ProtistPub Date : 2023-04-01DOI: 10.1016/j.protis.2023.125947
R. Ellen R. Nisbet , Christopher J. Howe
{"title":"25 years of Protist: A thank you to Editor-in-Chief Michael Melkonian","authors":"R. Ellen R. Nisbet , Christopher J. Howe","doi":"10.1016/j.protis.2023.125947","DOIUrl":"10.1016/j.protis.2023.125947","url":null,"abstract":"","PeriodicalId":20781,"journal":{"name":"Protist","volume":"174 2","pages":"Article 125947"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9418164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ProtistPub Date : 2023-04-01DOI: 10.1016/j.protis.2023.125948
Fan Yang , Jing-min Gou , Bing-ke Yang , Jia-yue Du , Hui-zhong Yao , Mei Ren , Qing Lin
{"title":"Prevalence and Subtype Distribution of Blastocystis in Tibetan Sheep in Qinghai Province, Northwestern China","authors":"Fan Yang , Jing-min Gou , Bing-ke Yang , Jia-yue Du , Hui-zhong Yao , Mei Ren , Qing Lin","doi":"10.1016/j.protis.2023.125948","DOIUrl":"10.1016/j.protis.2023.125948","url":null,"abstract":"<div><p><em>Blastocystis</em><span> is one of the most common intestinal protists in humans and a great number of animals, including sheep and goats. High prevalence and multiple subtypes of </span><em>Blastocystis</em> have been reported in sheep in several regions of China and elsewhere. However, there is a dearth of knowledge about <em>Blastocystis</em> in Tibetan sheep. A total of 761 fecal samples were collected from Tibetan sheep in seven counties of Qinghai Province, northwestern China, and were examined for the prevalence and subtypes of <em>Blastocystis</em><span> using molecular technology based on the partial small subunit ribosomal RNA gene of </span><em>Blastocystis</em>. The overall prevalence of <em>Blastocystis</em><span> in the investigated Tibetan sheep was 7.5% (57/761) using PCR and DNA Sanger sequencing, and differences in prevalence were observed among the ruminants from the seven counties (</span><em>P</em> < 0.01), and across four seasons (<em>P</em><span> < 0.01). Sequence analysis revealed five subtypes (ST14 (57.9%), ST10 (26.3%), ST12 (5.3%), ST21 (5.3%), and ST30 (5.3%)) of </span><em>Blastocystis</em> sp. in these Tibetan sheep, with ST14 as the predominant subtype. To our knowledge, this is the first report of <em>Blastocystis</em> colonization in Tibetan sheep.</p></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"174 2","pages":"Article 125948"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9627175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ProtistPub Date : 2023-02-01DOI: 10.1016/j.protis.2022.125927
David A. Caron , Alle A.Y. Lie , Tom Buckowski , Jim Turner , Kevin Frabotta
{"title":"The Effect of pH and Salinity on the Toxicity and Growth of the Golden Alga, Prymnesium parvum","authors":"David A. Caron , Alle A.Y. Lie , Tom Buckowski , Jim Turner , Kevin Frabotta","doi":"10.1016/j.protis.2022.125927","DOIUrl":"10.1016/j.protis.2022.125927","url":null,"abstract":"<div><p>Bioassays using cultures of the toxic haptophyte <em>Prymnesium parvum</em> and the ciliate <em>Cyclidium</em> sp. as prey were conducted to test the effect of pH (range = 6.5 – 8.5), salinity (range = 1.50 – 7.50‰), and a combination of pH and salinity on the toxicity of <em>P. parvum</em>. pH had a significant effect on <em>P. parvum</em> toxicity. Toxicity was rapidly (within 24 hr) induced by increasing pH of the medium, or reduced by lowering pH. Conversely, lowering salinity reduced toxicity, albeit less effectively compared to pH, and <em>P. parvum</em> cells remained toxic at the lowest values tested (1.50‰ at pH 7.5). An additional effect between pH and salinity was also observed: low salinity combined with low pH led to not only decreased toxicity, but also resulted in lower <em>P. parvum</em> growth rates. Such effects of pH and salinity on <em>P. parvum</em> growth and toxicity provide insight into the environmental factors supporting community dominance and toxic blooms of the alga.</p></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"174 1","pages":"Article 125927"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9353294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ProtistPub Date : 2023-02-01DOI: 10.1016/j.protis.2022.125926
Genoveva F. Esteban, Stephen C. Maberly
{"title":"Introduction to the Special Issue: Bland J. Finlay: Uncovering the Unseen World of Microbes","authors":"Genoveva F. Esteban, Stephen C. Maberly","doi":"10.1016/j.protis.2022.125926","DOIUrl":"10.1016/j.protis.2022.125926","url":null,"abstract":"","PeriodicalId":20781,"journal":{"name":"Protist","volume":"174 1","pages":"Article 125926"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9252839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ProtistPub Date : 2023-02-01DOI: 10.1016/j.protis.2022.125929
Blanca Pérez-Uz , Virginia C. Galfione , Raul Ochoa-Hueso , Mercedes Martín-Cereceda
