Protein & Cell最新文献_第6页

Microbiome, metabolome and transcriptome analyses in esophageal squamous cell carcinoma: Insights into immune modulation by F. nucleatum. 食管鳞状细胞癌的微生物组、代谢组和转录组分析：洞察核酸酵母菌的免疫调节作用。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-10-29 DOI: 10.1093/procel/pwae063

Xue Zhang, Jing Han, Yudong Wang, Li Feng, Zhisong Fan, Yu Su, Wenya Song, Lan Wang, Long Wang, Hui Jin, Jiayin Liu, Dan Li, Guiying Li, Yan Liu, Jing Zuo, Zhiyu Ni

引用次数: 0

JMJD1C forms condensates to facilitate a RUNX1-dependent gene expression program shared by multiple types of AML cells. JMJD1C 形成凝聚体，促进多种类型急性髓细胞白血病细胞共有的 RUNX1 依赖性基因表达程序。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-10-25 DOI: 10.1093/procel/pwae059

Qian Chen,Saisai Wang,Juqing Zhang,Min Xie,Bin Lu,Jie He,Zhuoran Zhen,Jing Li,Jiajun Zhu,Rong Li,Pilong Li,Haifeng Wang,Christopher Vakoc,Robert G Roeder,Mo Chen

{"title":"JMJD1C forms condensates to facilitate a RUNX1-dependent gene expression program shared by multiple types of AML cells.","authors":"Qian Chen,Saisai Wang,Juqing Zhang,Min Xie,Bin Lu,Jie He,Zhuoran Zhen,Jing Li,Jiajun Zhu,Rong Li,Pilong Li,Haifeng Wang,Christopher Vakoc,Robert G Roeder,Mo Chen","doi":"10.1093/procel/pwae059","DOIUrl":"https://doi.org/10.1093/procel/pwae059","url":null,"abstract":"JMJD1C, a member of the lysine demethylase 3 (KDM3) family, is universally required for the survival of several types of acute myeloid leukemia (AML) cells with different genetic mutations, representing a therapeutic opportunity with broad application. Yet how JMJD1C regulates the leukemic programs of various AML cells is largely unexplored. Here we show that JMJD1C interacts with the master hematopoietic transcription factor RUNX1, which thereby recruits JMJD1C to the genome to facilitate a RUNX1-driven transcriptional program that supports leukemic cell survival. The underlying mechanism hinges on the long N-terminal disordered region of JMJD1C, which harbors two inseparable abilities: condensate formation and direct interaction with RUNX1. This dual capability of JMJD1C may influence enhancer-promoter contacts crucial for the expression of key leukemic genes regulated by RUNX1. Our findings demonstrate a previously unappreciated role for the non-catalytic function of JMJD1C in transcriptional regulation, underlying a mechanism shared by different types of leukemias.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":"96 1","pages":""},"PeriodicalIF":21.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142490565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress granules and organelles: Coordinating cellular responses in health and disease. 应激颗粒和细胞器：协调健康和疾病中的细胞反应

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-10-23 DOI: 10.1093/procel/pwae057

Ying Liu,Yin Li,Peipei Zhang

引用次数: 0

Quantitative real-time in vitro transcription assay (QRIVTA) for transcriptional regulation studies. 用于转录调控研究的定量实时体外转录测定（QRIVTA）。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-10-10 DOI: 10.1093/procel/pwae054

Fan Liu,Jing Xu,Xinli Hu,Bo Duan,Bin Xia

引用次数: 0

Skin organoid transplantation promotes tissue repair with scarless in frostbite. 皮肤类器官移植可促进冻伤组织的无疤痕修复。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-10-04 DOI: 10.1093/procel/pwae055

Wenwen Wang, Pu Liu, Wendi Zhu, Tianwei Li, Ying Wang, Yujie Wang, Jun Li, Jie Ma, Ling Leng

