Protein Science最新文献_第5页

Exploring protein functions from structural flexibility using CABS-flex modeling. 利用 CABS-flex 建模从结构灵活性中探索蛋白质功能。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-09-01 DOI: 10.1002/pro.5090

Chandran Nithin, Rocco Peter Fornari, Smita P Pilla, Karol Wroblewski, Mateusz Zalewski, Rafał Madaj, Andrzej Kolinski, Joanna M Macnar, Sebastian Kmiecik

{"title":"Exploring protein functions from structural flexibility using CABS-flex modeling.","authors":"Chandran Nithin, Rocco Peter Fornari, Smita P Pilla, Karol Wroblewski, Mateusz Zalewski, Rafał Madaj, Andrzej Kolinski, Joanna M Macnar, Sebastian Kmiecik","doi":"10.1002/pro.5090","DOIUrl":"10.1002/pro.5090","url":null,"abstract":"Understanding protein function often necessitates characterizing the flexibility of protein structures. However, simulating protein flexibility poses significant challenges due to the complex dynamics of protein systems, requiring extensive computational resources and accurate modeling techniques. In response to these challenges, the CABS-flex method has been developed as an efficient modeling tool that combines coarse-grained simulations with all-atom detail. Available both as a web server and a standalone package, CABS-flex is dedicated to a wide range of users. The web server version offers an accessible interface for straightforward tasks, while the standalone command-line program is designed for advanced users, providing additional features, analytical tools, and support for handling large systems. This paper examines the application of CABS-flex across various structure-function studies, facilitating investigations into the interplay among protein structure, dynamics, and function in diverse research fields. We present an overview of the current status of the CABS-flex methodology, highlighting its recent advancements, practical applications, and forthcoming challenges.","PeriodicalId":20761,"journal":{"name":"Protein Science","volume":"33 9","pages":"e5090"},"PeriodicalIF":4.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11350595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zika virus capsid protein closed structure modulates binding to host lipid systems. 寨卡病毒帽状蛋白封闭结构调节与宿主脂质系统的结合。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-09-01 DOI: 10.1002/pro.5142

Ana S Martins, Filomena A Carvalho, André R Nascimento, Nelly M Silva, Teresa V Rebelo, André F Faustino, Francisco J Enguita, Roland G Huber, Nuno C Santos, Ivo C Martins

{"title":"Zika virus capsid protein closed structure modulates binding to host lipid systems.","authors":"Ana S Martins, Filomena A Carvalho, André R Nascimento, Nelly M Silva, Teresa V Rebelo, André F Faustino, Francisco J Enguita, Roland G Huber, Nuno C Santos, Ivo C Martins","doi":"10.1002/pro.5142","DOIUrl":"10.1002/pro.5142","url":null,"abstract":"Zika virus (ZIKV), a mosquito-borne Flavivirus of international concern, causes congenital microcephaly in newborns and Guillain-Barré syndrome in adults. ZIKV capsid (C) protein, one of three key structural proteins, is essential for viral assembly and encapsidation. In dengue virus, a closely related flavivirus, the homologous C protein interacts with host lipid systems, namely intracellular lipid droplets, for successful viral replication. Here, we investigate ZIKV C interaction with host lipid systems, showing that it binds host lipid droplets but, contrary to expected, in an unspecific manner. Contrasting with other flaviviruses, ZIKV C also does not bind very-low density-lipoproteins. Comparing with other Flavivirus, capsid proteins show that ZIKV C structure is particularly thermostable and seems to be locked into an auto-inhibitory conformation due to a disordered N-terminal, hence blocking specific interactions and supporting the experimental differences observed. Such distinct structural features must be considered when targeting capsid proteins in drug development.","PeriodicalId":20761,"journal":{"name":"Protein Science","volume":"33 9","pages":"e5142"},"PeriodicalIF":4.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11350591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Red/green cyanobacteriochromes acquire isomerization from phycocyanobilin to phycoviolobilin. 红/绿蓝藻色素从藻氰基生物素异构为藻氰基生物素。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-08-01 DOI: 10.1002/pro.5132

