, Shuhui Sun, Shuai Ma, Weiqi Zhang, Fei Gao, Rui Chen, Yingying Qin, Weihong Song, Andy Peng Xiang, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Jing Qu, Si Wang

{"title":"Restoring the FOXO1 geroprotective pathway via seno-resistant mesenchymal progenitor cells alleviates primate epididymal aging.","authors":"Huifen Lu, Linguo Cai, DongLiang Lv, Guoqiang Sun, Jinghui Lei, Taixin Ning, Zijuan Xin, Haoyan Huang, Ying Jing, Daoyuan Huang, Shuhui Sun, Shuai Ma, Weiqi Zhang, Fei Gao, Rui Chen, Yingying Qin, Weihong Song, Andy Peng Xiang, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Jing Qu, Si Wang","doi":"10.1093/procel/pwag020","DOIUrl":"https://doi.org/10.1093/procel/pwag020","url":null,"abstract":"Aging of the male reproductive system is characterized by declining fertility, with epididymal dysfunction being a critical yet poorly understood contributor. Through a multimodal analysis in non-human primates that integrated histology and transcriptomics, we delineated a coherent epididymal aging phenotype encompassing epithelial senescence, chronic inflammation, fibrosis, and functional decline. Single-nucleus transcriptomics revealed principal cells (PCs) as the predominant and most transcriptionally perturbed epithelial cell type. Within PCs, the longevity-associated transcription factor FOXO1 was markedly downregulated with age. Functional studies in human epididymal epithelial cells demonstrated that FOXO1 deficiency drives cellular senescence. Mechanistically, FOXO1 transcriptionally activates LHX1, and this axis is essential for counteracting senescence. Furthermore, intervention with senescence-resistant mesenchymal progenitor cells or their exosomes mitigated epididymal aging phenotypes and restored FOXO1 expression in vivo and in vitro. Our study establishes the FOXO1-LHX1 axis as a key protective pathway against primate epididymal aging, providing mechanistic insights and potential therapeutic targets for preserving male reproductive health.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147514349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular insights into Candida auris glycosylphosphatidylinositol transamidase. 耳念珠菌糖基磷脂酰肌醇转氨酶的分子研究。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2026-03-23 DOI: 10.1093/procel/pwag023

Xuyang Ding,Zhengkang Hua,Di Zhang,Jiameng Li,Yan Ke,Chu Qi,Ping Yang,Xinlin Hu,Junbo Liu,Mingjie Zhang,Min Zhang,Xiaotian Liu,Hongjun Yu

引用次数: 0

Molecular basis of high-torque transmission of the Vibrio polar flagellar motor. 极性鞭毛弧菌马达高扭矩传递的分子基础。

IF 12.8 1区生物学

Protein & Cell Pub Date : 2026-03-23 DOI: 10.1093/procel/pwag025

Ling Zhang, Jiaxing Tan, Xuemin Duan, Xiaofei Wang, Ting Wang, Keke Yuan, Michio Homma, Seiji Kojima, Yan Zhou, Yongqun Zhu

{"title":"Molecular basis of high-torque transmission of the Vibrio polar flagellar motor.","authors":"Ling Zhang, Jiaxing Tan, Xuemin Duan, Xiaofei Wang, Ting Wang, Keke Yuan, Michio Homma, Seiji Kojima, Yan Zhou, Yongqun Zhu","doi":"10.1093/procel/pwag025","DOIUrl":"https://doi.org/10.1093/procel/pwag025","url":null,"abstract":"The bacterial flagellar motor is a protein nanomachine that rotates the flagellum to facilitate bacterial motility. These motors exhibit structural diversity among species, enabling the transmission of varying torques to flagellar filaments to grant bacteria diverse swimming capabilities. Compared to peritrichous flagellar motors, polar flagellar motors are faster machines that transmit higher torque to drive high-speed motility in liquids and empower swimming in viscous environments. However, structural basis of high-torque transmission of the polar flagellar motors is still unclear. Here we present a cryo-electron microscopy structure of the polar flagellar motor in complex with the hook from Vibrio alginolyticus, comprising 295 subunits from 18 proteins. Compared to the peritrichous flagellar rod, this structure reveals an increased number of inter-subunit interactions in the rod of the polar flagellar motor. Nine phospholipid molecules insert into the interface between the export apparatus and the proximal rod. The LP ring contains more electrostatic charges on the inner surface and has fewer physical contacts with the rod, while the HT ring tightly binds to the outer surface of the LP ring. FlrP, a protein of previously unknown function, is identified as a new component of the polar flagellar motor, and extensively participates in the interactions of 15 FliF peptides of the MS ring with the rod. In contrast to that in the peritrichous flagellum, the hook in the polar flagellum has two different conformational states, L- and R-types. These findings provide unprecedented insights into structural adaptations of the bacterial polar flagellar motors for high-torque transmission.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147504689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kechun lin: a pioneer of biophysics explorations in China. 林克春：中国生物物理探索的先驱。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2026-03-18 DOI: 10.1093/procel/pwag015

