IF 2.5 3区生物学

Protist Pub Date : 2022-12-01 DOI: 10.1016/j.protis.2022.125915

John G. Day , Katharine H. Childs , Glyn N. Stacey

{"title":"Implications of a Catastrophic Refrigeration Failure on the Viability of Cryogenically Stored Samples","authors":"John G. Day , Katharine H. Childs , Glyn N. Stacey","doi":"10.1016/j.protis.2022.125915","DOIUrl":"10.1016/j.protis.2022.125915","url":null,"abstract":"<div>Cryopreservation, the use of very low temperatures to preserve structurally intact living cells and tissues, is a key underpinning technology for life science research and medicine. It is employed to ensure the stability of critical biological resources including viruses, bacteria, protists, animal cell cultures, plants, reproductive materials and embryos. Fundamental to ensuring this stability is assuring stability of cryogenic storage temperatures. Here we report the occurrence of a failure in refrigeration in a cryostat holding > 600 strains of cyanobacteria and eukaryotic microalgae. A strategic approach was adopted to assess viability across a cross-section of the biodiversity held, both immediately after the potentially damaging temperature shift and 10 years later, on subsequent cryostorage in liquid-phase nitrogen (∼−196 °C). Furthermore, the event was replicated experimentally and the effects on the viability of cryo-tolerant and cryo-sensitive strains monitored. Our results have significant implications to all users of this storage method and parallels have been drawn with the ongoing development in other fields and in particular, human cell therapy. Based on our practical experience we have made a series of generic recommendations for emergency, remedial and ongoing preventative actions.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6","pages":"Article 125915"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461022000608/pdfft?md5=068d903fd66f1e23dca35f6275ede893&pid=1-s2.0-S1434461022000608-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10504919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Significance of the Nudiform and Tectiform Modes of Silica Deposition, Lorica Assembly and Cell Division in Choanoflagellates as Exemplified by Stephanoeca diplocostata and Enibas spp. 以Stephanoeca diplocostata和enspp为例的毛鞭毛虫裸形和隐形二氧化硅沉积、Lorica组装和细胞分裂模式的意义

IF 2.5 3区生物学

Protist Pub Date : 2022-12-01 DOI: 10.1016/j.protis.2022.125923

Barry S.C. Leadbeater , Martin Carr

{"title":"Significance of the Nudiform and Tectiform Modes of Silica Deposition, Lorica Assembly and Cell Division in Choanoflagellates as Exemplified by Stephanoeca diplocostata and Enibas spp.","authors":"Barry S.C. Leadbeater , Martin Carr","doi":"10.1016/j.protis.2022.125923","DOIUrl":"10.1016/j.protis.2022.125923","url":null,"abstract":"<div>The deposition of silicified costal strips and lorica assembly in choanoflagellates is precisely linked to the cell cycle. A minority of species undergo nudiform division whereby a loricate cell divides to produce a naked daughter cell that deposits a set of costal strips and then assembles a lorica. Most species undergo tectiform division whereby a parent loricate cell produces a set of costal strips, divides and passes on the stored strips to a daughter cell that immediately assembles a lorica. Many phylogenetic analyses recover nudiform and tectiform species as sister-clades giving the impression that they are distinct evolutionary lineages. However, the tectiform species Stephanoeca diplocostata is capable of undergoing nudiform division and depositing costal strips and assembling a lorica with certain modifications in a nudiform manner. The recent discovery of a new genus, Enibas, comprising species with Stephanoeca-like loricae that undergo nudiform cell division and on phylogenetic analysis occur as a sister group to other nudiform species has drawn attention to whether there are also unique features in lorica construction. A range of Enibas loricae is illustrated and it appears that there are unique features which might be interpreted as being derived from a Stephanoeca-like ancestor.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6","pages":"Article 125923"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461022000682/pdfft?md5=e618c0add9219f4ea2825c95dc2677d6&pid=1-s2.0-S1434461022000682-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10448541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What Drives the Assembly of Plant-associated Protist Microbiomes? Investigating the Effects of Crop Species, Soil Type and Bacterial Microbiomes 是什么驱动植物相关原生微生物组的组装?研究作物种类、土壤类型和细菌微生物组的影响