{"title":"Protist Diversity Responses to Experimental N Deposition in Biological Crusts of a Semiarid Mediterranean Ecosystem","authors":"Blanca Pérez-Uz , Virginia C. Galfione , Raul Ochoa-Hueso , Mercedes Martín-Cereceda","doi":"10.1016/j.protis.2022.125929","DOIUrl":"10.1016/j.protis.2022.125929","url":null,"abstract":"<div><p>Biological soil crusts (BSC) are associations of different macro and microorganisms and aggregated soil particles located on the surface of soils in many different habitats. BSC harbour a diverse and complex community of ciliates and testate amoebae. These phagotrophic protists play an important role in C and N recycling in soil ecosystems but have not been frequently studied in BSC. In this context, the effects of three increasing N inputs on ciliates and testate amoebae in crusts from a semi-arid Mediterranean ecosystem were evaluated. A field experiment with artificial N-deposition was designed to mimic the effects caused by anthropogenic N depositions. The results have shown that the protist populations of these semi-arid Mediterranean environments have lower species richness than other soil environments. The increase in N produces a net loss of diversity in the populations studied and shifts in the community structure. It has also been shown that some ciliates and testate amoebae, due to their population responses to increased N concentrations, could potentially be used as bio-indicators of N contamination in these BSCs.</p></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"174 1","pages":"Article 125929"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9411431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ProtistPub Date : 2023-02-01DOI: 10.1016/j.protis.2022.125928
Maira Maselli , Lorenz Meire , Patrick Meire , Per Juel Hansen
{"title":"Effects of Glacial Flour on Marine Micro-plankton: Evidences from Natural Communities of Greenlandic Fjords and Experimental Studies","authors":"Maira Maselli , Lorenz Meire , Patrick Meire , Per Juel Hansen","doi":"10.1016/j.protis.2022.125928","DOIUrl":"10.1016/j.protis.2022.125928","url":null,"abstract":"<div><p>Meltwater runoff from glaciers carries particles, so-called glacial flour that may affect planktonic organisms and the functioning of marine ecosystems. Protist microplankton is at the base of marine food webs and thus plays an important role in sustaining important ecosystem services. To assess the effect of glacial flour on photoautotrophic, heterotrophic and mixotrophic microplankton, the spatial distribution of these trophic groups was studied in four Greenlandic fjords during summer. The results suggest that the abundance of the autotrophic microplankton was affected by the glacier meltwater due to reduced light penetration and nutrient availability. The abundance of heterotrophic and mixotrophic microplankton were not apparently affected by the glacier meltwater. Incubation experiments were conducted on the natural population and in laboratory cultures of two mixoplanktonic ciliate species. The experiments on the natural population revealed that none of the trophic groups were affected by the suspended material at concentrations up to 50 mg L<sup>−1</sup>. The experiments on cultures gave no indication that glacial flour was ingested by the mixoplanktonic ciliates. Growth rates of cultured ciliates were not affected by the glacial flour addition. These results suggest that heterotrophic and mixotrophic microplankton are not affected by glacial flour as much as autotrophic microplankton.</p></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"174 1","pages":"Article 125928"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9723706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ProtistPub Date : 2022-12-01DOI: 10.1016/j.protis.2022.125912
James Weiss , Demetra Andreou , Genoveva F. Esteban
{"title":"The Extraordinarily Rare Ciliate Legendrea loyezae Fauré-Fremiet, 1908 (Haptoria, Ciliophora)","authors":"James Weiss , Demetra Andreou , Genoveva F. Esteban","doi":"10.1016/j.protis.2022.125912","DOIUrl":"10.1016/j.protis.2022.125912","url":null,"abstract":"<div><p>Diverse and dynamic communities of ciliates and other microbes thrive in the natural environment, driving the functioning of aquatic ecosystems. Many microbes are present in very low numbers or are dormant in the ‘seedbank’, escaping detection in environmental surveys and, consequently, remaining underexplored. Here, we report an extraordinarily rare ciliate that was discovered after persistent exploration of freshwater anoxic sediments - <em>Legendrea loyezae</em> Fauré-Fremiet, 1908, a member of the Family Spathidiidae, Order Haptorida. In this study, we present the sixth account of the ciliate since 1908 and reveal its phylogenetic position with the first 18S rRNA data for the genus. We explain the key morphological features of the species, describing a remarkable behaviour in which the ciliate “shapeshifts'' due to its ability of controlled full extension and retraction of its tube-like tentacles. Our results shed light on the similarity of <em>L. loyezae</em> to another ciliate that was first described as <em>Legendrea bellerophon</em>, later moved under a new genus and named <em>Thysanomorpha bellerophon</em>. We question the validity of this taxonomic decision and, based on morphological characters and tentacle movement, we propose moving <em>T. bellerophon</em> back under <em>Legendrea</em>. This study demonstrates how continued and persistent exploration of natural habitats lead to the discovery of microbial communities and species.</p></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6","pages":"Article 125912"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461022000578/pdfft?md5=1087f5f4243f46e37d2598674145c135&pid=1-s2.0-S1434461022000578-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10448044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ProtistPub Date : 2022-12-01DOI: 10.1016/j.protis.2022.125925