Hiroki Hoshino, Keita Miyake, Keiji Fushimi, Rei Narikawa

{"title":"Red/green cyanobacteriochromes acquire isomerization from phycocyanobilin to phycoviolobilin.","authors":"Hiroki Hoshino, Keita Miyake, Keiji Fushimi, Rei Narikawa","doi":"10.1002/pro.5132","DOIUrl":"10.1002/pro.5132","url":null,"abstract":"Cyanobacteriochromes (CBCRs) are unique cyanobacteria-specific photoreceptors that share a distant relation with phytochromes. Most CBCRs contain conserved cysteine residues known as canonical Cys, while some CBCRs have additional cysteine residues called second Cys within the DXCF motif, leading to their classification as DXCF CBCRs. They typically undergo a process where they incorporate phycocyanobilin (PCB) and subsequently isomerize it to phycoviolobilin (PVB). Conversely, CBCRs with conserved Trp residues and without the second Cys are called extended red/green (XRG) CBCRs. Typical XRG CBCRs bind PCB without undergoing PCB-to-PVB isomerization, displaying red/green reversible photoconversion, and there are also atypical CBCRs that exhibit diverse photoconversions. We discovered novel XRG CBCRs with Cys residue instead of the conserved Trp residue. These novel XRG CBCRs exhibited the ability to isomerize PCB to PVB, displaying green/teal reversible photoconversion. Through sequence- and structure-based comparisons coupled with mutagenesis experiments, we identified three amino acid residues, including the Cys residue, crucial for facilitating PCB-to-PVB isomerization. This research expands our understanding of the diversity of XRG CBCRs, highlighting the remarkable molecular plasticity of CBCRs.","PeriodicalId":20761,"journal":{"name":"Protein Science","volume":"33 8","pages":"e5132"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11284453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-based design of a Plasmodium vivax Duffy-binding protein immunogen focuses the antibody response to functional epitopes. 基于结构设计的间日疟原虫达菲结合蛋白免疫原可将抗体反应集中于功能表位。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-08-01 DOI: 10.1002/pro.5095

Thayne H Dickey, Holly McAleese, Nichole D Salinas, Lynn E Lambert, Niraj H Tolia

{"title":"Structure-based design of a Plasmodium vivax Duffy-binding protein immunogen focuses the antibody response to functional epitopes.","authors":"Thayne H Dickey, Holly McAleese, Nichole D Salinas, Lynn E Lambert, Niraj H Tolia","doi":"10.1002/pro.5095","DOIUrl":"10.1002/pro.5095","url":null,"abstract":"The Duffy-binding protein (DBP) is a promising antigen for a malaria vaccine that would protect against clinical symptoms caused by Plasmodium vivax infection. Region II of DBP (DBP-II) contains the receptor-binding domain that engages host red blood cells, but DBP-II vaccines elicit many non-neutralizing antibodies that bind distal to the receptor-binding surface. Here, we engineered a truncated DBP-II immunogen that focuses the immune response to the receptor-binding surface. This immunogen contains the receptor-binding subdomain S1S2 and lacks the immunodominant subdomain S3. Structure-based computational design of S1S2 identified combinatorial amino acid changes that stabilized the isolated S1S2 without perturbing neutralizing epitopes. This immunogen elicited DBP-II-specific antibodies in immunized mice that were significantly enriched for blocking activity compared to the native DBP-II antigen. This generalizable design process successfully stabilized an integral core fragment of a protein and focused the immune response to desired epitopes to create a promising new antigen for malaria vaccine development.","PeriodicalId":20761,"journal":{"name":"Protein Science","volume":"33 8","pages":"e5095"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11237555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stable and reusable calcium-responsive biopolymer for affinity precipitation of therapeutic antibodies. 用于治疗性抗体亲和沉淀的稳定且可重复使用的钙响应生物聚合物。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-08-01 DOI: 10.1002/pro.5066