IF 2.5 3区生物学

Protist Pub Date : 2022-12-01 DOI: 10.1016/j.protis.2022.125913

Kenneth Dumack , Kai Feng , Sebastian Flues , Melanie Sapp , Susanne Schreiter , Rita Grosch , Laura E. Rose , Ye Deng , Kornelia Smalla , Michael Bonkowski

{"title":"What Drives the Assembly of Plant-associated Protist Microbiomes? Investigating the Effects of Crop Species, Soil Type and Bacterial Microbiomes","authors":"Kenneth Dumack , Kai Feng , Sebastian Flues , Melanie Sapp , Susanne Schreiter , Rita Grosch , Laura E. Rose , Ye Deng , Kornelia Smalla , Michael Bonkowski","doi":"10.1016/j.protis.2022.125913","DOIUrl":"10.1016/j.protis.2022.125913","url":null,"abstract":"<div>In a field experiment we investigated the influence of the environmental filters soil type (i.e. three contrasting soils) and plant species (i.e. lettuce and potato) identity on rhizosphere community assembly of Cercozoa, a dominant group of mostly bacterivorous soil protists. Plant species (14%) and rhizosphere origin (vs bulk soil) with 13%, together explained four times more variation in cercozoan beta diversity than the three soil types (7% explained variation). Our results clearly confirm the existence of plant species-specific protist communities. Network analyses of bacteria-Cercozoa rhizosphere communities identified scale-free small world topologies, indicating mechanisms of self-organization. While the assembly of rhizosphere bacterial communities is bottom-up controlled through the resource supply from root (secondary) metabolites, our results support the hypothesis that the net effect may depend on the strength of top-down control by protist grazers. Since grazing of protists has a strong impact on the composition and functioning of bacteria communities, protists expand the repertoire of plant genes by functional traits, and should be considered as ‘protist microbiomes’ in analogy to ‘bacterial microbiomes’.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6","pages":"Article 125913"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S143446102200058X/pdfft?md5=a3ba5252fc8db726f44e5af7a07ce137&pid=1-s2.0-S143446102200058X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10813684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-evaluating Loricate Choanoflagellate Phylogenetics: Molecular Evidence Points to the Paraphyly of Tectiform Species 重新评价扇尾鞭毛虫的系统发育:分子证据指向隐形类的类群

IF 2.5 3区生物学

Protist Pub Date : 2022-12-01 DOI: 10.1016/j.protis.2022.125924

Martin Carr , Barry S.C. Leadbeater

{"title":"Re-evaluating Loricate Choanoflagellate Phylogenetics: Molecular Evidence Points to the Paraphyly of Tectiform Species","authors":"Martin Carr , Barry S.C. Leadbeater","doi":"10.1016/j.protis.2022.125924","DOIUrl":"10.1016/j.protis.2022.125924","url":null,"abstract":"<div>Lorica-bearing choanoflagellates belong to the order Acanthoecida, a taxon which has been consistently recovered as monophyletic in molecular phylogenies. Based upon differences in lorica development and morphology, as well as the presence or absence of a motile dispersal stage, species are labelled as either nudiform or tectiform. Whilst Acanthoecida is robustly resolved in molecular phylogenies, the placement of the root of the clade is less certain with two different positions identified in past studies. One recovered root has been placed between the nudiform family Acanthoecidae and the tectiform family Stephanoecidae. An alternative root placement falls within the tectiform species, recovering the monophyletic Acanthoecidae nested within a paraphyletic Stephanoecidae. Presented here is a 14-gene phylogeny, based upon nucleotide and amino acid sequences, which strongly supports tectiform paraphyly. The horizontal transfer of a ribosomal protein gene, from a possible SAR donor, into a subset of acanthoecid species provides further, independent, support for this root placement. Differing patterns of codon usage bias across the choanoflagellates are proposed as the cause of artefactual phylogenetic signals that lead to the recovery of tectiform monophyly.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6","pages":"Article 125924"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461022000694/pdfft?md5=d608422d26f85ac6b4fe59dad392ce1a&pid=1-s2.0-S1434461022000694-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10448075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Angled Twin–layer Porous Substrate Photobioreactors for Cultivation of Nannochloropsis oculata 培养纳米绿藻的新型倾斜双层多孔基质光生物反应器

