Protein & Cell最新文献_第6页

Cryo-EM structures of Nipah virus polymerase complex reveal highly varied interactions between L and P proteins among paramyxoviruses. 尼帕病毒聚合酶复合物的低温电镜结构揭示了副粘病毒之间L和P蛋白之间高度不同的相互作用。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-18 DOI: 10.1093/procel/pwaf014

Lu Xue, Tiancai Chang, Jiacheng Gui, Zimu Li, Heyu Zhao, Bingqian Zou, Junnan Lu, Mei Li, Xin Wen, Shenghua Gao, Peng Zhan, Lijun Rong, Liqiang Feng, Peng Gong, Jun He, Xinwen Chen, Xiaoli Xiong

{"title":"Cryo-EM structures of Nipah virus polymerase complex reveal highly varied interactions between L and P proteins among paramyxoviruses.","authors":"Lu Xue, Tiancai Chang, Jiacheng Gui, Zimu Li, Heyu Zhao, Bingqian Zou, Junnan Lu, Mei Li, Xin Wen, Shenghua Gao, Peng Zhan, Lijun Rong, Liqiang Feng, Peng Gong, Jun He, Xinwen Chen, Xiaoli Xiong","doi":"10.1093/procel/pwaf014","DOIUrl":"https://doi.org/10.1093/procel/pwaf014","url":null,"abstract":"Nipah virus (NiV) and related viruses form a distinct henipavirus genus within the Paramyxoviridae family. NiV continues to spillover into the humans causing deadly outbreaks with increasing human-bat interaction. NiV encodes the large protein (L) and phosphoprotein (P) to form the viral RNA polymerase machinery. Their sequences show limited homologies to those of non-henipavirus paramyxoviruses. We report two cryo-electron microscopy (cryo-EM) structures of the Nipah virus (NiV) polymerase L-P complex, expressed and purified in either its full-length or truncated form. The structures resolve the RNA-dependent RNA polymerase (RdRp) and polyribonucleotidyl transferase (PRNTase) domains of the L protein, as well as a tetrameric P protein bundle bound to the L-RdRp. L-protein C-terminal regions are unresolved, indicating flexibility. Two PRNTase domain zinc-binding sites, conserved in most Mononegavirales, are confirmed essential for NiV polymerase activity. The structures further reveal anchoring of the P protein bundle and P protein X domain (XD) linkers on L, via an interaction pattern distinct among Paramyxoviridae. These interactions facilitate binding of a P protein XD linker in the nucleotide entry channel and distinct positioning of other XD linkers. We show that the disruption of the L-P interactions reduces NiV polymerase activity. The reported structures should facilitate rational antiviral-drug discovery and provide a guide for the functional study of NiV polymerase.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143450206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral activity of lipoxygenase against severe fever with thrombocytopenia syndrome virus. 脂氧合酶对严重发热伴血小板减少综合征病毒的抗病毒活性

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-01 DOI: 10.1093/procel/pwae061

Shuang Li, Xiaojie Zheng, Yunfa Zhang, Lingyu Zhang, Tong Yang, Hao Li, Caiyu Zhou, Xiao-Ai Zhang, Li-Zeng Gao, Wei Liu

引用次数: 0

RADICAL: a rationally designed ion channel activated by ligand for chemogenetics. RADICAL：通过配体激活的合理设计的离子通道，用于化学遗传学。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-01 DOI: 10.1093/procel/pwae048

Heng Zhang, Zhiwei Zheng, Xiaoying Chen, Lizhen Xu, Chen Guo, Jiawei Wang, Yihui Cui, Fan Yang

引用次数: 0

Lcn2 secreted by macrophages through NLRP3 signaling pathway induced severe pneumonia. 巨噬细胞通过 NLRP3 信号通路分泌的 Lcn2 可诱发重症肺炎。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-01 DOI: 10.1093/procel/pwae045

Mingya Liu, Feifei Qi, Jue Wang, Fengdi Li, Qi Lv, Ran Deng, Xujian Liang, Shasha Zhou, Pin Yu, Yanfeng Xu, Yaqing Zhang, Yiwei Yan, Ming Liu, Shuyue Li, Guocui Mou, Linlin Bao

引用次数: 0

Alzheimer's disease: insights into pathology, molecular mechanisms, and therapy. 阿尔茨海默病：对病理学、分子机制和治疗的见解。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-01 DOI: 10.1093/procel/pwae026

Qiuyang Zheng, Xin Wang

引用次数: 0

Cas7 meets Cas14: a strategic partnership in the type VII CRISPR-Cas. Cas7与Cas14: VII型CRISPR-Cas的战略合作伙伴关系

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-02-01 DOI: 10.1093/procel/pwae056

Yao Liu, Senfeng Zhang, Chunyi Hu

引用次数: 0

An upgraded nuclease prime editor platform enables high-efficiency singled or multiplexed knock-in/knockout of genes in mouse and sheep zygotes. 升级的核酸酶引物编辑平台能够高效地在小鼠和绵羊受精卵中进行单敲或多重敲入/敲除基因。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2025-01-20 DOI: 10.1093/procel/pwaf006