{"title":"Skin organoid transplantation promotes tissue repair with scarless in frostbite.","authors":"Wenwen Wang, Pu Liu, Wendi Zhu, Tianwei Li, Ying Wang, Yujie Wang, Jun Li, Jie Ma, Ling Leng","doi":"10.1093/procel/pwae055","DOIUrl":"https://doi.org/10.1093/procel/pwae055","url":null,"abstract":"Frostbite is the most common cold injury and is caused by both immediate cold-induced cell death and the gradual development of localized inflammation and tissue ischemia. Delayed healing of frostbite often leads to scar formation, which not only causes psychological distress but also tends to result in the development of secondary malignant tumors. Therefore, a rapid healing method for frostbite wounds is urgently needed. Herein, we used a mouse skin model of frostbite injury to evaluate the recovery process after frostbite. Moreover, single-cell transcriptomics was used to determine the patterns of changes in monocytes, macrophages, epidermal cells and fibroblasts during frostbite. Most importantly, human-induced pluripotent stem cell (hiPSC) -derived skin organoids combining with gelatin-hydrogel were constructed for the treatment of frostbite. The results showed that skin organoid treatment significantly accelerated wound healing by reducing early inflammation after frostbite and increasing the proportions of epidermal stem cells. Moreover, in the later stage of wound healing, skin organoids reduced the overall proportions of fibroblasts, significantly reduced fibroblast-to-myofibroblast transition by regulating the integrin α5β1-FAK pathway, and remodeled the extracellular matrix (ECM) through degradation and reassembly mechanisms, facilitating the restoration of physiological ECM and reducing the abundance of ECM associated with abnormal scar formation. These results highlight the potential application of organoids for promoting the reversal of frostbite-related injury and the recovery of skin functions. This study provides a new therapeutic alternative for patients suffering from disfigurement and skin dysfunction caused by frostbite.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":13.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered extracellular vesicles enable high-efficient delivery of intracellular therapeutic proteins. 经过设计的细胞外囊泡能够高效输送细胞内治疗蛋白。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-10-01 DOI: 10.1093/procel/pwae015

Ding Ma, An Xie, Jiahui Lv, Xiaolin Min, Xinye Zhang, Qian Zhou, Daxing Gao, Enyu Wang, Lei Gao, Linzhao Cheng, Senquan Liu

{"title":"Engineered extracellular vesicles enable high-efficient delivery of intracellular therapeutic proteins.","authors":"Ding Ma, An Xie, Jiahui Lv, Xiaolin Min, Xinye Zhang, Qian Zhou, Daxing Gao, Enyu Wang, Lei Gao, Linzhao Cheng, Senquan Liu","doi":"10.1093/procel/pwae015","DOIUrl":"10.1093/procel/pwae015","url":null,"abstract":"Developing an intracellular delivery system is of key importance in the expansion of protein-based therapeutics acting on cytosolic or nuclear targets. Recently, extracellular vesicles (EVs) have been exploited as next-generation delivery modalities due to their natural role in intercellular communication and biocompatibility. However, fusion of protein of interest to a scaffold represents a widely used strategy for cargo enrichment in EVs, which could compromise the stability and functionality of cargo. Herein, we report intracellular delivery via EV-based approach (IDEA) that efficiently packages and delivers native proteins both in vitro and in vivo without the use of a scaffold. As a proof-of-concept, we applied the IDEA to deliver cyclic GMP-AMP synthase (cGAS), an innate immune sensor. The results showed that cGAS-carrying EVs activated interferon signaling and elicited enhanced antitumor immunity in multiple syngeneic tumor models. Combining cGAS EVs with immune checkpoint inhibition further synergistically boosted antitumor efficacy in vivo. Mechanistically, scRNA-seq demonstrated that cGAS EVs mediated significant remodeling of intratumoral microenvironment, revealing a pivotal role of infiltrating neutrophils in the antitumor immune milieu. Collectively, IDEA, as a universal and facile strategy, can be applied to expand and advance the development of protein-based therapeutics.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":"724-743"},"PeriodicalIF":13.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140190063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-exome sequencing identifies ECPAS as a novel potentially pathogenic gene in multiple hereditary families with nonsyndromic orofacial cleft. 全基因组测序发现，ECPAS 是多个非综合征口面裂遗传性家族中的一个新的潜在致病基因。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-10-01 DOI: 10.1093/procel/pwae021

Huaxiang Zhao, Wenjie Zhong, Wenbin Huang, Guozhu Ning, Jieni Zhang, Mengqi Zhang, Peiqi Meng, Yunfan Zhang, Qian Zhang, Hongping Zhu, Gulibaha Maimaitili, Yi Ding, Weiran Li, Wei Liang, Zhibo Zhou, Qiang Wang, Feng Chen, Jiuxiang Lin

引用次数: 0

CARM1 drives triple-negative breast cancer progression by coordinating with HIF1A. CARM1 通过与 HIF1A 相互配合，推动三阴性乳腺癌的进展。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-10-01 DOI: 10.1093/procel/pwae010