IF 2.5 3区生物学

Protist Pub Date : 2022-12-01 DOI: 10.1016/j.protis.2022.125914

Hoang-Dung Tran , Binh-Nguyen Ong , Vinh-Tuong Ngo , Dai-Long Tran , Thanh-Cong Nguyen , Bich-Huy Tran-Thi , Thanh-Tri Do , Tran-Minh-Ly Nguyen , Xuan-Hoang Nguyen , Michael Melkonian

{"title":"New Angled Twin–layer Porous Substrate Photobioreactors for Cultivation of Nannochloropsis oculata","authors":"Hoang-Dung Tran , Binh-Nguyen Ong , Vinh-Tuong Ngo , Dai-Long Tran , Thanh-Cong Nguyen , Bich-Huy Tran-Thi , Thanh-Tri Do , Tran-Minh-Ly Nguyen , Xuan-Hoang Nguyen , Michael Melkonian","doi":"10.1016/j.protis.2022.125914","DOIUrl":"10.1016/j.protis.2022.125914","url":null,"abstract":"<div>An angled twin–layer porous substrate photobioreactor (TL-PSBR) using LED light was designed to cultivate Nannochloropsis oculata. Flocculation and sedimentation by modification of pH to 11 were determined as the optimal method for harvesting the N. oculata inoculum. The following optimised parameters were found: tilt angle 15°, Kraft 220 g m−2 paper as substrate material, initial inoculum density of 12.5 g m−2, 140 µmol photons m−2 s−1 light intensity, and a light/dark cycle of 6:6 (h). Test cultivation for 14 days was performed under optimised conditions. The total dried biomass standing crop was 75.5 g m−2 growth area with an average productivity of 6.3 g m−2 d−1, the productivity per volume of used culture medium was 126.2 mg/L d−1, total lipid content 21.9% (w/w), and the highest productivity of total lipids was 1.33 g m−2 d−1. The dry algal biomass contained 3% eicosapentaenoic acid (w/w), 3.7% palmitoleic acid (w/w), and 513 mg kg−1 vitamin E. The optimisation of N. oculata cultivation on an angled TL-PSBR system yielded promising results, and applications for commercial products need to be further explored.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6","pages":"Article 125914"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461022000591/pdfft?md5=68d97d66cbeeaa719a755c454acafa61&pid=1-s2.0-S1434461022000591-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10446321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Effect of pH and Salinity on the Toxicity and Growth of the Golden Alga, Prymnesium parvum. pH和盐度对金藻Prymnesium parvum毒性和生长的影响。

IF 2.5 3区生物学

Protist Pub Date : 2022-11-01 DOI: 10.2139/ssrn.4166586

D. Caron, Alle A. Y. Lie, T. Buckowski, J. Turner, Kevin Frabotta

引用次数: 2

Implications of a Catastrophic Refrigeration Failure on the Viability of Cryogenically Stored Samples. 灾难性制冷故障对低温储存样品生存能力的影响。