Weijia Mao,Pei Wang,Lei Zhou,Dongxu Li,Xiangyang Li,Xin Lou,Xingxu Huang,Feng Wang,Yanli Zhang,Jianghuai Liu,Yongjie Wan

引用次数: 0

FOXO3-engineered human mesenchymal stem cells efficiently enhance post-ischemic stroke functional rehabilitation. foxo3工程人间充质干细胞有效促进缺血性脑卒中后功能康复。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2025-01-17 DOI: 10.1093/procel/pwaf004

Fangshuo Zheng,Jinghui Lei,Zan He,Taixin Ning,Shuhui Sun,Yusheng Cai,Qian Zhao,Shuai Ma,Weiqi Zhang,Jing Qu,Guang-Hui Liu,Si Wang

引用次数: 0

The Icarian flight of antibody-drug conjugates: target selection amidst complexity and tackling adverse impacts. 抗体-药物偶联物的伊卡利亚飞行：复杂中的靶标选择和处理不利影响。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2025-01-15 DOI: 10.1093/procel/pwaf002

Han Liu,Hongye Zeng,Xiaojing Qin,Wenjing Ning,Lin Xu,Shiting Yang,Xue Liu,Wenxin Luo,Ningshao Xia

{"title":"The Icarian flight of antibody-drug conjugates: target selection amidst complexity and tackling adverse impacts.","authors":"Han Liu,Hongye Zeng,Xiaojing Qin,Wenjing Ning,Lin Xu,Shiting Yang,Xue Liu,Wenxin Luo,Ningshao Xia","doi":"10.1093/procel/pwaf002","DOIUrl":"https://doi.org/10.1093/procel/pwaf002","url":null,"abstract":"Antibody-drug conjugates (ADCs) represent a promising class of targeted cancer therapeutics that combine the specificity of monoclonal antibodies with the potency of cytotoxic payloads. Despite their therapeutic potential, the use of ADCs faces significant challenges, including off/on-target toxicity and resistance development. This review examines the current landscape of ADC development, focusing on the critical aspects of target selection and antibody engineering. We discuss strategies to increase ADC efficacy and safety, including multitarget approaches, pH-dependent antibodies, and masked peptide technologies. The importance of comprehensive antigen expression profiling in both tumor and normal tissues is emphasized, highlighting the role of advanced technologies, such as single-cell sequencing and artificial intelligence (AI), in optimizing target selection. Furthermore, we explore combination therapies and innovations in linker‒payload chemistry, which may provide approaches for expanding the therapeutic window of ADCs. These advances pave the way for the development of more precise and effective cancer treatments, potentially extending ADC applications beyond oncology.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":"127 1","pages":""},"PeriodicalIF":21.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin landscape alteration uncovers multiple transcriptional circuits during memory CD8+ T cell differentiation. 染色质景观改变揭示了记忆 CD8+ T 细胞分化过程中的多个转录回路。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2025-01-13 DOI: 10.1093/procel/pwaf003

Qiao Liu, Wei Dong, Rong Liu, Luming Xu, Ling Ran, Ziying Xie, Shun Lei, Xingxing Su, Zhengliang Yue, Dan Xiong, Lisha Wang, Shuqiong Wen, Yan Zhang, Jianjun Hu, Chenxi Qin, Yongchang Chen, Bo Zhu, Xiangyu Chen, Xia Wu, Lifan Xu, Qizhao Huang, Yingjiao Cao, Lilin Ye, Zhonghui Tang

{"title":"Chromatin landscape alteration uncovers multiple transcriptional circuits during memory CD8+ T cell differentiation.","authors":"Qiao Liu, Wei Dong, Rong Liu, Luming Xu, Ling Ran, Ziying Xie, Shun Lei, Xingxing Su, Zhengliang Yue, Dan Xiong, Lisha Wang, Shuqiong Wen, Yan Zhang, Jianjun Hu, Chenxi Qin, Yongchang Chen, Bo Zhu, Xiangyu Chen, Xia Wu, Lifan Xu, Qizhao Huang, Yingjiao Cao, Lilin Ye, Zhonghui Tang","doi":"10.1093/procel/pwaf003","DOIUrl":"https://doi.org/10.1093/procel/pwaf003","url":null,"abstract":"Extensive epigenetic reprogramming involves in memory CD8+ T-cell differentiation. The elaborate epigenetic rewiring underlying the heterogeneous functional states of CD8+ T cells remains hidden. Here, we profile single-cell chromatin accessibility and map enhancer-promoter interactomes to characterize the differentiation trajectory of memory CD8+ T cells. We reveal that under distinct epigenetic regulations, the early activated CD8+ T cells divergently originated for short-lived effector and memory precursor effector cells. We also uncover a defined epigenetic rewiring leading to the conversion from effector memory to central memory cells during memory formation. Additionally, we illustrate chromatin regulatory mechanisms underlying long-lasting versus transient transcription regulation during memory differentiation. Finally, we confirm the essential roles of Sox4 and Nrf2 in developing memory precursor effector and effector memory cells, respectively, and validate cell state-specific enhancers in regulating Il7r using CRISPR-Cas9. Our data pave the way for understanding the mechanism underlying epigenetic memory formation in CD8+ T-cell differentiation.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0