Protein & Cell最新文献_第2页

Adenosine-to-inosine RNA editing in cancer: molecular mechanisms and downstream targets. 癌症中的腺苷转肌苷 RNA 编辑：分子机制和下游靶点。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-06-20 DOI: 10.1093/procel/pwae039

Hao Cheng, Jun Yu, Chi Chun Wong

引用次数: 0

Optimized derivation and culture system of human naïve pluripotent stem cells with enhanced DNA methylation status and genomic stability. 优化人类naïve多能干细胞的衍生和培养系统，提高DNA甲基化状态和基因组稳定性。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-06-20 DOI: 10.1093/procel/pwaf053

Yan Bi, Jindian Hu, Tao Wu, Zhaohui Ouyang, Tan Lin, Jiaxing Sun, Xinbao Zhang, Xiaoyu Xu, Hong Wang, Ke Wei, Shaorong Gao, Yixuan Wang

{"title":"Optimized derivation and culture system of human naïve pluripotent stem cells with enhanced DNA methylation status and genomic stability.","authors":"Yan Bi, Jindian Hu, Tao Wu, Zhaohui Ouyang, Tan Lin, Jiaxing Sun, Xinbao Zhang, Xiaoyu Xu, Hong Wang, Ke Wei, Shaorong Gao, Yixuan Wang","doi":"10.1093/procel/pwaf053","DOIUrl":"https://doi.org/10.1093/procel/pwaf053","url":null,"abstract":"Human naïve pluripotent stem cells (PSCs) hold great promise for embryonic development studies. Existing induction and culture strategies for these cells, heavily dependent on MEK inhibitors, lead to widespread DNA hypomethylation, aberrant imprinting loss, and genomic instability during extended culture. Here, employing high-content analysis alongside a bifluorescence reporter system indicative of human naïve pluripotency, we screened over 1,600 chemicals and identified 7 promising candidates. From these, we developed four optimized media-LAY, LADY, LUDY, and LKPY-that effectively induce and sustain PSCs in the naïve state. Notably, cells reset or cultured in these media, especially in the LAY system, demonstrate improved genome-wide DNA methylation status closely resembling that of pre-implantation counterparts, with partially restored imprinting and significantly enhanced genomic stability. Overall, our study contributes advancements to naïve pluripotency induction and long-term maintenance, providing insights for further applications of naïve PSCs.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA G-quadruplex (rG4) exacerbates cellular senescence by mediating ribosome pausing. RNA g -四重体（rG4）通过介导核糖体暂停而加剧细胞衰老。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-06-12 DOI: 10.1093/procel/pwaf047

Haoxian Zhou, Shu Wu, Bin Li, Rongjinlei Zhang, Ying Zou, Mibu Cao, Anhua Xu, Kewei Zheng, Qinghua Zhou, Jia Wang, Jinping Zheng, Jianhua Yang, Yuanlong Ge, Zhanyi Lin, Zhenyu Ju

{"title":"RNA G-quadruplex (rG4) exacerbates cellular senescence by mediating ribosome pausing.","authors":"Haoxian Zhou, Shu Wu, Bin Li, Rongjinlei Zhang, Ying Zou, Mibu Cao, Anhua Xu, Kewei Zheng, Qinghua Zhou, Jia Wang, Jinping Zheng, Jianhua Yang, Yuanlong Ge, Zhanyi Lin, Zhenyu Ju","doi":"10.1093/procel/pwaf047","DOIUrl":"https://doi.org/10.1093/procel/pwaf047","url":null,"abstract":"Loss of protein homeostasis is a hallmark of cellular senescence, and ribosome pausing plays a crucial role in the collapse of proteostasis. However, our understanding of ribosome pausing in senescent cells remains limited. In this study, we utilized ribosome profiling and G-quadruplex RNA immunoprecipitation sequencing techniques to explore the impact of RNA G-quadruplex (rG4) on the translation efficiency in senescent cells. Our results revealed a reduction in the translation efficiency of rG4-rich genes in senescent cells and demonstrated rG4 structures within coding sequence (CDS) can impede translation both in vivo and in vitro. Moreover, we observed a significant increase in the abundance of rG4 structures in senescent cells, and the stabilization of the rG4 structures further exacerbated cellular senescence. Mechanistically, the RNA helicase DHX9 functions as a key regulator of rG4 abundance, and its reduced expression in senescent cells contributing to increased ribosome pausing. Additionally, we also observed an increased abundance of rG4, an imbalance in protein homeostasis, and reduced DHX9 expression in aged mice. In summary, our findings reveal a novel biological role for rG4 and DHX9 in the regulation of translation and proteostasis, which may have implications for delaying cellular senescence and the aging process.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lamin C conserves DNA replication factors via phase separation during oxidative stress for DNA replication recovery. 层粘连蛋白C在氧化应激过程中通过相分离保护DNA复制因子，促进DNA复制恢复。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-06-11 DOI: 10.1093/procel/pwaf016

Mingkang Jia, Gan Zhao, Mengjie Sun, Xiangyang Wang, He Ren, Guangwei Xin, Qing Jiang, Chuanmao Zhang

引用次数: 0

Spatiotemporal characterization of disease-associated neurons in the entorhinal cortex-hippocampal circuit during AD progression. 阿尔茨海默病进展期间内嗅皮层-海马回路中疾病相关神经元的时空特征

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-06-10 DOI: 10.1093/procel/pwaf042

Yuting Ma, Juan Zhang, Hankui Liu, Dingfeng Li, Sicheng Guo, Jialuo Han, Lei Wang, Shaojun Yu, Xi Su, Yongchang Gao, Xiumei Lin, A San, Yushan Peng, Guibo Li, Hui Jiang, Wei Wang, Huanming Yang, Jian Wang, Shida Zhu, Lijian Zhao, Jianguo Zhang, Qiang Liu