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.2139/ssrn.4142146

John G. Day, Katharine H. Childs, G. Stacey

{"title":"Implications of a Catastrophic Refrigeration Failure on the Viability of Cryogenically Stored Samples.","authors":"John G. Day, Katharine H. Childs, G. Stacey","doi":"10.2139/ssrn.4142146","DOIUrl":"https://doi.org/10.2139/ssrn.4142146","url":null,"abstract":"Cryopreservation, the use of very low temperatures to preserve structurally intact living cells and tissues, is a key underpinning technology for life science research and medicine. It is employed to ensure the stability of critical biological resources including viruses, bacteria, protists, animal cell cultures, plants, reproductive materials and embryos. Fundamental to ensuring this stability is assuring stability of cryogenic storage temperatures. Here we report the occurrence of a failure in refrigeration in a cryostat holding > 600 strains of cyanobacteria and eukaryotic microalgae. A strategic approach was adopted to assess viability across a cross-section of the biodiversity held, both immediately after the potentially damaging temperature shift and 10 years later, on subsequent cryostorage in liquid-phase nitrogen (∼-196 °C). Furthermore, the event was replicated experimentally and the effects on the viability of cryo-tolerant and cryo-sensitive strains monitored. Our results have significant implications to all users of this storage method and parallels have been drawn with the ongoing development in other fields and in particular, human cell therapy. Based on our practical experience we have made a series of generic recommendations for emergency, remedial and ongoing preventative actions.","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6 1","pages":"125915"},"PeriodicalIF":2.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48372898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved Methods for Bulk Cultivation and Fixation of Loxodes Ciliates for Fluorescence Microscopy 荧光显微镜下纤毛虫批量培养和固定的改进方法

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.1016/j.protis.2022.125905

Brandon Kwee Boon Seah, Christiane Emmerich, Aditi Singh, Estienne Carl Swart

引用次数: 0

High Environmentally Induced Plasticity in Spore Size and Numbers of Nuclei per Spore in Physarum albescens (Myxomycetes) 白色绒泡菌孢子大小和每孢子核数的高环境诱导可塑性

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.1016/j.protis.2022.125904

Jan Woyzichovski , Oleg N. Shchepin , Martin Schnittler

{"title":"High Environmentally Induced Plasticity in Spore Size and Numbers of Nuclei per Spore in Physarum albescens (Myxomycetes)","authors":"Jan Woyzichovski , Oleg N. Shchepin , Martin Schnittler","doi":"10.1016/j.protis.2022.125904","DOIUrl":"10.1016/j.protis.2022.125904","url":null,"abstract":"<div>Spore size enables dispersal in plasmodial slime molds (Myxomycetes) and is an important taxonomic character. We recorded size and the number of nuclei per spore for 39 specimens (colonies of 50–1000 sporocarps) of the nivicolous myxomycete Physarum albescens, a morphologically defined taxon with several biological species. For each colony, three sporocarps were analyzed from the same spore mount under brightfield and DAPI-fluorescence, recording ca. 14,000 spores per item. Diagrams for spore size distribution showed narrow peaks of mostly uninucleate spores. Size was highly variable within morphospecies (10.6–13.5 µm, 11–13%), biospecies (3–13%), even within spatially separated colonies of one clone (ca. 8%); but fairly constant for a colony (mean variation 0.4 µm, ca. 1.5%). ANOVA explains most of this variation by the factor locality (within all colonies: 32.7%; within a region: 21.4%), less by biospecies (13.5%), whereas the contribution of intra-colony variation was negligible (<0.1%). Two rare aberrations occur: 1) multinucleate spores and 2) oversized spores with a double or triple volume of normal spores. Both are not related to each other or limited to certain biospecies. Spore size shows high phenotypic plasticity, but the low variation within a colony points to a strong genetic background.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 5","pages":"Article 125904"},"PeriodicalIF":2.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10793544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bland James Finlay (March 16, 1952–December 24, 2021) 布兰德·詹姆斯·芬利(1952年3月16日- 2021年12月24日)

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.1016/j.protis.2022.125906

Tom Fenchel

引用次数: 0

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