Heesun Park, Jeong-Seok Oh, Jonghwan Lee, Jinho Bang, Keunwan Park, Suhyeon Jeong, Seho Park, Jae-Sung Woo, Sunghyun Kim

{"title":"Stable and reusable calcium-responsive biopolymer for affinity precipitation of therapeutic antibodies.","authors":"Heesun Park, Jeong-Seok Oh, Jonghwan Lee, Jinho Bang, Keunwan Park, Suhyeon Jeong, Seho Park, Jae-Sung Woo, Sunghyun Kim","doi":"10.1002/pro.5066","DOIUrl":"10.1002/pro.5066","url":null,"abstract":"Affinity precipitation is an attractive method for protein purification due to its many advantages, including the rapid capture of target proteins, simple processing, high specificity, and ease of scale-up. We previously reported a robust antibody purification method using Ca2+-dependent precipitation of ZZ-hCSQ2, a fusion protein of human calsequestrin 2, and the antibody-binding protein ZZ. However, the stability of this fusion protein was not sufficiently high for industrial use because the antibody recovery yield decreased to 60% after being reused 10 times. To identify a more stable calsequestrin (CSQ), we calculated Rosetta energy values for the folding stabilities of various CSQ homologs and selected human CSQ1 (hCSQ1) with lowest energy value (-992.6) as the new CSQ platform. We also identified that the linker sequence between ZZ and CSQ was vulnerable to proteases and alkaline pH by N-terminal protein sequencing. Therefore, we changed the linker to four asparagine (4N) sequences, which were shorter and less flexible than the previous glycine-rich linker. The new version of ZZ-CSQ, ZZ-4N-hCSQ1, was stable in a protease-containing conditioned medium obtained from the cultured Chinese hamster ovary cell or high pH condition (0.1M sodium hydroxide) for more than 5 days and could be reused at least 25 times for antibody purification without loss of recovery yield. The antibodies purified by ZZ-4N-hCSQ1 precipitation also showed greater purity (~33.6-fold lower host cell DNA and ~6.4-fold lower host cell protein) than those purified by protein A chromatography. These data suggest that ZZ-4N-hCSQ1 precipitation is more efficient and can achieve cost-effectiveness of up to 12.5-fold cheaper than previous antibody purification methods and can lower the production costs of therapeutic antibodies.","PeriodicalId":20761,"journal":{"name":"Protein Science","volume":"33 8","pages":"e5066"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11285868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EFG-CS: Predicting chemical shifts from amino acid sequences with protein structure prediction using machine learning and deep learning models. EFG-CS：利用机器学习和深度学习模型从氨基酸序列预测化学位移与蛋白质结构预测。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-08-01 DOI: 10.1002/pro.5096

Xiaotong Gu, Yoochan Myung, Carlos H M Rodrigues, David B Ascher

{"title":"EFG-CS: Predicting chemical shifts from amino acid sequences with protein structure prediction using machine learning and deep learning models.","authors":"Xiaotong Gu, Yoochan Myung, Carlos H M Rodrigues, David B Ascher","doi":"10.1002/pro.5096","DOIUrl":"10.1002/pro.5096","url":null,"abstract":"Nuclear magnetic resonance (NMR) crystallography is one of the main methods in structural biology for analyzing protein stereochemistry and structure. The chemical shift of the resonance frequency reflects the effect of the protons in a molecule producing distinct NMR signals in different chemical environments. Apprehending chemical shifts from NMR signals can be challenging since having an NMR structure does not necessarily provide all the required chemical shift information, making predictive models essential for accurately deducing chemical shifts, either from protein structures or, more ideally, directly from amino acid sequences. Here, we present EFG-CS, a web server that specializes in chemical shift prediction. EFG-CS employs a machine learning-based transfer prediction model for backbone atom chemical shift prediction, using ESMFold-predicted protein structures. Additionally, ESG-CS incorporates a graph neural network-based model to provide comprehensive side-chain atom chemical shift predictions. Our method demonstrated reliable performance in backbone atom prediction, achieving comparable accuracy levels with root mean square errors (RMSE) of 0.30 ppm for H, 0.22 ppm for Hα, 0.89 ppm for C, 0.89 ppm for Cα, 0.84 ppm for Cβ, and 1.69 ppm for N. Moreover, our approach also showed predictive capabilities in side-chain atom chemical shift prediction achieving RMSE values of 0.71 ppm for Hβ, 0.74-1.15 ppm for Hδ, and 0.58-0.94 ppm for Hγ, solely utilizing amino acid sequences without homology or feature curation. This work shows for the first time that generative AI protein models can predict NMR shifts nearly comparable to experimental models. This web server is freely available at https://biosig.lab.uq.edu.au/efg_cs, and the chemical shift prediction results can be downloaded in tabular format and visualized in 3D format.","PeriodicalId":20761,"journal":{"name":"Protein Science","volume":"33 8","pages":"e5096"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141559577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MRS2 missense variation at Asp216 abrogates inhibitory Mg²⁺ binding, potentiating cell migration and apoptosis resistance. MRS2在Asp216处的错义变异削弱了Mg2+的抑制性结合，从而增强了细胞迁移和抗凋亡能力。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-08-01 DOI: 10.1002/pro.5108