Dandan Feng, Jie Gao, Ruiqiong Liu, Wei Liu, Tianyang Gao, Yunkai Yang, Die Zhang, Tianshu Yang, Xin Yin, Hefen Yu, Wei Huang, Yan Wang

{"title":"CARM1 drives triple-negative breast cancer progression by coordinating with HIF1A.","authors":"Dandan Feng, Jie Gao, Ruiqiong Liu, Wei Liu, Tianyang Gao, Yunkai Yang, Die Zhang, Tianshu Yang, Xin Yin, Hefen Yu, Wei Huang, Yan Wang","doi":"10.1093/procel/pwae010","DOIUrl":"10.1093/procel/pwae010","url":null,"abstract":"Coactivator-associated arginine methyltransferase 1 (CARM1) promotes the development and metastasis of estrogen receptor alpha (ERα)-positive breast cancer. The function of CARM1 in triple-negative breast cancer (TNBC) is still unclear and requires further exploration. Here, we report that CARM1 promotes proliferation, epithelial-mesenchymal transition, and stemness in TNBC. CARM1 is upregulated in multiple cancers and its expression correlates with breast cancer progression. Genome-wide analysis of CARM1 showed that CARM1 is recruited by hypoxia-inducible factor-1 subunit alpha (HIF1A) and occupy the promoters of CDK4, Cyclin D1, β-Catenin, HIF1A, MALAT1, and SIX1 critically involved in cell cycle, HIF-1 signaling pathway, Wnt signaling pathway, VEGF signaling pathway, thereby modulating the proliferation and invasion of TNBC cells. We demonstrated that CARM1 is physically associated with and directly interacts with HIF1A. Moreover, we found that ellagic acid, an inhibitor of CARM1, can suppress the proliferation and invasion of TNBC by directly inhibiting CDK4 expression. Our research has determined the molecular basis of CARM1 carcinogenesis in TNBC and its effective natural inhibitor, which may provide new ideas and drugs for cancer therapy.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":"744-765"},"PeriodicalIF":13.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody. 细胞运动需要 NudCL2，它能在中体通过 Hsp90 稳定 RCC2。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-10-01 DOI: 10.1093/procel/pwae025

Xiaoyang Xu, Yuliang Huang, Feng Yang, Xiaoxia Sun, Rijin Lin, Jiaxing Feng, Mingyang Yang, Jiaqi Shao, Xiaoqi Liu, Tianhua Zhou, Shanshan Xie, Yuehong Yang

{"title":"NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody.","authors":"Xiaoyang Xu, Yuliang Huang, Feng Yang, Xiaoxia Sun, Rijin Lin, Jiaxing Feng, Mingyang Yang, Jiaqi Shao, Xiaoqi Liu, Tianhua Zhou, Shanshan Xie, Yuehong Yang","doi":"10.1093/procel/pwae025","DOIUrl":"10.1093/procel/pwae025","url":null,"abstract":"Cytokinesis is required for faithful division of cytoplasmic components and duplicated nuclei into two daughter cells. Midbody, a protein-dense organelle that forms at the intercellular bridge, is indispensable for successful cytokinesis. However, the regulatory mechanism of cytokinesis at the midbody still remains elusive. Here, we unveil a critical role for NudC-like protein 2 (NudCL2), a co-chaperone of heat shock protein 90 (Hsp90), in cytokinesis regulation by stabilizing regulator of chromosome condensation 2 (RCC2) at the midbody in mammalian cells. NudCL2 localizes at the midbody, and its downregulation results in cytokinesis failure, multinucleation, and midbody disorganization. Using iTRAQ-based quantitative proteomic analysis, we find that RCC2 levels are decreased in NudCL2 knockout (KO) cells. Moreover, Hsp90 forms a complex with NudCL2 to stabilize RCC2, which is essential for cytokinesis. RCC2 depletion mirrors phenotypes observed in NudCL2-downregulated cells. Importantly, ectopic expression of RCC2 rescues the cytokinesis defects induced by NudCL2 deletion, but not vice versa. Together, our data reveal the significance of the NudCL2/Hsp90/RCC2 pathway in cytokinesis at the midbody.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":"766-782"},"PeriodicalIF":13.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SMURF1: A promising target for colon cancer therapy. SMURF1：有望成为结肠癌治疗靶点

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-09-27 DOI: 10.1093/procel/pwae053

Xiufang Xiong,Yongchao Zhao,Yi Sun

引用次数: 0