IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.2139/ssrn.4142146

John G. Day, Katharine H. Childs, G. Stacey

{"title":"Implications of a Catastrophic Refrigeration Failure on the Viability of Cryogenically Stored Samples.","authors":"John G. Day, Katharine H. Childs, G. Stacey","doi":"10.2139/ssrn.4142146","DOIUrl":"https://doi.org/10.2139/ssrn.4142146","url":null,"abstract":"Cryopreservation, the use of very low temperatures to preserve structurally intact living cells and tissues, is a key underpinning technology for life science research and medicine. It is employed to ensure the stability of critical biological resources including viruses, bacteria, protists, animal cell cultures, plants, reproductive materials and embryos. Fundamental to ensuring this stability is assuring stability of cryogenic storage temperatures. Here we report the occurrence of a failure in refrigeration in a cryostat holding > 600 strains of cyanobacteria and eukaryotic microalgae. A strategic approach was adopted to assess viability across a cross-section of the biodiversity held, both immediately after the potentially damaging temperature shift and 10 years later, on subsequent cryostorage in liquid-phase nitrogen (∼-196 °C). Furthermore, the event was replicated experimentally and the effects on the viability of cryo-tolerant and cryo-sensitive strains monitored. Our results have significant implications to all users of this storage method and parallels have been drawn with the ongoing development in other fields and in particular, human cell therapy. Based on our practical experience we have made a series of generic recommendations for emergency, remedial and ongoing preventative actions.","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48372898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved Methods for Bulk Cultivation and Fixation of Loxodes Ciliates for Fluorescence Microscopy 荧光显微镜下纤毛虫批量培养和固定的改进方法

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.1016/j.protis.2022.125905

Brandon Kwee Boon Seah, Christiane Emmerich, Aditi Singh, Estienne Carl Swart

引用次数: 0

High Environmentally Induced Plasticity in Spore Size and Numbers of Nuclei per Spore in Physarum albescens (Myxomycetes) 白色绒泡菌孢子大小和每孢子核数的高环境诱导可塑性

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.1016/j.protis.2022.125904

Jan Woyzichovski , Oleg N. Shchepin , Martin Schnittler

{"title":"High Environmentally Induced Plasticity in Spore Size and Numbers of Nuclei per Spore in Physarum albescens (Myxomycetes)","authors":"Jan Woyzichovski , Oleg N. Shchepin , Martin Schnittler","doi":"10.1016/j.protis.2022.125904","DOIUrl":"10.1016/j.protis.2022.125904","url":null,"abstract":"<div>Spore size enables dispersal in plasmodial slime molds (Myxomycetes) and is an important taxonomic character. We recorded size and the number of nuclei per spore for 39 specimens (colonies of 50–1000 sporocarps) of the nivicolous myxomycete Physarum albescens, a morphologically defined taxon with several biological species. For each colony, three sporocarps were analyzed from the same spore mount under brightfield and DAPI-fluorescence, recording ca. 14,000 spores per item. Diagrams for spore size distribution showed narrow peaks of mostly uninucleate spores. Size was highly variable within morphospecies (10.6–13.5 µm, 11–13%), biospecies (3–13%), even within spatially separated colonies of one clone (ca. 8%); but fairly constant for a colony (mean variation 0.4 µm, ca. 1.5%). ANOVA explains most of this variation by the factor locality (within all colonies: 32.7%; within a region: 21.4%), less by biospecies (13.5%), whereas the contribution of intra-colony variation was negligible (<0.1%). Two rare aberrations occur: 1) multinucleate spores and 2) oversized spores with a double or triple volume of normal spores. Both are not related to each other or limited to certain biospecies. Spore size shows high phenotypic plasticity, but the low variation within a colony points to a strong genetic background.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10793544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bland James Finlay (March 16, 1952–December 24, 2021) 布兰德·詹姆斯·芬利(1952年3月16日- 2021年12月24日)

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.1016/j.protis.2022.125906

Tom Fenchel

引用次数: 0

Significance of the Nudiform and Tectiform Modes of Silica Deposition, Lorica Assembly and Cell Division in Choanoflagellates as Exemplified by Stephanoeca diplocostata and Enibas spp. 以Stephanoeca diplocostata和enspp为例的毛鞭毛虫裸形和隐形二氧化硅沉积、Lorica组装和细胞分裂模式的意义