Sukanthathulse Uthayabalan, Taylor Lake, Peter B Stathopulos

{"title":"MRS2 missense variation at Asp216 abrogates inhibitory Mg2+ binding, potentiating cell migration and apoptosis resistance.","authors":"Sukanthathulse Uthayabalan, Taylor Lake, Peter B Stathopulos","doi":"10.1002/pro.5108","DOIUrl":"10.1002/pro.5108","url":null,"abstract":"Mitochondrial magnesium (Mg2+) is a crucial modulator of protein stability, enzymatic activity, ATP synthesis, and cell death. Mitochondrial RNA splicing protein 2 (MRS2) is the main Mg2+ channel in the inner mitochondrial membrane that mediates influx into the matrix. Recent cryo-electron microscopy (cryo-EM) human MRS2 structures exhibit minimal conformational changes at high and low Mg2+, yet the regulation of human MRS2 and orthologues by Mg2+ binding to analogous matrix domains has been well established. Further, a missense variation at D216 has been identified associated with malignant melanoma and MRS2 expression and activity is implicated in gastric cancer. Thus, to gain more mechanistic and functional insight into Mg2+ sensing by the human MRS2 matrix domain and the association with proliferative disease, we assessed the structural, biophysical, and functional effects of a D216Q mutant. We show that the D216Q mutation is sufficient to abrogate Mg2+-binding and associated conformational changes including increased α-helicity, stability, and monomerization. Further, we reveal that the MRS2 matrix domains interact with ~μM affinity, which is weakened by up to two orders of magnitude in the presence of Mg2+ for wild-type but unaffected for D216Q. Finally, we demonstrate the importance of Mg2+ sensing by MRS2 to prevent matrix Mg2+ overload as HeLa cells overexpressing MRS2 show enhanced Mg2+ uptake, cell migration, and resistance to apoptosis while MRS2 D216Q robustly potentiates these cancer phenotypes. Collectively, our findings further define the MRS2 matrix domain as a critical Mg2+ sensor that undergoes conformational and assembly changes upon Mg2+ interactions dependent on D216 to temper matrix Mg2+ overload.","PeriodicalId":20761,"journal":{"name":"Protein Science","volume":"33 8","pages":"e5108"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11237551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MTR3D-AF2: Expanding the coverage of spatially derived missense tolerance scores across the human proteome using AlphaFold2. MTR3D-AF2：使用 AlphaFold2 在人类蛋白质组中扩大空间推导的错义容忍度分数的覆盖范围。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-08-01 DOI: 10.1002/pro.5112

Aaron S Kovacs, Stephanie Portelli, Michael Silk, Carlos H M Rodrigues, David B Ascher

引用次数: 0

Haspin mediates H3.3S31 phosphorylation downstream of Aurora B in mouse embryonic stem cells. Haspin 在小鼠胚胎干细胞中介导极光 B 下游的 H3.3S31 磷酸化。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-08-01 DOI: 10.1002/pro.5126