{"title":"Spatiotemporal characterization of disease-associated neurons in the entorhinal cortex-hippocampal circuit during AD progression.","authors":"Yuting Ma, Juan Zhang, Hankui Liu, Dingfeng Li, Sicheng Guo, Jialuo Han, Lei Wang, Shaojun Yu, Xi Su, Yongchang Gao, Xiumei Lin, A San, Yushan Peng, Guibo Li, Hui Jiang, Wei Wang, Huanming Yang, Jian Wang, Shida Zhu, Lijian Zhao, Jianguo Zhang, Qiang Liu","doi":"10.1093/procel/pwaf042","DOIUrl":"https://doi.org/10.1093/procel/pwaf042","url":null,"abstract":"The entorhinal cortex (EC)-hippocampal (HPC) circuit is particularly vulnerable to Alzheimer's disease (AD) pathology, yet the underlying molecular mechanisms remain unclear. By employing the high-depth sequencing strategy Smart-seq2, we tracked gene expression changes across various neuron types within this circuit at different stages of AD pathology. We observed a decrease in the extent of gene expression changes in AD versus wild-type (WT) mice as the disease advanced. Functionally, we demonstrate that both mitochondrial and ribosomal pathways were increasingly activated, while neuronal pathways were inhibited with AD progression. Our findings indicate that the reduction of EC-stellate cells disrupts Meg3-mediated energy metabolism, contributing to energy dysfunction in AD. Additionally, we identified GFAP-positive neurons as a distinct population of disease-associated neurons, exhibiting a loss of neuronal-like characteristics, alongside the emergence of glia- and stem-like features. The number of GFAP-positive neurons increased with AD progression, a trend consistently observed in both AD model mice and AD patients. In summary, this study identifies and characterizes GFAP-positive neurons as a novel subtype of disease-associated neurons in AD pathology, providing insights into their potential role in disease progression.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Engineered extracellular vesicles enable high-efficient delivery of intracellular therapeutic proteins. 修正：工程细胞外囊泡能够高效地递送细胞内治疗蛋白。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-06-06 DOI: 10.1093/procel/pwaf037

引用次数: 0

Bardoxolone methyl blocks the efflux of Zn2+ by targeting hZnT1 to inhibit the proliferation and metastasis of cervical cancer. 甲基巴多洛酮通过靶向hZnT1阻断Zn2+的外排，抑制宫颈癌的增殖转移。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-06-05 DOI: 10.1093/procel/pwaf044

Yaxin Wang, Qinqin Liang, Shengjian Liang, Yuanyue Shan, Sai Shi, Xiaoyu Zhou, Ziyu Wang, Zhili Xu, Duanqing Pei, Mingfeng Zhang, Zhiyong Lou, Binghong Xu, Sheng Ye

引用次数: 0

Structure, identification and characterization of the RibD-enolase complex in Francisella. 弗朗西斯菌ribd -烯醇化酶复合物的结构、鉴定和表征。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-06-05 DOI: 10.1093/procel/pwaf045

Xiaoyu Liu, Daniel L Clemens, Bai-Yu Lee, Roman Aguirre, Marcus A Horwitz, Z Hong Zhou

引用次数: 0

Correction to: Genome-wide CRISPR screen identifies synthetic lethality between DOCK1 inhibition and metformin in liver cancer. 更正：全基因组CRISPR筛选鉴定了肝癌中DOCK1抑制和二甲双胍之间的合成致死性。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-06-04 DOI: 10.1093/procel/pwaf036

引用次数: 0

Advances in gene and cellular therapeutic approaches for Huntington's disease. 亨廷顿氏症基因和细胞治疗方法的进展。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-05-28 DOI: 10.1093/procel/pwae042

Xuejiao Piao, Dan Li, Hui Liu, Qing Guo, Yang Yu

{"title":"Advances in gene and cellular therapeutic approaches for Huntington's disease.","authors":"Xuejiao Piao, Dan Li, Hui Liu, Qing Guo, Yang Yu","doi":"10.1093/procel/pwae042","DOIUrl":"10.1093/procel/pwae042","url":null,"abstract":"Huntington's disease (HD) is an inherited neurodegenerative disorder caused by the abnormal expansion of CAG trinucleotide repeats in the Huntingtin gene (HTT) located on chromosome 4. It is transmitted in an autosomal dominant manner and is characterized by motor dysfunction, cognitive decline, and emotional disturbances. To date, there are no curative treatments for HD have been developed; current therapeutic approaches focus on symptom relief and comprehensive care through coordinated pharmacological and nonpharmacological methods to manage the diverse phenotypes of the disease. International clinical guidelines for the treatment of HD are continually being revised in an effort to enhance care within a multidisciplinary framework. Additionally, innovative gene and cell therapy strategies are being actively researched and developed to address the complexities of the disorder and improve treatment outcomes. This review endeavours to elucidate the current and emerging gene and cell therapy strategies for HD, offering a detailed insight into the complexities of the disorder and looking forward to future treatment paradigms. Considering the complexity of the underlying mechanisms driving HD, a synergistic treatment strategy that integrates various factors-such as distinct cell types, epigenetic patterns, genetic components, and methods to improve the cerebral microenvironment-may significantly enhance therapeutic outcomes. In the future, we eagerly anticipate ongoing innovations in interdisciplinary research that will bring profound advancements and refinements in the treatment of HD.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":"307-337"},"PeriodicalIF":13.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141910099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0