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.2139/ssrn.4152857

B. Leadbeater, M. Carr

{"title":"Significance of the Nudiform and Tectiform Modes of Silica Deposition, Lorica Assembly and Cell Division in Choanoflagellates as Exemplified by Stephanoeca diplocostata and Enibas spp.","authors":"B. Leadbeater, M. Carr","doi":"10.2139/ssrn.4152857","DOIUrl":"https://doi.org/10.2139/ssrn.4152857","url":null,"abstract":"The deposition of silicified costal strips and lorica assembly in choanoflagellates is precisely linked to the cell cycle. A minority of species undergo nudiform division whereby a loricate cell divides to produce a naked daughter cell that deposits a set of costal strips and then assembles a lorica. Most species undergo tectiform division whereby a parent loricate cell produces a set of costal strips, divides and passes on the stored strips to a daughter cell that immediately assembles a lorica. Many phylogenetic analyses recover nudiform and tectiform species as sister-clades giving the impression that they are distinct evolutionary lineages. However, the tectiform species Stephanoeca diplocostata is capable of undergoing nudiform division and depositing costal strips and assembling a lorica with certain modifications in a nudiform manner. The recent discovery of a new genus, Enibas, comprising species with Stephanoeca-like loricae that undergo nudiform cell division and on phylogenetic analysis occur as a sister group to other nudiform species has drawn attention to whether there are also unique features in lorica construction. A range of Enibas loricae is illustrated and it appears that there are unique features which might be interpreted as being derived from a Stephanoeca-like ancestor.","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47877328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Unravelling the Phylogeny of a Common Intestinal Protist: Intrageneric Diversity of Endolimax 揭示一种常见肠道原生生物的系统发育:Endolimax的属内多样性

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.1016/j.protis.2022.125908

Emma Filtenborg Hocke , Mahwash Jamy , Fabien Burki , C. Graham Clark , Christen Rune Stensvold

{"title":"Unravelling the Phylogeny of a Common Intestinal Protist: Intrageneric Diversity of Endolimax","authors":"Emma Filtenborg Hocke , Mahwash Jamy , Fabien Burki , C. Graham Clark , Christen Rune Stensvold","doi":"10.1016/j.protis.2022.125908","DOIUrl":"10.1016/j.protis.2022.125908","url":null,"abstract":"<div>Endolimax nana is a common endobiont of the human intestine, but members of the genus have also been reported in non-human hosts and in non-intestinal organs. Limited information is available regarding the genetic diversity of Endolimax, which is necessary to delineate species, host specificity and potential differences in clinical impact on the host. Here, we used cloning of PCR products followed by Sanger sequencing and next-generation PacBio Sequencing to obtain Endolimax-related nuclear ribosomal gene sequences and undertook a phylogenetic analysis to gain additional insight into the taxonomy of Endolimax and related organisms. The new sequences confirmed that E. nana forms a discrete clade within the Archamoebae and is related to Endolimax piscium and Iodamoeba. However, we identified substantial sequence divergence within E. nana and evidence for two distinct clades, which we propose to name E. nana ribosomal lineage 1 and E. nana ribosomal lineage 2. Both of the sequencing approaches applied in the study helped us to improve our understanding of genetic diversity across Endolimax, and it is likely that wider application of next-generation sequencing technologies will facilitate the generation of Endolimax-related DNA sequence data and help complete our understanding of its phylogenetic position and intrageneric diversity.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10813211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Integrative Studies on Three Epibiotic Epistylis Species (Protozoa, Ciliophora, Peritrichia) in Lake Weishan Wetland, Northern China, Including the Establishment of a New Species 微山湖湿地3种附着生物Epistylis (Protozoa, Ciliophora, Peritrichia)的综合研究及一个新种的建立

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.1016/j.protis.2022.125909

Tong Wu , Yujie Liu , Yan Liu , Borong Lu , Xiao Cao , Shuang Hao , Saleh A. AL-Farraj , Alan Warren , Tengteng Zhang , Zhe Wang