Yuanyuan Li, Meixian Wu, Yang Liu, Lihua Sun, Peiqiang Mu, Binbin Ma, Jing Xie

{"title":"Haspin mediates H3.3S31 phosphorylation downstream of Aurora B in mouse embryonic stem cells.","authors":"Yuanyuan Li, Meixian Wu, Yang Liu, Lihua Sun, Peiqiang Mu, Binbin Ma, Jing Xie","doi":"10.1002/pro.5126","DOIUrl":"10.1002/pro.5126","url":null,"abstract":"Histone phosphorylation is instrumental in regulating diverse cellular processes across eukaryotes. Unraveling the kinases that target specific histone sites is key to deciphering the underlying mechanisms. Among the various sites on histone tails that can undergo phosphorylation, the kinase responsible for H3.3S31 phosphorylation remained elusive. Since both H3.3S31ph and H3T3ph occur specifically during mitosis, and Haspin is the known kinase for H3T3 phosphorylation, we investigated its potential role in H3.3S31 phosphorylation. We employed CRISPR/Cas9, RNA interference, and specific small molecule inhibitors to eliminate Haspin function in various cell types. Our data consistently revealed a link between Haspin and H3.3S31ph. Furthermore, in vitro kinase assays provided evidence supporting Haspin's contribution to H3.3S31ph. Loss- and gain-of-function experiments targeting Haspin and Aurora B further suggested a hierarchical relationship. Haspin acts as a downstream kinase of Aurora B, specifically orchestrating H3.3S31 phosphorylation in mESCs. This study unveils a novel role for Haspin as a kinase in regulating H3.3S31 phosphorylation during mitosis. This discovery holds promise for expanding our understanding of the functional significance of Haspin and H3.3S31ph in mammals.","PeriodicalId":20761,"journal":{"name":"Protein Science","volume":"33 8","pages":"e5126"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11284449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and biochemical characterization of an encapsulin-associated rhodanese from Acinetobacter baumannii. 鲍曼不动杆菌包囊蛋白相关罗丹的结构和生物化学特征。

IF 4.5 3区生物学

Protein Science Pub Date : 2024-08-01 DOI: 10.1002/pro.5129

Robert Benisch, Tobias W Giessen

{"title":"Structural and biochemical characterization of an encapsulin-associated rhodanese from Acinetobacter baumannii.","authors":"Robert Benisch, Tobias W Giessen","doi":"10.1002/pro.5129","DOIUrl":"10.1002/pro.5129","url":null,"abstract":"Rhodanese-like domains (RLDs) represent a widespread protein family canonically involved in sulfur transfer reactions between diverse donor and acceptor molecules. RLDs mediate these transsulfuration reactions via a transient persulfide intermediate, created by modifying a conserved cysteine residue in their active sites. RLDs are involved in various aspects of sulfur metabolism, including sulfide oxidation in mitochondria, iron-sulfur cluster biogenesis, and thio-cofactor biosynthesis. However, due to the inherent complexity of sulfur metabolism caused by the intrinsically high nucleophilicity and redox sensitivity of thiol-containing compounds, the physiological functions of many RLDs remain to be explored. Here, we focus on a single domain Acinetobacter baumannii RLD (Ab-RLD) associated with a desulfurase encapsulin which is able to store substantial amounts of sulfur inside its protein shell. We determine the 1.6 Å x-ray crystal structure of Ab-RLD, highlighting a homodimeric structure with a number of unusual features. We show through kinetic analysis that Ab-RLD exhibits thiosulfate sulfurtransferase activity with both cyanide and glutathione acceptors. Using native mass spectrometry and in vitro assays, we provide evidence that Ab-RLD can stably carry a persulfide and thiosulfate modification and may employ a ternary catalytic mechanism. Our results will inform future studies aimed at investigating the functional link between Ab-RLD and the desulfurase encapsulin.","PeriodicalId":20761,"journal":{"name":"Protein Science","volume":"33 8","pages":"e5129"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11284452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0