Stephen C. Maberly , Anne Chao , Bland J. Finlay
{"title":"Seasonal Patterns of Phytoplankton Taxon Richness in Lakes: Effects of Temperature, Turnover and Abundance","authors":"Stephen C. Maberly , Anne Chao , Bland J. Finlay","doi":"10.1016/j.protis.2022.125925","DOIUrl":"10.1016/j.protis.2022.125925","url":null,"abstract":"<div><p>Species richness is a key ecological characteristic that influences numerous ecosystem functions. Here we analyse the patterns and possible causes of phytoplankton taxon richness in seasonal datasets from twenty contrasting lakes in the English Lake District over six years and near-weekly datasets over 33 years from Windermere. Taxon richness was lowest in winter and highest in summer or autumn in all of the lakes. Observed richness was very similar to richness estimated from coverage and sampling effort, implying that it closely reflected true seasonal patterns. Summer populations were dominated by Chlorophyta and functional groups X1, F, N and P (<em>sensu</em> Reynolds). In Windermere, weekly taxon richness was strongly positively correlated with surface water temperature, as was the number of functional groups and the number of taxa per functional group. Turnover in richness of taxa and functional groups were positively correlated and both were related to surface temperature. This suggests that high taxon richness in summer is linked to higher water temperature, promoting a turnover in richness of taxa and functional groups in these lakes. However, since the number of taxa per unit concentration of chlorophyll <em>a</em> decreased with increasing concentration of chlorophyll <em>a</em>, competition might occur when abundance is high.</p></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6","pages":"Article 125925"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461022000700/pdfft?md5=5a903e2a4160eda54e94ea932c611e85&pid=1-s2.0-S1434461022000700-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10504941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ProtistPub Date : 2022-12-01DOI: 10.1016/j.protis.2022.125910
Ulrike-G. Berninger
{"title":"Some Personal Memories of Bland J. Finlay and Work on Protists in “Priest Pot“","authors":"Ulrike-G. Berninger","doi":"10.1016/j.protis.2022.125910","DOIUrl":"https://doi.org/10.1016/j.protis.2022.125910","url":null,"abstract":"<div><p>With his scientific curiosity and deep understanding of unicellular organisms, Bland J. Finlay could demonstrate that water bodies as small and innocuous as “Priest Pot“ in the English Lake District represent a very broad range of protists and their interactions. From this, not only the field of protistan ecology benefitted greatly, but also my personal career. Enclosed are some scientific and personal memories to say ”thank you” to Bland.</p></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6","pages":"Article 125910"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461022000554/pdfft?md5=17b5859381a6b5574fdaae4864fc2c59&pid=1-s2.0-S1434461022000554-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72203586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ProtistPub Date : 2022-12-01DOI: 10.1016/j.protis.2022.125911
Anatoly Bobrov , Sebastian Wetterich , Lutz Schirrmeister
{"title":"Testate Amoebae in Historical parks of Potsdam, Germany","authors":"Anatoly Bobrov , Sebastian Wetterich , Lutz Schirrmeister","doi":"10.1016/j.protis.2022.125911","DOIUrl":"10.1016/j.protis.2022.125911","url":null,"abstract":"<div><p>To explore the potential of urban settings as habitats for testate amoebae, five historical parks in Potsdam (Germany) were sampled at different sites. A total of 32 sampling sites was chosen in proximity to deciduous (<em>Acer</em>, <em>Castanea</em>, <em>Fagus</em>, <em>Tilia</em>, <em>Platanus</em>, <em>Quercus</em>) and coniferous (<em>Fraxinus</em>, <em>Picea</em>, <em>Pinus</em>, <em>Tsuga</em>) trees. Meadows and creeks were also sampled. The overall taxonomic record comprises 76 species and sub-species. High species numbers of >20 per sample were found in meadows and below <em>Fagus</em>, <em>Tilia,</em> and <em>Quercus</em> trees. The species richness per park ranges from 33 to 46 taxa. Most species belong to the eurybiontic ecological group, although litter-inhabiting and hygrophilic and hydrophilic species were also present. Common species found in more than 50% of all samples (superdominants) belong to the genera <em>Centropyxis</em>, <em>Cyclopyxis</em>, <em>Euglypha,</em> and <em>Trinema</em>. Interestingly, the rare <em>Frenopyxis stierlitzi</em> which inhabits tree hollows was found as a recently described species in a new genus <em>Frenopyxis</em> <span>Bobrov</span> <span>&</span> <span>Mazei</span> <span>2020</span> in the Babelsberg Park. The studied testate amoebae are characterized by a high degree of morphological and morphometric plasticity. Therefore, the study of testate amoebae in urban settings will reveal new insights into their ecology and enhance the definition of morphometric variability for single species.</p></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"173 6","pages":"Article 125911"},"PeriodicalIF":2.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461022000566/pdfft?md5=17f72b01d0bf8c49f85812ea2966de96&pid=1-s2.0-S1434461022000566-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10447520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}