Quanxiu Li,Cheng Zhen

引用次数: 0

Structural adaptation associated with signaling preference at the human GHRHR. 与人类GHRHR信号偏好相关的结构适应。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2026-03-18 DOI: 10.1093/procel/pwag016

Ming-Wei Wang,Kaini Hang,Yue Qiu,Xianyue Chen,Yanyan Chen,Shengtian Huang,Zhaotong Cong,Qingtong Zhou

引用次数: 0

Endosomes as Central Hubs of Interorganellar Communication and Cellular Homeostasis. 核内体作为细胞器间通讯和细胞稳态的中心枢纽。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2026-03-18 DOI: 10.1093/procel/pwag018

Yao Feng,Bo Zhou,Zihan Lu,Muyin Guo,Shaohui Zhang,Songlin Wang,Mo Chen

{"title":"Endosomes as Central Hubs of Interorganellar Communication and Cellular Homeostasis.","authors":"Yao Feng,Bo Zhou,Zihan Lu,Muyin Guo,Shaohui Zhang,Songlin Wang,Mo Chen","doi":"10.1093/procel/pwag018","DOIUrl":"https://doi.org/10.1093/procel/pwag018","url":null,"abstract":"Endocytosis mediates the internalization of extracellular cargo via vesicular trafficking, enabling targeted delivery to specific organelles and maintaining cellular homeostasis. Endosomes function as dynamic hubs that orchestrate intracellular communication. While they primarily internalize material from the cell surface, their role extends far beyond passive transport. Through continuous cycles of sorting, fusion, and fission, endosomes engage in extensive crosstalk with other organelles via three fundamental modes: vesicle-mediated cargo transport, membrane contact site-mediated non-vesicular exchange, and signaling and mechanical coupling. These interorganellar interactions enable the transfer of metabolites, lipids, and signals, positioning endosomes as central regulators of cellular homeostasis. While interest in these contacts is growing, a systematic understanding of their roles is still needed. This review explores the protein machinery involved, examines how endosome-organelle contacts coordinate transport and remodeling, and discusses their impact on homeostasis and disease when dysregulated. We underscore the importance of the endosomal communication network in adaptive responses and provide perspectives for targeting endosome-related pathologies.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":"33 1","pages":""},"PeriodicalIF":21.1,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147478964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RagC senses β-hydroxybutyrate abundancy to suppress mTORC1 and tumor growth. RagC感知β-羟基丁酸盐丰度，抑制mTORC1和肿瘤生长。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2026-03-18 DOI: 10.1093/procel/pwag017

Guoyan Wang,Qihang Hou,Yong Zhang,Qiuhui Duan,Jianxuan Gao,Jiangxin Wang,Jinrui Qiao,Yining Zheng,Xinjian Lei,Guowen Liu,Lin Lei,Tong Meng,Xiaojun Yang,Junhu Yao,Xinwei Li,Lu Deng

{"title":"RagC senses β-hydroxybutyrate abundancy to suppress mTORC1 and tumor growth.","authors":"Guoyan Wang,Qihang Hou,Yong Zhang,Qiuhui Duan,Jianxuan Gao,Jiangxin Wang,Jinrui Qiao,Yining Zheng,Xinjian Lei,Guowen Liu,Lin Lei,Tong Meng,Xiaojun Yang,Junhu Yao,Xinwei Li,Lu Deng","doi":"10.1093/procel/pwag017","DOIUrl":"https://doi.org/10.1093/procel/pwag017","url":null,"abstract":"The ketogenic diet (KD), an emerging nutritional intervention for cancer, reprograms cellular energy metabolism from glucose to ketone bodies, including acetoacetate (AcAc), acetone (Ac), and β-hydroxybutyrate (BHB). However, the mechanisms connecting ketone body signals sensing to tumor growth suppression remain elusive. Here, we show that RagC, a key component of mTORC1 pathway, senses BHB but not AcAc and Ac, to dictate tumor suppression. KD-derived BHB inhibits mTORC1 activity by promoting β-hydroxybutyrylation (Kbhb) of RagC at lysine 349 (K348 in mice). Mechanistically, RagC-K349bhb is dynamically catalyzed by p300 and erased by SIRT1, disrupting RagC interaction with Raptor/mTOR and blocking mTORC1 recruitment to lysosomes. Clinically, BHB-mediated RagC-K349bhb suppresses colorectal cancer (CRC) growth via mTORC1 inhibition in both RagC-K348R knockin mice and CRC patient-derived samples. Thus, we identify a BHB sensing mechanism by mTORC1 and highlight the potential role of RagC-K349bhb as a therapeutic target for BHB-based CRC treatment.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":"12 1","pages":""},"PeriodicalIF":21.1,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147478555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What we can learn from hibernators and other adapted animals about organ transplantation: mechanisms of cold resistance and enhanced regeneration. 我们可以从冬眠动物和其他适应器官移植的动物身上学到什么：抗寒和增强再生的机制。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2026-03-15 DOI: 10.1093/procel/pwag011

Hannah Esser,Robert J Porte,Luc J W van der Laan

引用次数: 0