{"title":"Integrative Studies on Three Epibiotic Epistylis Species (Protozoa, Ciliophora, Peritrichia) in Lake Weishan Wetland, Northern China, Including the Establishment of a New Species","authors":"Tong Wu , Yujie Liu , Yan Liu , Borong Lu , Xiao Cao , Shuang Hao , Saleh A. AL-Farraj , Alan Warren , Tengteng Zhang , Zhe Wang","doi":"10.1016/j.protis.2022.125909","DOIUrl":"10.1016/j.protis.2022.125909","url":null,"abstract":"<div>Three epibiotic Epistylis species, i.e., Epistylis weishanensis sp. nov., Epistylis daphniae Fauré-Fremiet, 1905, and Epistylis pygmaeum (Ehrenberg, 1838) Foissner et al., 1999, were investigated based on their living morphology, infraciliature, and small subunit (SSU) rDNA sequence data. Epistylis weishanensis sp. nov. is characterized by its double-layered peristomial lip, contractile vacuole located on the dorsal wall of the infundibulum, infundibular polykinety 3 (P3) composed of three equal-length rows that terminate above infundibular polykinety 1 (P1), 50–62 silverlines between the peristome and the trochal band, and about 30 silverlines between the trochal band and the scopula. Based on previous and newly obtained data for E. daphniae and E. pygmaeum, improved diagnoses and redescriptions are provided including, for the first time, data on their infraciliature. Phylogenetic analyses reveal that all three species do not group within the major clade of Epistylis, supporting the assertion that the genus Epistylis should be an assemblage of morphospecies and therefore needs to be revised.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10813654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Illustrating the Invisible: The Role of Drawing in the Career of Bland J. Finlay 阐释无形:绘画在布兰德·j·芬利职业生涯中的作用

IF 2.5 3区生物学

Protist Pub Date : 2022-10-01 DOI: 10.1016/j.protis.2022.125907

Carlos F. Finlay

引用次数: 0

Some Personal Memories of Bland J. Finlay and Work on Protists in "Priest Pot". 布兰德·J·芬莱的一些个人回忆和《牧师壶》中的主人公作品。

IF 2.5 3区生物学

Protist Pub Date : 2022-09-01 DOI: 10.2139/ssrn.4208046

U. Berninger

引用次数: 0

The Extraordinarily Rare Ciliate Legendrea loyezae Fauré-Fremiet, 1908 (Haptoria, Ciliophora). 特别罕见的Ciliate Legendarea loyezae Fauré-Fremiet，1908年（Haptoria，Ciliophora）。

IF 2.5 3区生物学

Protist Pub Date : 2022-09-01 DOI: 10.2139/ssrn.4163417

James Weiss, D. Andreou, G. Esteban

{"title":"The Extraordinarily Rare Ciliate Legendrea loyezae Fauré-Fremiet, 1908 (Haptoria, Ciliophora).","authors":"James Weiss, D. Andreou, G. Esteban","doi":"10.2139/ssrn.4163417","DOIUrl":"https://doi.org/10.2139/ssrn.4163417","url":null,"abstract":"Diverse and dynamic communities of ciliates and other microbes thrive in the natural environment, driving the functioning of aquatic ecosystems. Many microbes are present in very low numbers or are dormant in the 'seedbank', escaping detection in environmental surveys and, consequently, remaining underexplored. Here, we report an extraordinarily rare ciliate that was discovered after persistent exploration of freshwater anoxic sediments - Legendrea loyezae Fauré-Fremiet, 1908, a member of the Family Spathidiidae, Order Haptorida. In this study, we present the sixth account of the ciliate since 1908 and reveal its phylogenetic position with the first 18S rRNA data for the genus. We explain the key morphological features of the species, describing a remarkable behaviour in which the ciliate \"shapeshifts'' due to its ability of controlled full extension and retraction of its tube-like tentacles. Our results shed light on the similarity of L. loyezae to another ciliate that was first described as Legendrea bellerophon, later moved under a new genus and named Thysanomorpha bellerophon. We question the validity of this taxonomic decision and, based on morphological characters and tentacle movement, we propose moving T. bellerophon back under Legendrea. This study demonstrates how continued and persistent exploration of natural habitats lead to the discovery of microbial communities and species.","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41255970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

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